Lanthanide-doped upconversion nanoparticles as nanoprobes for bioimaging

Biomaterials Science, Journal Year: 2024, Volume and Issue: 12(18), P. 4650 - 4663

Published: Jan. 1, 2024

Upconversion nanoparticles (UCNPs) are a class of nanomaterials composed lanthanide ions with great potential for paraclinical applications, especially in laboratory and imaging sciences. UCNPs have tunable optical properties the ability to convert long-wavelength (low energy) excitation light into short-wavelength (high emission ultraviolet (UV)-visible near-infrared (NIR) spectral regions. The core-shell structure can be customized through chemical synthesis meet needs different applications. surface also tailored by conjugating small molecules and/or targeting ligands achieve high specificity selectivity, which indispensable elements biomedical Specifically, coatings enhance water dispersion, biocompatibility, efficiency UCNPs, thereby optimizing their functionality boosting performance. In this context, multimodal provide more accurate

Language: Английский

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Advancements in Single-Molecule Fluorescence Detection Techniques and Their Expansive Applications in Drug Discovery and Neuroscience

Biosensors, Journal Year: 2025, Volume and Issue: 15(5), P. 283 - 283

Published: April 30, 2025

Single-molecule fluorescence technology stands at the forefront of scientific research as a sophisticated tool, pushing boundaries our understanding. This review comprehensively summarizes technological advancements in single-molecule detection, highlighting latest achievements development fluorescent probes, imaging systems, and biosensors. It delves into applications these cutting-edge tools drug discovery neuroscience research, encompassing design monitoring complex delivery elucidation pharmacological mechanisms pharmacokinetics, intricacies neuronal signaling synaptic function, molecular underpinnings neurodegenerative diseases. The exceptional sensitivity demonstrated underscores vast potential modern biomedical heralding its expansion other domains.

Language: Английский

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Quantum Dots for Chemical Metrology

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Language: Английский

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Revealing the Existence of Long-Range Liquid–Liquid Interfacial Potential in Phase-Transfer Processes

The Journal of Physical Chemistry Letters, Journal Year: 2024, Volume and Issue: 15(23), P. 6241 - 6248

Published: June 6, 2024

By employing fluorescence wide-field microscopy and a nanoparticle-based phase transfer catalyst (PTC), consisting of fluorescent silica nanoparticle functionalized with trioctylpropylammonium bromide, we demonstrate that in the presence NaOH, single nanoparticles display subdiffusive motion along axis normal to an aqueous liquid-organic liquid interface. This is because extended interfacial potential shallow well (∼1 kBT) stretches few μm into organic phase, contrast previous molecular dynamics studies reported narrow interfaces on order ∼1 nm. Spontaneous emulsification induced by NaOH results propagation water-in-oil nanoemulsions solvent creates equilibrium hybrid-solvent composition solvates PTC. A greater mobility longer residence time PTC at enhance process catalytic efficiency.

Language: Английский

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Photon upconversion of Nd³⁺-Yb³⁺-Tb³⁺ doped core-shell-shell-shell nanoparticles

CrystEngComm, Journal Year: 2024, Volume and Issue: 26(27), P. 3603 - 3608

Published: Jan. 1, 2024

The NaYF 4 :Yb migration layer minimized the cross-relaxation. Excited energy absorbed by Nd 3+ could sensitize co-doped Yb and activated Tb though among , thereby achieving luminescence of .

Language: Английский

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LRET-Based Simultaneous Detection of Dual miRNAs via Multitrap Optical Tweezers Assisted Suspension Array Tagged by Two Different Luminescent Quenchable UCNPs Combining CRISPR/Cas12a Amplification

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 97(1), P. 602 - 612

Published: Dec. 23, 2024

Nowadays, optical tweezers play a vital role not only in manipulation but also bioassay. As principal trapping objects, microbeads can combine with suspension array technology, amply focused laser beams and adequately concentrated tags contributing to highly sensitive detection. In view of the inefficiency conventional single-trap tweezers, multitrap systems are developed. Here, green- blue-emitting core-shell-shell upconversion nanoparticles (UCNPs) adopted encode determine dual miRNAs, internal shells leading luminescence process facilitate quenching through resonance energy transfer (LRET). Utilizing trans cleavage CRISPR/Cas12a, quenched signals recovered amplified, causing further enhanced detection sensitivity. Ultimately, limits (LOD) 17 22 aM obtained excellent specificities verified. Furthermore, miRNAs from MCF-7, A549, MCF-10A cells extracted detected, results consistent those by PCR. Notably, miR-155 MCF-7 A549 is detectable at single-cell level. Thus, differences measured miRNA levels between imply potential this method discriminate breast cancer epithelial despite difficulty distinguishing different due similar levels.

Language: Английский

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Luminescent photon-upconversion nanoparticles with advanced functionalization for smart sensing and imaging

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(9)

Published: Aug. 21, 2024

Language: Английский

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