In
this
study,
we
describe
a
light-driven
photocatalytic
decarboxylation
of
palmitic
acid
and
related
fatty
acids
using
Chlorella
variabilis
photodecarboxylase
(CvFAP).
By
utilizing
violet
LED
light
(50
W;
397
nm),
achieved
remarkable
conversion
efficiency
99%
within
just
4
minutes,
surpassing
the
previous
79%
in
60
minutes
blue
(300
439
nm).
Importantly,
use
50
W
also
resulted
with
lower
enzyme
photoinactivation
rate
when
compared
to
300
LED.
Comparing
whole-cell
biocatalyst
enzymatic
extract,
found
that
former
demonstrated
superior
catalytic
performance
reduced
susceptibility
photoinactivation.
Furthermore,
through
fed-batch
reactions
three
pulses
13
mM
acid,
production
39
pentadecane
1
hour,
highlighting
promising
strategy
for
enhanced
productivity.
These
findings
represent
significant
advancement
CvFAP
photodecarboxylation
processes,
an
alternative
source,
potential
implications
biofuel
production.
Chemical Reviews,
Год журнала:
2023,
Номер
123(9), С. 5459 - 5520
Опубликована: Апрель 28, 2023
Biocatalysis
has
revolutionized
chemical
synthesis,
providing
sustainable
methods
for
preparing
various
organic
molecules.
In
enzyme-mediated
most
reactions
involve
molecules
operating
from
their
ground
states.
Over
the
past
25
years,
there
been
an
increased
interest
in
enzymatic
processes
that
utilize
electronically
excited
states
accessed
through
photoexcitation.
These
photobiocatalytic
a
diverse
array
of
reaction
mechanisms
are
complementary
to
one
another.
This
comprehensive
review
will
describe
state-of-the-art
strategies
photobiocatalysis
synthesis
until
December
2022.
Apart
reviewing
relevant
literature,
central
goal
this
is
delineate
mechanistic
differences
between
general
employed
field.
We
organize
based
on
relationship
photochemical
step
and
transformations.
The
include
studies,
substrate
scopes,
protein
optimization
strategies.
By
clearly
defining
mechanistically-distinct
chemistry,
we
hope
illuminate
future
synthetic
opportunities
area.
Renewable and Sustainable Energy Reviews,
Год журнала:
2023,
Номер
184, С. 113548 - 113548
Опубликована: Июль 17, 2023
The
rise
in
energy
consumption
and
non-renewable
fossil
fuel
depletion
has
sparked
interest
finding
more
sustainable
alternatives.
Hydrotreated
vegetable
oil
(HVO)
is
a
prime
candidate
to
replace
conventional
hydrocarbon
(HC)
fuels
as
it
renewable
high
calorific
value.
However,
the
existing
processes
convert
HVO
involve
extreme
operating
conditions
expensive
metal
catalysts.
Recently,
photodecarboxylase
derived
from
Chlorella
variabilis
NC64A
(CvFAP)
demonstrated
great
potential
fatty
acid
(FA)
HC
presence
of
blue
light.
This
simple
one-step
process
that
does
not
form
any
intermediates.
review
summarized
findings
related
CvFAP-catalyzed
production,
including
catalytic
mechanism
challenges
scale-up
commercialization.
Current
studies
have
produce
FA
using
CvFAP.
Abstract
The
recent
increase
of
interest
in
photocatalysis
spread
to
biocatalysis
and
triggered
a
rush
for
the
development
light‐dependent
enzyme‐mediated
or
enzyme‐coupled
processes.
After
several
years
intense
research
on
photobiocatalysis,
it
is
time
evaluate
state
field
structured
manner.
In
this
Perspective,
we
suggest
group
photobiocatalysis
into
distinct
disciplines
provide
principal
guidelines
standards
reporting
photobiocatalytic
results
as
well
advice
performing
reactions.
Overall,
assess
that
contributes
diversity
biocatalytic
reactions
while
offering
selectivity
enzymes
photocatalysis.
We
foresee
ongoing
excitement
enzymatic
processes
will
lead
discovery
novel
mechanisms
complement
with
new
bond‐forming
additional
innovative
strategies
utilize
light
possible
benign
energy
source.
Advanced Energy Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 3, 2025
Abstract
The
excessive
use
of
fossil
fuels
has
significantly
increased
environmental
stress,
driving
the
need
for
green,
sustainable
biofuel
alternatives.
Innovations
in
photocatalysis
(PC),
electrocatalysis
(EC),
and
their
synergistic
approaches,
like
photothermal
catalysis
(PTC),
photo‐enzymatic
(PENC),
photoelectrocatalysis
(PEC),
offer
advanced
methods
biomass
conversion
into
biofuels,
surpassing
traditional
limitations.
However,
comprehensive
research
on
these
processes
is
still
lacking.
This
review
aims
to
systematically
analyze
recent
progress
catalytic
strategies
biomass‐to‐biofuel
conversion.
It
first
describes
characteristics,
types,
properties
biofuels.
Then,
it
explores
fundamental
mechanisms
PC,
EC,
combined
technologies.
chemical
pathways
involved
conversion—such
as
transesterification,
esterification,
hydrogenation,
decarboxylation,
bond
cleavage,
cyclization—are
examined.
Efficient
catalyst
design
specific
reactions
factors
influencing
efficiency
rates
are
also
discussed.
Additionally,
this
paper
assesses
impact
economic
benefits
green
technology
production,
offering
a
valuable
reference
energy
application.
addresses
challenges
deployment
production
suggests
future
directions,
aiming
provide
scientific
guidance
technical
support
development
vital
field.
In
summary,
underscores
importance
continued
innovation
promote
solutions.
ACS Catalysis,
Год журнала:
2024,
Номер
14(3), С. 1808 - 1823
Опубликована: Янв. 19, 2024
Chiral
alcohols
are
versatile
building
blocks
and
of
particular
interest
in
the
asymmetric
synthesis
nonracemic
active
pharmaceutical
ingredients,
agrochemicals,
fragrances,
flavors,
natural
products,
etc.
Herein,
we
report
on
a
"one-pot
sequential
two-step"
concurrent
oxidation–reduction
photobiocatalytic
process
to
synthesize
enantiomerically
enriched
alcohols.
In
this
regard,
an
efficient
photocatalytic
system
based
irradiation
with
440
nm
blue
LEDs
presence
9-fluorenone
as
metal-free
photocatalyst
molecular
oxygen
terminal
oxidant
dry
DMSO
hydrogen
peroxide-neutralizing
agent
was
used
oxidize
broad
range
racemic
(hetero)benzylic
into
prochiral
ketones
quantitively
(>99%
conv.).
The
situ
formed
carbonyl
compounds
were
subsequently
converted
corresponding
chiral
via
biocatalytic
transhydrogenation
catalyzed
by
lyophilized
E.
coli
cells
overexpressing
highly
stereoselective
stereocomplementary
recombinant
alcohol
dehydrogenases
(ADHs)
originated
from
Rhodococcus
ruber
(E.
coli/ADH-A)
or
erythropolis
coli/ReADH)
obtain
(S)-alcohols
Lactobacillus
kefir
coli/Lk-ADH)
KRED-110
(R)-alcohols,
respectively.
Overall,
elaborated
deracemization
using
9-fluorenone-O2-blue
LED-DMSO-E.
coli/ADH
carried
out
semipreparative
scale
(0.25
mmol;
63
mM
final
conc.
4
mL)
at
room
temperature
yielded
aryl
82–99.9%
conv.,
up
92%
isolated
yield,
97–99.9%
ee
complementary
chirality.
Frontiers in Catalysis,
Год журнала:
2023,
Номер
3
Опубликована: Апрель 21, 2023
Flow
biocatalysis
is
a
key
enabling
technology
that
increasingly
being
applied
to
wide
array
of
reactions
with
the
aim
achieving
process
intensification,
better
control
biotransformations,
and
minimization
waste
stream.
In
this
mini-review,
selected
applications
flow
preparation
food
ingredients,
APIs
fat-
oil-derived
commodity
chemicals,
covering
period
2020-2022,
are
described.
Journal of Flow Chemistry,
Год журнала:
2023,
Номер
14(1), С. 211 - 218
Опубликована: Окт. 23, 2023
Abstract
Flow
biocatalysis
has
emerged
as
an
empowering
tool
to
boost
the
potential
of
enzymatic
reactions
towards
more
automatized,
sustainable,
and
generally
efficient
synthetic
processes.
In
last
fifteen
years,
increasing
number
biocatalytic
transformations
carried
out
in
continuous
flow
exemplified
benefits
that
this
technology
can
bring
incorporate
into
industrial
operations.
This
perspective
aims
capture
a
nutshell
available
methodologies
for
well
discuss
current
limitations
future
directions
field.
Graphical
abstract
