Label-Free Electrochemical Biosensor Platforms for Cancer Diagnosis: Recent Achievements and Challenges

Biosensors, Год журнала: 2023, Номер 13(3), С. 333 - 333

Опубликована: Март 1, 2023

With its fatal effects, cancer is still one of the most important diseases today’s world. The underlying fact behind this scenario probably due to late diagnosis. That why necessity for detection different types obvious. Cancer studies including diagnosis and therapy have been laborious tasks. Since early significantly affects following steps, very important. Despite researchers’ best efforts, accurate rapid challenging difficult investigate. It known that electrochemical techniques successfully adapted into field. Electrochemical sensor platforms are brought together with excellent selectivity biosensing elements, such as nucleic acids, aptamers or antibodies, put forth successful outputs. One remarkable achievements these biomolecule-attached sensors their lack need additional labeling which bring extra burdens interference effects demanding modification protocols. In review, we aim outline label-free use methods acquire signals. classification sensing generally presented according recognition element, recent by using attractive substrates described in detail. addition, current challenges discussed.

Язык: Английский

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Pyroelectric-Effect-Assisted Near-Infrared-Driven Photoelectrochemical Biosensor Based on Exponential DNA Amplifier for MicroRNA Detection

Analytical Chemistry, Год журнала: 2023, Номер 96(1), С. 581 - 589

Опубликована: Дек. 27, 2023

Although near-infrared responsive photoelectrochemical (PEC) biosensors have less damage to biological components compared UV–visible light, they still reveal an inferior response due the rapid recombination of photogenerated electron–hole. In this study, a near-infrared-driven PEC biosensor is fabricated for microRNA (miRNA) detection via integrating photoelectricity and pyroelectricity. Upon introduction target miRNA-21, exponential DNA amplifier triggered based on enzyme-assisted strand displacement amplification (SDA), releasing multiple Ag2S reporter probes hybridize with capture immobilized CdS-2-mercaptobenzimidazole (2MBI)-modified photoelectrode. As result, under stimulation NIR, photoelectric conversion NPs generates photocurrents. addition, strong hole acceptor ability MBI, pyroelectric effect CdS-2MBI nanocomposites enhanced, which highly pyroelectro-induced charge separation efficiency induces current benefited from spontaneous polarization caused by temperature variation function nanoheaters. Impressively, has achieved sensitive selective determination miRNA-21 limit as low 54 fM. Overall, NIR-driven effects opens up new horizon bioanalysis early disease diagnosis.

Язык: Английский

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Hierarchical Porous Metal‐Organic Framework as Biocatalytic Microreactor for Enzymatic Biofuel Cell‐Based Self‐Powered Biosensing of MicroRNA Integrated with Cascade Signal Amplification

Small, Год журнала: 2023, Номер 19(35)

Опубликована: Апрель 25, 2023

Enzymatic biofuel cells have become powerful tools in biosensing, which however generally suffer from the limited loading efficiency as well low catalytic activity and poor stability of bioenzymes. Herein, hierarchical porous metal-organic frameworks (MOFs) are synthesized using tannic acid (TA) for structural etching, realizes co-encapsulation glucose dehydrogenase (GDH) nicotinamide adenine dinucleotide (NAD+ ) cofactor zeolitic imidazolate framework (ZIF-L) further used biocatalytic microreactors to modify bioanode. In this work, TA-controlled etching can not only expand pore size microreactors, but also achieve reorientation enzymes their lower surface energy form, therefore enhancing biocatalysis cofactor-dependent enzyme. Meanwhile, topological DNA tetrahedron is assembled on acts microRNA-responsive "lock" perform cascade signal amplification exonuclease III-assisted target recycling bioanode hybridization chain reaction (HCR) biocathode. The proposed self-powered biosensor has achieved a detection limit 2 aM (6 copies miRNA-21 5 µL sample), successfully applied identify cancer clinical serums breast patients based different levels miRNA-21, holding great potential accurate disease identification diagnosis.

Язык: Английский

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Metal–organic framework-engineered enzyme/nanozyme composites: Preparation, functionality, and sensing mechanisms

Chemical Engineering Journal, Год журнала: 2024, Номер 496, С. 153884 - 153884

Опубликована: Июль 9, 2024

Язык: Английский

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Mixed-Ligand-Regulated Self-Enhanced Luminous Eu-MOF as an ECL Signal Probe for an Oriented Antibody-Decorated Biosensing Platform

Analytical Chemistry, Год журнала: 2022, Номер 94(37), С. 12852 - 12859

Опубликована: Сен. 8, 2022

The self-luminescence behavior of lanthanide MOFs (Ln-MOFs) due to the unique antenna effect is considered be a promising electrochemiluminescence (ECL) emission for biosensors. It more challenging Ln-MOFs on account difficulty stimulate Ln ions with desired energy-transfer efficiency produce stronger ECL emissions at low potential. Here, guided by second ligand-assisted strategy, we present an efficient self-enhanced luminescence mixed-ligand Eu-MOF as signal probe oriented antibody-decorated biosensing platform detection limit and broad range. Diamino terephthalic acid (NH2-H2BDC) 1,10-phenanthroline (Phen) were selected first ligands, respectively, form highly conjugated structures, well suppress nonradiative energy transfer. Impressively, Phen precisely adjusts gap between triplet ligand excited state Eu3+, realizing self-enhancement Eu-MOF. mixed adjusted molar ratio obtain stable strong lowered triggering potential (0.83 V). In addition, FeCo@CNT features densely active FeCo sites along rich hierarchy conductive carbon nanotube (CNT) network, which efficiently co-reaction accelerator TPA•+ radicals accelerate reduction process achieving amplification. heptapeptide HWRGWVC (HWR) constructed matrix interface that allowed fragment antigen-binding (Fab) regions target specific antigens enhance incubation efficiency. study has gone some way toward designing luminous Eu-MOF, thus giving new fresh impetus develop high-performance emitters biological analysis.

Язык: Английский

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Biometric Photoelectrochemical–Visual Multimodal Biosensor Based on 3D Hollow HCdS@Au Nanospheres Coupled with Target-Induced Ion Exchange Reaction for Antigen Detection

Analytical Chemistry, Год журнала: 2022, Номер 94(41), С. 14492 - 14501

Опубликована: Окт. 4, 2022

Three-dimensional (3D) hollow photoactive nanomaterials can enhance light capture due to the scattering benefiting from unique nanostructures, which contributes decrease in energy loss and electron-hole recombination during process of photoelectric conversion. Herein, a 3D HCdS@Au nanosphere synthesized by templated-assisted method photodeposition is employed construct multimodal sensing platform combining photoelectrochemical (PEC) biosensor with colorimetric analysis photothermal imaging. In presence target carcinoembryonic antigen (CEA), sandwich structure formed on magnetic beads based dual-aptamer recognition, followed initiation rolling circle amplification (RCA) bind numerous CuO-DNA probes. Upon stimulation chlorhydric acidic, large number Cu2+ released CuO, could interact yellow electrode produce dark CuS ion exchange. As result, increased CEA level, photocurrent weakened color interface changed dark, thus facilitates PEC detection CEA. Simultaneously, highly effect achieve qualitative visual using portable infrared thermal imager. This work exhibits an excellent performance for sensitive selective dynamic working range 0.015 2.4 ng/mL limit as low 3.5 pg/mL. Moreover, proposed successfully applied determination human serum, holds great promise accurate biomarkers early diagnosis diseases clinic.

Язык: Английский

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Tunable metal–organic frameworks assist in catalyzing DNAzymes with amplification platforms for biomedical applications

Chemical Society Reviews, Год журнала: 2023, Номер 52(21), С. 7549 - 7578

Опубликована: Янв. 1, 2023

Various binding modes of tunable metal organic frameworks (MOFs) and functional DNAzymes (Dzs) synergistically catalyze the emergence abundant nanoplatforms.

Язык: Английский

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Metal/covalent-organic framework-based biosensors for nucleic acid detection

Coordination Chemistry Reviews, Год журнала: 2023, Номер 491, С. 215249 - 215249

Опубликована: Май 30, 2023

Язык: Английский

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Paper-Based Colorimetric Detection of miRNA-21 Using Pre-Activated Nylon Membrane and Peroxidase-Mimetic Activity of Cysteamine-Capped Gold Nanoparticles

Biosensors, Год журнала: 2023, Номер 13(1), С. 74 - 74

Опубликована: Янв. 1, 2023

Irregular expression of MicroRNA-21 (miRNA-21) is considered as a promising biomarker for early cancer diagnosis. In this paper, new genosensor based on paper and nanozyme activity cysteamine-capped gold nanoparticles (Cys/AuNPs) was developed to detect picomolar concentrations miRNA-21. Such catalyzes the colorimetric reaction hydrogen peroxide (H2O2) 3,3′,5,5′ tetramethylbenzidine (TMB), produce blue color measurable by smartphone. Due their positive charge, Cys/AuNPs were attached negative phosphate groups DNA strand backbone via electrostatic interactions, leading quantitative determination miRNA-21 concentration peroxidase-like Cys/AuNPs. Furthermore, paper-based assay carried out nylon disk devices allow fast immobilization DNAprobe. After performing assay, good linear range observed between 1 pM nM (Y = 0.080 [MiRNA-21]/pM + 13.846, R2 0.993) with detection limit 0.5 pM. The method effective, selective, sensitive detection. application proposed examined in human serum sample, recovery rate 90.0–97.6% obtained showing acceptable accuracy approach.

Язык: Английский

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Ultrasensitive detection of circulating tumor cells via DNA walker driven by a DNA circuit synergized with MOF-on-MOF nanozyme

Chemical Engineering Journal, Год журнала: 2023, Номер 479, С. 147513 - 147513

Опубликована: Ноя. 19, 2023

Язык: Английский

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