Reproductive Toxicology, Год журнала: 2025, Номер 132, С. 108847 - 108847
Опубликована: Фев. 1, 2025
Язык: Английский
Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)
Опубликована: Авг. 21, 2023
Osteoarthritis (OA) is a common degenerative joint disease urgently needing effective treatments. Bone marrow mesenchymal stromal cell-derived exosomes (Exo) are considered good drug carriers whereas they have limitations such as fast clearance and low retention. This study aimed to overcome the of Exo in delivery using multiple strategies. Novel photocrosslinking spherical gelatin methacryloyl hydrogel (GelMA)-encapsulated cartilage affinity WYRGRL (W) peptide-modified engineered were developed for OA treatment performance (W-Exo@GelMA) loaded with small inhibitor LRRK2-IN-1 (W-Exo-L@GelMA) was investigated vitro vivo. The W-Exo-L@GelMA showed an targeting effect on chondrocytes pronounced action suppressing catabolism promoting anabolism vitro. Moreover, remarkably inhibited OA-related inflammation immune gene expression, rescuing IL-1β-induced transcriptomic responses. With enhanced retention joint, demonstrated superior anti-OA activity repair ability murine model. therapeutic validated cultured human cartilage. In conclusion, hydrogel-encapsulated exhibit notable potential therapy. Engineering by series strategies cartilage-targeting Exo-mediated may offer novel strategy treatment.Clinical trial registration: Not applciable.
Язык: Английский
Процитировано
42
Advanced Materials, Год журнала: 2024, Номер 36(33)
Опубликована: Июнь 27, 2024
Extracellular vesicles (EVs) are promising next-generation therapeutics and drug delivery systems due to demonstrated safety efficacy in preclinical models early-stage clinical trials. There is an urgent need address the immunogenicity of EVs (beyond apparent lack immunotoxicity) advance development. To date, few studies have assessed unintended immunological recognition EVs. An in-depth understanding EV-induced clearance necessary develop effective therapeutic strategies, including approaches mitigate when undesired. This article summarizes various factors involved potential strategies reduce for improved benefit.
Язык: Английский
Процитировано
38
ACS Nano, Год журнала: 2024, Номер 18(3), С. 2231 - 2249
Опубликована: Янв. 8, 2024
Rheumatoid arthritis (RA) severely lowers the life quality by progressively destructing joint functions and eventually causing permanent disability, representing a pressing public health concern. The pathogenesis of RA includes excessive production proinflammatory cytokines harmful oxygen-derived free radicals, such as nitric oxide (NO), which constitute vital targets for precise diagnosis effective treatment RA. In this study, we introduce an advanced nanoagent that integrates microenvironment-activatable photoacoustic (PA) imaging with multitarget synergistic A highly sensitive organic probe NO-tunable energy transformation molecular geometry is developed, enables strong near-infrared absorption turn-on PA signal, active intramolecular motion could further boost conversion. coassembled inflammation-responsive prodrug to construct theranostic nanoagent, on macrophage-derived cell membrane natural tropism inflammatory sites camouflaged improve targeting ability inflamed joints. not only sensitively detect differentiate severity but also efficiently alleviate symptoms function. combination activatable probe-mediated NO scavenging on-demand activation anti-inflammatory significantly inhibits factors promotes macrophage repolarization from M1 M2 phenotype. This meticulously designed ingeniously RA-specific therapy, rendering tremendous promise intervention RA-related diseases.
Язык: Английский
Процитировано
19
Advanced Science, Год журнала: 2025, Номер unknown
Опубликована: Янв. 10, 2025
Abstract The infiltration and excessive polarization of M1 macrophages contribute to the induction persistence low‐grade inflammation in joint‐related degenerative diseases such as osteoarthritis (OA). lipid metabolism dysregulation promotes macrophage by coordinating compensatory pathways inflammatory oxidative stress responses. Here, a self‐assembling, licofelone‐loaded nanoparticle (termed LCF‐CSBN), comprising chondroitin sulfate bilirubin joined an ethylenediamine linker, is developed selectively reprogram activation. LCF‐CSBN internalized via CD44‐mediated endocytosis targets Golgi apparatus accompanied with reactive oxygen species‐responsive release licofelone (LCF, dual inhibitor arachidonic acid metabolism). effectively M2 transition reprogramming apparatus‐related sphingolipid metabolism. Intra‐articularly injected retains joint for up 28 days accumulates into macrophages. Moreover, can attenuate inflammation, stress, cartilage degeneration OA model rats. These findings indicate promising potential lipid‐metabolism‐reprogramming targeted therapy OA.
Язык: Английский
Процитировано
2
Gels, Год журнала: 2023, Номер 9(7), С. 533 - 533
Опубликована: Июнь 30, 2023
Injectable hydrogels have gained popularity for their controlled release, targeted delivery, and enhanced mechanical properties. They hold promise in cardiac regeneration, joint diseases, postoperative analgesia, ocular disorder treatment. Hydrogels enriched with nano-hydroxyapatite show potential bone addressing challenges of defects, osteoporosis, tumor-associated regeneration. In wound management cancer therapy, they enable accelerated closure, drug delivery. also find applications ischemic brain injury, tissue cardiovascular personalized immunotherapy. This manuscript highlights the versatility injectable hydrogel nanocomposites biomedical research. Moreover, it includes a perspective section that explores future prospects, emphasizes interdisciplinary collaboration, underscores promising research applications.
Язык: Английский
Процитировано
38
Biomaterials Science, Год журнала: 2023, Номер 12(1), С. 8 - 56
Опубликована: Окт. 25, 2023
Hydrogels have established their significance as prominent biomaterials within the realm of biomedical research. However, injectable hydrogels garnered greater attention compared with conventional counterparts due to excellent minimally invasive nature and adaptive behavior post-injection. With rapid advancement emerging chemistry deepened understanding biological processes, contemporary been endowed an "intelligent" capacity respond various endogenous/exogenous stimuli (such temperature, pH, light magnetic field). This innovation has spearheaded revolutionary transformations across fields such tissue engineering repair, controlled drug delivery, disease-responsive therapies, beyond. In this review, we comprehensively expound upon raw materials (including natural synthetic materials) principles these advanced hydrogels, concurrently providing a detailed discussion prevalent strategies for conferring stimulus responsiveness. Finally, elucidate latest applications "smart" stimuli-responsive in domain, offering insights into prospects.
Язык: Английский
Процитировано
29
ACS Nano, Год журнала: 2023, Номер 17(23), С. 24308 - 24319
Опубликована: Ноя. 17, 2023
Meniscus injuries are associated with the degeneration of cartilage and development osteoarthritis (OA). It is challenging to protect articular improve exercise when a meniscus injury occurs. Herein, inspired by components functions meniscus, we developed self-lubricating friction-responsive hydrogel that contains nanoliposomes loaded diclofenac sodium (DS) Kartogenin (KGN) for anti-inflammation regeneration. When was injected into site, drug-loaded were released from in manner reassembled form hydration layers lubricate joints during movement. Meanwhile, DS KNG constantly mitigate inflammation promote Additionally, this exhibited favorable injectability, mechanical properties, fatigue resistance, prolonged degradation. In vivo experiments demonstrated injection effectively improved performance protected rats, suggesting it as potential therapeutic approach meniscal injuries.
Язык: Английский
Процитировано
25
Advanced Functional Materials, Год журнала: 2023, Номер 33(51)
Опубликована: Авг. 11, 2023
Abstract The high density structure of cartilage matrix negative charge seriously hinders the penetration and enrichment drugs/genes into tissue. In this study, hexachlorocyclotriphosphonitrile is used as core to prepare self‐polymerized phosphorus dendrimers with inflammatory reaction through substitution condensation reaction. vector can achieve efficient damaged articular (slightly acidic condition) effective anchoring (normal physiological size effect under response. Meanwhile, a gene “delivery library,” G1‐NC5.HCl@siRNA gradually released microspheres degrading; long‐term silencing specific genes realized. Further, loaded hydrogel by ionic bond coordination microfluidic techniques improve residency in joint cavity. results vitro vivo e xperiments show that complex has better at pH = 6.6, decreases 7.6, which effectively anchor site for continuous drug delivery. composite significantly degeneration osteoarthritis (OA) promote regeneration. Therefore, microsphere delivery system response promising penetrant/anchoring carrier OA treatment.
Язык: Английский
Процитировано
23
International Journal of Nanomedicine, Год журнала: 2023, Номер Volume 18, С. 4969 - 4983
Опубликована: Сен. 1, 2023
Abstract: Osteoarthritis (OA) is a prevalent global health concern, posing significant and increasing public challenge worldwide. Recently, nanotechnology-boosted biomaterials have emerged as highly promising strategy for OA therapy due to their exceptional physicochemical properties capacity regulate pathological processes. However, there an urgent need deeper understanding of the potential therapeutic applications these in clinical management diseases, particularly treatment OA. In this comprehensive review, we present extensive discussion current status future prospects concerning therapy. Initially, discuss pathophysiology constraints associated with existing modalities. Subsequently, various types nanomaterials utilized therapy, including nanoparticles, nanofibers, nanocomposites, are thoroughly discussed summarized, elucidating respective advantages challenges. Furthermore, analyze recent preclinical studies that highlight Additionally, research directions evolving field highlighted. By establishing link between structural functions treatment, aim foster advances designing sophisticated OA, ultimately resulting improved efficacy through translation into setting near future. Keywords: nanotechnology, nanomaterials, osteoarthritis, cartilage, RANKL
Язык: Английский
Процитировано
22
Biomedicines, Год журнала: 2024, Номер 12(4), С. 923 - 923
Опубликована: Апрель 22, 2024
This review critically evaluates advancements in multifunctional hydrogels, particularly focusing on their applications osteoarthritis (OA) therapy. As research evolves from traditional natural materials, there is a significant shift towards synthetic and composite known for superior mechanical properties enhanced biodegradability. spotlights novel such as injectable microneedle technology, responsive which have revolutionized OA treatment through targeted efficient therapeutic delivery. Moreover, it discusses innovative hydrogel including protein-based superlubricating potential to reduce joint friction inflammation. The integration of bioactive compounds within hydrogels augment efficacy also examined. Furthermore, the anticipates continued technological deeper understanding hydrogel-based therapies. It emphasizes provide tailored, minimally invasive treatments, thus highlighting critical role advancing dynamic field biomaterial science management.
Язык: Английский
Процитировано
14