Опубликована: Янв. 1, 2024
Язык: Английский
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Янв. 16, 2025
RNA interference (RNAi) and oxidative stress inhibition therapeutic strategies have been extensively utilized in the treatment of osteoarthritis (OA), most prevalent degenerative joint disease. However, synergistic effects these approaches on attenuating OA progression remain largely unexplored. In this study, matrix metalloproteinase-13 siRNA (siMMP-13) was incorporated onto polyethylenimine (PEI)-polyethylene glycol (PEG) modified Fe3O4 nanoparticles, forming a nucleic acid nanocarrier termed si-Fe NPs. Subsequently, poly(vinyl alcohol) (PVA) crosslinked phenylboronic (PBA)-modified hyaluronic (HA) hydrogel (HPP) used to encapsulate NPs, resulting bifunctional (si-Fe-HPP) with reactive oxygen species (ROS)-responsive RNAi properties. Studies vitro demonstrated that si-Fe-HPP exhibited excellent biocompatibility, anti-inflammatory prolonged stable retention time knee joint. Intra-articular injection significantly attenuated cartilage degradation mice destabilization medial meniscus (DMM)-induced OA. The not only notably alleviated synovitis, osteophyte formation subchondral bone sclerosis, but also markedly improved physical activity reduced pain DMM-induced mice. This study reveals si-Fe-HPP, its ROS-responsive abilities, can protect chondrocytes attenuate progression, providing novel insights directions for development materials treatment.
Язык: Английский
Процитировано
4
Journal of Colloid and Interface Science, Год журнала: 2025, Номер 686, С. 498 - 508
Опубликована: Янв. 31, 2025
Язык: Английский
Процитировано
3
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Март 6, 2025
Knee osteoarthritis is a common degenerative joint disease involving multiple pathological processes, including energy metabolism, cartilage repair, and osteogenesis. To investigate the alterations in critical metabolic pathways differential proteins patients through metabolomic proteomic analyses to explore potential mechanisms underlying synovial osteogenesis during progression. Metabolomics was used analyze metabolites fluid synovium of (osteoarthritis group: 10; control 10), whereas proteomics examine protein expression. Alkaline phosphatase activity assessed evaluate Upregulation tricarboxylic acid cycle: Significant upregulation cycle indicated increased metabolism repair activity. Arginine collagen degradation: Elevated levels ornithine, proline, hydroxyproline reflect active degradation contributing breakdown. Abnormal Phenylalanine Metabolism: Increased phenylalanine tyrosine metabolite suggest their involvement destruction Synovial osteogenesis: expression type I elevated alkaline confirmed occurrence osteogenesis, potentially driven by differentiation fibroblasts, mesenchymal stem cells, hypertrophic chondrocytes. Relationships between FN1 TGFBI are closely associated with while provides source for osteogenic transformation. Alterations critical. The related have as diagnostic therapeutic targets osteoarthritis.
Язык: Английский
Процитировано
3
Pharmaceuticals, Год журнала: 2024, Номер 17(12), С. 1711 - 1711
Опубликована: Дек. 18, 2024
Nanotechnology has revolutionised biomedical research, offering innovative healthcare solutions. Plant-based nanotechnology is emerging as a sustainable alternative, minimising environmental impacts and enhancing therapeutic effectiveness. This paper explores the potential of plant-derived nanoparticles (PNPs) in medicine, highlighting their biocompatibility, multifunctionality, eco-friendliness. PNPs, synthesised through green methods, have demonstrated promising applications drug delivery, cancer therapy, antimicrobial treatments, tissue regeneration. Their unique properties, such high surface area bioactive components, enable improved targeting, controlled release, reducing side effects treatment efficacy. Additionally, compounds’ inherent antioxidant retained within platinum (PNPs), present opportunities for combating resistance promoting wound healing. Despite potential, challenges remain standardising PNP synthesis, ensuring consistency, scaling up production industrial applications. review emphasises need further research on toxicity, regulatory frameworks to fully harness capabilities clinical commercial represents promising, greener alternative advancing solutions, aligning with global sustainability goals.
Язык: Английский
Процитировано
14
Military Medical Research, Год журнала: 2024, Номер 11(1)
Опубликована: Окт. 21, 2024
Abstract Severe tissue defects present formidable challenges to human health, persisting as major contributors mortality rates. The complex pathological microenvironment, particularly the disrupted immune landscape within these defects, poses substantial hurdles existing regeneration strategies. However, emergence of nanobiotechnology has opened a new direction in immunomodulatory nanomedicine, providing encouraging prospects for and restoration. This review aims gather recent advances nanomedicine foster regeneration. We begin by elucidating distinctive features local microenvironment defective tissues its crucial role Subsequently, we explore design functional properties nanosystems. Finally, address clinical translation development, aiming propose potent approach enhance through synergistic modulation integration.
Язык: Английский
Процитировано
10
Drug Delivery and Translational Research, Год журнала: 2025, Номер unknown
Опубликована: Янв. 29, 2025
Язык: Английский
Процитировано
2
Acta Biomaterialia, Год журнала: 2025, Номер unknown
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
1
Nano Today, Год журнала: 2025, Номер 61, С. 102664 - 102664
Опубликована: Фев. 12, 2025
Язык: Английский
Процитировано
1
Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160557 - 160557
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
1
ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown
Опубликована: Март 17, 2025
The progression of osteoarthritis (OA) is closely linked to synovial inflammation caused by an imbalance between M1 and M2 macrophages. To tackle this problem, we developed a liposome responsive reactive oxygen species (ROS), modified with folic acid ligands target M1-polarized macrophages, loaded the anti-inflammatory agent dimethyl fumarate (DMF). This liposome-based drug delivery system was designed reprogram macrophage phenotype remodel inflammatory microenvironment in joint cavity alleviate OA degeneration. prepared had suitable size negative zeta potential, uniform size, good stability aqueous solution, excellent biocompatibility. Laboratory tests showed that these DMF-filled liposomes notably decreased high levels ROS M1-type macrophages shifted polarization via Nrf2/HO-1 pathway, which turn lessened chondrocytes averted their apoptosis. Additionally, animal studies demonstrated containing DMF exhibited notable properties, significantly reduced inflammation, safeguarded injured cartilage, reversed changes subchondral bone, effectively slowed mouse model induced anterior cruciate ligament transection (ACLT). Therefore, ROS-responsive targeting represent promising valuable approach for treatment.
Язык: Английский
Процитировано
1