A ROS-responsive hydrogel encapsulated with matrix metalloproteinase-13 siRNA nanocarriers to attenuate osteoarthritis progression

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 16, 2025

RNA interference (RNAi) and oxidative stress inhibition therapeutic strategies have been extensively utilized in the treatment of osteoarthritis (OA), most prevalent degenerative joint disease. However, synergistic effects these approaches on attenuating OA progression remain largely unexplored. In this study, matrix metalloproteinase-13 siRNA (siMMP-13) was incorporated onto polyethylenimine (PEI)-polyethylene glycol (PEG) modified Fe3O4 nanoparticles, forming a nucleic acid nanocarrier termed si-Fe NPs. Subsequently, poly(vinyl alcohol) (PVA) crosslinked phenylboronic (PBA)-modified hyaluronic (HA) hydrogel (HPP) used to encapsulate NPs, resulting bifunctional (si-Fe-HPP) with reactive oxygen species (ROS)-responsive RNAi properties. Studies vitro demonstrated that si-Fe-HPP exhibited excellent biocompatibility, anti-inflammatory prolonged stable retention time knee joint. Intra-articular injection significantly attenuated cartilage degradation mice destabilization medial meniscus (DMM)-induced OA. The not only notably alleviated synovitis, osteophyte formation subchondral bone sclerosis, but also markedly improved physical activity reduced pain DMM-induced mice. This study reveals si-Fe-HPP, its ROS-responsive abilities, can protect chondrocytes attenuate progression, providing novel insights directions for development materials treatment.

Language: Английский

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Electrosprayed core–shell microspheres co-deliver fibronectin and resveratrol for combined treatment of acute lung injury

Journal of Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 686, P. 498 - 508

Published: Jan. 31, 2025

Language: Английский

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Multi-omics analysis of synovial tissue and fluid reveals differentially expressed proteins and metabolites in osteoarthritis

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 6, 2025

Knee osteoarthritis is a common degenerative joint disease involving multiple pathological processes, including energy metabolism, cartilage repair, and osteogenesis. To investigate the alterations in critical metabolic pathways differential proteins patients through metabolomic proteomic analyses to explore potential mechanisms underlying synovial osteogenesis during progression. Metabolomics was used analyze metabolites fluid synovium of (osteoarthritis group: 10; control 10), whereas proteomics examine protein expression. Alkaline phosphatase activity assessed evaluate Upregulation tricarboxylic acid cycle: Significant upregulation cycle indicated increased metabolism repair activity. Arginine collagen degradation: Elevated levels ornithine, proline, hydroxyproline reflect active degradation contributing breakdown. Abnormal Phenylalanine Metabolism: Increased phenylalanine tyrosine metabolite suggest their involvement destruction Synovial osteogenesis: expression type I elevated alkaline confirmed occurrence osteogenesis, potentially driven by differentiation fibroblasts, mesenchymal stem cells, hypertrophic chondrocytes. Relationships between FN1 TGFBI are closely associated with while provides source for osteogenic transformation. Alterations critical. The related have as diagnostic therapeutic targets osteoarthritis.

Language: Английский

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Nanotechnology for Healthcare: Plant-Derived Nanoparticles in Disease Treatment and Regenerative Medicine

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(12), P. 1711 - 1711

Published: Dec. 18, 2024

Nanotechnology has revolutionised biomedical research, offering innovative healthcare solutions. Plant-based nanotechnology is emerging as a sustainable alternative, minimising environmental impacts and enhancing therapeutic effectiveness. This paper explores the potential of plant-derived nanoparticles (PNPs) in medicine, highlighting their biocompatibility, multifunctionality, eco-friendliness. PNPs, synthesised through green methods, have demonstrated promising applications drug delivery, cancer therapy, antimicrobial treatments, tissue regeneration. Their unique properties, such high surface area bioactive components, enable improved targeting, controlled release, reducing side effects treatment efficacy. Additionally, compounds’ inherent antioxidant retained within platinum (PNPs), present opportunities for combating resistance promoting wound healing. Despite potential, challenges remain standardising PNP synthesis, ensuring consistency, scaling up production industrial applications. review emphasises need further research on toxicity, regulatory frameworks to fully harness capabilities clinical commercial represents promising, greener alternative advancing solutions, aligning with global sustainability goals.

Language: Английский

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Microenvironment-responsive nanomedicines: a promising direction for tissue regeneration

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: Oct. 21, 2024

Abstract Severe tissue defects present formidable challenges to human health, persisting as major contributors mortality rates. The complex pathological microenvironment, particularly the disrupted immune landscape within these defects, poses substantial hurdles existing regeneration strategies. However, emergence of nanobiotechnology has opened a new direction in immunomodulatory nanomedicine, providing encouraging prospects for and restoration. This review aims gather recent advances nanomedicine foster regeneration. We begin by elucidating distinctive features local microenvironment defective tissues its crucial role Subsequently, we explore design functional properties nanosystems. Finally, address clinical translation development, aiming propose potent approach enhance through synergistic modulation integration.

Language: Английский

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pH-sensitive nano-drug delivery systems dual-target endothelial cells and macrophages for enhanced treatment of atherosclerosis

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Language: Английский

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Bioactive Phosphorus Dendrimers Deliver Protein/Drug to Tackle Osteoarthritis via Cooperative Macrophage Reprogramming

Biomaterials, Journal Year: 2024, Volume and Issue: 316, P. 122999 - 122999

Published: Dec. 5, 2024

Language: Английский

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Design and advances in antioxidant hydrogels for ROS-induced oxidative disease

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Codelivery of fibronectin and rapamycin via bioactive phosphorus dendrimers to ameliorate Alzheimer’s disease through macrophage autophagy, oxidative stress alleviation and polarization modulation

Nano Today, Journal Year: 2025, Volume and Issue: 61, P. 102664 - 102664

Published: Feb. 12, 2025

Language: Английский

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Implant coating for promoted postoperative bone regeneration via cascade Reaction-Mediated microenvironment reprogramming

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160557 - 160557

Published: Feb. 1, 2025

Language: Английский

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