Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 26, 2025
Abstract
Drug‐induced
liver
injury
(DILI)
is
a
common
clinical
problem
with
urgent
respect
to
demanding
early
diagnosis.
Exosomal
miRNAs
are
reliable
and
noninvasive
biomarkers
for
the
diagnosis
of
DILI.
However,
accurate
feasible
detection
exosomal
often
hampered
by
low
abundance
miRNAs,
inefficient
exosome
separation
techniques,
requirement
RNA
extraction
from
large
sample
volumes.
Here,
multifunctional
magnetic
vesicles
constructed
loading
multiple
signal
amplification
system
nanoparticles
into
virus‐mimicking
engineered
achieve
in
situ
analysis
hepatogenic
which
do
not
require
miRNA
or
target
amplification.
Virus‐mimicking
carrying
surface
proteins
hepatitis
B
virus
designed
specific
identity
fusion
exosomes,
assembled
catalytic
hairpin
assembly
technology
CRISPR/Cas13a
can
highly
sensitive
exosomes
limit
(LOD)
1.25
×
10
2
particles·µL
−1
.
This
novel
nanoplatforms
open
promising
avenue
MicroRNAs
(miRNAs)
in
extracellular
vesicles
(EVs)
play
essential
roles
cancer
initiation
and
progression.
Quantitative
measurements
of
EV
miRNAs
are
critical
for
diagnosis
longitudinal
monitoring.
Traditional
PCR-based
methods,
however,
require
multi-step
procedures
remain
as
bulk
analysis.
Here,
the
authors
introduce
an
amplification-free
extraction-free
miRNA
detection
method
using
a
CRISPR/Cas13a
sensing
system.
components
encapsulated
liposomes
delivered
them
into
EVs
through
liposome-EV
fusion.
This
allows
accurately
quantify
specific
miRNA-positive
counts
1
×
108
EVs.
The
show
that
miR-21-5p-positive
range
2%-10%
ovarian
EVs,
which
is
significantly
higher
than
positive
from
benign
cells
(<0.65%).
result
excellent
correlation
between
analysis
with
gold-standard
method,
RT-qPCR.
also
demonstrate
multiplexed
protein-miRNA
tumor-derived
by
capturing
EpCAM-positive
quantifying
ones
subpopulation,
plasma
patients
healthy
controls.
developed
system
provides
intact
without
RNA
extraction
opens
up
possibility
single
protein
markers.
Pharmaceutics,
Год журнала:
2024,
Номер
16(3), С. 400 - 400
Опубликована: Март 14, 2024
Lipid-bilayer-based
liposomes
are
gaining
attention
in
scientific
research
for
their
versatile
role
drug
delivery.
With
amphiphilic
design,
efficiently
encapsulate
and
deliver
drugs
to
targeted
sites,
offering
controlled
release.
These
artificial
structures
hold
great
promise
advancing
cancer
therapy
methodologies.
Bibliometric
analyzes
systematic
literary
data
statistically.
This
study
used
bibliometric
indicators
examine,
map,
evaluate
the
applications
of
therapy.
A
Scopus
search
was
conducted
identify
all
English-language
peer-reviewed
publications
on
within
past
twenty
years.
were
calculated
using
VOSviewer
Biblioshiny.
We
produced
thematic,
conceptual,
visualization
charts.
total
14,873
published
documents
obtained.
The
procedure
keyword
mapping
has
effectively
identified
main
areas
concentration
prevailing
trends
this
specific
field
study.
significant
clusters
discovered
through
theme
hotspot
analyses
encompassed
many
topics
such
as
use
multiple
strategies
chemotherapy
different
forms
cancer,
pharmacokinetics
nanomedicine,
well
investigation
delivery,
cytotoxicity,
gene
Liposomes
employed
delivery
systems
so
selectively
target
cells
improve
bioavailability
anticancer
drugs.
work
showcased
capacity
tailor
these
accurate
by
including
potent
medications.
Our
findings
not
only
bring
latest
progress
utilizing
treatment
but
also
underscore
vital
need
ongoing
research,
collaborative
efforts,
effective
translation
breakthroughs
into
tangible
clinical
applications,
emphasizing
dynamic
evolving
nature
therapeutics.
Abstract
The
advent
of
Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
(CRISPR)‐based
genome
editing
technologies
has
opened
up
groundbreaking
possibilities
for
treating
a
wide
spectrum
genetic
disorders
and
diseases.
However,
the
success
these
relies
heavily
on
development
efficient
safe
delivery
systems.
Among
most
promising
approaches
are
natural
synthetic
nanocarrier‐mediated
systems,
including
viral
vectors,
extracellular
vesicles
(EVs),
engineered
cellular
membrane
particles,
liposomes,
various
nanoparticles.
These
carriers
enhance
efficacy
CRISPR
system
by
providing
unique
combination
efficiency,
specificity,
reduced
immunogenicity.
Synthetic
such
as
liposomes
nanoparticles
facilitate
with
high
reproducibility
customizable
functions.
Viral
renowned
their
transduction
efficiency
broad
tropism,
serve
powerful
vehicles
delivering
components
to
cell
types.
EVs,
RNA
proteins,
offer
stealth
mechanism
evade
immune
detection,
allowing
targeted
editors
minimal
off‐target
effects.
Engineered
particles
further
improve
simulating
environment,
enhancing
uptake,
minimizing
response.
This
review
explores
innovative
integration
nanocarrier
focusing
recent
advancements,
applications,
future
directions
in
therapeutic
editing.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 9, 2025
Early
and
precise
diagnosis
of
neurodegenerative
disorders
like
Alzheimer's
(AD)
Parkinson's
(PD)
is
crucial
for
slowing
their
progression
enhancing
patient
outcomes.
Exosomal
microRNAs
(miRNAs)
are
emerging
as
promising
biomarkers
due
to
ability
reflect
the
diseases'
pathology,
yet
low
abundance
poses
significant
detection
hurdles.
This
review
article
delves
into
burgeoning
field
electrochemical
biosensors,
designed
exosomal
miRNA
biomarkers.
Electrochemical
biosensors
offer
a
compelling
solution,
combining
sensitivity
required
detect
low-abundance
with
specificity
needed
discern
profiles
distinctive
neural
pathological
states.
We
explore
operational
principles
these
including
transduction
mechanisms
that
facilitate
detection.
The
also
summarizes
advancements
in
nanotechnology,
signal
enhancement,
bioreceptor
anchoring,
microfluidic
integration
improve
sensor
accuracy.
evidence
use
disease
analyzed,
focusing
on
clinical
impact,
diagnostic
precision,
obstacles
faced
practical
applications.
Their
potential
point-of-care
testing
regulatory
considerations
market
entry
discussed.
Looking
toward
future,
highlights
forthcoming
innovations
might
revolutionize
early
processes.
impressive
sensitivity,
specificity,
compatibility,
track
become
instrumental
diseases,
possibly
transforming
care
prognosis.
Advanced Drug Delivery Reviews,
Год журнала:
2023,
Номер
200, С. 115028 - 115028
Опубликована: Июль 28, 2023
Lipid-based
nanocarriers
have
been
extensively
investigated
for
their
application
in
drug
delivery.
Particularly,
liposomes
are
now
clinically
established
treating
various
diseases
such
as
fungal
infections.
In
contrast,
extracellular
vesicles
(EVs)
-
small
cell-derived
nanoparticles
involved
cellular
communication
just
recently
sparked
interest
carriers
but
development
is
still
at
the
preclinical
level.
To
drive
this
further,
methods
and
technologies
exploited
context
of
liposome
research
should
be
applied
domain
EVs
to
facilitate
accelerate
clinical
translation.
One
crucial
steps
EV-based
therapeutics
designing
them
proper
dosage
forms
specific
applications.
This
review
offers
a
comprehensive
overview
state-of-the-art
polysaccharide-based
hydrogel
platforms
designed
artificial
natural
with
delivery
skin.
We
discuss
physicochemical
biological
properties
try
create
sound
basis
optimization
EV-embedded
hydrogels
versatile
therapeutic
avenues.
Chemical Communications,
Год журнала:
2024,
Номер
60(17), С. 2301 - 2319
Опубликована: Янв. 1, 2024
This
feature
article
summarises
the
latest
advances,
potential
challenges,
and
future
prospects
of
CRISPR/Cas
gene-editing
technologies
in
conjunction
with
emerging
nanodevices
for
refinement
precision
liquid
biopsy
techniques.
Abstract
Exosomes
are
indispensable
for
intercellular
communications.
Tumor
microenvironment
(TME)
is
the
living
environment
of
tumor
cells,
which
composed
various
components,
including
immune
cells.
Based
on
TME,
immunotherapy
has
been
recently
developed
eradicating
cancer
cells
by
reactivating
antitumor
effect
The
communications
between
and
TME
crucial
development,
metastasis,
drug
resistance.
play
an
important
role
in
mediating
these
regulating
reprogramming
affects
sensitivity
immunotherapy.
Therefore,
it
imperative
to
investigate
exosomes
impact
Here,
we
review
communication
remodeling
efficacy
immunotherapy,
as
well
summarize
underlying
mechanisms.
Furthermore,
also
introduce
potential
application
artificially
modified
delivery
systems
drugs.
Further
efforts
this
field
will
provide
new
insights
roles
progression,
thus
helping
us
uncover
effective
strategies
treatment.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Окт. 8, 2024
Liquid
biopsy
is
a
minimally
invasive
method
that
uses
biofluid
samples
instead
of
tissue
for
cancer
diagnosis.
Exosomes
are
small
extracellular
vesicles
secreted
by
donor
cells
and
act
as
mediators
intercellular
communication
in
human
health
disease.
Due
to
their
important
roles,
exosomes
have
been
considered
promising
biomarkers
liquid
biopsy.
However,
traditional
methods
exosome
isolation
cargo
detection
time-consuming
inefficient,
limiting
practical
application.
In
the
past
decades,
many
new
strategies,
such
microfluidic
chips,
nanowire
arrays
electrochemical
biosensors,
proposed
achieve
rapid,
accurate
high-throughput
analysis
exosomes.
this
review,
we
discussed
about
advance
exosome-based
technology,
including
isolation,
enrichment,
approaches.
The
comparison
currently
available
also
included.
Finally,
summarized
advantages
limitations
present
strategies
further
gave
perspective
future
translational
use.
