Selective In Situ Analysis of Hepatogenic Exosomal microRNAs via Virus‐Mimicking Multifunctional Magnetic Vesicles DOI Open Access
Lingchang Meng, Lulu Wang, Zhiting Sun

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 26, 2025

Abstract Drug‐induced liver injury (DILI) is a common clinical problem with urgent respect to demanding early diagnosis. Exosomal miRNAs are reliable and noninvasive biomarkers for the diagnosis of DILI. However, accurate feasible detection exosomal often hampered by low abundance miRNAs, inefficient exosome separation techniques, requirement RNA extraction from large sample volumes. Here, multifunctional magnetic vesicles constructed loading multiple signal amplification system nanoparticles into virus‐mimicking engineered achieve in situ analysis hepatogenic which do not require miRNA or target amplification. Virus‐mimicking carrying surface proteins hepatitis B virus designed specific identity fusion exosomes, assembled catalytic hairpin assembly technology CRISPR/Cas13a can highly sensitive exosomes limit (LOD) 1.25 × 10 2 particles·µL −1 . This novel nanoplatforms open promising avenue

Language: Английский

CRISPR/Cas13a‐Based MicroRNA Detection in Tumor‐Derived Extracellular Vesicles DOI Creative Commons
Jae‐Sang Hong, Taehwang Son, Cesar M. Castro

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(24)

Published: June 20, 2023

MicroRNAs (miRNAs) in extracellular vesicles (EVs) play essential roles cancer initiation and progression. Quantitative measurements of EV miRNAs are critical for diagnosis longitudinal monitoring. Traditional PCR-based methods, however, require multi-step procedures remain as bulk analysis. Here, the authors introduce an amplification-free extraction-free miRNA detection method using a CRISPR/Cas13a sensing system. components encapsulated liposomes delivered them into EVs through liposome-EV fusion. This allows accurately quantify specific miRNA-positive counts 1 × 108 EVs. The show that miR-21-5p-positive range 2%-10% ovarian EVs, which is significantly higher than positive from benign cells (<0.65%). result excellent correlation between analysis with gold-standard method, RT-qPCR. also demonstrate multiplexed protein-miRNA tumor-derived by capturing EpCAM-positive quantifying ones subpopulation, plasma patients healthy controls. developed system provides intact without RNA extraction opens up possibility single protein markers.

Language: Английский

Citations

46
Liposome-Based Drug Delivery Systems in Cancer Research: An Analysis of Global Landscape Efforts and Achievements DOI Creative Commons
Islam Hamad, Amani A. Harb, Yasser Bustanji

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(3), P. 400 - 400

Published: March 14, 2024

Lipid-bilayer-based liposomes are gaining attention in scientific research for their versatile role drug delivery. With amphiphilic design, efficiently encapsulate and deliver drugs to targeted sites, offering controlled release. These artificial structures hold great promise advancing cancer therapy methodologies. Bibliometric analyzes systematic literary data statistically. This study used bibliometric indicators examine, map, evaluate the applications of therapy. A Scopus search was conducted identify all English-language peer-reviewed publications on within past twenty years. were calculated using VOSviewer Biblioshiny. We produced thematic, conceptual, visualization charts. total 14,873 published documents obtained. The procedure keyword mapping has effectively identified main areas concentration prevailing trends this specific field study. significant clusters discovered through theme hotspot analyses encompassed many topics such as use multiple strategies chemotherapy different forms cancer, pharmacokinetics nanomedicine, well investigation delivery, cytotoxicity, gene Liposomes employed delivery systems so selectively target cells improve bioavailability anticancer drugs. work showcased capacity tailor these accurate by including potent medications. Our findings not only bring latest progress utilizing treatment but also underscore vital need ongoing research, collaborative efforts, effective translation breakthroughs into tangible clinical applications, emphasizing dynamic evolving nature therapeutics.

Language: Английский

Citations

21
Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing DOI Creative Commons
Elizabeth Maria Clarissa, Mamata Karmacharya,

Hyun‐Min Choi

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Abstract The advent of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)‐based genome editing technologies has opened up groundbreaking possibilities for treating a wide spectrum genetic disorders and diseases. However, the success these relies heavily on development efficient safe delivery systems. Among most promising approaches are natural synthetic nanocarrier‐mediated systems, including viral vectors, extracellular vesicles (EVs), engineered cellular membrane particles, liposomes, various nanoparticles. These carriers enhance efficacy CRISPR system by providing unique combination efficiency, specificity, reduced immunogenicity. Synthetic such as liposomes nanoparticles facilitate with high reproducibility customizable functions. Viral renowned their transduction efficiency broad tropism, serve powerful vehicles delivering components to cell types. EVs, RNA proteins, offer stealth mechanism evade immune detection, allowing targeted editors minimal off‐target effects. Engineered particles further improve simulating environment, enhancing uptake, minimizing response. This review explores innovative integration nanocarrier focusing recent advancements, applications, future directions in therapeutic editing.

Language: Английский

Citations

4
Electrochemical Biosensors for the Detection of Exosomal microRNA Biomarkers for Early Diagnosis of Neurodegenerative Diseases DOI Creative Commons

Jiacheng Yu,

Runzhi Zhou,

Shan Liu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 9, 2025

Early and precise diagnosis of neurodegenerative disorders like Alzheimer's (AD) Parkinson's (PD) is crucial for slowing their progression enhancing patient outcomes. Exosomal microRNAs (miRNAs) are emerging as promising biomarkers due to ability reflect the diseases' pathology, yet low abundance poses significant detection hurdles. This review article delves into burgeoning field electrochemical biosensors, designed exosomal miRNA biomarkers. Electrochemical biosensors offer a compelling solution, combining sensitivity required detect low-abundance with specificity needed discern profiles distinctive neural pathological states. We explore operational principles these including transduction mechanisms that facilitate detection. The also summarizes advancements in nanotechnology, signal enhancement, bioreceptor anchoring, microfluidic integration improve sensor accuracy. evidence use disease analyzed, focusing on clinical impact, diagnostic precision, obstacles faced practical applications. Their potential point-of-care testing regulatory considerations market entry discussed. Looking toward future, highlights forthcoming innovations might revolutionize early processes. impressive sensitivity, specificity, compatibility, track become instrumental diseases, possibly transforming care prognosis.

Language: Английский

Citations

2
Polysaccharide hydrogel platforms as suitable carriers of liposomes and extracellular vesicles for dermal applications DOI Creative Commons
Leila Pourtalebi Jahromi,

Markus Rothammer,

Gregor Fuhrmann

et al.

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 200, P. 115028 - 115028

Published: July 28, 2023

Lipid-based nanocarriers have been extensively investigated for their application in drug delivery. Particularly, liposomes are now clinically established treating various diseases such as fungal infections. In contrast, extracellular vesicles (EVs) - small cell-derived nanoparticles involved cellular communication just recently sparked interest carriers but development is still at the preclinical level. To drive this further, methods and technologies exploited context of liposome research should be applied domain EVs to facilitate accelerate clinical translation. One crucial steps EV-based therapeutics designing them proper dosage forms specific applications. This review offers a comprehensive overview state-of-the-art polysaccharide-based hydrogel platforms designed artificial natural with delivery skin. We discuss physicochemical biological properties try create sound basis optimization EV-embedded hydrogels versatile therapeutic avenues.

Language: Английский

Citations

28
Advances in liposome-based delivery of RNA therapeutics for cancer treatment DOI
Mehdi Sanati, Amir R. Afshari,

Seyed Sajad Ahmadi

et al.

Progress in molecular biology and translational science, Journal Year: 2024, Volume and Issue: unknown, P. 177 - 218

Published: Jan. 1, 2024

Language: Английский

Citations

10
CRISPR/Cas detection with nanodevices: moving deeper into liquid biopsy DOI
Huimin Kong, Ke Yi, Rachel L. Mintz

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(17), P. 2301 - 2319

Published: Jan. 1, 2024

This feature article summarises the latest advances, potential challenges, and future prospects of CRISPR/Cas gene-editing technologies in conjunction with emerging nanodevices for refinement precision liquid biopsy techniques.

Language: Английский

Citations

10
Engineering Cell Membranes: From Extraction Strategies to Emerging Biosensing Applications DOI

Jing Tan,

Chengcheng Zhu, Lulu Li

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(20), P. 7880 - 7894

Published: Jan. 25, 2024

ADVERTISEMENT RETURN TO ARTICLES ASAPPREVReviewNEXTEngineering Cell Membranes: From Extraction Strategies to Emerging Biosensing ApplicationsJing TanJing TanCollege of Chemistry and Materials Science, Nanjing Normal University, 210023, P.R. ChinaMore by Jing TanView Biography, Chengcheng ZhuChengcheng ZhuCollege ZhuView Lulu LiLulu LiCollege Environmental Chemical Engineering, Jiangsu University Science Technology, Zhenjiang 212000, LiView Biographyhttps://orcid.org/0000-0001-6781-1826, Jin Wang*Jin WangCollege China*[email protected]More WangView Biographyhttps://orcid.org/0000-0003-4525-9924, Xing-Hua XiaXing-Hua XiaState Key Laboratory Analytical for Life XiaView Biographyhttps://orcid.org/0000-0001-9831-4048, Chen Wang*Chen ChinaState Biographyhttps://orcid.org/0000-0001-6544-4065Cite this: Anal. Chem. 2024, XXXX, XXX, XXX-XXXPublication Date (Web):January 25, 2024Publication History Received22 April 2023Accepted4 January 2024Revised1 August 2023Published online25 2024https://doi.org/10.1021/acs.analchem.3c01746© 2024 American SocietyRequest reuse permissionsArticle Views572Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biosensing,Cells,Membranes,Nanoparticles,Plasma membrane Get e-Alerts

Language: Английский

Citations

10
The rising roles of exosomes in the tumor microenvironment reprogramming and cancer immunotherapy DOI Creative Commons

Yu Wu,

Wenyan Han,

Hairong Dong

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(4)

Published: April 1, 2024

Abstract Exosomes are indispensable for intercellular communications. Tumor microenvironment (TME) is the living environment of tumor cells, which composed various components, including immune cells. Based on TME, immunotherapy has been recently developed eradicating cancer cells by reactivating antitumor effect The communications between and TME crucial development, metastasis, drug resistance. play an important role in mediating these regulating reprogramming affects sensitivity immunotherapy. Therefore, it imperative to investigate exosomes impact Here, we review communication remodeling efficacy immunotherapy, as well summarize underlying mechanisms. Furthermore, also introduce potential application artificially modified delivery systems drugs. Further efforts this field will provide new insights roles progression, thus helping us uncover effective strategies treatment.

Language: Английский

Citations

10
The new advance of exosome-based liquid biopsy for cancer diagnosis DOI Creative Commons

Haozhou Tang,

Dan Yu, Jiahui Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 8, 2024

Liquid biopsy is a minimally invasive method that uses biofluid samples instead of tissue for cancer diagnosis. Exosomes are small extracellular vesicles secreted by donor cells and act as mediators intercellular communication in human health disease. Due to their important roles, exosomes have been considered promising biomarkers liquid biopsy. However, traditional methods exosome isolation cargo detection time-consuming inefficient, limiting practical application. In the past decades, many new strategies, such microfluidic chips, nanowire arrays electrochemical biosensors, proposed achieve rapid, accurate high-throughput analysis exosomes. this review, we discussed about advance exosome-based technology, including isolation, enrichment, approaches. The comparison currently available also included. Finally, summarized advantages limitations present strategies further gave perspective future translational use.

Language: Английский

Citations

9