Critical Reviews in Food Science and Nutrition,
Год журнала:
2024,
Номер
unknown, С. 1 - 16
Опубликована: Апрель 24, 2024
Global
food
safety
stands
out
as
a
prominent
public
concern,
affecting
populations
worldwide.
The
recurrent
challenge
of
incidents
reveals
the
need
for
robust
inspection
framework.
In
recent
years,
integration
isothermal
nucleic
acid
amplification
with
CRISPR-Cas12a
techniques
has
emerged
promising
tool
molecular
detection
hazards,
presenting
next
generation
biosensing
detection.
This
paper
provides
comprehensive
review
current
state
research
on
synergistic
application
and
technology
in
field
safety.
innovative
combination
not
only
enriches
analytical
tools,
but
also
improving
assay
performance
such
sensitivity
specificity,
addressing
limitations
traditional
methods.
summarized
various
methodologies
by
diverse
concerns,
including
pathogenic
bacterium,
viruses,
mycotoxins,
adulteration,
genetically
modified
foods.
Each
section
elucidates
specific
strategies
employed
highlights
advantages
conferred.
Furthermore,
discussed
challenges
faced
this
context
safety,
offering
insightful
discussions
potential
solutions
future
prospects.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(18), С. 7274 - 7280
Опубликована: Апрель 24, 2024
Inspired
by
natural
DNA
networks,
programmable
artificial
networks
have
become
an
attractive
tool
for
developing
high-performance
biosensors.
However,
there
is
still
a
lot
of
room
expansion
in
terms
sensitivity,
atom
economy,
and
result
self-validation
current
microRNA
sensors.
In
this
protocol,
miRNA-122
as
target
model,
ultrasensitive
fluorescence
(FL)
photoelectrochemical
(PEC)
dual-mode
biosensing
platform
developed
using
entropy-driven
circuit
(EDC)
cascaded
self-feedback
DNAzyme
network.
The
well-designed
EDC
realizes
full
utilization
the
strands
improves
atomic
economy
signal
amplification
system.
unique
rational
design
double-CdSe
quantum-dot-released
substrate
network
significantly
avoids
high
background
signals
enhances
sensitivity
specificity.
Also,
enzyme-free,
effectively
risk
leakage
accuracy
sensor.
Moreover,
introduction
superparamagnetic
Fe
Environmental Technology & Innovation,
Год журнала:
2024,
Номер
34, С. 103625 - 103625
Опубликована: Апрель 4, 2024
Contaminants,
such
as
nucleic
acids
or
toxic
small
molecules,
threaten
both
human
health
and
ecosystems
when
they
infiltrate
the
environment.
The
precise
highly
sensitive
identification
of
contaminants
holds
paramount
importance
across
diverse
domains,
including
safeguarding
food
integrity,
facilitating
clinical
diagnostics,
monitoring
environmental
conditions.
Traditional
methodologies,
encompassing
spectroscopy,
chromatography,
sequencing,
metagenomics,
have
conventionally
served
pivotal
roles
in
detection
processes.
Nevertheless,
these
methods
encountered
recurring
challenges
related
to
sensitivity,
specificity,
portability.
This
review
focuses
on
groundbreaking
CRISPR/Cas12-based
biosensors.
These
biosensors
leverage
incredible
precision
programmability
CRISPR/Cas
system
recognize
specific
targets.
Here,
we
comprehensively
assess
fundamental
mechanisms
that
enable
detection,
ranging
from
guide
RNA
design
collateral
cleavage.
versatility
CRISPR/Cas12
becomes
evident
through
their
applications.
applications
encompass
medical
safety,
monitoring.
transition
conventional
ultimately
represents
a
significant
milestone
contaminant
detection.
By
incorporating
molecular
biology,
nanotechnology,
bioinformatics,
potential
reshape
landscape
water
CRIPSR-Cas
diagnostics
is
transformative
technology
paves
way
for
safer
healthier
future
environment
life.
TrAC Trends in Analytical Chemistry,
Год журнала:
2024,
Номер
172, С. 117594 - 117594
Опубликована: Фев. 8, 2024
The
precision
and
versatility
of
CRISPR-based
techniques,
combined
with
the
advantages
nucleic
acid-based
nanotechnology,
hold
great
promise
in
transforming
landscape
molecular
diagnostics.
While
significant
progress
has
been
made,
current
platforms
primarly
focus
on
acid
detection.
To
expand
applicability
fully
leverage
offered
by
diagnostics,
ongoing
efforts
explore
strategies
to
develop
CRISPR
sensors
capable
detecting
a
diverse
range
analytes
beyond
acids.
In
addition,
challenges
still
persist
adaptation
for
point-of-care
(POC)
applications,
involving
concerns
such
as
portability
automation,
well
complexities
associated
multiplexing.
Here,
we
provide
detailed
classification
comprehensive
discussion
facilitating
conversion
non-nucleic
target
binding
into
CRISPR-powered
outputs
an
emphasis
their
corresponding
design
principles.
Furthermore,
second
part
review
outlines
potential
solutions
seamlessly
integrating
these
user-friendly
rapid
tests
specifically
tailored
(POC).
Journal of Agricultural and Food Chemistry,
Год журнала:
2023,
Номер
71(37), С. 13577 - 13594
Опубликована: Сен. 1, 2023
Non-nucleic
acid
targets
have
posed
a
serious
challenge
to
food
safety.
The
detection
of
non-nucleic
can
enable
us
monitor
contamination
in
timely
manner.
In
recent
years,
the
CRISPR/Cas
system
has
been
extensively
explored
biosensing.
However,
there
is
lack
summary
CRISPR/Cas-powered
tailored
involved
This
review
comprehensively
summarizes
advances
on
construction
and
promising
applications
field
safety
related
targets.
current
challenges
futuristic
perspectives
are
also
proposed
accordingly.
rapidly
evolving
provided
powerful
propellant
for
target
via
integration
with
aptamer
and/or
DNAzyme.
Compared
traditional
analytical
methods,
conceptually
novel,
essentially
eliminates
dependence
large
instruments,
demonstrates
capability
rapid,
accurate,
sensitive,
on-site
testing.
Food Frontiers,
Год журнала:
2023,
Номер
4(4), С. 2070 - 2080
Опубликована: Июнь 27, 2023
Abstract
It
is
imperative
to
develop
practicable
pathogenic
bacteria
detection
methods.
We
devised
a
biosensor
for
the
ultrasensitive
of
Salmonella
typhimurium
(
S.
typhi
),
termed
as
SCOUT‐dCas9
(ultrasensitive,
cross‐validating,
on‐site,
and
dUal‐mode
test
using
CRISPR/dCas9).
Simply,
species‐specific
invA
gene
was
amplified
loop‐mediated
isothermal
amplification
with
biotinylated
primer,
which
can
be
specifically
“pulled
down”
by
“antibody‐like”
dCas9‐single
guide
RNA
form
ternary
complex.
SYBR
Green
I
streptavidin‐modified
alkaline
phosphatase
were
used
functionalize
them
generate
fluorescent
colorimetric
signals,
respectively.
With
this
strategy,
target
could
dexterously
converted
into
bimodal
signals
that
cross‐validated
afford
more
reliable
results.
For
both
modes,
able
detect
low
1
CFU/mL
dynamic
range
from
10
9
CFU/mL.
Lastly,
had
satisfactory
selectivity
capable
detecting
‐contaminated
real
food
samples.
provides
robust
platform
bacterial
detection.
