Multifunctional Ethyl Violet@NH2-MIL-88B(Fe) Hybrids: CRISPR-Cas12a-Assisted PEC-FL-CL Triple-Mode Sensitive Detection of HPV-16 DOI

Hejie Zheng,

Huan Wang,

Cuicui Du

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(39), С. 15657 - 15664

Опубликована: Сен. 19, 2024

The multimode assay based on multiple response mechanisms has received great attention to effectively improve the accuracy of a sensing platform. However, multifunctional materials for simultaneously satisfying multiple-mode detections are still in shortage due incompatibility signal transduction different modes. Here, taking human papillomavirus 16 (HPV-16) DNA (TDNA) as model its important role cervical cancer, novel material, ethyl violet (EV)@NH

Язык: Английский

Applications of nanomaterial technology in biosensing DOI Creative Commons

Yaoxin Fu,

Tiegen Liu, Haonan Wang

и другие.

Journal of Science Advanced Materials and Devices, Год журнала: 2024, Номер 9(2), С. 100694 - 100694

Опубликована: Март 2, 2024

Nanomaterial technology is a comprehensive subject with strong intersections, and its related research content involves wide range of modern scientific technological fields. The science in the area nanomaterials has attracted attention many groups over past few years. By very nature, this topic lot room for research, to tiny objects nanometer range. Nanomaterials refer sudden changes properties substances when they reach scale, resulting special properties. In paper, we introduce various commonly used field biosensing, briefly explain advantages disadvantages nanoscale biosensors. At same time, also working principles types biosensors based on nanomaterial technology, including electrochemical biosensors, optical piezoelectric addition, sensing targets common such as enzymes, DNA, microorganisms, etc. Finally, discuss challenges prospects application analyze current trends future directions.

Язык: Английский

Процитировано

51

Multi‐Mode/Signal Biosensors: Electrochemical Integrated Sensing Techniques DOI
Qingzhi Han, Haimei Wang, Haimei Wang

и другие.

Advanced Functional Materials, Год журнала: 2024, Номер unknown

Опубликована: Май 14, 2024

Abstract Electrochemical (EC) analysis has emerged as a high‐sensitivity, reliable, cost‐effective, and rapidly evolving technique that garnered significant attention across diverse domains. Furthermore, EC‐based techniques hold great potential for miniaturization integration. The integration of EC with mode/signal (such light, magnetic, thermal signals, etc.) provides unique opportunities biosensors to acquire more information through single sensing platform. By coupling multiple signals or processing them logically, the detection accuracy can be further improved, probability false positives negatives minimized. In this review, thorough multi‐ sensors in field is conducted, along their various (e.g., fluorescence, photothermal, colorimetry, microfluidic, etc.). aim delve into latest advances, applications, well challenges multi‐mode/signal biosensors, where utilization modalities helps enhance accuracy, sensitivity, selectivity. This review new insight synergistic effects integrating other techniques, aiming shed light on near‐future developments EC‐integrated biosensors.

Язык: Английский

Процитировано

35

A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay DOI Creative Commons
Yan Liu, Zhujun Chao, Wei Ding

и другие.

Cellular & Molecular Biology Letters, Год журнала: 2024, Номер 29(1)

Опубликована: Март 8, 2024

Abstract Persistent infection with high-risk human papillomavirus (HR-HPV) is the primary and initiating factor for cervical cancer. With over 200 identified HPV types, including 14 types that integrate into host epithelial cell DNA, early determination of type crucial effective risk stratification management. Presently, on-site immediate testing during screening stage, known as Point Care Testing (POCT), remains immature, severely limiting scope scenarios screening. This study, guided by genomic sequence patterns HPV, established a multiplex recombinase polymerase amplification (RPA) technology based on concept “universal primers.” approach achieved multiple RPA, coupled CRISPR/Cas12a system serving medium signal conversion. The study successfully constructed POCT combined detection system, denoted H-MRC12a (HPV—Multiple RPA—CRISPR/Cas12a), applied it to typing detection. accomplished six (16, 18, 31, 33, 35, 45) can be completed within 40 min, entire process, from sample loading result interpretation, 45 depth reaching 1 copy/μL each type. Validation system’s reproducibility specificity was further conducted through QPCR 34 clinical samples. Additionally, this explored optimized RPA CRISPR at molecular mechanism level. Furthermore, primer design strategy developed in offers potential enhance throughput while ensuring sensitivity, providing novel research avenue high-throughput Point-of-Care pathogen studies.

Язык: Английский

Процитировано

16

DNA Tetrahedra as Functional Nanostructures: From Basic Principles to Applications DOI Creative Commons
Yu Ouyang, Pu Zhang, Itamar Willner

и другие.

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(41)

Опубликована: Июль 22, 2024

Abstract Self‐assembled supramolecular DNA tetrahedra composed of programmed sequence‐engineered complementary base‐paired strands represent elusive nanostructures having key contributions to the development and diverse applications nanotechnology. By appropriate engineering strands, tuneable sizes chemical functionalities were designed. Programmed for integrated into structures including sequence‐specific recognition (aptamers), catalytic DNAzymes, nanoparticles, proteins, or fluorophore. The article presents a comprehensive review addressing methods assemble characterize nanostructures, framework are discussed. Topics being addressed include application structurally functionalized nanostructure assembly optical electrochemical sensing platforms intracellular imaging modules. In addition, triggered reconfiguration dynamic networks circuits emulating biological transformations introduced. Moreover, functionalization frameworks with nanoparticles provides building units devices crystallization nanoparticle superlattices. Finally, in field nanomedicine addressed. These tetrahedra‐assisted permeation nanocarriers cells imaging, controlled drug release, active chemodynamic/photodynamic treatment target tissues, regenerative medicine.

Язык: Английский

Процитировано

14

CRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious Diseases DOI

Yupiao Xia,

Ruotong Rao,

Mengqiu Xiong

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(20), С. 8091 - 8108

Опубликована: Март 7, 2024

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTCRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious DiseasesYupiao XiaYupiao XiaState Key Laboratory Magnetic Resonance and Atomic Molecular Physics, in Biological Systems, National Center Wuhan, Wuhan Institute Physics Mathematics, Innovation Academy Precision Measurement Science Technology, Chinese Sciences − Optoelectronics, 430071, ChinaUniversity Sciences, Beijing 100049, ChinaMore by Yupiao XiaView Biography, Ruotong RaoRuotong RaoState RaoView Mengqiu XiongMengqiu XiongState ChinaDepartment Medicine, Nanjing First Hospital, Medical University, 210006, XiongView Bangshun He*Bangshun HeDepartment China*[email protected]More HeView Bingxin ZhengBingxin ZhengState ZhengView Yanwei JiaYanwei JiaState-Key Analog Mixed-Signal VLSI, Microelectronics, University Macau, Macau 999078, JiaView Biographyhttps://orcid.org/0000-0001-5795-7450, Ying Li*Ying LiState LiView Biographyhttps://orcid.org/0000-0001-8689-5578, Yunhuang Yang*Yunhuang YangState ChinaOptics Valley Laboratory, Hubei 430074, YangView BiographyCite this: Anal. Chem. 2024, 96, 20, 8091–8108Publication Date (Web):March 7, 2024Publication History Received27 September 2023Accepted23 February 2024Revised7 2024Published online7 March inissue 21 May 2024https://pubs.acs.org/doi/10.1021/acs.analchem.3c04363https://doi.org/10.1021/acs.analchem.3c04363review-articleACS PublicationsCopyright © 2024 American Chemical SocietyRequest reuse permissionsArticle Views1329Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biotechnology,Fluorescence,Genetics,Nucleic acids,SARS-CoV-2 Get e-Alerts

Язык: Английский

Процитировано

13

Enhancing 3D DNA Walker-Induced CRISPR/Cas12a Technology for Highly Sensitive Detection of ExomicroRNA Associated with Osteoporosis DOI
Lijuan Luo, Fang Dong, Dandan Li

и другие.

ACS Sensors, Год журнала: 2024, Номер 9(3), С. 1438 - 1446

Опубликована: Март 7, 2024

Exosomal microRNAs (exomiRNAs) have emerged as promising biomarkers for the early clinical diagnosis of osteoporosis. However, their limited abundance and short length in peripheral blood present significant challenges accurate detection exomiRNAs. Herein, we designed implemented an efficacious fluorescence-based biosensor highly sensitive exomiRNA associated with osteoporosis, leveraging enhancing 3D DNA walker-induced CRISPR/Cas12a technology. The engineered walker is capable efficiently transforming target into amplifying strands, thereby sensitivity developed biosensor. Concurrently, liberated strands serve activators to trigger Cas12a trans-cleavage activity, culminating a significantly amplified fluorescent signal exomiRNA-214. Under optimal conditions, devised technology demonstrated capacity detect exomiRNA-214 at concentrations low 20.42 fM, encompassing wide linear range extending from 50.0 fM 10.0 nM. Moreover, could accurately differentiate between healthy individuals osteoporosis patients via exomiRNA-214, which was agreement RT-qPCR results. As such, this biosensing offers promise valuable tool

Язык: Английский

Процитировано

10

A modularized universal strategy by integrating CRISPR/Cas with nanoporous materials for ultrasensitive determination of nucleic acids DOI

Hongkai Wang,

Yinzheng Niu, Hui Liu

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160065 - 160065

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

2

CRISPR integrated biosensors: A new paradigm for cancer detection DOI

Ashok Saini,

Neeraj Dilbaghi, Neelam Yadav

и другие.

Clinica Chimica Acta, Год журнала: 2025, Номер 569, С. 120179 - 120179

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

2

Dual-OR Logic-Gated Lateral Flow Strip Assay Based on Colorimetric-Fluorescence Dual Indication for Screening of HPV16/18 in Multiple Scenarios DOI
Jiwei Wang, Shuo Ma,

Kezhen Ge

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 29, 2025

The incidence of cervical cancer continues to rise in underdeveloped regions due low human papillomavirus (HPV) vaccination rates and inadequate screening systems. To achieve convenient, rapid, accurate detection HPV, we developed a three-wire lateral flow strip assay system based on dual-OR logic gates for rapid simultaneous HPV subtypes 16 18 single test. combines three-branch-catalytic hairpin assembly (TCHA)-mediated signal amplification with simple OR gate-based output improve while enabling 16/18 subtype identification. limit the method was calculated be 10 aM selected target sequences. Meanwhile, showed excellent specificity no false-positive real-world detection. sensitivity colorimetric test strips exceeded 90%, fluorescence-based surpassed 95% detecting clinical samples, demonstrating high degree concordance results obtained from real-time quantitative polymerase chain reaction (qPCR). This provides easy HPV.

Язык: Английский

Процитировано

1

Application of CRISPR-Cas System in HPV Detection Using Biosensor Devices and POC Technologies DOI Creative Commons
Chang He, Yongqi Li,

Jinkuan Liu

и другие.

BME Frontiers, Год журнала: 2025, Номер 6

Опубликована: Янв. 1, 2025

Human papillomavirus (HPV) is the most common virus for genital tract infections. Cervical cancer ranks as fourth prevalent globally, with over 99% of cases in women attributed to HPV infection. This infection continues pose an ongoing threat public health. Therefore, development rapid, high-throughput, and sensitive detection platforms important, especially regions limited access advanced medical resources. CRISPR-based biosensors, a promising new method nucleic acid detection, are now rapidly widely used basic applied research have received much attention recent years diagnosis treatment. In this review, we discuss mechanisms functions CRISPR-Cas system, focusing on its applications diagnostics. The review covers CRISPR technologies such CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, along amplification methods, signal output systems, point-of-care testing (POCT) strategies. comprehensive overview highlights versatility potential detection. We also numerous biosensors developed since introduction detect HPV. Finally, some challenges faced by system.

Язык: Английский

Процитировано

1