Nanomaterials-Integrated CRISPR/Cas Systems: Expanding the Toolbox for Optical Detection DOI
Tianying Sun, Weiwei He, Xiangmei Chen

et al.

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Nanomaterials-integrated CRISPR/Cas systems have rapidly emerged as powerful next-generation platforms for optical biosensing. These integrated harness the precision of CRISPR/Cas-mediated nucleic acid detection while leveraging unique properties nanomaterials to achieve enhanced sensitivity and expanded analytical capabilities, thereby broadening their diagnostic potential. By incorporating a diverse range nanomaterials, these effectively expand toolbox detection, offering adaptable solutions tailored various challenges. This review provides comprehensive overview successfully into CRISPR/Cas-based sensing systems. It examines multiple modalities, including fluorescence, electrochemiluminescence, colorimetry, surface-enhanced Raman spectroscopy, highlighting how facilitate signal amplification, enable multiplexing, support development point-of-care applications. Additionally, practical applications in critical fields such healthcare diagnostics environmental monitoring are showcased. While offer considerable advantages, several real-world challenges complexity assay workflows, impact cost, regulatory hurdles must be addressed before widespread implementation can achieved. identifying obstacles proposing strategic solutions, we aim pave way continued advancement adoption nanomaterial-integrated biosensing technologies.

Language: Английский

Applications of nanomaterial technology in biosensing DOI Creative Commons

Yaoxin Fu,

Tiegen Liu, Haonan Wang

et al.

Journal of Science Advanced Materials and Devices, Journal Year: 2024, Volume and Issue: 9(2), P. 100694 - 100694

Published: March 2, 2024

Nanomaterial technology is a comprehensive subject with strong intersections, and its related research content involves wide range of modern scientific technological fields. The science in the area nanomaterials has attracted attention many groups over past few years. By very nature, this topic lot room for research, to tiny objects nanometer range. Nanomaterials refer sudden changes properties substances when they reach scale, resulting special properties. In paper, we introduce various commonly used field biosensing, briefly explain advantages disadvantages nanoscale biosensors. At same time, also working principles types biosensors based on nanomaterial technology, including electrochemical biosensors, optical piezoelectric addition, sensing targets common such as enzymes, DNA, microorganisms, etc. Finally, discuss challenges prospects application analyze current trends future directions.

Language: Английский

Citations

51
Multi‐Mode/Signal Biosensors: Electrochemical Integrated Sensing Techniques DOI
Qingzhi Han, Haimei Wang, Haimei Wang

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: May 14, 2024

Abstract Electrochemical (EC) analysis has emerged as a high‐sensitivity, reliable, cost‐effective, and rapidly evolving technique that garnered significant attention across diverse domains. Furthermore, EC‐based techniques hold great potential for miniaturization integration. The integration of EC with mode/signal (such light, magnetic, thermal signals, etc.) provides unique opportunities biosensors to acquire more information through single sensing platform. By coupling multiple signals or processing them logically, the detection accuracy can be further improved, probability false positives negatives minimized. In this review, thorough multi‐ sensors in field is conducted, along their various (e.g., fluorescence, photothermal, colorimetry, microfluidic, etc.). aim delve into latest advances, applications, well challenges multi‐mode/signal biosensors, where utilization modalities helps enhance accuracy, sensitivity, selectivity. This review new insight synergistic effects integrating other techniques, aiming shed light on near‐future developments EC‐integrated biosensors.

Language: Английский

Citations

32
A multiplex RPA-CRISPR/Cas12a-based POCT technique and its application in human papillomavirus (HPV) typing assay DOI Creative Commons
Yan Liu, Zhujun Chao, Wei Ding

et al.

Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)

Published: March 8, 2024

Abstract Persistent infection with high-risk human papillomavirus (HR-HPV) is the primary and initiating factor for cervical cancer. With over 200 identified HPV types, including 14 types that integrate into host epithelial cell DNA, early determination of type crucial effective risk stratification management. Presently, on-site immediate testing during screening stage, known as Point Care Testing (POCT), remains immature, severely limiting scope scenarios screening. This study, guided by genomic sequence patterns HPV, established a multiplex recombinase polymerase amplification (RPA) technology based on concept “universal primers.” approach achieved multiple RPA, coupled CRISPR/Cas12a system serving medium signal conversion. The study successfully constructed POCT combined detection system, denoted H-MRC12a (HPV—Multiple RPA—CRISPR/Cas12a), applied it to typing detection. accomplished six (16, 18, 31, 33, 35, 45) can be completed within 40 min, entire process, from sample loading result interpretation, 45 depth reaching 1 copy/μL each type. Validation system’s reproducibility specificity was further conducted through QPCR 34 clinical samples. Additionally, this explored optimized RPA CRISPR at molecular mechanism level. Furthermore, primer design strategy developed in offers potential enhance throughput while ensuring sensitivity, providing novel research avenue high-throughput Point-of-Care pathogen studies.

Language: Английский

Citations

16
CRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious Diseases DOI

Yupiao Xia,

Ruotong Rao,

Mengqiu Xiong

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(20), P. 8091 - 8108

Published: March 7, 2024

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTCRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious DiseasesYupiao XiaYupiao XiaState Key Laboratory Magnetic Resonance and Atomic Molecular Physics, in Biological Systems, National Center Wuhan, Wuhan Institute Physics Mathematics, Innovation Academy Precision Measurement Science Technology, Chinese Sciences − Optoelectronics, 430071, ChinaUniversity Sciences, Beijing 100049, ChinaMore by Yupiao XiaView Biography, Ruotong RaoRuotong RaoState RaoView Mengqiu XiongMengqiu XiongState ChinaDepartment Medicine, Nanjing First Hospital, Medical University, 210006, XiongView Bangshun He*Bangshun HeDepartment China*[email protected]More HeView Bingxin ZhengBingxin ZhengState ZhengView Yanwei JiaYanwei JiaState-Key Analog Mixed-Signal VLSI, Microelectronics, University Macau, Macau 999078, JiaView Biographyhttps://orcid.org/0000-0001-5795-7450, Ying Li*Ying LiState LiView Biographyhttps://orcid.org/0000-0001-8689-5578, Yunhuang Yang*Yunhuang YangState ChinaOptics Valley Laboratory, Hubei 430074, YangView BiographyCite this: Anal. Chem. 2024, 96, 20, 8091–8108Publication Date (Web):March 7, 2024Publication History Received27 September 2023Accepted23 February 2024Revised7 2024Published online7 March inissue 21 May 2024https://pubs.acs.org/doi/10.1021/acs.analchem.3c04363https://doi.org/10.1021/acs.analchem.3c04363review-articleACS PublicationsCopyright © 2024 American Chemical SocietyRequest reuse permissionsArticle Views1329Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biotechnology,Fluorescence,Genetics,Nucleic acids,SARS-CoV-2 Get e-Alerts

Language: Английский

Citations

13
DNA Tetrahedra as Functional Nanostructures: From Basic Principles to Applications DOI Creative Commons
Yu Ouyang, Pu Zhang, Itamar Willner

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(41)

Published: July 22, 2024

Abstract Self‐assembled supramolecular DNA tetrahedra composed of programmed sequence‐engineered complementary base‐paired strands represent elusive nanostructures having key contributions to the development and diverse applications nanotechnology. By appropriate engineering strands, tuneable sizes chemical functionalities were designed. Programmed for integrated into structures including sequence‐specific recognition (aptamers), catalytic DNAzymes, nanoparticles, proteins, or fluorophore. The article presents a comprehensive review addressing methods assemble characterize nanostructures, framework are discussed. Topics being addressed include application structurally functionalized nanostructure assembly optical electrochemical sensing platforms intracellular imaging modules. In addition, triggered reconfiguration dynamic networks circuits emulating biological transformations introduced. Moreover, functionalization frameworks with nanoparticles provides building units devices crystallization nanoparticle superlattices. Finally, in field nanomedicine addressed. These tetrahedra‐assisted permeation nanocarriers cells imaging, controlled drug release, active chemodynamic/photodynamic treatment target tissues, regenerative medicine.

Language: Английский

Citations

13
Enhancing 3D DNA Walker-Induced CRISPR/Cas12a Technology for Highly Sensitive Detection of ExomicroRNA Associated with Osteoporosis DOI
Lijuan Luo, Fang Dong, Dandan Li

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(3), P. 1438 - 1446

Published: March 7, 2024

Exosomal microRNAs (exomiRNAs) have emerged as promising biomarkers for the early clinical diagnosis of osteoporosis. However, their limited abundance and short length in peripheral blood present significant challenges accurate detection exomiRNAs. Herein, we designed implemented an efficacious fluorescence-based biosensor highly sensitive exomiRNA associated with osteoporosis, leveraging enhancing 3D DNA walker-induced CRISPR/Cas12a technology. The engineered walker is capable efficiently transforming target into amplifying strands, thereby sensitivity developed biosensor. Concurrently, liberated strands serve activators to trigger Cas12a trans-cleavage activity, culminating a significantly amplified fluorescent signal exomiRNA-214. Under optimal conditions, devised technology demonstrated capacity detect exomiRNA-214 at concentrations low 20.42 fM, encompassing wide linear range extending from 50.0 fM 10.0 nM. Moreover, could accurately differentiate between healthy individuals osteoporosis patients via exomiRNA-214, which was agreement RT-qPCR results. As such, this biosensing offers promise valuable tool

Language: Английский

Citations

10
A modularized universal strategy by integrating CRISPR/Cas with nanoporous materials for ultrasensitive determination of nucleic acids DOI

Hongkai Wang,

Yinzheng Niu, Hui Liu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160065 - 160065

Published: Jan. 1, 2025

Language: Английский

Citations

1
Dual-OR Logic-Gated Lateral Flow Strip Assay Based on Colorimetric-Fluorescence Dual Indication for Screening of HPV16/18 in Multiple Scenarios DOI
Jiwei Wang, Shuo Ma,

Kezhen Ge

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

The incidence of cervical cancer continues to rise in underdeveloped regions due low human papillomavirus (HPV) vaccination rates and inadequate screening systems. To achieve convenient, rapid, accurate detection HPV, we developed a three-wire lateral flow strip assay system based on dual-OR logic gates for rapid simultaneous HPV subtypes 16 18 single test. combines three-branch-catalytic hairpin assembly (TCHA)-mediated signal amplification with simple OR gate-based output improve while enabling 16/18 subtype identification. limit the method was calculated be 10 aM selected target sequences. Meanwhile, showed excellent specificity no false-positive real-world detection. sensitivity colorimetric test strips exceeded 90%, fluorescence-based surpassed 95% detecting clinical samples, demonstrating high degree concordance results obtained from real-time quantitative polymerase chain reaction (qPCR). This provides easy HPV.

Language: Английский

Citations

1
CRISPR integrated biosensors: A new paradigm for cancer detection DOI

Ashok Saini,

Neeraj Dilbaghi, Neelam Yadav

et al.

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: 569, P. 120179 - 120179

Published: Feb. 1, 2025

Language: Английский

Citations

1
Smartphone‐Based Free‐to‐Total Prostate Specific Antigen Ratio Detection System Using a Colorimetric Reaction Integrated with Proximity‐Induced Bio‐Barcode and CRISPR/Cas12a Assay DOI
Bin Luo, Juan Zhou, Xiaohui Zhan

et al.

Small, Journal Year: 2024, Volume and Issue: 20(28)

Published: Feb. 11, 2024

Abstract The free‐to‐total prostate‐specific antigen (f/t‐PSA) ratio is of great significance in the accurate diagnosis prostate cancer. Herein, a smartphone‐based detection system reported using colorimetric reaction integrated with proximity‐induced bio‐barcode and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a assay for f/t‐PSA detection. DNA/antibody recognition probes are designed to bind f‐PSA or t‐PSA induce release DNA bio‐barcode. CRISPR/Cas12a activated by Ag+ from C‐Ag+‐C structure hairpin DNA. released used affect tetramethylbenzidine (TMB)‐H2O2‐based catalyzed Pt nanoparticles (NPs), as peroxidase‐like activity NPs can be efficiently inhibited Ag+. A smartphone self‐developed app an image reader analyzer analyze provide results. limit 0.06 0.04 ng mL −1 achieved f‐PSA, respectively. method showed linear response between 0.1 100 f‐PSA. In tests clinical samples, successfully diagnosed cancer patients benign prostatic hyperplasia healthy cases high sensitivity specificity.

Language: Английский

Citations

8