Application of bioelectrical impedance detection techniques: cells and tissues
Biosensors and Bioelectronics,
Год журнала:
2025,
Номер
273, С. 117159 - 117159
Опубликована: Янв. 11, 2025
Язык: Английский
Three-dimensional inertial focusing based impedance cytometer enabling high-accuracy characterization of electrical properties of tumor cells
Lab on a Chip,
Год журнала:
2024,
Номер
24(18), С. 4333 - 4343
Опубликована: Янв. 1, 2024
The
differences
in
the
cross-sectional
positions
of
cells
detection
area
have
a
severe
negative
impact
on
achieving
accurate
characterization
impedance
spectra
cells.
Herein,
we
proposed
three-dimensional
(3D)
inertial
focusing
based
cytometer
integrating
sheath
fluid
compression
and
for
high-accuracy
electrical
identification
tumor
First,
studied
effects
particle
initial
position
focusing.
Then,
relationship
height
signal-to-noise
ratio
(SNR)
signal
was
explored.
results
showed
that
efficient
single-line
7-20
μm
particles
close
to
electrodes
achieved
signals
with
high
SNR
low
coefficient
variation
(CV)
were
obtained.
Finally,
properties
three
types
(A549,
MDA-MB-231,
UM-UC-3
cells)
accurately
characterized.
Machine
learning
algorithms
implemented
identify
amplitude
phase
opacities
at
multiple
frequencies.
Compared
traditional
two-dimensional
(2D)
focusing,
accuracy
A549,
using
our
3D
increased
by
57.5%,
36.4%
36.6%,
respectively.
enables
wide
size
range
without
causing
clogging
obtains
signals,
improving
applicability
different
complex
biological
samples
cell
accuracy.
Язык: Английский
An Integrated Microfluidic Microwave Array Sensor with Machine Learning for Enrichment and Detection of Mixed Biological Solution
Biosensors,
Год журнала:
2025,
Номер
15(1), С. 45 - 45
Опубликована: Янв. 13, 2025
In
this
work,
an
integrated
microfluidic
microwave
array
sensor
is
proposed
for
the
enrichment
and
detection
of
mixed
biological
solution.
individuals
with
urinary
tract
infections
or
intestinal
health
issues,
levels
white
blood
cells
(WBCs)
Escherichia
coli
(E.
coli)
in
urine
extracts
can
be
significantly
elevated
compared
to
normal.
The
chip,
characterized
by
its
low
cost,
simplicity
operation,
fast
response,
high
accuracy,
designed
detect
a
solution
WBCs
E.
coli.
results
demonstrate
that
microfluidics
could
effectively
enrich
efficiency
88.3%.
For
WBC
detection,
resonance
frequency
sensing
chip
decreases
increasing
concentration,
while
capacitance
value
increases
concentration.
Furthermore,
measurement
data
are
processed
using
machine
learning.
Specifically,
subjected
further
linear
fitting.
addition,
prediction
model
employing
four
different
algorithms,
achieves
maximum
accuracy
95.24%.
Consequently,
employed
clinical
diagnosis
coli,
providing
novel
approach
medical
research
involving
bacteria.
Язык: Английский
Micropumps and Microvalves for Biomedical Applications
TrAC Trends in Analytical Chemistry,
Год журнала:
2025,
Номер
unknown, С. 118236 - 118236
Опубликована: Март 1, 2025
Язык: Английский
Analysis of Single Nuclei in a Microfluidic Cytometer Towards Metaphase Enrichment
Electrophoresis,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 10, 2025
ABSTRACT
Identifying
analyzable
metaphase
chromosomes
is
crucial
for
karyotyping,
a
common
procedure
used
by
clinicians
to
diagnose
genetic
disorders
and
some
forms
of
cancer.
This
task
often
laborious
time‐consuming,
making
it
essential
develop
automated,
efficient,
reliable
methods
assist
clinical
technicians.
In
this
work,
an
original
label‐free
microfluidic
approach
identify
potential
metaphases
developed
that
uses
impedance‐based
detection
individual
flowing
nuclei
machine‐learning‐based
processing
synchronized
high‐speed
videos.
Specifically,
impedance
signals
are
nucleus‐containing
frames,
which
then
processed
extract
the
contour
each
nucleus.
Feature
extraction
performed,
both
unsupervised
supervised
classification
approaches
implemented
from
those
features.
The
proposed
framework
tested
on
K562
cells,
highest
accuracy
obtained
with
coupled
feature
selection
Synthetic
Minority
Over‐sampling
Technique
(SMOTE).
Overall,
study
encourages
future
developments
aimed
at
integrating
sorting
functionality
in
device,
thus
achieving
effective
system
enrichment.
Язык: Английский
Recent Advances in Microfluidic Impedance Detection: Principle, Design and Applications
Micromachines,
Год журнала:
2025,
Номер
16(6), С. 683 - 683
Опубликована: Июнь 5, 2025
Under
the
dual
drivers
of
precision
medicine
development
and
health
monitoring
demands,
real-time
biosensing
technologies
has
emerged
as
a
key
breakthrough
in
field
life
science
analytics.
Microfluidic
impedance
detection
technology,
achieved
through
integration
microscale
fluid
manipulation
bioimpedance
spectrum
analysis,
enabled
biological
samples
ranging
from
single
cells
to
organ-level
systems,
now
standing
at
forefront
research.
This
review
systematically
summarizes
core
principles
microfluidic
modeling
methods
for
cell
equivalent
circuits,
system
optimization
strategies,
recent
research
advancements
applications.
We
first
elucidate
fundamental
technologies,
followed
by
comprehensive
analysis
cellular
circuit
model
construction
design
strategies.
Subsequently,
we
categorize
applications
based
on
sample
types,
elaborating
respective
progress
existing
challenges.
concludes
with
prospective
insights
into
future
developmental
trajectories.
hope
this
work
will
provide
novel
perspectives
advancing
technology
while
stimulating
interdisciplinary
collaboration
among
researchers
biology,
medicine,
chemistry,
physics
propel
technological
innovation
collectively.
Язык: Английский
Label‐Free Impedance Analysis of Induced Pluripotent Stem Cell‐Derived Spinal Cord Progenitor Cells for Rapid Safety and Efficacy Profiling
Advanced Materials Technologies,
Год журнала:
2024,
Номер
9(20)
Опубликована: Июль 5, 2024
Abstract
Regenerative
therapies,
including
the
transplantation
of
spinal
cord
progenitor
cells
(SCPCs)
derived
from
induced
pluripotent
stem
(iPSCs),
are
promising
treatment
strategies
for
injuries.
However,
risk
tumorigenicity
residual
iPSCs
advocates
an
unmet
need
rapid
SCPCs
safety
profiling.
Herein,
a
(≈3000
min
‐1
)
electrical‐based
microfluidic
biophysical
cytometer
is
reported
to
detect
low‐abundance
at
single‐cell
resolution.
Based
on
multifrequency
impedance
measurements
(0.3
12
MHz),
features
cell
size,
deformability,
membrane,
and
nucleus
dielectric
properties
simultaneously
quantified
as
hydrodynamically
stretched
cross
junction
under
continuous
flow.
A
supervised
uniform
manifold
approximation
projection
(UMAP)
model
further
developed
impedance‐based
quantification
undifferentiated
with
high
sensitivity
(≈1%
spiked
iPSCs)
shows
good
correlations
differentiation
outcomes
using
two
iPSC
lines.
Cell
membrane
opacity
(day
1)
also
identified
novel
early
intrinsic
predictive
biomarker
that
exhibits
strong
correlation
SCPC
efficiency
10).
Overall,
it
envisioned
this
label‐free
optic‐free
platform
technology
can
be
versatile
cost‐effective
process
analytical
tool
monitor
or
assess
quality
in
regenerative
medicine.
Язык: Английский
Label-free high-throughput impedance-activated cell sorting
Lab on a Chip,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
A
label-free
high-throughput
impedance-activated
cell
sorting
platform
can
sort
cells
at
a
throughput
of
1000
events
per
s.
Язык: Английский
Study on inertial migration behaviours under secondary flow patterns using a fluid-structure interaction approach
Journal of Physics Conference Series,
Год журнала:
2024,
Номер
2808(1), С. 012090 - 012090
Опубликована: Июль 1, 2024
Abstract
In
the
present
work,
critical
aspects
of
how
particles
influence
structure
secondary
flows
are
explored.
Despite
its
significance
in
manipulation
and
detection
cells
microfluidic
applications,
this
topic
has
received
limited
attention
until
now.
Employing
a
fluid-structure
interaction
method,
research
examines
two-way
coupling
between
at
Reynolds
number
(Re)
80,
revealing
significant
insights
into
behaviour
under
such
conditions.
The
investigation
explores
intricate
relationships
particle
transverse
migration,
rotation,
flow
fields,
emphasizing
these
interactions
amplify
with
both
size
intensity.
findings
underscore
role
vortices
generating
drag
forces,
which
turn
motion.
Notably,
study
demonstrates
that
for
larger
(
/H=0.375),
vortex-induced
additional
force
acts
repulsively,
discouraging
their
movement
along
flow.
Through
detailed
analysis,
paper
elucidates
lateral
migration
particles,
rotational
dynamics
response
to
flows,
overarching
effects
structures.
This
provides
fundamental
mechanisms
governing
environments,
offering
potential
advancements
detection.
Язык: Английский