Chemical Reviews,
Год журнала:
2021,
Номер
121(8), С. 5042 - 5092
Опубликована: Апрель 1, 2021
Polymer
networks
are
complex
systems
consisting
of
molecular
components.
Whereas
the
properties
individual
components
typically
well
understood
by
most
chemists,
translating
that
chemical
insight
into
polymer
themselves
is
limited
statistical
and
poorly
defined
nature
network
structures.
As
a
result,
it
challenging,
if
not
currently
impossible,
to
extrapolate
from
behavior
full
range
performance
entire
network.
therefore
present
an
unrealized,
important,
interdisciplinary
opportunity
exert
molecular-level,
control
on
material
macroscopic
properties.
A
barrier
sophisticated
approaches
techniques
for
characterizing
structure
often
unfamiliar
many
scientists.
Here,
we
critical
overview
current
characterization
available
understand
relation
between
resulting
networks,
in
absence
added
fillers.
We
highlight
methods
characterize
chemistry
molecular-level
strands
junctions,
gelation
process
which
form
network,
dynamics
mechanics
final
material.
The
purpose
serve
as
detailed
manual
conducting
these
measurements
but
rather
unify
underlying
principles,
point
out
remaining
challenges,
provide
concise
chemists
can
plan
strategies
suit
their
research
objectives.
Because
cannot
be
sufficiently
characterized
with
single
method,
strategic
combinations
multiple
required
characterization.
Annual Review of Biophysics,
Год журнала:
2022,
Номер
51(1), С. 301 - 326
Опубликована: Фев. 4, 2022
Super-resolution
microscopy
techniques,
and
specifically
single-molecule
localization
(SMLM),
are
approaching
nanometer
resolution
inside
cells
thus
have
great
potential
to
complement
structural
biology
techniques
such
as
electron
for
cell
biology.
In
this
review,
we
introduce
the
different
flavors
of
super-resolution
microscopy,
with
a
special
emphasis
on
SMLM
MINFLUX
(minimal
photon
flux).
We
summarize
recent
technical
developments
that
pushed
these
localization-based
scales
review
experimental
conditions
key
obtaining
data
highest
quality.
Furthermore,
give
an
overview
analysis
methods
highlight
studies
used
gain
insights
into
biologically
relevant
molecular
machines.
Ultimately,
our
perspective
what
is
needed
push
even
further
apply
them
investigating
dynamic
rearrangements
in
living
cells.
Chemical Society Reviews,
Год журнала:
2023,
Номер
52(20), С. 7197 - 7261
Опубликована: Янв. 1, 2023
In
the
realm
of
biological
research,
invention
super-resolution
microscopy
(SRM)
has
enabled
visualization
ultrafine
sub-cellular
structures
and
their
functions
in
live
cells
at
nano-scale
level,
beyond
diffraction
limit,
which
opened
up
a
new
window
for
advanced
biomedical
studies
to
unravel
complex
unknown
details
physiological
disorders
level
with
unprecedented
resolution
clarity.
However,
most
SRM
techniques
are
highly
reliant
on
personalized
special
photophysical
features
fluorophores.
recent
times,
there
been
an
surge
development
robust
fluorophore
systems
various
imaging
techniques.
To
date,
xanthene,
cyanine,
oxazine
BODIPY
cores
have
authoritatively
utilized
as
basic
units
small-molecule-based
organic
fluorescent
probe
designing
strategies
owing
excellent
characteristics
easy
synthetic
acquiescence.
Since
future
next-generation
will
be
decided
by
availability
probes
these
four
building
blocks
play
important
role
progressive
design,
is
urgent
need
review
advancements
fluorophores
different
methods
based
dye
cores.
This
article
not
only
includes
comprehensive
discussion
about
developments
emphasis
effective
integration
into
cell
bio-imaging
applications
but
also
critically
evaluates
background
each
highlight
merits
demerits
towards
developing
newer
SRM.
Chemistry of Materials,
Год журнала:
2023,
Номер
35(6), С. 2472 - 2485
Опубликована: Фев. 24, 2023
Stimulated
emission
depletion
(STED)
nanoscopy
has
broadened
our
horizons
to
unravel
mysterious
functions
of
cellular
structures
on
an
unparalleled
nanometer
scale.
Nevertheless,
intense
laser
power
is
a
general
prerequisite
for
STED
super-resolution
imaging
with
satisfactory
resolution,
inevitably
leading
severe
photobleaching
fluorophores,
irreparable
photodamage
biosamples,
and
impaired
quality.
Herein,
by
modulating
distinct
acceptor
units,
series
donor–acceptor–acceptor
structured
fluorescent
acrylonitriles
featured
aggregation-induced
(AIE)
at
low
were
systematically
developed.
These
AIE
luminogens
(AIEgens)
exhibited
tunable
near-infrared
emissions
(650–733
nm)
high
fluorescence
quantum
yields
(QYs
up
26.8%)
in
the
solid
state,
significant
Stokes
shifts,
large
two-photon
absorption
cross-sections.
They
also
typical
solvatochromic
effect
ultrahigh
QYs
98.4%
low-polarity
solvents.
Furthermore,
these
lipophilic
specifically
lit
lipid
droplets
(LDs)
exceptionally
photostability
wash-free
manner.
By
taking
TPA-BT-ANBI
as
example,
LDs
excellent
resolution
62
80
nm
cytosolic
nuclear
LDs,
respectively,
saturation
0.83
MW/cm2
extended
time-lapse
LD
dynamics
was
achieved.
Subsequent
use
optical
discrimination
fatty
liver
tissues
deep-tissue
demonstrated.
This
study
opens
new
avenues
versatile
photostable
materials
powers
target-specific
imaging.
Cell,
Год журнала:
2024,
Номер
187(25), С. 7045 - 7063
Опубликована: Дек. 1, 2024
Cells
are
essential
to
understanding
health
and
disease,
yet
traditional
models
fall
short
of
modeling
simulating
their
function
behavior.
Advances
in
AI
omics
offer
groundbreaking
opportunities
create
an
virtual
cell
(AIVC),
a
multi-scale,
multi-modal
large-neural-network-based
model
that
can
represent
simulate
the
behavior
molecules,
cells,
tissues
across
diverse
states.
This
Perspective
provides
vision
on
design
how
collaborative
efforts
build
AIVCs
will
transform
biological
research
by
allowing
high-fidelity
simulations,
accelerating
discoveries,
guiding
experimental
studies,
offering
new
for
cellular
functions
fostering
interdisciplinary
collaborations
open
science.
Molecules,
Год журнала:
2021,
Номер
26(1), С. 217 - 217
Опубликована: Янв. 4, 2021
Recent
advances
in
fluorescence
imaging
techniques
and
super-resolution
microscopy
have
extended
the
applications
of
fluorescent
probes
studying
various
cellular
processes
at
molecular
level.
Specifically,
organelle-targeted
been
commonly
used
to
detect
metabolites
transient
chemical
messengers
with
high
precision
become
invaluable
tools
study
biochemical
pathways.
Moreover,
several
recent
studies
reported
labeling
strategies
novel
scaffolds
enhance
target
specificity
responsiveness.
In
this
review,
we
will
survey
most
reports
assess
their
general
structural
features
on
basis
organelles.
We
discuss
advantages
currently
potential
challenges
application
as
well
future
directions.
Analytical Chemistry,
Год журнала:
2022,
Номер
94(30), С. 10676 - 10684
Опубликована: Июль 19, 2022
Lipid
droplets
(LDs)
containing
cytosolic
and
nuclear
LDs
have
recently
received
increasing
attention
because
of
their
diverse
biological
roles
in
living
systems.
However,
developing
fluorescent
probes
for
super-resolution
visualization
these
subcellular
still
remains
challenging
due
to
insufficient
fluorescence
brightness
poor
membrane
permeability.
Herein,
we
rationally
synthesized
a
series
ultrabright
solvatochromic
based
on
benzoboranils
(BBAs)
LD-specific
imaging
using
structured
illumination
microscopy
(SIM).
The
rigidly
exhibit
ultrahigh
quantum
yields
up
99.9%
low-polar
solvents.
They
also
show
more
significant
enhancements
lipid
environments
than
commercial
LD
probes.
Owing
excellent
merits,
our
lipophilic
can
specifically
light
at
ultralow
concentrations
down
10
nM.
Further
use
BBA-CF3
SIM
fusion
process
was
successfully
achieved.
unprecedented
spatial
resolution
with
an
FWHM
value
142
nm
acquired.
Collectively,
hold
tremendous
potential
unveil
the
mysterious
research
SIM.
Photobleaching
is
the
permanent
loss
of
fluorescence
after
extended
exposure
to
light
and
a
major
limiting
factor
in
super-resolution
microscopy
(SRM)
that
restricts
spatiotemporal
resolution
observation
time.
Strategies
for
preventing
or
overcoming
photobleaching
SRM
are
reviewed
developing
new
probes
chemical
environments.
Photostabilization
strategies
introduced
first,
which
borrowed
from
conventional
microscopy,
employed
SRM.
SRM-specific
then
highlighted
exploit
on-off
transitions
fluorescence,
key
mechanism
achieving
super-resolution,
becoming
routes
address
Off
states
can
serve
as
shelter
excitation
by
an
exit
release
damaged
probe
replace
it
with
fresh
one.
Such
efforts
limits
anticipated
enhance
molecular
scales
extend
time
physiological
lifespans.
