Analytical Chemistry,
Год журнала:
2021,
Номер
93(48), С. 16105 - 16112
Опубликована: Ноя. 19, 2021
Cancer
is
a
serious
threat
to
human
health,
and
there
an
urgent
need
develop
new
treatment
methods
overcome
it.
Organelle
targeting
therapy,
as
highly
effective
less
toxic
side
effect
strategy,
has
great
research
significance
development
prospects.
Being
essential
organelle,
the
Golgi
apparatus
plays
particularly
major
role
in
growth
of
cancer
cells.
Acting
indispensable
expressed
antioxidant
cells,
glutathione
(GSH)
also
contributes
greatly
during
oxidative
stress.
Therefore,
it
counts
for
much
track
changes
GSH
concentration
monitoring
occurrence
tumor
exploring
Golgi-targeted
therapy
extremely
important
cancer.
In
this
work,
we
designed
synthesized
simple
Golgi-targeting
fluorescent
probe
GT-GSH
accurately
detecting
GSH.
The
reacting
with
decomposes
substances
Golgi,
thereby
killing
At
same
time,
ratiometric
can
detect
stress
high
sensitivity
selectivity
living
such
GSH-responsive
gives
method
accurate
Chemical Reviews,
Год журнала:
2024,
Номер
124(11), С. 7106 - 7164
Опубликована: Май 17, 2024
The
identification
and
detection
of
disease-related
biomarkers
is
essential
for
early
clinical
diagnosis,
evaluating
disease
progression,
the
development
therapeutics.
Possessing
advantages
high
sensitivity
selectivity,
fluorescent
probes
have
become
effective
tools
monitoring
active
molecules
at
cellular
level
in
vivo.
In
this
review,
we
describe
current
designed
quantification
key
bioactive
associated
with
common
diseases,
such
as
organ
damage,
inflammation,
cancers,
cardiovascular
brain
disorders.
We
emphasize
strategies
behind
design
capable
biomarker
diagnosis
cover
some
aspects
combined
diagnostic/therapeutic
based
on
regulating
molecules.
This
review
concludes
a
discussion
challenges
outlook
probes,
highlighting
future
avenues
research
that
should
enable
these
to
achieve
accurate
biomedical
applications.
Abstract
Fast
and
selective
fluorescence
imaging
for
a
biomarker
to
related‐disease
diagnosis
remains
significant
challenge
due
complex
physical
environment.
Human
carboxylesterase
(CE)
is
expected
be
potential
of
hepatocellular
carcinoma
(HCC)
improve
the
accuracy
diagnosis.
However,
existing
probes
CE
has
slow
response
rate
low
selectivity.
Herein,
amide
group
selected
as
CE‐responsive
sites
based
on
“substrate‐hydrolysis
enzymatic
reaction”
approach.
From
series
off–on
with
leave
groups
in
unit,
probe
J
screened
optimal
combination
rapid
high
selectivity
toward
CE.
requires
only
150
s
reach
maximum
at
676
nm
presence
free
from
interference
other
esterase.
Computational
docking
simulations
indicate
shortest
distance
between
active
site
.
Cell
vivo
present
that
can
turn
liver
cancer
cells
tumor
region
precisely.
Importantly,
allowed
specifically
image
orthotopic
rather
than
metastatic
distinguish
human
primary
tissue
adjacent
ones.
This
study
provides
new
tool
detection
promotes
advancements
accurate
HCC
Chemical Reviews,
Год журнала:
2024,
Номер
124(20), С. 11242 - 11347
Опубликована: Окт. 9, 2024
Biopsy,
including
tissue
and
liquid
biopsy,
offers
comprehensive
real-time
physiological
pathological
information
for
disease
detection,
diagnosis,
monitoring.
Fluorescent
probes
are
frequently
selected
to
obtain
adequate
on
processes
in
a
rapid
minimally
invasive
manner
based
their
advantages
biopsy.
However,
conventional
fluorescent
have
been
found
show
aggregation-caused
quenching
(ACQ)
properties,
impeding
greater
progresses
this
area.
Since
the
discovery
of
aggregation-induced
emission
luminogen
(AIEgen)
promoted
advancements
molecular
bionanomaterials
owing
unique
high
quantum
yield
(QY)
signal-to-noise
ratio
(SNR),
Chemical Science,
Год журнала:
2020,
Номер
12(4), С. 1220 - 1226
Опубликована: Дек. 15, 2020
The
chronological
evolution
of
small
organic
molecular
fluorescent
probes
for
thiols:
from
separation
dependency
analysis
to
cellular
specific
analysis,
what's
next?
Nature Communications,
Год журнала:
2021,
Номер
12(1)
Опубликована: Июнь 23, 2021
Abstract
Intramolecular
charge
transfer
(ICT)
is
a
fundamental
mechanism
that
enables
the
development
of
numerous
fluorophores
and
probes
for
bioimaging
sensing.
However,
electron-withdrawing
targets
(EWTs)-induced
fluorescence
quenching
long-standing
unsolved
issue
in
ICT
fluorophores,
significantly
limits
widespread
applicability.
Here
we
report
simple
generalizable
structural-modification
completely
overturning
intramolecular
rotation
driving
energy,
thus
fully
reversing
fluorophores’
mode
into
light-up
mode.
Specifically,
insertion
an
indazole
unit
scaffold
can
amplify
donor-indazole-π-acceptor
(fluorescence
OFF),
whereas
efficiently
suppressing
their
EWT-substituted
system
ON).
This
molecular
strategy
generalizable,
yielding
palette
chromophores
with
umpolung
spans
visible
near-infrared
range.
expands
bio-analytical
toolboxes
allows
exploiting
sensing
EWTs
including
N
-acetyltransferases
nerve
agents.
