Complex
coacervates
are
phase-separated
liquid
droplets
composed
of
oppositely
charged
multivalent
molecules.
The
unique
material
properties
the
complex
coacervate
interior
favours
sequestration
biomolecules
and
facilitates
reactions.
Recently,
it
is
shown
that
can
be
used
for
direct
cytosolic
delivery
sequestered
in
living
cells.
Here,
studied
physical
required
oligo-arginine
RNA
to
cross
phospholipid
bilayers
enter
liposomes
penetration
depends
on
two
main
parameters:
difference
ζ-potential
between
liposomes,
partitioning
coefficient
(Kp)
lipids
into
coacervates.
Following
these
guidelines,
a
range
found
able
penetrate
membrane
cells,
thus
paving
way
further
development
as
vehicles
therapeutic
agents.
Biomolecular
condensates
present
in
cells
can
fundamentally
affect
the
aggregation
of
amyloidogenic
proteins
and
play
a
role
regulation
this
process.
While
liquid-liquid
phase
separation
by
themselves
act
as
an
alternative
nucleation
pathway,
interaction
partly
disordered
aggregation-prone
with
preexisting
that
localization
centers
could
be
far
more
general
mechanism
altering
their
behavior.
Here,
we
show
so-called
host
biomolecular
both
accelerate
slow
down
amyloid
formation.
We
study
protein
α-synuclein
two
truncated
variants
presence
three
types
composed
nonaggregating
peptides,
RNA,
or
ATP.
Our
results
demonstrate
markedly
speed
up
formation
when
localize
to
interface.
However,
also
significantly
suppress
sequestering
stabilizing
proteins,
thereby
providing
living
possible
protection
against
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Май 22, 2023
Abstract
Cells
compartmentalize
parts
of
their
interiors
into
liquid-like
condensates,
which
can
be
reconstituted
in
vitro.
Although
these
condensates
interact
with
membrane-bound
organelles,
potential
for
membrane
remodeling
and
the
underlying
mechanisms
such
interactions
are
not
well-understood.
Here,
we
demonstrate
that
between
protein
-
including
hollow
ones,
membranes
lead
to
remarkable
morphological
transformations
provide
a
theoretical
framework
describe
them.
Modulation
solution
salinity
or
composition
drives
condensate-membrane
system
through
two
wetting
transitions,
from
dewetting,
broad
regime
partial
wetting,
complete
wetting.
When
sufficient
area
is
available,
fingering
ruffling
interface
observed,
an
intriguing
phenomenon
producing
intricately
curved
structures.
The
observed
morphologies
governed
by
interplay
adhesion,
elasticity,
interfacial
tension.
Our
results
highlight
relevance
cell
biology,
pave
way
design
synthetic
membrane-droplet
based
biomaterials
compartments
tunable
properties.
Abstract
The
design
and
construction
of
synthetic
cells
–
human‐made
microcompartments
that
mimic
features
living
have
experienced
a
real
boom
in
the
past
decade.
While
many
efforts
been
geared
toward
assembling
membrane‐bounded
compartments,
coacervate
droplets
produced
by
liquid–liquid
phase
separation
emerged
as
an
alternative
membrane‐free
compartmentalization
paradigm.
Here,
dual
role
cell
research
is
discussed:
encapsulated
within
membrane‐enclosed
coacervates
act
surrogates
membraneless
organelles
ubiquitously
found
cells;
alternatively,
they
can
be
viewed
crowded
cytosol‐like
chassis
for
constructing
integrated
cells.
After
introducing
key
concepts
coacervation
illustrating
chemical
diversity
systems,
their
physicochemical
properties
resulting
bioinspired
functions
are
emphasized.
Moving
from
suspensions
free
floating
coacervates,
two
nascent
roles
these
highlighted:
organelle‐like
modules
templates.
Building
discussion
on
recent
studies
literature,
potential
to
assemble
capable
multiple
life‐inspired
showcased.
Future
challenges
still
tackled
field
finally
discussed.
Communications Chemistry,
Год журнала:
2023,
Номер
6(1)
Опубликована: Фев. 3, 2023
Liquid-liquid
phase
separation
(LLPS)
underlies
the
formation
of
intracellular
membraneless
compartments
in
biology
and
may
have
played
a
role
protocells
that
concentrate
key
chemicals
during
origins
life.
While
LLPS
simple
systems,
such
as
oil
water,
is
well
understood,
many
aspects
complex,
out-of-equilibrium
molecular
systems
remain
elusive.
Here,
author
discusses
open
questions
recent
insights
related
to
formation,
function
fate
condensates
both
cell
protocell
research.
Chemical Reviews,
Год журнала:
2024,
Номер
124(6), С. 3186 - 3219
Опубликована: Март 11, 2024
It
is
now
generally
accepted
that
macromolecules
do
not
act
in
isolation
but
"live"
a
crowded
environment,
is,
an
environment
populated
by
numerous
different
molecules.
The
field
of
molecular
crowding
has
its
origins
the
far
80s
became
only
end
90s.
In
present
issue,
we
discuss
various
aspects
are
influenced
and
need
to
consider
effects.
This
Review
meant
as
introduction
theme
analysis
evolution
concept
through
time
from
colloidal
polymer
physics
more
biological
perspective.
We
introduce
themes
will
be
thoroughly
treated
other
Reviews
issue.
our
intentions,
each
may
stand
itself,
complete
collection
aspiration
provide
complementary
perspectives
propose
holistic
view
crowding.
Annual Review of Biophysics,
Год журнала:
2024,
Номер
53(1), С. 319 - 341
Опубликована: Фев. 16, 2024
Biomolecular
condensates
are
highly
versatile
membraneless
organelles
involved
in
a
plethora
of
cellular
processes.
Recent
years
have
witnessed
growing
evidence
the
interaction
these
droplets
with
membrane-bound
structures.
Condensates'
adhesion
to
membranes
can
cause
their
mutual
molding
and
regulation,
is
fundamental
relevance
intracellular
organization
communication,
organelle
remodeling,
embryogenesis,
phagocytosis.
In
this
article,
we
review
advances
understanding
membrane-condensate
interactions,
focus
on
vitro
models.
These
minimal
systems
allow
precise
characterization
tuning
material
properties
both
provide
workbench
for
visualizing
resulting
morphologies
quantifying
interactions.
interactions
give
rise
diverse
biologically
relevant
phenomena,
such
as
molecular-level
restructuring
membrane,
nano-
microscale
ruffling
condensate-membrane
interface,
coupling
protein
lipid
phases.
Communications Chemistry,
Год журнала:
2024,
Номер
7(1)
Опубликована: Апрель 9, 2024
Dynamic
microscale
droplets
produced
by
liquid-liquid
phase
separation
(LLPS)
have
emerged
as
appealing
biomaterials
due
to
their
remarkable
features.
However,
the
instability
of
limits
construction
population-level
structures
with
collective
behaviors.
Here
we
first
provide
a
brief
background
in
context
materials
properties.
Subsequently,
discuss
current
strategies
for
stabilizing
including
physical
and
chemical
modulation.
We
also
recent
development
LLPS
various
applications
such
synthetic
cells
biomedical
materials.
Finally,
give
insights
on
how
stabilized
can
self-assemble
into
higher-order
displaying
coordinated
functions
fully
exploit
potentials
bottom-up
biology
applications.
Chemical Communications,
Год журнала:
2022,
Номер
58(80), С. 11183 - 11200
Опубликована: Янв. 1, 2022
In
this
Feature
Article,
we
analyze
how
a
minimal
cell
cycle
of
growth,
replication
genetic
information
and
division
could
be
realized
in
coacervate
protocells.
This
would
allow
such
primitive
cells
to
undergo
evolution
at
the
origins
life.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Сен. 28, 2023
Membrane
wetting
by
biomolecular
condensates
recently
emerged
as
a
key
phenomenon
in
cell
biology,
playing
an
important
role
diverse
range
of
processes
across
different
organisms.
However,
understanding
the
molecular
mechanisms
behind
condensate
formation
and
interaction
with
lipid
membranes
is
still
missing.
To
study
this,
we
exploited
properties
dyes
ACDAN
LAURDAN
nano-environmental
sensors
combination
phasor
analysis
hyperspectral
lifetime
imaging
microscopy.
Using
glycinin
model
condensate-forming
protein
giant
vesicles
membranes,
obtained
vital
information
on
process
membrane
wetting.
Our
results
reveal
that
display
differences
water
dynamics
when
changing
salinity
medium
consequence
rearrangements
secondary
structure
protein.
Remarkably,
membrane-condensates
well
polymer
indicated
correlation
between
increased
affinity
enhanced
packing.
This
demonstrated
decrease
dipolar
relaxation
all
membrane-condensate
systems,
suggesting
general
mechanism
to
tune
packing
