Photoswitchable Endocytosis of Biomolecular Condensates in Giant Vesicles DOI
Agustín Mangiarotti, Mina Aleksanyan, Macarena Siri

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(23)

Published: April 6, 2024

Abstract Interactions between membranes and biomolecular condensates can give rise to complex phenomena such as wetting transitions, mutual remodeling, endocytosis. In this study, light‐triggered manipulation of condensate engulfment is demonstrated using giant vesicles containing photoswitchable lipids. UV irradiation increases the membrane area, which be stored in nanotubes. When contact with a droplet, light triggers rapid endocytosis, reverted by blue light. The affinity protein‐rich reversibility processes quantified from confocal microscopy images. degree photo‐induced engulfment, whether partial or complete, depends on vesicle excess area relative sizes condensates. Theoretical estimates suggest that utilizing light‐induced increase vesicle‐condensate adhesion interface energetically more favorable than energy gain folding into invaginations tubes. overall findings demonstrate membrane‐condensate interactions easily quickly modulated via light, providing versatile system for building platforms control cellular events design intelligent drug delivery systems cell repair.

Language: Английский

Biomolecular condensates can both accelerate and suppress aggregation of α-synuclein DOI Creative Commons
Wojciech P. Lipiński, Brent S. Visser, Irina Robu

et al.

Science Advances, Journal Year: 2022, Volume and Issue: 8(48)

Published: Dec. 2, 2022

Biomolecular condensates present in cells can fundamentally affect the aggregation of amyloidogenic proteins and play a role regulation this process. While liquid-liquid phase separation by themselves act as an alternative nucleation pathway, interaction partly disordered aggregation-prone with preexisting that localization centers could be far more general mechanism altering their behavior. Here, we show so-called host biomolecular both accelerate slow down amyloid formation. We study protein α-synuclein two truncated variants presence three types composed nonaggregating peptides, RNA, or ATP. Our results demonstrate markedly speed up formation when localize to interface. However, also significantly suppress sequestering stabilizing proteins, thereby providing living possible protection against

Language: Английский

Citations

76
Wetting and complex remodeling of membranes by biomolecular condensates DOI Creative Commons
Agustín Mangiarotti, Nannan Chen, Ziliang Zhao

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: May 22, 2023

Abstract Cells compartmentalize parts of their interiors into liquid-like condensates, which can be reconstituted in vitro. Although these condensates interact with membrane-bound organelles, potential for membrane remodeling and the underlying mechanisms such interactions are not well-understood. Here, we demonstrate that between protein - including hollow ones, membranes lead to remarkable morphological transformations provide a theoretical framework describe them. Modulation solution salinity or composition drives condensate-membrane system through two wetting transitions, from dewetting, broad regime partial wetting, complete wetting. When sufficient area is available, fingering ruffling interface observed, an intriguing phenomenon producing intricately curved structures. The observed morphologies governed by interplay adhesion, elasticity, interfacial tension. Our results highlight relevance cell biology, pave way design synthetic membrane-droplet based biomaterials compartments tunable properties.

Language: Английский

Citations

75
Coacervate Droplets for Synthetic Cells DOI Creative Commons

Zi Lin,

Thomas Beneyton, Jean‐Christophe Baret

et al.

Small Methods, Journal Year: 2023, Volume and Issue: 7(12)

Published: July 18, 2023

Abstract The design and construction of synthetic cells – human‐made microcompartments that mimic features living have experienced a real boom in the past decade. While many efforts been geared toward assembling membrane‐bounded compartments, coacervate droplets produced by liquid–liquid phase separation emerged as an alternative membrane‐free compartmentalization paradigm. Here, dual role cell research is discussed: encapsulated within membrane‐enclosed coacervates act surrogates membraneless organelles ubiquitously found cells; alternatively, they can be viewed crowded cytosol‐like chassis for constructing integrated cells. After introducing key concepts coacervation illustrating chemical diversity systems, their physicochemical properties resulting bioinspired functions are emphasized. Moving from suspensions free floating coacervates, two nascent roles these highlighted: organelle‐like modules templates. Building discussion on recent studies literature, potential to assemble capable multiple life‐inspired showcased. Future challenges still tackled field finally discussed.

Language: Английский

Citations

49
Open questions on liquid–liquid phase separation DOI Creative Commons
Evan Spruijt

Communications Chemistry, Journal Year: 2023, Volume and Issue: 6(1)

Published: Feb. 3, 2023

Liquid-liquid phase separation (LLPS) underlies the formation of intracellular membraneless compartments in biology and may have played a role protocells that concentrate key chemicals during origins life. While LLPS simple systems, such as oil water, is well understood, many aspects complex, out-of-equilibrium molecular systems remain elusive. Here, author discusses open questions recent insights related to formation, function fate condensates both cell protocell research.

Language: Английский

Citations

43
Molecular Crowding: The History and Development of a Scientific Paradigm DOI Creative Commons
Caterina Alfano, Yann Fichou, Klaus Huber

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(6), P. 3186 - 3219

Published: March 11, 2024

It is now generally accepted that macromolecules do not act in isolation but "live" a crowded environment, is, an environment populated by numerous different molecules. The field of molecular crowding has its origins the far 80s became only end 90s. In present issue, we discuss various aspects are influenced and need to consider effects. This Review meant as introduction theme analysis evolution concept through time from colloidal polymer physics more biological perspective. We introduce themes will be thoroughly treated other Reviews issue. our intentions, each may stand itself, complete collection aspiration provide complementary perspectives propose holistic view crowding.

Language: Английский

Citations

37
Biomolecular Condensates in Contact with Membranes DOI
Agustín Mangiarotti, Rumiana Dimova

Annual Review of Biophysics, Journal Year: 2024, Volume and Issue: 53(1), P. 319 - 341

Published: Feb. 16, 2024

Biomolecular condensates are highly versatile membraneless organelles involved in a plethora of cellular processes. Recent years have witnessed growing evidence the interaction these droplets with membrane-bound structures. Condensates' adhesion to membranes can cause their mutual molding and regulation, is fundamental relevance intracellular organization communication, organelle remodeling, embryogenesis, phagocytosis. In this article, we review advances understanding membrane-condensate interactions, focus on vitro models. These minimal systems allow precise characterization tuning material properties both provide workbench for visualizing resulting morphologies quantifying interactions. interactions give rise diverse biologically relevant phenomena, such as molecular-level restructuring membrane, nano- microscale ruffling condensate-membrane interface, coupling protein lipid phases.

Language: Английский

Citations

25
Self-assembly of stabilized droplets from liquid–liquid phase separation for higher-order structures and functions DOI Creative Commons
Mehwish Naz,

Lin Zhang,

Chong Chen

et al.

Communications Chemistry, Journal Year: 2024, Volume and Issue: 7(1)

Published: April 9, 2024

Dynamic microscale droplets produced by liquid-liquid phase separation (LLPS) have emerged as appealing biomaterials due to their remarkable features. However, the instability of limits construction population-level structures with collective behaviors. Here we first provide a brief background in context materials properties. Subsequently, discuss current strategies for stabilizing including physical and chemical modulation. We also recent development LLPS various applications such synthetic cells biomedical materials. Finally, give insights on how stabilized can self-assemble into higher-order displaying coordinated functions fully exploit potentials bottom-up biology applications.

Language: Английский

Citations

17
Growth, replication and division enable evolution of coacervate protocells DOI Creative Commons
Annemiek D. Slootbeek, Merlijn H. I. van Haren, Iris B. A. Smokers

et al.

Chemical Communications, Journal Year: 2022, Volume and Issue: 58(80), P. 11183 - 11200

Published: Jan. 1, 2022

In this Feature Article, we analyze how a minimal cell cycle of growth, replication genetic information and division could be realized in coacervate protocells. This would allow such primitive cells to undergo evolution at the origins life.

Language: Английский

Citations

47
Biomolecular condensates modulate membrane lipid packing and hydration DOI Creative Commons
Agustín Mangiarotti, Macarena Siri, Nicky W. Tam

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Sept. 28, 2023

Membrane wetting by biomolecular condensates recently emerged as a key phenomenon in cell biology, playing an important role diverse range of processes across different organisms. However, understanding the molecular mechanisms behind condensate formation and interaction with lipid membranes is still missing. To study this, we exploited properties dyes ACDAN LAURDAN nano-environmental sensors combination phasor analysis hyperspectral lifetime imaging microscopy. Using glycinin model condensate-forming protein giant vesicles membranes, obtained vital information on process membrane wetting. Our results reveal that display differences water dynamics when changing salinity medium consequence rearrangements secondary structure protein. Remarkably, membrane-condensates well polymer indicated correlation between increased affinity enhanced packing. This demonstrated decrease dipolar relaxation all membrane-condensate systems, suggesting general mechanism to tune packing

Language: Английский

Citations

39
How to drink like a liposome DOI
Jianhui Liu, Ben Zhong Tang

Nature Reviews Chemistry, Journal Year: 2023, Volume and Issue: 7(1), P. 5 - 6

Published: Jan. 2, 2023

Language: Английский

Citations

37