Bioconjugate Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 9, 2025
Nanobodies
play
an
increasingly
prominent
role
in
cancer
imaging
and
therapy.
However,
their
vivo
efficacy
is
often
constrained
by
inadequate
tumor
penetration
rapid
clearance
from
the
bloodstream,
particularly
brain
tumors
due
to
intractable
blood–brain
barrier
(BBB).
Glycosylation
a
favorable
strategy
for
modulating
biological
functions
of
nanobodies,
including
permeability
pharmacokinetics,
but
it
also
leads
heterogeneous
glycan
structures,
which
affect
targeting
ability,
stability,
quality
nanobodies.
Here,
we
describe
post-translational
modification
produce
precisely
engineered
homogeneous
nanobody–glucoside
conjugates
effective
BBB
targeting.
Specifically,
employ
enzymatic
method
click
chemistry
functionalize
nanobodies
with
glucoside
poly(ethylene
glycol)
(PEG),
facilitating
efficient
transcytosis
into
via
glucose
transporter-1
(GLUT1).
Furthermore,
rationally
select
near-infrared
(NIR)
fluorophore
labeling
maintain
metabolic
pathway
biodistribution
assess
potency
two
models.
The
resulting
demonstrate
remarkable
increase
accumulation,
are
∼2.9-fold
higher
transgenic
mouse
model
∼5.7-fold
orthotopic
glioma
compared
unmodified
This
study
provides
promising
approach
production
nanobody
therapeutic
agents
central
nervous
system
(CNS)
delivery.
Inorganic Chemistry,
Год журнала:
2024,
Номер
63(7), С. 3402 - 3410
Опубликована: Фев. 8, 2024
An
efficient
synthesis
of
3-pyrrolylBODIPY
dyes
has
been
developed
from
a
rational
mixture
various
aromatic
aldehydes
and
pyrrole
in
straightforward
condensation
reaction,
followed
by
situ
successively
oxidative
nucleophilic
substitution
using
one-pot
strategy.
These
resultant
3-pyrrolylBODIPYs
without
blocking
substituents
not
only
exhibit
the
finely
tunable
photophysical
properties
induced
flexible
meso-aryl
but
also
serve
as
valuable
synthetic
framework
for
further
selective
functionalization.
As
proof
such
potential,
one
dye
(581/603
nm)
through
installation
morpholine
group
is
applicable
lysosome-targeting
imaging.
Furthermore,
an
ethene-bridged
3,3′-dipyrrolylBODIPY
dimer
was
constructed,
which
displayed
near-infrared
(NIR)
emission
extended
to
1200
nm
with
large
fluorescence
brightness
(2840
M–1
cm–1).
The
corresponding
nanoparticles
(NPs)
afforded
high
photothermal
conversion
efficiency
(PCE)
value
72.5%,
eventually
resulting
favorable
photocytotoxicity
(IC50
=
9.4
μM)
vitro
eradication
HeLa
cells
under
808
laser
irradiation,
highlighting
their
potential
application
therapy
NIR
window.
Chemical Communications,
Год журнала:
2024,
Номер
60(45), С. 5770 - 5789
Опубликована: Янв. 1, 2024
This
highlight
reviews
various
strategies
for
constructing
BODIPY-based
photocages,
their
photodecaging
mechanisms,
and
applications
in
the
biomedical
field.
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 19, 2024
Abstract
Despite
the
extensive
reporting
of
versatile
aggregation‐induced
emission
luminogens
(AIEgens),
boosting
their
molar
extinction
coefficient
(MEC,
ε
≈
10
3
–10
4
M
−1
cm
)
remains
a
formidable
challenge,
which
poses
significant
obstacle
to
achieving
ceiling
output
performance.
In
this
work,
an
“aggrandizing
absorption
reservoir”
approach
is
proposed
designing
AIEgens
with
remarkable
photons‐harvesting
ability,
ultimately
enlarging
its
performance
in
terms
short‐wave
infrared
(SWIR,
900–1700
nm)
and
photothermal
effect.
For
first
time,
(bis‐(borondifluoride)‐8‐imidazodipyrromethene)
(BOIMPY)
unit,
provides
sufficient
rigidity
strong
electron‐withdrawing
ability
by
employing
benzimidazole
as
bridging
ligand
binding
two
BF
2
units,
utilized
fabricate
novel
AIEgen
(TPEB)
that
exhibits
ultrahigh
MEC
up
1.29
×
5
at
828
nm.
To
best
knowledge,
highest
reported
among
near‐infrared
absorption.
Thanks
photon‐harvesting
TPEB
fluorescence
SWIR
region
good
The
as‐prepared
NPs
are
successfully
applied
for
imaging‐guided
antitumor
therapy.
guideline
biomedical
applications.
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(34)
Опубликована: Июнь 10, 2024
Abstract
J‐aggregation
brings
intriguing
optical
and
electronic
properties
to
molecular
dyes
significantly
expands
their
applicability
across
diverse
domains,
yet
the
challenge
for
rationally
designing
J‐aggregating
persists.
Herein,
we
developed
a
large
number
of
from
scratch
by
progressively
refining
structure
common
heptamethine
cyanine.
J‐aggregates
with
sharp
spectral
bands
(full‐width
at
half‐maximum≤38
nm)
are
attained
introducing
branched
featuring
benzyl
trifluoroacetyl
group
meso‐position
dyes.
Fine‐tuning
enables
regulation
J‐aggregates.
Analysis
single
crystal
data
nine
reveals
correlation
between
propensity
arrangement
within
crystals.
Some
successfully
implemented
in
multiplexed
optoacoustic
fluorescence
imaging
animals.
Notably,
three‐color
multispectral
tomography
high
spatiotemporal
resolution
is
achieved,
owing
distinct
absorption
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(19)
Опубликована: Март 11, 2024
Abstract
Nitric
oxide
(NO)
exhibits
both
pro‐
and
anti‐tumor
effects.
Therefore,
real‐time
in
vivo
imaging
quantification
of
tumor
NO
dynamics
are
essential
for
understanding
the
conflicting
roles
played
pathophysiology.
The
current
molecular
probes,
however,
cannot
provide
high‐resolution
deep
tissues,
making
them
unsuitable
these
purposes.
Herein,
we
designed
a
photoacoustic
probe
with
an
absorption
maximum
beyond
1000
nm
high
spatial
quantitative
dynamics.
remarkable
sensitivity,
selective
ratiometric
response
behavior,
good
tumor‐targeting
abilities,
facilitating
throughout
progression
micron‐resolution
level.
Using
as
agent,
successfully
quantified
tumor,
liver
kidney.
We
have
pinpointed
concentration
threshold
around
80
nmol/cm
3
NO,
which
plays
crucial
role
“double‐edged‐sword”
function
tumors.
Furthermore,
revealed
reciprocal
relationship
between
tumors
that
liver,
providing
initial
insights
into
possible
NO‐mediated
communication
liver.
believe
will
help
resolve
aspects
biology
guide
design
agents
diagnosis
anti‐cancer
drug
screening.
