Fluorescent Probe for Investigating the Mitochondrial Viscosity and Hydrogen Peroxide Changes in Cerebral Ischemia/Reperfusion Injury

Analytical Chemistry, Год журнала: 2024, Номер 96(8), С. 3436 - 3444

Опубликована: Фев. 19, 2024

Cerebral ischemia–reperfusion injury (CIRI), a cause of cerebral dysfunction during infarction treatment, is closely associated with mitochondrial viscosity and hydrogen peroxide (H2O2). However, the accurate measurement H2O2 levels in CIRI challenging because lack sufficient selectivity blood–brain barrier (BBB) penetration existing monitoring tools related to CIRI, hampering exploration role CIRI. To address this issue, we designed an activatable fluorescent probe, mitochondria-targeting styryl-quinolin-ium (Mito-IQS), excellent properties including high selectivity, targeting, BBB penetration, for visualization brain. Based on real-time capabilities bursts were visualized This probe can be used monitor therapeutic effects butylphthalein treatment. More importantly, vivo experiments further confirmed that was levels. discovery provides new insights study expected accelerate process diagnosis, drug design.

Язык: Английский

---

Aggregation-Induced Emission Luminogen: Role in Biopsy for Precision Medicine

Chemical Reviews, Год журнала: 2024, Номер 124(20), С. 11242 - 11347

Опубликована: Окт. 9, 2024

Biopsy, including tissue and liquid biopsy, offers comprehensive real-time physiological pathological information for disease detection, diagnosis, monitoring. Fluorescent probes are frequently selected to obtain adequate on processes in a rapid minimally invasive manner based their advantages biopsy. However, conventional fluorescent have been found show aggregation-caused quenching (ACQ) properties, impeding greater progresses this area. Since the discovery of aggregation-induced emission luminogen (AIEgen) promoted advancements molecular bionanomaterials owing unique high quantum yield (QY) signal-to-noise ratio (SNR),

Язык: Английский

---

Exogenously and Endogenously Sequential Regulation of DNA Nanodevices Enables Organelle‐Specific Signal Amplification in Subcellular ATP Profiling

Angewandte Chemie International Edition, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Abstract Adenosine triphosphate (ATP), the primary energy currency in cells, is dynamically regulated across different subcellular compartments. The ATP interplay between mitochondria and endoplasmic reticulum (ER) underscores their coordinated roles various biochemical processes, highlighting necessity for precise profiling of dynamics. Here we present an exogenously endogenously dual‐regulated DNA nanodevice spatiotemporally selective, subcellular‐compartment specific signal amplification sensing. system allows exogenous NIR light‐controlled spatiotemporal localization activation aptamer sensor or ER, while a endogenous enzyme organelles further drives via consumption molecular beacon fuels, resulting significantly enhanced sensitivity spatial precision organelle interest. Furthermore, demonstrate application this robust monitoring fluctuations ER following drug interventions. This advancement provides powerful tool improving our understanding cellular energetics at level holds potential development targeted therapeutics.

Язык: Английский

---

Advances in Organic Small Molecule-Based Fluorescent Probes for Precision Detection of Liver Diseases: A Perspective on Emerging Trends and Challenges

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Liver disease poses a significant challenge to global health, and its early diagnosis is crucial for improving treatment outcomes patient prognosis. Since fluctuation of key biomarkers during the onset progression liver diseases can directly reflect health normal/abnormal function, biomarker-based assays are vital tools detection disease. In this context, small molecule fluorescent probes have undeniably emerged as indispensable analysis, with an ever-growing number molecule-based being developed over recent years, sole aim monitoring relevant This perspective will focus on development application primarily last 10 years diagnosing range disease-related processes. It outline foundational design strategies developing promising probes, their optical response biomarkers, how they been demonstrated in proof-of-concept imaging applications. Current challenges new developments field be discussed, providing insights highlighting opportunities field.

Язык: Английский

---

Real-Time Monitoring of ONOO⁻ in Cerebral Ischemia-Reperfusion Injury Mouse Models Using a Hydrazine-Based NIR Fluorescent Probe

Redox Biology, Год журнала: 2025, Номер 80, С. 103494 - 103494

Опубликована: Янв. 13, 2025

Accurate and selective techniques for visualizing endogenous peroxynitrite (ONOO-) in cerebral ischemia-reperfusion injury (CIRI) models are essential understanding its complex pathological processes. Here, we introduced a longwave fluorescent probe TJO detecting ONOO- rapidly sensitively, with low detection limit of 91 nM. Furthermore, exhibits excellent fluorescence imaging capabilities, enabling detailed visualization ONOO⁻ CIRI mice model. This highlights potential real-time monitoring ONOO⁻-related conditions.

Язык: Английский

---

Advances in dual-sensing bioprobes for simultaneous monitoring ATP and various biological species

Coordination Chemistry Reviews, Год журнала: 2024, Номер 510, С. 215800 - 215800

Опубликована: Март 28, 2024

Язык: Английский

---

Dual-Channel/Localization Single-Molecule Fluorescence Probe for Monitoring ATP and HOCl in Early Diagnosis and Therapy of Rheumatoid Arthritis

Analytical Chemistry, Год журнала: 2024, Номер 96(14), С. 5428 - 5436

Опубликована: Март 29, 2024

Rheumatoid arthritis (RA), a common chronic inflammatory illness, is still incurable, reducing the sufferers' quality of life significantly. Adenosine 5′-triphosphate (ATP) and hypochlorous acid (HOCl) are key indicators in RA, but their precise mechanisms RA pathophysiology unknown. As result, order to detect ATP HOCl simultaneously, we created two new dual-channel/localization single-molecule fluorescence probes, RhTNMB RhFNMB. Furthermore, RhFNMB outperformed terms detection performance. produce independent responses light red channel (λex = 520 nm, λem 586 nm) deep 620 688 nm), respectively, without spectral crosstalk. It should be noted that probe successfully imaged mitochondria cells. Surprisingly, demonstrated remarkable ability diagnosis treatment Pseudomonas aeruginosa-induced abdominal inflammation mice. We continued apply track discovered concentrations were considerably greater joints than normal joints. also confirmed therapeutic effect methotrexate on RA. This study first achieve dual-channel imaging HOCl, which great value for early therapy

Язык: Английский

---

A novel coumarin-linked tetraphenylethene fluorescent probe for simultaneous sensing of ATP and GSH

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 412, С. 135772 - 135772

Опубликована: Апрель 7, 2024

Язык: Английский

---

Involvement of intestinal mucosal microbiota in adenine-induced liver function injury

3 Biotech, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 12, 2024

Язык: Английский

---

Nitrile-aminothiol bioorthogonal near-infrared fluorogenic probes for ultrasensitive in vivo imaging

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 2, 2025

Bioorthogonal chemistry-mediated self-assembly holds great promise for dynamic molecular imaging in living organisms. However, existing approaches are limited to nanoaggregates with 'always-on' signals, suffering from high signal-to-background ratio (SBR) and compromised detection sensitivity. Herein we report a nitrile-aminothiol (NAT) bioorthogonal fluorogenic probe (CyNAP-SS-FK) ultrasensitive diagnosis of orthotopic hepatocellular carcinoma. This comprises nitrile-substituted hemicyanine scaffold cysteine tail dually locked biomarker-responsive moieties. Upon dual cleavage by tumor-specific cathepsin B biothiols, the 1,2-aminothiol residue is exposed spontaneously reacts nitrile group situ intramolecular macrocyclization, enabling near-infrared fluorescence (NIRF) turn-on as well self-assembly. In male mice, such 'cleavage-click-assembly' regimen allows real-time small cancerous lesions (~2 mm diameter) improved SBR (~5) extended window (~36 h), outperforming conventional clinical assays. study not only presents NAT click reaction-based probes but also highlights generic dual-locked design these probes. Existing can suffer low Here, authors sensing action lesions.

Язык: Английский

---