Nature
has
perfected
the
reversible
control
over
activity
of
molecules
and
biomolecules
in
human
body.
Photocages
aim
to
mimic
this
space
time
using
light
as
a
trigger,
field
witnessed
many
excellent
contributions
that
extend
their
use
tissue-penetrating
region.
Yet
little
attention
been
paid
developing
simple
caging
strategies
which
are
crucial
translate
photocages
into
widely
accepted
tool
beyond
chemical
space.
Here,
we
report
robust
user-friendly
protocol
enables
installation
amine,
thiol
phenol
payloads
single
step
under
mild
conditions
bench-stable
intermediates.
The
displays
compatibility
with
payloads,
manifested
by
hormones,
neurotransmitters,
tripeptide
highly
complex
FDA-approved
drugs,
including
antibiotics
or
anti-cancer
agents.
In
proof-of-concept
study,
utilize
strategy
cage
clinically
used
CDK4/6
inhibitor
palbociclib
tumor
suppressing
retinoblastoma
protein
breast
cancer
cells
near-infrared
(NIR)
light.
We
anticipate
normalizing
access
NIR-absorbing
through
will
empower
research
across
various
scientific
disciplines.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июль 9, 2024
Cultured
meat
is
emerging
as
a
new
type
of
food
that
can
provide
animal
protein
in
sustainable
way.
Many
previous
studies
employed
various
types
scaffolds
to
develop
cultured
with
similar
properties
slaughtered
meat.
However,
important
such
flavor
were
not
discussed,
even
though
they
determine
the
quality
food.
Flavor
characteristics
vary
dramatically
depending
on
amount
and
amino
acids
sugars
produce
volatile
compounds
through
Maillard
reaction
upon
cooking.
In
this
study,
flavor-switchable
scaffold
developed
release
meaty
only
cooking
temperature
mimicking
By
introducing
switchable
compound
(SFC)
into
gelatin-based
hydrogel,
we
fabricate
functional
enhance
aromatic
The
temperature-responsive
SFC
stably
remains
during
cell
culture
period
be
released
at
temperature.
Surprisingly,
fabricated
exhibits
pattern
beef.
This
research
suggests
strategy
enhanced
sensorial
by
developing
which
mimic
natural
flavors
conventional
JACS Au,
Год журнала:
2024,
Номер
4(7), С. 2687 - 2694
Опубликована: Июль 5, 2024
Hydrogen
sulfide
(H2S)
is
a
gaseous
signaling
molecule,
exerting
crucial
regulatory
functions
in
organelles
and
cellular
environments.
H2S
exhibits
high
therapeutic
potential
synergistic
effects
with
other
drugs,
its
potency
notably
enhanced
through
organelle-specific
targeting.
Yet,
the
navigation
of
light-activated
donors
to
specific
remains
absent.
Here,
we
report
first
photocage
that
simultaneously
delivers
payload
subcellular
precision
mitochondria
live
human
cells
using
tissue-penetrating
near-infrared
light
as
trigger.
The
fluorogenic
enables
real-time
monitoring
process,
demonstrate
concurrent
uncaging
combination
fluorescence
microscopy
mitochondria-specific
fluorescent
probes.
We
anticipate
these
photocages
will
permit
precise
delivery
H2S-drug
combinations
exceptional
spatiotemporal
control,
thereby
driving
harnessing
known
discovery
novel
strategies.
ABSTRACT
Photodynamic
therapy
(PDT)
and
photothermal
(PTT)
have
emerged
promising
applications
in
both
fundamental
research
clinical
trials.
However,
it
remains
challenging
to
develop
ideal
photosensitizers
(PSs)
that
concurrently
integrate
high
photostability,
large
near‐infrared
absorptivity,
efficient
therapeutic
capabilities.
Herein,
we
reported
a
sample
engineering
strategy
afford
benzene‐fused
Cy5
dimer
(
Cy‐D‐5
)
for
synergistically
boosting
PDT/PTT
applications.
Intriguingly,
exhibits
tendency
form
J
‐aggregates
H
phosphate‐buffered
saline,
which
show
long‐wavelength
absorption
band
bathochromically
shifted
810
nm
short‐wavelength
hypsochromically
745
nm,
respectively,
when
compared
its
behavior
ethanol
(778
nm).
Density‐functional
theory
calculations
combined
with
time‐resolved
transient
optical
spectroscopy
analysis
reveal
the
fused
low
Δ
E
ST
(0.51
eV)
non‐radiative
transition
rates
(12.6
times
greater
than
of
clinically
approved
PS‐indocyanine
green
[PS‐
ICG
]).
Furthermore,
demonstrates
higher
photosensitizing
ability
produce
1
O
2
,
stronger
conversion
efficiency
η
=
64.4%),
photostability
.
These
properties
enable
achieve
complete
tumor
ablation
upon
808
laser
irradiation,
highlighting
potential
as
powerful
dual‐function
phototherapeutic
agent.
This
work
may
offer
practical
other
existing
dyes
red‐shifted
spectral
range
various
phototherapy
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Апрель 18, 2025
Over
the
past
decade,
field
of
cell
therapy
has
rapidly
expanded
with
aim
to
replace
and
repair
damaged
cells
and/or
tissue.
Depending
on
disease
many
different
types
can
be
used
as
part
such
a
therapy.
Here
we
focused
potential
treatment
myocardial
infarction,
where
currently
available
options
are
not
able
regenerate
loss
healthy
heart
We
generated
good
manufacturing
practice
(GMP)-compatible
cardiomyocytes
(iCMs)
from
transgene-
xenofree
induced
pluripotent
stem
(iPSCs)
that
seamless
adapted
for
clinical
applications.
Further
protocols
were
established
replating
freezing/thawing
iCMs
under
conditions.
showed
cardiac
phenotype,
expression
specific
markers
absence
pluripotency
at
RNA
protein
level.
To
ensure
pure
population
in
vivo
applications,
minimized
risks
iPSC
contamination
using
RNA-switch
technology
safety.
describe
generation
further
processing
xeno-
transgene-free
iCMs.
The
use
GMP-compliant
differentiation
ab
initio
facilitates
translation
this
project
later
stages.
Organic & Biomolecular Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
We
highlight
the
recent
progress
made
towards
development
of
visible-to-NIR
activatable
photolabile
caging
groups,
in
which
strategies
used
to
red-shift
absorption
spectra,
improve
photolysis
efficiency
and
aqueous
solubility
are
described.
