ROPISA Strategy for In‐Situ Loading in Polypeptide Nanoparticles DOI
Rahul Nisal,

Parshuram Kambale,

Shahidkhan Pathan

и другие.

Chemistry - An Asian Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

Abstract We report a ring‐opening polymerization induced self‐assembly (ROPISA) synthetic strategy for in‐situ encapsulation of fluorescent dye molecules in poly(ʟ‐serine) based polypeptide nano‐assemblies and demonstrate their cellular bioimaging application. A bulky ʟ‐serine N‐carboxyanhydride monomer is tailor‐made polymerized using PEG‐amine as hydrophilic macroinitiator water at pH 8.5 to obtain block copolymer stable dispersions the form opalescent solutions. Both soluble dyes like Rhodamine B, HPTS insoluble Nile red are readily encapsulated ROPISA process which afforded nanoformulation direct application biological system. The nanoparticle dispersion found be stable, they have spherical morphology 25 nm size. nascent nanoparticles were nontoxic mammalian cells up 100 µg/mL non‐hemolytic Red Blood Cells. These endocytosed across cell membrane internalized cytosol, proof‐of‐concept was established by confocal microscopy. This newly developed loading opens new platform nano‐formulations both material biomedical fields.

Язык: Английский

Recent Advances and Future Developments in the Preparation of Polypeptides via N-Carboxyanhydride (NCA) Ring-Opening Polymerization DOI
Yueming Wu, Kang Chen, Jiangzhou Wang

и другие.

Journal of the American Chemical Society, Год журнала: 2024, Номер 146(35), С. 24189 - 24208

Опубликована: Авг. 22, 2024

Polypeptides have the same or similar backbone structures as proteins and peptides, rendering them suitable important biomaterials. Amino acid N-carboxyanhydrides (NCA) ring-opening polymerization has been most efficient strategy for polypeptide preparation, with continuous advance in design of initiators, catalysts reaction conditions. This Perspective first summarizes recent progress NCA synthesis purification. Subsequently, we focus on various initiators polymerization, accelerating enhancing controllability advances approach polymerization. Finally, discuss future research directions open challenges.

Язык: Английский

Процитировано

21

Integrating synthetic polypeptides with innovative material forming techniques for advanced biomedical applications DOI Creative Commons

Dandan Kang,

Yu Zhang,

Deng‐Guang Yu

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 12, 2025

Polypeptides are highly valued in biomedical science for their biocompatibility and biodegradability, making them valuable drug delivery, tissue engineering, antibacterial dressing. The diverse design of polymer chains self-assembly techniques allow different side secondary structures, enhancing potential. However, the traditional solid powder form polypeptides presents challenges skin applications, shipping, recycling, limiting practical utility. Recent advancements material forming methods polypeptide synthesis have produced biomaterials with uniform, distinct shapes, improving usability. This review outlines progress material-forming over past decade. main include solid-phase ring-opening polymerization N-carboxyanhydrides while like electrospinning, 3D printing, coating explored. Integrating structural these is emphasized, leading to materials unique shapes. also identifies research hotspots using VOSviewer software, which visually presented circular packing images. It further discusses emerging applications such as wound healing, emphasizing crucial role shape performance. concludes by exploring future trends developing shapes advanced encouraging research.

Язык: Английский

Процитировано

2

Synthetic Polymers for Drug, Gene, and Vaccine Delivery DOI Creative Commons
Yajun Xu, Jie Chen, Jianxun Ding

и другие.

Polymer science & technology., Год журнала: 2025, Номер unknown

Опубликована: Апрель 25, 2025

Язык: Английский

Процитировано

1

Chiral polymer micro/nano-objects: evolving preparation strategies in heterogeneous polymerization DOI Creative Commons
Dongdong Liu,

Jinyan Zhao,

Xi Zhao

и другие.

Science China Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 13, 2024

Язык: Английский

Процитировано

5

Supramolecular chirality capture in solvent monomer-based co-assemblies via in situ photopolymerization DOI

Zefeng Long,

Shuyuan Zheng, Weiqiang Zhou

и другие.

Chemical Communications, Год журнала: 2024, Номер 60(68), С. 9054 - 9057

Опубликована: Янв. 1, 2024

The supramolecular chirality of a co-assembly pyridine-cholesterol gelator and solvent monomer is captured via in situ photopolymerization. Doping with achiral dyes enables the creation multi-color CPL polymer films inverted handedness.

Язык: Английский

Процитировано

3

Strong Bioadhesives from Helical Polypeptides DOI

Jiangyan Shi,

Liufen Kong,

Ning Wang

и другие.

ACS Macro Letters, Год журнала: 2025, Номер 14(3), С. 299 - 305

Опубликована: Фев. 21, 2025

Bioadhesives have emerged as versatile and powerful tools for tissue repair integration with biomedical devices, offering a wide range of applications that captured significant clinical scientific interest. Synthetic polypeptide adhesives are particularly promising candidates bioadhesives, but often face limitations in adhesive strength. In this study, inspired by marine proteins, the secondary structure hydrophobic–hydrophilic balance polypeptides were precisely regulated to transform polyelectrolyte strong adhesive. The resulting demonstrated an strength exceeding 1.0 MPa, more than 10× higher previously reported synthetic cohesion adhesion can be optimized adjusting content hydrophobic residue ratios. More helices enhance interactions between backbone side chains well substrates. addition, these exhibit excellent tolerance acids or alkalis, remarkable variable materials tissues, impressive sealing performance.

Язык: Английский

Процитировано

0

Bimetallic Catalysts Based on Chiral Amplification, Inversion, and Assembly for an Asymmetric Cascade Process DOI
Xinjuan Li, Ao Li, Xiao Yu Tian

и другие.

Опубликована: Апрель 8, 2025

Abstract Designing heterogeneous catalysts to mimic complicated biological processes and exploring methods precisely modulate their chirality is crucial for asymmetric reactions. In this study, two chiral were immobilized on the non-helical C-C polymers, achieving amplification reversible reversal of polymer by altering copolymer structure solvent. Additionally, undergoes a layered assembly process with solvent changes, resulting in regulation its superhelical fiber (P or M configuration). At high concentrations, it aggregates form knitting wool ball-like hyperhelical three-dimensional structure. A strong correlation has been established between catalytic selectivity catalyst decarboxylative Mannich reaction/asymmetric transfer hydrogenation enantioselective cascade reaction. This bimetallic produces products reactions activity excellent enantioselectivity, "one stone, birds" effect. It can be reused 10 times without significant losss stereoselectivity. copolymerization synthesis strategy may contribute development new controllable

Язык: Английский

Процитировано

0

Facile Preparation of Crosslinked PEG‐Polypeptide Drug Nanocarriers Through Biphasic Polymerization of Crude N‐Carboxyanhydrides DOI
Shijie Wang, Shanshan Xiao, Ning Li

и другие.

Macromolecular Bioscience, Год журнала: 2025, Номер unknown

Опубликована: Апрель 11, 2025

Abstract Poly(ethylene glycol) (PEG)‐polypeptide drug nanocarriers have garnered increasing attention during last decades. However, the tedious polymer synthesis and stability issue greatly limit their further development. Herein biphasic polymerization platform is reported for easy access of a series crosslinked PEG‐polypeptide nanoparticles enhanced loading selective release. The accelerated allows preparation library PEG‐polypeptides from crude monomers with different polypeptide segments structural optimization. Nanocarriers redox‐responsive de‐crosslinking are therefore obtained based on model drug, doxorubicin, which exhibits killing cancer cells. This study provides synthetic method efficient nanocarriers, benefiting downstream therapeutic studies.

Язык: Английский

Процитировано

0

ROPISA Strategy for In‐Situ Loading in Polypeptide Nanoparticles DOI
Rahul Nisal,

Parshuram Kambale,

Shahidkhan Pathan

и другие.

Chemistry - An Asian Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

Abstract We report a ring‐opening polymerization induced self‐assembly (ROPISA) synthetic strategy for in‐situ encapsulation of fluorescent dye molecules in poly(ʟ‐serine) based polypeptide nano‐assemblies and demonstrate their cellular bioimaging application. A bulky ʟ‐serine N‐carboxyanhydride monomer is tailor‐made polymerized using PEG‐amine as hydrophilic macroinitiator water at pH 8.5 to obtain block copolymer stable dispersions the form opalescent solutions. Both soluble dyes like Rhodamine B, HPTS insoluble Nile red are readily encapsulated ROPISA process which afforded nanoformulation direct application biological system. The nanoparticle dispersion found be stable, they have spherical morphology 25 nm size. nascent nanoparticles were nontoxic mammalian cells up 100 µg/mL non‐hemolytic Red Blood Cells. These endocytosed across cell membrane internalized cytosol, proof‐of‐concept was established by confocal microscopy. This newly developed loading opens new platform nano‐formulations both material biomedical fields.

Язык: Английский

Процитировано

0