An All‐Round Athlete on the Track of Phototheranostics: Subtly Regulating the Balance between Radiative and Nonradiative Decays for Multimodal Imaging‐Guided Synergistic Therapy

Advanced Materials, Год журнала: 2020, Номер 32(36)

Опубликована: Июль 21, 2020

Abstract Aiming to achieve versatile phototheranostics with the integrated functionalities of multiple diagnostic imaging and synergistic therapy, optimum use dissipated energy through both radiative nonradiative pathways is definitely appealing, yet a significantly challenging task. To best knowledge, there have been no previous reports on single molecular species effective at affording all phototheranostic modalities including fluorescence (FLI), photoacoustic (PAI), photothermal (PTI), photodynamic therapy (PDT), (PTT). Herein, simple highly powerful one‐for‐all based aggregation‐induced emission (AIE)‐active fluorophores tactfully designed constructed. Thanks its strong electron donor–acceptor interaction finely modulated intramolecular motion, AIE fluorophore‐based nanoparticles simultaneously exhibit bright near‐infrared II (NIR‐II) emission, efficient reactive oxygen generation, high conversion efficiency upon NIR irradiation, indicating actualization balance between dissipations. Furthermore, unprecedented performance NIR‐II FLI‐PAI‐PTI trimodal‐imaging‐guided PDT–PTT demonstrated by precise tumor diagnosis complete elimination outcomes. This study thus brings new insight into development superior for practical cancer theranostics.

Язык: Английский

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Aminopeptidase N Activatable Fluorescent Probe for Tracking Metastatic Cancer and Image-Guided Surgery via in Situ Spraying

Journal of the American Chemical Society, Год журнала: 2020, Номер 142(13), С. 6381 - 6389

Опубликована: Март 13, 2020

The recurrence of malignant tumors is mostly caused by incompleted surgical resection. Especially, it difficult for surgeons to detect and accurately remove metastatic predominantly using visual examination palpation owing the lack effective means specifically distinguish boundary range between normal tumor tissues. Thus, development activated fluorescent probe with superior tumor-to-normal (T/N) tissue ratios particularly urgent in clinics. In view CD13/aminopeptidase N (APN) regarded as a cancer-specific biomarker, mediating progression, invasion, migration tumor, herein, we reported an APN-responsive YH-APN demonstrated its application cancer cells. Through situ spraying manner, (subcutaneous transplantation 13.86; hepatic metastasis, 4.42 6.25; splenic 4.99) were achieved. More importantly, have ability image metastasis less than 1 mm diameter, highlighting potential this be used tool This research may spur use enzyme-activatable probes progress diagnosis image-guided surgery (IGS).

Язык: Английский

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Employing an ICT-FRET Integration Platform for the Real-Time Tracking of SO₂ Metabolism in Cancer Cells and Tumor Models

Journal of the American Chemical Society, Год журнала: 2020, Номер 142(13), С. 6324 - 6331

Опубликована: Март 4, 2020

Glutathione (GSH) mediates a wide variety of biological events and human diseases. Although it has been the subject intense study in recent years, further understanding its molecular mechanisms metabolism routes living cells remained limited due to lack appropriate analytical tools. Sulfur dioxide (SO2), an important metabolite GSH, is usually associated with symptoms neurological disorders, cardiovascular diseases, lung cancer. Herein, novel multisignal fluorescent probe was rationally designed exploited for simultaneous detection GSH SO2 via ICT-FRET synergetic mechanism. The shows completely reversed fluorescence responses toward (enhanced red emission) (annihilated fluorescence) high selectivity sensitivity. In particular, displayed different signals (blue-shift) presence thereby allowing imaging process two independent channels without spectral cross interference. Given these advantages, this successfully applied real-time monitoring metabolic mice models, thus found that can metabolize by enzymatic reaction TST (thiosulfate sulphurtransferase); additionally, transformed into sulfate under SUOX (sulfite oxidase). We anticipate research will provide convenient efficient tool interrelated physiological functions more biosystems.

Язык: Английский

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Heat Stroke in Cell Tissues Related to Sulfur Dioxide Level Is Precisely Monitored by Light-Controlled Fluorescent Probes

Journal of the American Chemical Society, Год журнала: 2020, Номер 142(6), С. 3262 - 3268

Опубликована: Янв. 17, 2020

Heat stroke (HS) can cause serious organism damage or even death. Early understanding of the mechanism heat cytotoxicity prevent treat related diseases. In this work, probe Ly-NT-SP was synthesized, characterized, and used for sulfur dioxide (SO2) detection in lysosomes. PBS solutions at pH 5.0 present a marked broad emission band green zone (535 nm). After UV irradiation, spiropyran group isomerizes to merocyanine form (Ly-NT-MR), which presented weak red-shifted 630 nm. addition, photocontrolled isomerization Ly-NT-MR generated C═C–C═N+ fragment able react, through Michael with SO2 yield highly emissive adduct fluorescence channel vitro studies showed remarkable selectivity photoactivated limit as low 4.7 μM. MTT viability assays demonstrated that is nontoxic HeLa cells be detect Taking advantage this, sensor successfully applied image increasing values lysosomes during shock first time. Moreover, we also confirmed increased protect small intestine against induced by regulating oxidative stress mice.

Язык: Английский

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An Activatable AIEgen Probe for High‐Fidelity Monitoring of Overexpressed Tumor Enzyme Activity and Its Application to Surgical Tumor Excision

Angewandte Chemie International Edition, Год журнала: 2020, Номер 59(25), С. 10186 - 10195

Опубликована: Март 10, 2020

Monitoring fluctuations in enzyme overexpression facilitates early tumor detection and excision. An AIEgen probe (DQM-ALP) for the imaging of alkaline phosphatase (ALP) activity was synthesized. The consists a quinoline-malononitrile (QM) core decorated with hydrophilic phosphate groups as ALP-recognition units. rapid liberation DQM-OH aggregates presence ALP resulted aggregation-induced fluorescence. up-regulation expression cells imaged using DQM-ALP. permeated into 3D cervical liver spheroids spatially heterogeneous high spatial resolution on two-photon microscopy platform, providing fluorescence-guided recognition sub-millimeter tumorigenesis. DQM-ALP enabled differentiation between normal tissue ex vivo vivo, suggesting that may serve powerful tool to assist surgeons during resection.

Язык: Английский

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Chemical Design of Activatable Photoacoustic Probes for Precise Biomedical Applications

Chemical Reviews, Год журнала: 2022, Номер 122(6), С. 6850 - 6918

Опубликована: Март 2, 2022

Photoacoustic (PA) imaging technology, a three-dimensional hybrid modality that integrates the advantage of optical and acoustic imaging, has great application prospects in molecular due to its high depth resolution. To endow PA with ability for real-time visualization precise biomedical diagnosis, numerous activatable probes which can specifically alter their intensities upon reacting targets or biological events interest have been developed. This review highlights recent developments applications including detection biotargets events. First, generation mechanism signals will be given, followed by brief introduction contrast agents used probe design. Then we particularly summarize general design principles alteration strategies developing probes. Furthermore, give detailed discussion living systems. At last, current challenges outlooks future discussed. We hope this stimulate new ideas explore potentials future.

Язык: Английский

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Photoacoustic imaging of elevated glutathione in models of lung cancer for companion diagnostic applications

Nature Chemistry, Год журнала: 2021, Номер 13(12), С. 1248 - 1256

Опубликована: Окт. 25, 2021

Язык: Английский

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Development of NIR-II Photoacoustic Probes Tailored for Deep-Tissue Sensing of Nitric Oxide

Journal of the American Chemical Society, Год журнала: 2021, Номер 143(18), С. 7196 - 7202

Опубликована: Апрель 27, 2021

Photoacoustic (PA) imaging has emerged as a reliable in vivo technique for diverse biomedical applications ranging from disease screening to analyte sensing. Most contemporary PA agents employ NIR-I light (650–900 nm) generate an ultrasound signal; however, there is significant interference endogenous biomolecules such hemoglobin that are active this window. Transitioning longer excitation wavelengths (i.e., NIR-II) reduces the background and facilitates detection of low abundance targets (e.g., nitric oxide, NO). In study, we employed two-phase tuning approach develop APNO-1080, NIR-II NO-responsive probe deep-tissue imaging. First, performed Hammett Brønsted analyses identify highly reactive selective aniline-based trigger reacts with NO via N-nitrosation chemistry. Next, screened panel platforms chemical structures have propensity aggregate since can diminish signal. head-to-head comparison analogue, APNO-1080 was 17.7-fold more sensitive vitro tissue phantom assay. To evaluate capabilities vivo, orthotopic breast cancer model heterotopic lung model. Relative control mice not bearing tumors, normalized turn-on response 1.3 ± 0.12 1.65 0.07, respectively.

Язык: Английский

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Fluorescent Probes for Biological Species and Microenvironments: from Rational Design to Bioimaging Applications

Accounts of Chemical Research, Год журнала: 2023, Номер 56(3), С. 258 - 269

Опубликована: Янв. 18, 2023

Some important biological species and microenvironments maintain a complex delicate dynamic balance in life systems, participating the regulation of various physiological processes playing indispensable roles maintaining healthy development living bodies. Disruption their homeostasis organisms can cause diseases even death. Therefore, real time monitoring these during different pathological is great significance. Fluorescent-probe-based techniques have been recognized as one most powerful tools for imaging samples. In this Account, we introduce representative works from our group field fluorescent probes capable detecting metal ions, small bioactive molecules, microenvironment. The design strategies molecule applications will be discussed. By regulating strategy mechanism (e.g., ICT, PeT, FRET) electronic spectral characteristics platforms, chemical show high selectivity diverse functions, which used processes. Through exploration rational response strategy, combined with variety techniques, such super-resolution imaging, photoacoustic (PA) etc., realized multimode analytes subcellular level to vivo level, provides means study functions microenvironments. This Account aims offer insights inspiration novel could provide biology. Overall, represent series turn-on/turn-off/ratiometric fluorescent/PA visually dynamically trace cells that seek higher resolution depth molecular improve diagnostic methods clarify new discoveries related Our future efforts devoted developing multiorganelle targeted organelle interaction employing dual-mode NIR II PA investigate treat at levels.

Язык: Английский

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A General Approach to Design Dual Ratiometric Fluorescent and Photoacoustic Probes for Quantitatively Visualizing Tumor Hypoxia Levels In Vivo

Angewandte Chemie International Edition, Год журнала: 2021, Номер 61(7)

Опубликована: Июль 6, 2021

Abstract Herein, we describe an energy balance strategy between fluorescence and photoacoustic effects by sulfur substitution to transform existing hemicyanine dyes (Cy) into optimized NIRF/PA dual ratiometric scaffolds. Based on this scaffold, reported the first dual‐ratio response of nitroreductase probe AS‐Cy‐NO 2 , which allows quantitative visualization tumor hypoxia in vivo. composed a new scaffold thioxanthene‐hemicyanine (AS‐Cy‐1) 4‐nitrobenzene moiety, showed 10‐fold NIRF enhancement (I 773 /I 733 ) 2.4‐fold PA (PA 730 /PA 670 upon activation biomarker (nitroreductase, NTR) associated with hypoxia. Moreover, imaging accurately quantified extent high sensitivity depth xenograft breast cancer models. More importantly, 3D maximal intensity projection (MIP) images can precisely differentiate highly heterogeneous oxygen distribution solid tumor. Thus, study provides promising that may be generalized for other disease‐relevant biomarkers.

Язык: Английский

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