Analytical Chemistry,
Год журнала:
2023,
Номер
95(15), С. 6287 - 6294
Опубликована: Апрель 4, 2023
Mitochondrial
membrane
potential
(MMP)
and
sulfur
dioxide
(SO2)
significantly
affect
the
mitochondrial
state.
In
this
work,
TC-2
TC-8
were
constructed
through
side-chain
engineering,
in
which
bearing
poorer
hydrophobicity
could
localize
on
mitochondria
better.
Interestingly,
short-wave
emission
was
captured
due
to
sensitive
response
of
SO2
(LOD
=
13.8
nM).
Meanwhile,
probe
bind
with
DNA,
presenting
enhanced
long-wave
emission.
Encouragingly,
migrate
from
nucleus
when
MMP
decreased,
accompanied
by
increase
fluorescence
lifetime
(9-fold).
Hence,
be
used
for
dual-channel
monitoring
MMP,
showed
a
completely
different
pathway
commercial
detectors
JC-1/JC-10.
The
cellular
experiments
that
gradually
decreased
reactive
oxygen
species-triggered
oxidative
stress,
level
up-regulated
simultaneously.
Overall,
work
proposed
new
method
investigate
diagnose
mitochondrial-related
diseases.
Journal of the American Chemical Society,
Год журнала:
2020,
Номер
142(43), С. 18706 - 18714
Опубликована: Окт. 13, 2020
In
recent
years,
it
has
become
a
trend
to
employ
organic
molecular
fluorescent
probes
with
multireaction
sites
for
the
distinguishable
detection
and
biological
imaging
of
similar
substances.
However,
introduction
brought
great
challenges
synthesis,
at
same
time,
often
destroyed
conjugated
structure
molecules,
leading
an
unsatisfactory
fluorescence
emission
wavelength
not
conducive
practical
application.
As
eternal
theme
life,
metabolism
goes
on
all
time.
Metabolism
is
series
ordered
chemical
reactions
that
occurs
in
organism
maintain
life.
Chemical
can
be
summarized
as
metabolic
pathways.
Simultaneous
monitoring
different
pathways
substance
poses
lofty
challenge
probe.
Here,
we
developed
new
strategy:
construct
through
preliminary
between
some
targets,
which
used
further
distinguish
among
targets
or
detect
their
metabolites,
so
realize
simultaneous
visualization
tracer
multiple
By
intravenous
injection,
revealed
probe
containing
benzopyrylium
ion
target
tumors
efficiently,
thiols
are
highly
expressed
compared
other
tissues
(heart,
lung,
kidney,
liver,
etc.).
The
consumption
by
could
prevent
tumor
growth,
suggesting
cure
was
correlated
thiol
concentration.
construction
reaction
process
novel
idea
pursuit
sites,
will
provide
more
effective
tools
solving
problems.
Chemical Engineering Journal,
Год журнала:
2023,
Номер
464, С. 142553 - 142553
Опубликована: Март 22, 2023
pH,
HSO3−,
and
viscosity
are
crucial
bio-markers
for
evaluating
the
status
of
mitochondria
in
cell
micro-environment.
With
this
research,
we
developed
a
ratiometric
mitochondria-targeting
fluorescent
probe
1
that
was
responsive
toward
bisulphite,
pH
viscosity.
Hydroxylphenyl
1,3-benzoxazole
(D)
acted
as
recognition
unit;
benzo[e]indolium
(A)
rotor
to
achieve
response;
C
=
bond
constructed
by
bridging
D
A
susceptible
nucleophilic
attack
hence
responded
HSO3−.
Probe
exhibits
significant
signal
changes,
high
sensitivity,
outstanding
selectivity,
good
stability
detecting
viscosity,
SO2
solution.
used
imaging
derivatives
live
cells
zebrafish,
indicating
its
potential
identifying
mitochondrial
dysfunctions.
ACS Sensors,
Год журнала:
2024,
Номер
9(2), С. 810 - 819
Опубликована: Янв. 19, 2024
Chronic
wound
healing
is
one
of
the
most
complicated
biological
processes
in
human
life,
which
also
a
serious
challenge
for
health.
During
process,
multiple
pathways
are
activated,
and
various
kinds
reactive
oxygen
species
participate
this
process.
Hydrogen
peroxide
(H2O2)
involves
chronic
wounds
its
concentration
fluctuated
different
pathological
stages
during
Therefore,
H2O2
may
be
recognized
as
powerful
biomarker
to
indicate
However,
roles
cannot
fully
understood
yet.
Herein,
we
proposed
near-infrared
fluorescent
probe
DCM-H2O2
highly
sensitive
rapid
detection
living
cells
scald
incision
mice
models.
exhibited
low
limit
high
specificity
with
cytotoxicity
H2O2,
had
great
potential
application
vivo.
The
was
successfully
utilized
monitor
fluctuation
endogenous
proliferation
process
immortalized
epidermal
(HACAT)
cells,
confirmed
that
participated
cells'
activity
through
growth
factor
signaling
pathway.
In
models,
fluctuations
at
could
obtained
by
vivo
fluorescence
imaging.
Finally,
concentrations
diabetic
foot
tissues
were
probe.
We
expect
