International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(24), С. 17484 - 17484
Опубликована: Дек. 14, 2023
Mesenchymal
stem
cells
(MSCs)
are
a
promising
cell
source
for
therapy
of
intractable
diseases
in
veterinary
medicine,
but
donor-dependent
cellular
heterogeneity
is
an
issue
that
influences
therapeutic
efficacy.
Thus,
we
previously
established
immortalized
maintain
the
fundamental
properties
primary
cells,
functional
evaluation
had
not
been
performed.
Therefore,
evaluated
immunomodulatory
capacity
canine
adipose-derived
MSCs
(cADSCs)
vitro
and
vivo
to
investigate
whether
they
functions.
C57BL/6J
mice
were
treated
with
dextran
sulfate
sodium
(DSS)
induce
colitis,
injected
intraperitoneally
or
cADSCs
on
day
2
DSS
treatment,
observed
10
days.
Administration
improved
body
weight
loss
disease
activity
index
(DAI)
DSS-induced
colitic
by
shifting
peritoneal
macrophage
polarity
from
M1
M2
phenotype,
suppressing
T
helper
(Th)
1/Th17
responses
inducing
regulatory
(Treg)
cells.
They
also
inhibited
proliferation
mouse
vitro.
These
effects
comparable
results
highlight
feasibility
our
as
stable
efficacy
because
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 26, 2024
Advances
in
stem
cell
technology
offer
new
possibilities
for
patients
with
untreated
diseases
and
disorders.
Stem
cell-based
therapy,
which
includes
multipotent
mesenchymal
cells
(MSCs),
has
recently
become
important
regenerative
therapies.
MSCs
are
progenitor
that
possess
the
ability
to
undergo
vitro
self-renewal
differentiate
into
various
lineages.
have
demonstrated
promise
several
areas,
such
as
tissue
regeneration,
immunological
modulation,
anti-inflammatory
qualities,
wound
healing.
Additionally,
development
of
specific
guidelines
quality
control
methods
ultimately
result
therapeutic
application
been
made
easier
by
recent
advancements
study
MSC
biology.
This
review
discusses
latest
clinical
uses
obtained
from
umbilical
cord
(UC),
bone
marrow
(BM),
or
adipose
(AT)
treating
human
pulmonary
dysfunctions,
neurological
disorders,
endocrine/metabolic
diseases,
skin
burns,
cardiovascular
conditions,
reproductive
this
offers
comprehensive
information
regarding
targeted
therapies
utilizing
MSCs.
It
also
presents
examines
concept
origin
its
potential
impact
on
function
downstream
applications.
The
ultimate
aim
research
is
facilitate
translational
applications
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Июль 26, 2024
Mesenchymal
stromal
stem
cells
(MSCs)
possess
a
remarkable
potential
for
numerous
clinical
applications
due
to
their
unique
properties
including
self-renewal,
immunomodulation,
paracrine
actions
and
multilineage
differentiation.
However,
the
translation
of
MSC-based
Advanced
Therapy
Medicinal
Products
(ATMPs)
into
clinic
has
frequently
met
with
inconsistent
outcomes.
One
suspected
reasons
this
issue
is
inherent
extensive
variability
that
exists
among
such
ATMPs,
which
makes
interpretation
efficacy
difficult
assess,
as
well
compare
results
various
studies.
This
stems
from
differences
in
tissue
sources,
donor
attributes,
variances
manufacturing
protocols,
modes
administration.
MSCs
can
be
isolated
tissues
bone
marrow,
umbilical
cord,
adipose
others,
each
its
phenotypic
functional
characteristics.
While
different
sources
do
share
common
features,
they
also
exhibit
distinct
gene
expression
profiles
properites.
Donor-specific
factors
age,
sex,
body
mass
index,
underlying
health
conditions
influence
MSC
phenotype,
morphology,
differentiation
function.
Moreover,
variations
preparation
products
introduces
additional
heterogeneity
result
cell
culture
media
composition,
presence
or
absence
added
growth
factors,
use
serum
supplements
culturing
techniques.
Once
are
formulated,
storage
protocols
play
pivotal
role
efficacy.
Factors
affect
viability
include
concentration,
delivery
solution
importantly,
post-thawing
where
applicable.
Ensuing,
administration
critically
distribution
functionallity
administered
cells.
As
therapies
continue
advance
through
trials,
implication
strategies
reduce
product
imperative.
Central
addressing
these
challenges
need
precise
prediction
responses,
require
well-defined
populations
harmonized
assessment
specific
functions.
By
issues
by
meaningful
approaches,
as,
e.g.,
pooling,
field
overcome
barriers
towards
more
consistent
effective
therapies.
Journal of Translational Medicine,
Год журнала:
2024,
Номер
22(1)
Опубликована: Май 15, 2024
Mesenchymal
stem/stromal
cells
(MSCs)
represent
a
heterogeneous
cell
population
distributed
throughout
various
tissues,
demonstrating
remarkable
adaptability
to
microenvironmental
cues
and
holding
immense
promise
for
disease
treatment.
However,
the
inherent
diversity
within
MSCs
often
leads
variability
in
therapeutic
outcomes,
posing
challenges
clinical
applications.
To
address
this
heterogeneity,
purification
of
MSC
subpopulations
through
marker-based
isolation
has
emerged
as
promising
approach
ensure
consistent
efficacy.
In
review,
we
discussed
reported
markers
MSCs,
encompassing
those
developed
candidate
marker
strategies
high-throughput
approaches,
with
aim
explore
viable
addressing
heterogeneity
illuminate
prospective
research
directions
field.
Journal of Extracellular Vesicles,
Год журнала:
2024,
Номер
13(8)
Опубликована: Авг. 1, 2024
Abstract
Recently,
therapies
utilizing
extracellular
vesicles
(EVs)
derived
from
mesenchymal
stromal/stem
cells
(MSCs)
have
begun
to
show
promise
in
clinical
trials.
However,
EV
therapeutic
potential
varies
with
MSC
tissue
source
and
vitro
expansion
through
passaging.
To
find
the
optimal
for
clinically
translatable
EV‐derived
therapies,
this
study
aims
compare
angiogenic
immunomodulatory
potentials
protein
miRNA
cargo
compositions
of
EVs
isolated
two
most
common
sources
adult
MSCs,
bone
marrow
adipose
tissue,
across
different
passage
numbers.
Primary
marrow‐derived
MSCs
(BMSCs)
adipose‐derived
(ASCs)
were
female
Lewis
rats
expanded
indicated
numbers
(P2,
P4,
P8).
culture
medium
P2,
P8
BMSCs
ASCs
characterized
size,
number,
surface
markers,
content,
morphology.
showed
yields
per
cell,
sizes,
yield
EV.
Gene
ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
pathway
analyses
proteomics
data
seq
identified
key
proteins
pathways
associated
differences
between
BMSC‐EVs
ASC‐EVs,
as
well
due
number.
In
tube
formation
assays
employing
human
umbilical
vein
endothelial
suggested
that
both
number
had
significant
effects
on
capacity
EVs.
With
or
without
lipopolysaccharide
(LPS)
stimulation,
more
significantly
impacted
expression
M2‐macrophage
genes
(IL‐10,
Arg1,
TGFβ)
than
M1‐macrophage
(IL‐6,
NOS2,
TNFα).
By
correlating
analysis
observed
our
immunomodulatory,
angiogenic,
proliferation
assays,
highlights
trade‐offs
may
be
necessary
selecting
development
therapies.
Journal of Xenobiotics,
Год журнала:
2024,
Номер
14(3), С. 827 - 872
Опубликована: Июнь 24, 2024
In
the
recent
past,
formulation
and
development
of
nanocarriers
has
been
elaborated
into
broader
fields
opened
various
avenues
in
their
preclinical
clinical
applications.
particular,
cellular
membrane-based
nanoformulations
have
formulated
to
surpass
surmount
limitations
restrictions
associated
with
naïve
or
free
forms
therapeutic
compounds
circumvent
physicochemical
immunological
barriers
including
but
not
limited
systemic
barriers,
microenvironmental
roadblocks,
other
subcellular
hinderances-which
are
quite
heterogeneous
throughout
diseases
patient
cohorts.
These
drug
delivery
overcome
through
mesenchymal
cells
precision
therapeutics,
where
these
interventions
led
significant
enhancements
efficacies.
However,
still
focuses
on
optimization
paradigms
a
one-size-fits-all
resolutions.
As
stem
cell
engineered
highly
diversified
fashions,
being
optimized
for
delivering
payloads
more
better
personalized
modes,
entering
arena
as
well
nanomedicine.
this
Review,
we
included
some
advanced
which
designed
utilized
both
non-personalized
applicability
can
be
employed
improvements
nanotherapeutics.
present
report,
authors
focused
aspects
advancements
nanoparticle
conceptions
several
roadblocks
It
suggested
that
well-informed
designing
will
lead
appreciable
efficacy
payload
approaches
also
enable
tailored
customized
designs
MSC-based
applications,
finally
amending
outcomes.
World Journal of Stem Cells,
Год журнала:
2025,
Номер
17(1)
Опубликована: Янв. 17, 2025
Human
mesenchymal
stromal
cells
(MSCs)
possess
regenerative
potential
due
to
pluripotency
and
paracrine
functions.
However,
their
stemness
immunomodulatory
capabilities
are
sub-optimal
in
conventional
two-dimensional
(2D)
culture.
To
enhance
the
efficiency
therapeutic
efficacy
of
MSCs,
an
vivo-like
3D
culture
condition
was
applied.
MSCs
were
cultured
on
polystyrene
or
a
gellan
gum-based
system.
In
vitro,
prostaglandin-endoperoxide
synthase
2,
indoleamine-2,3-dioxygenase,
heme
oxygenase
1,
prostaglandin
E
gene
expression
quantified
by
quantitative
real-time
polymerase
chain
reaction.
incubated
with
lipopolysaccharide
(LPS)-treated
mouse
splenocytes,
E2
tumor
necrosis
factor-alpha
levels
measured
enzyme
linked
immunosorbent
assay.
vivo,
LPS
injected
into
lateral
ventricle
brain,
administered
intravenously
next
day.
Animals
sacrificed
analyzed
days
2
6.
Gellan
gum
polymer-based
significantly
increased
octamer-binding
transcription
factor
4
Nanog
homeobox
markers
human
compared
2D
This
environment
also
heightened
cyclooxygenase-2
heme-oxygenase
enzymes
known
for
functions,
including
production
prostaglandins
degradation,
respectively.
secreted
more
effectively
suppressed
release
from
LPS-stimulated
splenocytes
surpassed
2D.
murine
neuroinflammation
model,
intravenous
injection
3D-cultured
reduced
ionized
calcium-binding
adaptor
molecule
1
glial
fibrillary
acidic
protein
expression,
mitigating
chronic
inflammation
than
2D-cultured
MSCs.
The
microenvironment
established
serves
as
vivo
mimetic,
enhancing
function
suggests
that
engineered
hold
significant
promise
potent
tool
cell
therapy.
Disease Models & Mechanisms,
Год журнала:
2025,
Номер
18(2)
Опубликована: Фев. 1, 2025
ABSTRACT
The
transplantation
of
allogeneic
hematopoietic
stem
cells
is
a
potentially
curative
treatment
for
hematological
malignancies,
inherited
blood
disorders
and
immune
deficiencies.
Unfortunately,
30-50%
patients
receiving
will
develop
life-threatening
inflammatory
disease
called
acute
graft-versus-host
(aGVHD).
In
with
aGVHD,
graft-associated
T
cells,
which
typically
target
the
skin,
intestinal
tract
liver,
can
also
damage
lungs
lymphoid
tissue.
Damage
to
tissue
creates
prolonged
immunodeficiency
that
markedly
increases
risk
infections
bleeding,
resulting
in
considerable
morbidity
mortality.
Although
mouse
models
aGVHD
have
been
instrumental
our
understanding
this
condition's
pathogenesis,
translation
preclinical
data
into
new
more
effective
treatments
human
has
limited
reasons
remain
be
fully
understood.
However,
evidence
suggests
factors
associated
likely
contribute
these
unsatisfactory
results.
Review,
we
identify
discuss
specific
inherent
may
limit
patient
treatment,
suggest
how
improve
translatability
models.
