Short-chain fatty acids: linking diet, the microbiome and immunity

Nature reviews. Immunology, Год журнала: 2024, Номер 24(8), С. 577 - 595

Опубликована: Апрель 2, 2024

Язык: Английский

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Review: Chronic endometritis and its effect on reproduction

Journal of Obstetrics and Gynaecology Research, Год журнала: 2019, Номер 45(5), С. 951 - 960

Опубликована: Март 6, 2019

Abstract Aim Chronic endometritis (CE) is a disease of continuous and subtle inflammation characterized by the infiltration plasma cells in endometrial stromal area. Although clinical significance CE has been thought practice for long time because it either asymptomatic or presents with symptoms, recent studies have shown potential adverse effects on fertility. In present review, we focus concept, diagnosis, etiology, pathophysiology, impact reproduction treatment to understand CE. Methods The published articles were reviewed. Results prevalence found be 2.8–56.8% infertile women, 14–67.5% women recurrent implantation failure (RIF), 9.3–67.6% pregnancy loss. Microorganisms are main cause CE, since antibiotic reported an effective therapy Common bacteria frequently detected uterine cavity patients microbial culture. endometrium, immune decidualization modified, these modifications adversely affect gold standard diagnosis histological detection area endometrium specimens, although universally accepted criteria not determined. currently most recovery fertility administration oral antibiotics. Patients whose cured higher ongoing rate, rate compared persistent Conclusion greatly affects impairs Antibiotic therapeutic option. Pregnancy vitro fertilization improved when antibiotic.

Язык: Английский

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Microbial exposure during early human development primes fetal immune cells

Cell, Год журнала: 2021, Номер 184(13), С. 3394 - 3409.e20

Опубликована: Июнь 1, 2021

The human fetal immune system begins to develop early during gestation; however, factors responsible for immune-priming remain elusive. We explored potential exposure microbial agents in utero and their contribution toward activation of memory T cells tissues. profiled microbes across organs using 16S rRNA gene sequencing detected low but consistent signal gut, skin, placenta, lungs the 2nd trimester gestation. identified several live bacterial strains including Staphylococcus Lactobacillus tissues, which induced vitro mesenteric lymph node, supporting role immune-priming. Finally, SEM RNA-ISH, we visualized discrete localization bacteria-like structures eubacterial-RNA within 14th weeks gut lumen. These findings indicate selective presence gestation have broader implications establishment competency priming before birth.

Язык: Английский

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The Endobiota Study: Comparison of Vaginal, Cervical and Gut Microbiota Between Women with Stage 3/4 Endometriosis and Healthy Controls

Scientific Reports, Год журнала: 2019, Номер 9(1)

Опубликована: Фев. 18, 2019

Abstract Dysbiosis in the genital tract or gut microbiome can be associated with endometriosis. We sampled vaginal, cervical and microbiota from 14 women histology proven stage 3/4 endometriosis healthy controls. The V3 V4 regions of 16S rRNA gene were amplified following Metagenomic Sequencing Library Preparation. Despite overall similar intestinal composition between group controls, we observed differences at genus level. complete absence Atopobium vaginal was noteworthy. In microbiota, Gardnerella , Streptococcus Escherichia Shigella Ureoplasma, all which contain potentially pathogenic species increased More had Shigella/Escherichia dominant stool microbiome. Further studies clarify whether association is causal, dysbiosis leads to dysbiosis.

Язык: Английский

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Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Microbiome, Год журнала: 2022, Номер 10(1)

Опубликована: Янв. 4, 2022

Abstract Background Previous evidence indicates associations between the female reproductive tract microbiome composition and outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether endometrial microbiota is associated with outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy. Methods Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing analyse fluid biopsy samples before embryo transfer cohort 342 asymptomatic for infection treatments. Results A dysbiotic profile composed Atopobium , Bifidobacterium Chryseobacterium Gardnerella Haemophilus Klebsiella Neisseria Staphylococcus Streptococcus was unsuccessful outcomes. In contrast, Lactobacillus consistently enriched birth Conclusions Our findings indicate that useful biomarker predict outcome, offering an opportunity further improve diagnosis treatment strategies.

Язык: Английский

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Does the endometrial cavity have a molecular microbial signature?

Scientific Reports, Год журнала: 2019, Номер 9(1)

Опубликована: Июль 9, 2019

Recent molecular studies concluded that the endometrium has a resident microbiota dominated by Lactobacillus spp. and is therefore similar to of vagina. These findings were largely derived from endometrial samples obtained through transcervical catheter thus prone contamination. Herein, we investigated microbial profiles mid-endometrial hysterectomy compared them with those cervix, vagina, rectum, oral cavity, controls for background DNA Microbial examined 16S rRNA gene qPCR sequencing. Universal bacterial total rDNA revealed load exceeding in 60% (15/25) study subjects. Bacterial differed controls, but not cervix. The cervix Acinetobacter, Pseudomonas, Cloacibacterium, Comamonadaceae. Both sequencing species-specific (L. iners & L crispatus) showed was rare endometrium. In conclusion, if there middle endometrium, it as previously concluded, yet further investigation using culture microscopy necessary.

Язык: Английский

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The role of gut and genital microbiota and the estrobolome in endometriosis, infertility and chronic pelvic pain

Human Reproduction Update, Год журнала: 2021, Номер 28(1), С. 92 - 131

Опубликована: Сен. 30, 2021

Endometriosis is a chronic, burdensome condition that historically understudied. Consequently, there lack of understanding the etiology disease and its associated symptoms, including infertility chronic pelvic pain (CPP). development influenced by estrogen metabolism inflammation, which are modulated several factors microbiome estrobolome (the collection genes encoding estrogen-metabolizing enzymes in gut microbiome). Therefore, increasing interest role microbiota endometriosis etiology.To date, no cure for treatment options often ineffective. This manuscript will review potential relationship between endometriosis, CPP highlight available data on relation to related symptoms. The overarching goal this inform future research lead deeper possible diagnostic modalities treatments. impact regulation estrobolome, as well inflammation other endometriosis-promoting mechanisms within genital tract, be reviewed. methodological limitations microbiome-related studies critically assessed provide improved guidelines clinical studies.PubMed databases were searched using following keywords: AND microbiome, IVF (in-vitro fertilization) infertility. Clinical preclinical animal trials eligible review, or included. All manuscripts published 2002-2021.In total, 28 6 included review. In both human studies, bacteria enriched groups, although was clear consensus specific compositions with endometriosis. However, bacterial vaginosis-associated Lactobacillus depletion cervicovaginal majority (23/28) studies. Interpretation endometrial limited owing variety factors, discussed addition, metadata outlining antibiotic usage, age, race/ethnicity, menopausal status timing sample diagnosis not consistently reported. Animal (6/6) support bidirectional onset progression.There evidence dysbiotic multiple gynecologic conditions, mounting supporting an association These microbiomes likely play gut-brain axis, further supports putative spectrum symptoms CPP. Collectively, highlights demand more rigorous transparent methodology controls, consistency across field, inclusion key demographic characteristics comparison participants. Rigorous study designs allow better disorders female reproductive tract.

Язык: Английский

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Uterine Immunity and Microbiota: A Shifting Paradigm

Frontiers in Immunology, Год журнала: 2019, Номер 10

Опубликована: Окт. 17, 2019

The female reproductive tract harbors distinct microbial communities, as in the vagina, cervical canal, uterus, and fallopian tubes. nature of vaginal microbiota is well-known; contrast, upper remains largely unexplored. Alteration uterine microbiota, which dependent on nutrients hormones available to likely play an important role uterine-related diseases such hysteromyoma, adenomyosis, endometriosis. Uterine mucosa tissue barrier whose main function offer protection against pathogens other toxic factors, while maintaining a symbiotic relationship with commensal microbes. These characteristics are shared by all mucosal tissues; however, unique since it changes cyclically during menstrual cycle well pregnancy. immune system, besides its defense process, plays crucial roles reproduction ensures local tolerance fetal/paternal antigens, trophoblast invasion, vascular remodeling. human endometrium contains conspicuous number cells, mainly Natural Killers (NK) phenotypically from peripheral cytotoxic NK, cells macrophages. also few lymphoid aggregates comprising B cell CD8+ T cells. phenotype these change cycle. It has become evident recent years that can be influenced microbiota. Immune sense presence microbes through their pattern recognition receptors, setting up host-microbe interaction. exerts appropriately controlled mechanism competing for space pathogens. recently been considered uterus non-sterile compartment seems possess own There increasing interest characterizing colonization within apparent impact fertility This review will examine potential between present environment.

Язык: Английский

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Pathogenesis of preterm birth: bidirectional inflammation in mother and fetus

Seminars in Immunopathology, Год журнала: 2020, Номер 42(4), С. 413 - 429

Опубликована: Авг. 1, 2020

Abstract Preterm birth (PTB) complicates 5–18% of pregnancies globally and is a leading cause maternal fetal morbidity mortality. Most PTB spontaneous idiopathic, with largely undefined causes. To increase understanding PTB, much research in recent years has focused on using animal models to recapitulate the pathophysiology PTB. Dysfunctions immune adaptations have been implicated range pregnancy pathologies, including A wealth evidence arising from mouse as well human studies now available support that results breakdown fetal-maternal tolerance, along excessive, premature inflammation. In this review, we examine current knowledge bidirectional communication between systems its role immunopathogenesis These insights significantly advance our pathogenesis which essential ultimately designing more effective strategies for early prediction subsequent prevention

Язык: Английский

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Fetal meconium does not have a detectable microbiota before birth

Nature Microbiology, Год журнала: 2021, Номер 6(7), С. 865 - 873

Опубликована: Май 10, 2021

Язык: Английский

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