The future of liver transplantation

Hepatology, Год журнала: 2024, Номер unknown

Опубликована: Март 27, 2024

Over the last 50 years, liver transplantation has evolved into a procedure routinely performed in many countries worldwide. Those able to access this therapy frequently experience miraculous risk-benefit ratio, particularly if they face imminently life-threatening disease. decades, success of transplantation, with dramatic improvements early posttransplant survival, aggressively driven demand. However, despite emergence living donors augment deceased as source organs, supply lagged far behind As result, rationing been an unfortunate focus recent decades. Recent shifts epidemiology disease combined transformative innovations preservation suggest that underlying premise organ shortage may erode foreseeable future. The will sharpen on improving equitable while mitigating constraints related workforce training, infrastructure for recovery and rehabilitation, their associated costs. Research efforts undoubtedly blossom aim optimizing both timing conditions transplantation. Coupled advances genetic engineering, regenerative biology, cellular therapies, portfolio innovation, broad deep, offers promise that, future, not only be broadly available those need but also represent highly durable life-saving therapy.

Язык: Английский

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Research Trends and Dynamics in Single-cell RNA Sequencing for Musculoskeletal Diseases: A Scientometric and Visualization Study

International Journal of Medical Sciences, Год журнала: 2025, Номер 22(3), С. 528 - 550

Опубликована: Янв. 1, 2025

Background: Worldwide, approximately 1.7 billion people are afflicted with musculoskeletal (MSK) diseases, posing significant health challenges. The introduction of single-cell RNA sequencing (scRNA-seq) technology provides novel insights and approaches to comprehend the onset, progression, treatment MSK diseases. Nevertheless, there is a remarkable lack analytical descriptive studies regarding trajectory, essential research directions, current situation, pivotal focuses, upcoming perspectives. Therefore, aim this present comprehensive overview advancements made in scRNA-seq for disorders over past 15 years. Methods: It utilizes robust dataset derived from Web Science Core Collection, encompassing January 1, 2009, through September 6, 2024. To achieve this, advanced methodologies were applied conduct thorough scientometric visual analyses. Results: findings underscore preeminent role China, which contributes 63.49% total publications, thereby exerting substantial impact within domain. Notable contributions came institutions such as Shanghai Jiao Tong University, Sun Yat-sen Harvard Medical School, Liu Yun being leading contributor. Frontiers Immunology published greatest number papers field. This study identified joint bone neoplasms, fractures, intervertebral disc degeneration main focuses. Conclusion: extensive analysis benefits both experienced novice researchers by facilitating immediate access critical data, fostering innovation

Язык: Английский

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Comparative analysis of 10X Chromium vs. BD Rhapsody whole transcriptome single-cell sequencing technologies in complex human tissues

Heliyon, Год журнала: 2024, Номер 10(7), С. e28358 - e28358

Опубликована: Март 19, 2024

The development of single-cell omics tools has enabled scientists to study the tumor microenvironment (TME) in unprecedented detail. However, each different techniques may have its unique strengths and limitations. Here we directly compared two commercially available high-throughput RNA sequencing (scRNA-seq) technologies - droplet-based 10X Chromium

Язык: Английский

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Single-cell transcriptomics reveals heterogeneity in plant responses to the environment: a focus on biotic and abiotic interactions

Journal of Experimental Botany, Год журнала: 2024, Номер 75(17), С. 5188 - 5203

Опубликована: Март 11, 2024

Biotic and abiotic environmental cues are major factors influencing plant growth productivity. Interactions with biotic (e.g. symbionts pathogens) changes in temperature, water, or nutrient availability) trigger signaling downstream transcriptome adjustments plants. While bulk RNA-sequencing technologies have traditionally been used to profile these transcriptional changes, tissue homogenization may mask heterogeneity of responses resulting from the cellular complexity organs. Thus, whether different cell types respond equally fluctuations, subsets cell-type specific, long-lasting questions biology. The recent breakthrough single-cell transcriptomics research offers an unprecedented view under changing conditions. In this review, we discuss contribution understanding cell-type-specific interactions. Besides biological findings, present some technical challenges coupled studies plant-environment interactions, proposing possible solutions exciting paths for future research.

Язык: Английский

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Bile proteome reveals biliary regeneration during normothermic preservation of human donor livers

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Ноя. 30, 2023

Normothermic machine perfusion (NMP) after static cold storage is increasingly used for preservation and assessment of human donor livers prior to transplantation. Biliary viability during NMP reduces the risk post-transplant biliary complications. However, understanding molecular changes in system remains incomplete. We performed an in-depth, unbiased proteomics analysis bile collected sequential hypothermic perfusion, rewarming 55 livers. Longitudinal reveals proteins reflective cellular damage at early stages, followed by upregulation secretory immune response processes. Livers with chemistry acceptable transplantation reveal protein patterns implicated regenerative processes, including proliferation, compared inadequate chemistry. These findings are reinforced detection gene transcripts liver tissue before perfusion. Our comprehensive transcriptomics data sets provide potential further evaluate mechanisms refine criteria.

Язык: Английский

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Abnormal metabolism in hepatic stellate cells: Pandora's box of MAFLD related hepatocellular carcinoma

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Год журнала: 2024, Номер 1879(2), С. 189086 - 189086

Опубликована: Фев. 9, 2024

Язык: Английский

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ormothermic Perfusion of a Human Tumoral Liver for 17 Days with concomitant extracorporeal blood purification therapy: case description

Journal of Hepatology, Год журнала: 2024, Номер 81(3), С. e96 - e98

Опубликована: Май 2, 2024

Язык: Английский

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Single‐cell RNA sequencing reveals reduced intercellular adhesion molecule crosstalk between activated hepatic stellate cells and neutrophils alleviating liver fibrosis in hepatitis B virus transgenic mice post menstrual blood‐derived mesenchymal stem cell transplantation

MedComm, Год журнала: 2024, Номер 5(8)

Опубликована: Июль 22, 2024

Abstract Liver fibrosis can cause hepatitis B virus (HBV)‐associated hepatocellular carcinoma. Menstrual blood‐derived mesenchymal stem cells (MenSCs) ameliorate liver through paracrine. Single‐cell RNA sequencing (scRNA‐seq) may be used to explore the roadmap of activated hepatic stellate cell (aHSC) inactivation target fibrosis. This study established HBV transgenic (HBV‐Tg) mouse model carbon tetrachloride (CCl 4 )‐induced and demonstrated that MenSCs migrated injured improve serological indices reduce fibrotic accumulation. RNA‐bulk analysis revealed mediated extracellular matrix accumulation adhesion. parenchymal nonparenchymal were identified by scRNA‐seq in control, CCl , MenSC groups, revealing heterogeneity fibroblasts/HSCs. A CellChat diminished intercellular adhesion molecule (ICAM) signaling is vital for therapy. Specifically, Icam1 aHSCs acted on Itgal / Itgb2 Itgam neutrophils, causing decreased The expression was higher group than control after therapy neutrophil clusters. Lcn2 Pglyrp1 Wfdc21 Mmp8 had high potential targets neutrophils. highlights interacting cells, corresponding molecules, underlying treating HBV‐associated

Язык: Английский

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Single cell transcriptome profiling of immune tissues from germ-free and specific pathogen-free piglet

Scientific Data, Год журнала: 2025, Номер 12(1)

Опубликована: Апрель 18, 2025

The commensal microbiota provides immunomodulatory signals during the development, differentiation and activation of immune cells, is crucial for maintaining host homeostasis. However, systematic effects on immunity based large animal model at single-cell level remain to be resolved. Here, we utilized RNA sequencing (scRNA-seq) analyze transcriptome profiling containing 57,720 cells from three important tissues [Peyer's patches (PP), mesenteric lymph node (MLN), spleen] germ-free (GF) specific pathogen-free (SPF) piglet. We presented detailed description dataset preliminarily identified major cell types including non-immune further annotated subsets. This a data mining resource researchers involved in microbe-host interactions, enables in-depth analysis map alterations caused by colonization early development. As first transcriptomics tissue GF SPF piglet, this valuable study regulation.

Язык: Английский

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Tissue-resident Lymphocytes Are Released During Hypothermic and Normothermic Machine Perfusion of Human Donor Kidneys

Transplantation, Год журнала: 2024, Номер unknown

Опубликована: Апрель 1, 2024

Background. Machine perfusion is the preferred preservation method for deceased donor kidneys. Perfusate fluid, which contains a complex mixture of components, offers potential insight into organ’s viability and function. This study explored immune cell release, particularly tissue-resident lymphocytes (TRLs), during kidney machine its correlation with injury markers. Methods. samples from hypothermic (HMP; n = 26) normothermic (NMP; 16) human kidneys were analyzed TRLs using flow cytometry. Residency was defined by expressions CD69, CD103, CD49as. TRL release quantified exclusively in NMP. Additionally, levels cell-free DNA, neutrophil gelatinase-associated lipocalin, soluble E-cadherin (sE-cadherin) measured NMP supernatants quantitative polymerase chain reaction enzyme-linked immunosorbent assay. Results. Both HMP contained heterogeneous population TRLs, including CD4 + memory T cells, CD8 natural killer helper-like innate lymphoid cells. Median proportions among total CD45 0.89% (NMP) 0.84% (HMP). quantities did not correlate characteristics, parameters, posttransplant outcomes, or DNA lipocalin concentrations. However, CD103 positively correlated sE-cadherin, ligand integrin. Conclusions. Human both The associated loss their sE-cadherin but general transplant biomarkers.

Язык: Английский

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