Identification of the arl1 locus controlling leaf rolling and its application in maize breeding

Molecular Breeding, Год журнала: 2025, Номер 45(1)

Опубликована: Янв. 1, 2025

Increasing planting density is one of the most important strategies for generating higher maize yields. Moderate leaf rolling decreases mutual shading leaves and increases photosynthesis population hence tolerance high-density planting. Few genes that control in have been identified, however, their applicability breeding programs remains unclear. Here we identified a abaxially rolled leaf1 (arl1) mutant with extreme found size bulliform cells within adaxial blade surface increased arl1 mutant. Bulk segregation analysis mapping an F2 derived from single cross between inbred line Gui18421 normal locus on chromosome 2. Sequential fine-mapping delimited to 233.56-kb genomic interval containing three candidate genes. Sequence alignment 8-bp insertion coding region Zm00001eb082500, which led frame shift causing premature transcription termination Meanwhile, both deep sequencing Sanger showed Zm00001eb082520 was present but absent arl1. A pair near isogenic lines (NILs) carrying allele (NILGui18421) (NIL ) were developed, NIL plants had greater light transmission photosynthetic rate middle lower canopy than did those NILGui18421 under Furthermore, seed setting rate, more kernels per ear, kernel weight ear NILGui18421, grain yield not affected as increased, suggesting can be used genetic improvement tolerance. Taken together, identification evaluation yield-related traits provide excellent target future improvement. The online version contains supplementary material available at 10.1007/s11032-024-01534-0.

Язык: Английский

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Differential Biochemical and Gene Expression responses to UVB radiation in ten Southern Indian rice (Oryza sativa L. indica) varieties

Plant Physiology and Biochemistry, Год журнала: 2025, Номер unknown, С. 109870 - 109870

Опубликована: Апрель 1, 2025

Язык: Английский

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Switch on and off: Phospho‐events in light signaling pathways

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

ABSTRACT Light is a fundamental environmental cue that dynamically orchestrates plant growth and development through spatiotemporally regulated molecular networks. Among these, phosphorylation, key post‐translational modification, plays crucial role in controlling the function, stability, subcellular localization, protein–protein interactions of light signaling components. This review systematically examines phosphorylation‐dependent regulatory events within Arabidopsis cascade, focusing on its mechanisms, downstream functional consequences, crosstalk with other pathways. We underscore pivotal phosphorylation transduction, elucidating how phosphorylation‐decoding framework transduces information into developmental plasticity to modulate plant–environment interactions.

Язык: Английский

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Golden 2-like Transcription Factors Regulate Photosynthesis under UV-B Stress by Regulating the Calvin Cycle

Plants, Год журнала: 2024, Номер 13(13), С. 1856 - 1856

Опубликована: Июль 5, 2024

UV-B stress can affect plant growth at different levels, and although there is a multitude of evidence confirming the effects radiation on photosynthesis, are fewer studies using physiological assays in combination with multi-omics to investigate photosynthesis alpine plants under stressful environments. Golden 2-like (G2-like/GLK) transcription factors (TFs) highly conserved during evolution may be associated abiotic stress. In this paper, we used Handy-PEA Imaging-PAM Maxi detect chlorophyll fluorescence leaves Rhododendron chrysanthum Pall. (R. chrysanthum) after stress, also investigated effect abscisic acid (ABA) We proteomics, widely targeted metabolomics, transcriptomics study changes photosynthesis-related substances The results showed that was able impair donor side photosystem II (PSII), inhibit electron transfer weaken alleviate damage caused by photosynthetic apparatus. Significant G2-like occurred R. differentially expressed genes localized Calvin cycle were strongly correlated members TF family. Multi-omics measurements together revealed TFs influence regulating cycle. This paper provides insights into conducive adoption measures improve increase yield.

Язык: Английский

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Recent advances in UV-B signalling: interaction of proteins with the UVR8 photoreceptor

Journal of Experimental Botany, Год журнала: 2024, Номер 76(3), С. 873 - 881

Опубликована: Март 25, 2024

The UV RESISTANCE LOCUS 8 (UVR8) photoreceptor mediates many plant responses to UV-B and short wavelength UV-A light. UVR8 functions through interactions with other proteins which lead extensive changes in gene expression. Interactions particular determine the nature of response UV-B. It is therefore important understand molecular basis these interactions: how are different able bind differential binding regulated? This concise review highlights recent developments addressing questions. Key advances discussed regard to: identification that interact UVR8; mechanism accumulation nucleus; photoactivation monomer; structural interaction between CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) REPRESSOR OF PHOTOMORPHOGENESIS (RUP) proteins; role phosphorylation modulating Nevertheless, much remains be understood, need extend future research growing list interactors emphasized.

Язык: Английский

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Study of changes in folding/unfolding properties and stability of Arabidopsis thaliana MYB12 transcription factor following UV-B exposure in vitro

Heliyon, Год журнала: 2024, Номер 10(13), С. e34189 - e34189

Опубликована: Июль 1, 2024

Flavonoids mostly protect plant cells from the harmful effects of UV-B radiation sun. In plants, R2R3-subfamily MYB transcription factor, MYB12, is a key inducer biosynthesis flavonoids. Our study involves biophysical characterization Arabidopsis thaliana MYB12 protein (AtMYB12) under exposure in vitro. Tryptophan fluorescence studies using recombinant full-length AtMYB12 (native) and N-terminal truncated versions (first domain absent AtMYB12Δ1, both first second domains AtMYB12Δ2) have revealed prominent alteration tryptophan microenvironment AtMYB12Δ1 AtMYB12Δ2 as result compared with native AtMYB12. Bis-ANS binding assay urea-mediated denaturation profiling showed an appreciable change structural conformation proteins following irradiation. UV-B-treated higher predisposition aggregate formation CD spectral analyses decrease α-helix percentage concomitant increase random coiled structure to treatment. Overall, these findings highlight critical function maintaining stability stress

Язык: Английский

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Chromosome-level baobab genome illuminates its evolutionary trajectory and environmental adaptation

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Окт. 12, 2024

Abstract Baobab ( Adansonia digitata ) is a long-lived tree endemic to Africa with economic, ecological, and cultural importance, yet its genomic features are underexplored. Here, we report chromosome-level reference genome anchored 42 chromosomes for A. , alongside draft assemblies sibling tree, two trees from distinct locations in Africa, za Madagascar. The baobab uniquely rich DNA transposons, which make up 33%, while LTR retrotransposons account 10%. experienced whole multiplication (WGM) around 30 million years ago (MYA), followed by second WGM event 3–11 MYA, likely linked autotetraploidy. Resequencing of 25 identify three subpopulations, gene flow across West East Africa. Gene enrichment fixation index Fst analyses show retained multiple circadian, flowering, light-responsive genes, support longevity through the UV RESISTANCE LOCUS 8 (UVR8) pathway. In sum, provide resources insights breeding conservation.

Язык: Английский

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Chromosome-level baobab (Adansonia digitata) genome illuminates its evolutionary insights

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 16, 2024

Abstract Baobab, Adansonia digitata , is a long-lived tree endemic to Africa that holds great economic, ecological, and cultural value. However, our knowledge of its genomic features, evolutionary history, diversity limited, rendering it orphaned scientifically. We generated haploid chromosome-level reference genome anchored into 42 chromosomes for A. as well draft assemblies sibling tree, two trees from distinct locations in Africa, related species, za Madagascar. Unlike any other plant date, DNA transposable elements (TEs) make up 33% the compared only 10% long terminal repeat retrotransposons (LTR-RTs), which are usually predominant genomes. Baobab has undergone whole duplication (WGD) shared with Malvoideae ∼30 million years ago (MYA), confirmed autotetraplody event 3-4 MYA coincides most recent burst TE insertions. Resequencing 25 revealed three subpopulations suggest gene flow through West but separated East Africa. Gene enrichment analysis baobab-specific high fixation index (Fst) suggested baobab may have retained multiple copies circadian, light growth genes coordinate protection longevity UV RESISTANCE LOCUS 8 ( UVR8 ) synchronizing flower development pollinators. This study lays groundwork creation breeding resources conservation biodiversity.

Язык: Английский

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Chromosome-level baobab (Adansonia digitata) genome illuminates its evolutionary insights

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Апрель 17, 2024

Baobab, (Adansonia digitata), is a long-lived tree endemic to Africa that holds great economic, ecological, and cultural value. However, our knowledge of its genomic features, evolutionary history, diversity limited, rendering it orphaned scientifically. We generated haploid chromosome-level reference genome anchored into 42 chromosomes for A. digitata, as well draft assemblies sibling tree, two trees from distinct locations in Africa, related species, za Madagascar. Unlike any other plant date, DNA transposable elements (TEs) make up 33% the digitata compared only 10% long terminal repeat retrotransposons (LTR-RTs), which are usually predominant genomes. Baobab has undergone whole duplication (WGD) shared with Malvoideae ~30 million years ago (MYA), confirmed autotetraplody event 3-4 MYA coincides most recent burst TE insertions. Resequencing 25 revealed three subpopulations suggest gene flow through West but separated East Africa. Gene enrichment analysis baobab-specific high fixation index (Fst) suggested baobab may have retained multiple copies circadian, light growth genes coordinate protection longevity UV RESISTANCE LOCUS 8 (UVR8) synchronizing flower development pollinators. This study lays groundwork creation breeding resources conservation biodiversity.

Язык: Английский

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Variation in ultraviolet-B (UV-B)-induced DNA damage repair mechanisms in plants and humans: an avenue for developing protection against skin photoaging

International Journal of Radiation Biology, Год журнала: 2024, Номер unknown, С. 1 - 12

Опубликована: Сен. 4, 2024

The increasing amounts of ultraviolet-B (UV-B) light in our surroundings have sparked worries about the possible effects on humans and plants. detrimental heightened UV-B exposure these two vital elements terrestrial life are different due to their unique concurrent nature. Understanding common vulnerabilities distinctive adaptations radiation by exploring physiological biochemical responses plants human health is huge importance. comparative animals, however, poorly studied. This review sheds sophisticated web navigating complex interaction between botanical medical perspectives, drawing upon current findings.

Язык: Английский

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