Electron-donable heterojunctions with synergetic Ru-Cu pair sites for biocatalytic microenvironment modulations in inflammatory mandible defects

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 6, 2024

Abstract The clinical treatments of maxillofacial bone defects pose significant challenges due to complex microenvironments, including severe inflammation, high levels reactive oxygen species (ROS), and potential bacterial infection. Herein, we propose the de novo design an efficient, versatile, precise electron-donable heterojunction with synergetic Ru-Cu pair sites (Ru-Cu/EDHJ) for superior biocatalytic regeneration inflammatory mandible pH-controlled antibacterial therapies. Our studies demonstrate that unique structure Ru-Cu/EDHJ enhances electron density Ru atoms optimizes binding strength species, thus improving enzyme-like catalytic performance. Strikingly, this biocompatible can efficiently switch between ROS scavenging in neutral media generation acidic media, simultaneously exhibiting repair functions bioadaptive properties treating male mice. We believe synthesizing such heterojunctions exceptional capabilities will offer a promising pathway engineering materials treat trauma, tumors, or infection-caused defects.

Язык: Английский

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Diverse Functions of Macrophages in Obesity and Metabolic Dysfunction-Associated Steatotic Liver Disease: Bridging Inflammation and Metabolism

Immune Network, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 1, 2025

Macrophages play crucial roles in immune response and tissue homeostasis, with their functions becoming increasingly complex obesity-mediated metabolic disorders. This review explores the extensive range of macrophage activities within adipose liver tissues, emphasizing contribution to pathogenesis progression obesity its related dysfunction-associated steatotic disease (MASLD). In context obesity, macrophages respond adaptively lipid overloads inflammatory cues tissue, profoundly influencing insulin resistance homeostasis. Concurrently, role extends moderating inflammation orchestrating fibrotic responses, integral development MASLD. Highlighting spectrum phenotypes across these landscapes, we summarize diverse linking processes functions. advocates for a deeper understanding subsets proposing targeted research harness therapeutic potential mitigating MASLD other

Язык: Английский

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Tissue-Specific Metabolic Reprogramming in Innate Lymphoid Cells and Its Impact on Disease

Immune Network, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 1, 2025

Recent advances have highlighted the crucial role of metabolic reprogramming in shaping functions innate lymphoid cells (ILCs), which are vital for tissue immunity and homeostasis. As tissue-resident cells, ILCs dynamically respond to local environmental cues, with tissue-derived metabolites such as short-chain fatty acids amino directly modulating their effector functions. The states ILC subsets-ILC1, ILC2, ILC3-are closely linked ability produce cytokines, sustain survival, drive proliferation. This review provides a comprehensive analysis how key pathways, including glycolysis, oxidative phosphorylation, acid oxidation, influence activation function. Furthermore, we explore complex interactions between these pathways tissue-specific metabolites, can shape ILC-mediated immune responses health disease. Understanding reveals new insights into pathogenesis conditions asthma, inflammatory bowel disease, cancer. A deeper understanding mechanisms may not only advance our knowledge disease but also lead development novel therapeutic strategies targeting treat disorders.

Язык: Английский

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M2 macrophage-derived TGF-β induces age-associated loss of adipogenesis through progenitor cell senescence

Molecular Metabolism, Год журнала: 2024, Номер 84, С. 101943 - 101943

Опубликована: Апрель 23, 2024

Adipose tissue is an endocrine and energy storage organ composed of several different cell types, including mature adipocytes, stromal cells, endothelial a variety immune cells. aging contributes to the pathogenesis metabolic dysfunction likely induced by crosstalk between adipose progenitor cells (APCs) but underlying molecular mechanisms remain largely unknown. In this study, we revealed biological role p16high senescent APCs, investigated each type in aged white tissue. We performed single-cell RNA sequencing (scRNA-seq) analysis on sorted from p16-CreERT2/Rosa26-LSL-tdTomato mice. also time serial age-dependent bulk RNA-seq datasets human mouse tissues infer transcriptome alteration adipogenic potential within aging. show that M2 macrophage-derived TGF-β induces APCs senescence which impairs adipogenesis vivo. increase with age loss potential. The ligand-receptor interaction reveals macrophages are donors for recipients. Indeed, treatment TGF-β1 phenotypes through mitochondrial ROS-mediated DNA damage vitro. injection into gonadal (gWAT) suppresses fibrotic genes as well p16 APCs. A gWAT atrophy observed cancer cachexia senescence, whose induction appeared be independent induction. Our results suggest age-related lipodystrophy senescence. damage, ROS, finally cellular

Язык: Английский

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Transcriptional control of metabolism by interferon regulatory factors

Nature Reviews Endocrinology, Год журнала: 2024, Номер 20(10), С. 573 - 587

Опубликована: Май 20, 2024

Язык: Английский

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Immune Regulatory Crosstalk in Adipose Tissue Thermogenesis

Comprehensive physiology, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 1, 2025

Brown adipose tissue (BAT) and thermogenic beige fat within white (WAT), collectively known as adaptive fat, dissipate energy heat, offering promising therapeutic potential to combat obesity metabolic disorders. The specific biological functions of these depots are determined by their unique interaction with the microenvironments, composed immune cells, endothelial pericytes, nerve fibers. Immune cells residing in play a key role regulating expenditure systemic homeostasis. dynamic microenvironment is essential for maintaining health function. infiltrate both BAT WAT, contributing homeostasis activation through intricate cellular communications. Emerging evidence underscores importance various cell populations tissue, though many remain undercharacterized. This review provides comprehensive overview that regulate thermogenesis complex interactions niche, highlighting influence contribute interventions syndrome.

Язык: Английский

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Tirzepatide reduces body weight by increasing fat utilization via the central nervous system‐adipose tissue axis in male mice

Diabetes Obesity and Metabolism, Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

Tirzepatide, a dual glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) receptor agonist, demonstrates promise as potent medication for obesity. However, the extent to which its weight-reducing effects go beyond suppressing appetite remains unclear. This study aimed elucidate this by establishing pair-fed control group, effectively eliminating influence of reduced caloric intake. Mice fed on chow diet or high-fat received single long-term intracerebroventricular (i.c.v.) injections tirzepatide vehicle. The vehicle-treated mice were tirzepatide-treated group avoid impact induced different intakes. Body weight food intake monitored daily. Respiratory exchange ratio (RER) was determined in metabolic cages. Fat utilization calculated based RER. Parameters lipid metabolism evaluated. receiving i.c.v. administration exhibited significant reductions body fat content compared with controls. These mediated increased lipolytic capacity white adipose tissue enhanced thermogenesis brown beige tissues, leading decreased RER utilization. Mechanistic investigations revealed that these primarily sympathetic nervous system innervation tissues. innervation, turn, might be associated neuronal activity changes dorsomedial hypothalamus nucleus solitary tract within hindbrain. findings establish critical role shifting substrate preference through central system-adipose axis, promoting loss independent

Язык: Английский

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Jatrorrhizine retard obesity by modulating transcription factor c-Jun/c-Fos to downregulate Mmp12-mediated inflammation

International Immunopharmacology, Год журнала: 2025, Номер 152, С. 114405 - 114405

Опубликована: Март 13, 2025

Язык: Английский

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Therapeutic potential of adipose-derived stem cell extracellular vesicles: from inflammation regulation to tissue repair

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 7, 2024

Inflammation is a key pathological feature of many diseases, disrupting normal tissue structure and resulting in irreversible damage. Despite the need for effective inflammation control, current treatments, including stem cell therapies, remain insufficient. Recently, extracellular vesicles secreted by adipose-derived cells (ADSC-EVs) have garnered attention their significant anti-inflammatory properties. As carriers bioactive substances, these demonstrated potent capabilities modulating promoting repair conditions such as rheumatoid arthritis, osteoarthritis, diabetes, cardiovascular stroke, wound healing. Consequently, ADSC-EVs are emerging promising alternatives to conventional ADSC-based offering advantages reduced risk immune rejection, enhanced stability, ease storage handling. However, specific mechanisms which regulate under not fully understood. This review discusses role impact on disease prognosis, potential promote repair. Additionally, it provides insights into future clinical research focused ADSC-EV therapies inflammatory overcome some limitations associated with cell-based therapies.

Язык: Английский

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Revisited guidelines for metabolic tolerance tests in mice

Lab Animal, Год журнала: 2024, Номер 54(1), С. 16 - 23

Опубликована: Ноя. 25, 2024

Abstract Preclinical mouse models are extensively used in biomedical research to gain insight into disease mechanisms and test new drug treatments. Glucose insulin tolerance tests simple experimental frequently worldwide assess glucose metabolism mice. Various guidelines methodological considerations have been published help researchers standardize procedures optimize outcomes. Yet, there is still important heterogeneity the way these performed, with no real consensus on what best practices achieve high-quality reproducible results. Here we critically examine several recent technical reports how perform metabolic laboratory mice discuss influence of various confounding factors We hope this work will scientists establish more consensual for maximizing relevance clinical translation studies using research.

Язык: Английский

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