Deleted Journal,
Год журнала:
2025,
Номер
70(1), С. 4 - 4
Опубликована: Фев. 5, 2025
Microbial
succession
on
urban
masonry
surfaces
is
a
critical
yet
understudied
aspect
of
environmental
microbiology,
with
implications
for
public
health
within
the
One
Health
framework.
This
study
investigated
how
building
age,
orientation,
and
vegetative
cover
influence
microbial
diversity
masonry,
metal,
glass
substrates
at
University
La
Verne,
California.
Biophysical
characterizations
were
conducted,
communities
analyzed
using
morphospecies
richness
DNA
sequencing.
Significant
variation
in
species
(χ2
=
20.3882,
p
0.0011)
(Fisher’s
LSD,
<
0.05)
was
observed.
Masonry
exhibited
highest
diversity,
mean
23
compared
to
14
metal
12
glass.
Penicillium
fungi
dominated
sites,
comprising
45%
colonies,
while
Pseudomonas
Klebsiella
more
prevalent
(35%
28%,
respectively).
did
not
follow
linear
progression
but
showed
non-sequential
shifts
influenced
by
local
conditions.
The
abundance
Penicillium,
known
antibiotic
production,
raises
concerns
about
spreading
resistance
genes.
Opportunistic
pathogens
further
highlight
potential
risks.
These
findings
underscore
importance
understanding
primary
successional
processes
environments
manage
mitigate
Vaccine,
Год журнала:
2024,
Номер
42(19), С. S125 - S141
Опубликована: Март 19, 2024
Klebsiella
pneumoniae
causes
community-
and
healthcare-associated
infections
in
children
adults.
Globally
2019,
an
estimated
1.27
million
(95%
Uncertainty
Interval
[UI]:
0.91-1.71)
4.95
UI:
3.62-6.57)
deaths
were
attributed
to
associated
with
bacterial
antimicrobial
resistance
(AMR),
respectively.
K.
was
the
second
leading
pathogen
AMR
resistant
bacteria.
Furthermore,
rise
of
both
hospital-acquired
is
a
concern
for
neonates
infants
who
are
at
high
risk
invasive
disease.
There
limited
antibiotic
pipeline
new
antibiotics
treat
multidrug
infections,
vaccines
targeted
against
considered
be
priority
by
World
Health
Organization.
Vaccination
pregnant
women
could
reduce
K.pneumoniae
disease
their
young
offspring.
In
addition,
vulnerable
children,
adolescents
adult
populations
underlying
diseases
such
as
immunosuppression
from
hematologic
malignancy,
chemotherapy,
patients
undergoing
abdominal
and/or
urinary
surgical
procedures,
or
prolonged
intensive
care
management
also
potential
target
groups
vaccine.
A
'Vaccine
Value
Profile'
(VVP)
K.pneumoniae,
which
contemplates
vaccination
protect
babies
birth
through
least
three
months
age
other
high-risk
populations,
provides
high-level,
holistic
assessment
available
information
inform
public
health,
economic
societal
value
preventatives
therapeutics.
This
VVP
developed
working
group
subject
matter
experts
academia,
non-profit
organizations,
public-private
partnerships,
multi-lateral
collaboration
stakeholders
WHO.
All
contributors
have
extensive
expertise
on
various
elements
collectively
aimed
identify
current
research
knowledge
gaps.
The
using
only
existing
publicly
information.
The
hypervirulent
carbapenem-resistant
Klebsiella
pneumoniae
(hv-CRKP)
poses
a
substantial
challenge
to
the
global
health
care.
However,
mechanism
behind
its
evolution
and
transmission
remain
elusive.
Here,
four
virulence
plasmid
types
were
identified
from
310
hv-CRKP
isolates
collected
nationwide
during
2017–2018,
based
on
their
aerobactin
(
iuc
locus)
lineage
IncFIB
replicons.
Notably,
pIUC1-IncFIB(K)
37
pIUC1-IncFIB(Mar),
representing
two
epidemic
plasmids
in
Asia
Europe,
respectively,
accounted
for
>90%
of
episodes.
Analysis
494
K.
(376
2010–2013;
118
2017–2018)
2578
public
genomes
indicated
notable
role
IncFIB(Mar)
emergence
spread.
Conjugation
assays
showed
helper
could
efficiently
transfer
into
strain
uniquely
retromobilize
with
back
CRKP.
Thereafter,
either
lost
rapidly
or
recombined
,
generating
hybrid
pIUC1-IncFIB(Mar)
plasmid.
Our
findings
elucidated
formation,
evolution,
dissemination
trajectories
major
strains
different
regions.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Март 6, 2024
Abstract
Bacterial
evolution
is
affected
by
mobile
genetic
elements
like
phages
and
conjugative
plasmids,
offering
new
adaptive
traits
while
incurring
fitness
costs.
Their
infection
the
bacterial
capsule.
Yet,
its
importance
has
been
difficult
to
quantify
because
of
high
diversity
confounding
mechanisms
in
genomes
such
as
anti-viral
systems
surface
receptor
modifications.
Swapping
capsule
loci
between
Klebsiella
pneumoniae
strains
allowed
us
their
impact
on
plasmid
phage
independently
background.
Capsule
swaps
systematically
invert
susceptibility,
revealing
serotypes
key
determinants
infection.
types
also
influence
conjugation
efficiency
both
donor
recipient
cells,
a
mechanism
shaped
volume
pilus
structure.
Comparative
genomics
confirmed
that
more
permissive
lab
correspond
acquiring
plasmids
nature.
The
least
capsule-sensitive
pili
(F-like)
are
most
frequent
species’
only
ones
associated
with
antibiotic
resistance
virulence
factors,
driving
convergence
antibiotics
population.
These
results
show
how
cellular
envelopes
define
slow
fast
lanes
elements,
implications
for
population
dynamics
horizontal
gene
transfer.
Nature Communications,
Год журнала:
2022,
Номер
13(1)
Опубликована: Дек. 1, 2022
Abstract
Opportunistic
bacterial
pathogen
species
and
their
strains
that
colonise
the
human
gut
are
generally
understood
to
compete
against
both
each
other
commensal
colonising
this
ecosystem.
Currently
we
lacking
a
population-wide
quantification
of
strain-level
colonisation
dynamics
relationship
potential
prevalence
in
disease,
how
ecological
factors
might
be
modulating
these.
Here,
using
combination
latest
high-resolution
metagenomics
genomic
epidemiology
methods
performed
characterisation
competition
for
longitudinal
cohort
neonatal
microbiomes.
We
found
strong
inter-
intra-species
process,
but
also
number
synergistic
relationships
among
several
belonging
genus
Klebsiella
,
which
includes
prominent
pneumoniae
.
No
evidence
preferential
by
hospital-adapted
lineages
either
vaginal
or
caesarean
section
birth
groups
was
detected.
Our
analysis
further
enabled
unbiased
assessment
extra-intestinal
pathogenic
Escherichia
coli
(ExPEC)
comparison
with
propensity
cause
bloodstream
infections.
study
highlights
importance
systematic
surveillance
pathogens,
not
only
from
disease
carriage
state,
better
inform
therapies
preventive
medicine
future.
The Lancet Microbe,
Год журнала:
2023,
Номер
4(11), С. e943 - e952
Опубликована: Окт. 16, 2023
One
Health
approaches
to
address
the
increasing
threat
of
antimicrobial
resistance
(AMR)
are
gaining
attention.
However,
data
on
distribution
and
movement
bacteria
their
AMR-associated
genes
between
clinical
non-clinical
sources
scarce,
especially
from
low-income
middle-income
countries.
We
aimed
analyse
Klebsiella
isolates
various
in
Ghana
compare
prevalence
AMR
with
datasets
two
other
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Июнь 20, 2023
Abstract
The
intrinsic
virulence
of
extra-intestinal
pathogenic
Escherichia
coli
is
associated
with
numerous
chromosomal
and/or
plasmid-borne
genes,
encoding
diverse
functions
such
as
adhesins,
toxins,
and
iron
capture
systems.
However,
the
respective
contribution
to
those
genes
seems
depend
on
genetic
background
poorly
understood.
Here,
we
analyze
genomes
232
strains
sequence
type
complex
STc58
show
that
(quantified
in
a
mouse
model
sepsis)
emerged
sub-group
due
presence
siderophore-encoding
high-pathogenicity
island
(HPI).
When
extending
our
genome-wide
association
study
370
strains,
full
aer
or
sit
operons,
addition
HPI.
prevalence
these
their
co-occurrence
genomic
location
strain
phylogeny.
Thus,
selection
lineage-dependent
specific
associations
virulence-associated
argues
for
strong
epistatic
interactions
shaping
emergence
E.
.
