Integrating 3D Bioprinting and Organoids to Better Recapitulate the Complexity of Cellular Microenvironments for Tissue Engineering

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Дек. 8, 2024

Abstract Organoids, with their capacity to mimic the structures and functions of human organs, have gained significant attention for simulating pathophysiology been extensively investigated in recent past. Additionally, 3D bioprinting, as an emerging bio‐additive manufacturing technology, offers potential constructing heterogeneous cellular microenvironments, thereby promoting advancements organoid research. In this review, latest developments bioprinting technologies aimed at enhancing engineering are introduced. The commonly used methods materials organoids, a particular emphasis on advantages combining organoids summarized. These include achieving high cell concentrations form large aggregates, precise deposition building blocks create complex functions, automation throughput ensure reproducibility standardization culture. Furthermore, review provides overview relevant studies from years discusses current limitations prospects future development.

Язык: Английский

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Organs-on-chip technology: a tool to tackle genetic kidney diseases

Pediatric Nephrology, Год журнала: 2022, Номер 37(12), С. 2985 - 2996

Опубликована: Март 14, 2022

Abstract Chronic kidney disease (CKD) is a major healthcare burden that takes toll on the quality of life many patients. Emerging evidence indicates substantial proportion these patients carry genetic defect contributes to their disease. Any effort reduce percentage with diagnosis nephropathy heading towards replacement therapies should therefore be encouraged. Besides early screenings and registries, in vitro systems mimic complexity pathophysiological aspects could advance screening for targeted personalized therapies. In this regard, use patient-derived cell lines, as well generation disease-specific lines via gene editing stem technologies, have significantly improved our understanding molecular mechanisms underlying inherited diseases. Furthermore, organs-on-chip technology holds great potential it can emulate tissue organ functions are not found other, more simple, models. The nature chips, together physiologically relevant read-outs, provide new opportunities patient-specific assessment, strategies treatment. review, we summarize kidney-on-chip (KOC) configurations present most recent studies representation diseases using KOC-driven strategies.

Язык: Английский

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Application of microfluidic chips in the simulation of the urinary system microenvironment

Materials Today Bio, Год журнала: 2023, Номер 19, С. 100553 - 100553

Опубликована: Янв. 20, 2023

The urinary system, comprising the kidneys, ureters, bladder, and urethra, has a unique mechanical fluid microenvironment, which is essential to system growth development. Microfluidic models, based on micromachining tissue engineering technology, can integrate pathophysiological characteristics, maintain cell-cell cell-extracellular matrix interactions, accurately simulate vital characteristics of human microenvironments. Additionally, these models facilitate improved visualization integration meet requirements laminar flow environment system. However, several challenges continue impede development microenvironment with controllable conditions closely resemble physiological conditions. In this review, we describe biochemical physical explore feasibility microfluidic technology in simulating

Язык: Английский

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Controllable Histotomy Based on Hierarchical Magnetic Microneedle Array Robots

Engineering, Год журнала: 2024, Номер 42, С. 166 - 174

Опубликована: Май 21, 2024

Investigation of patient-derived primary tissues is great importance in the biomedical field, but recent tissue slicing and cultivation techniques still have difficulties satisfying clinical requirements. Here, we propose a controllable histotomy strategy that utilizes hierarchical magnetic microneedle array robots to tailor establish desired high-throughput tissue-on-a-chip. This was performed using three-dimensional printed, mortise-tenon-structured device coupled with magnetic-particle-loaded pagoda-shaped scaffold. Due multilayered structure these microneedles, specimens can be fixed onto scaffold via mechanical interlocking, thereby effectively avoiding slipping during process. Owing encapsulation fragments, pieces act as magnetically responsive biohybrid microrobots easily manipulated by fields, facilitating their separation, transportation, dynamic culture. Using this strategy, demonstrated pancreatic cancer tailored into tiny cultured microfluidic chips for efficient drug screening, indicating promising future technique's application settings.

Язык: Английский

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Microfluidic organoid-slice-on-a-chip system for studying anti-cholangiocarcinoma drug efficacy and hepatorenal toxicity

Lab on a Chip, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Organ-chip technology, in contrast to cell culture and animal models, offers a promising platform for accelerating drug development. However, current chip designs simulate human organ functions there is lack of multi-organ that can simultaneously study efficacy hepatorenal toxicity. Here, we developed novel microfluidic integrated cholangiocarcinoma organoids (CCOs) with recellularized liver slices (RLS) kidney (RKS), assess anti-cholangiocarcinoma Co-culture patient-derived CCOs RLS RKS was successfully achieved 7 days under flow conditions enhanced renal functions. Furthermore, an vitro biomimetic model showed IC50 values trastuzumab emtansine (T-DM1) around 6.42 ± 7.34 μg mL-1 four clinical cases, one outlier 77.77 due patient variability. Post-treatment, viability remained high at 75.67% 81.03%, respectively, suggesting low toxicity T-DM1 treating cholangiocarcinoma. Our demonstrates the use organoid-slice-on-a-chip (OSOC) personalized assessment, particularly aiming leveraging anticancer drugs off-label save lives.

Язык: Английский

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Hemodynamic simulation and in vitro modeling of three-dimensional glomeruli at anatomical scale

Physics of Fluids, Год журнала: 2025, Номер 37(5)

Опубликована: Май 1, 2025

The glomerulus is a critical filtration unit in the kidney, yet its complex three-dimensional architecture has long hindered comprehensive understanding of function and regulation. Here, we present an integrated framework that combines vivo imaging based modeling, computational fluid dynamics simulations, vitro reconstruction to elucidate structural hemodynamic complexity glomerulus. Our analyses reveal inherent asymmetry between afferent efferent arterioles for establishing precise pressure-flow relationship regulating hemodynamics. We further successfully fabricated perfusable, anatomically accurate mouse within microphysiological system, demonstrating proof-of-concept perfusion analysis vascularization. These findings establish transformative platform studying glomerular diseases pave way therapeutic interventions.

Язык: Английский

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Advancements in organs-on-chips technology for viral disease and anti-viral research

Organs-on-a-Chip, Год журнала: 2023, Номер 5, С. 100030 - 100030

Опубликована: Июнь 1, 2023

Disease models that can accurately recapitulate human pathophysiology during infection and clinical response to antiviral therapeutics are still lacking, which represents a major barrier in drug development. The emergence of Organs-on-a-Chip integrated microfluidics with three-dimensional (3D) cell culture, may become the potential solution for this urgent need. Human aims by incorporating tissue-relevant types their microenvironment, such as dynamic fluid flow, mechanical cues, tissue–tissue interfaces, immune cells increase predictive validity vitro experimental models. has broad range applications basic biomedical research, preclinical development, personalized medicine. This review focuses on its use fields virology infectious diseases. We reviewed various Organs-on-a-Chip-based viral application studying life cycle, pathogenesis, virus-host interaction, responses virus- host-targeted therapies. conclude proposing challenges future research avenues leveraging promising technology prepare pandemics.

Язык: Английский

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Cortical Organoid-on-a-Chip with Physiological Hypoxia for Investigating Tanshinone IIA-Induced Neural Differentiation

Research, Год журнала: 2023, Номер 6

Опубликована: Янв. 1, 2023

Cortical organoids represent cutting-edge models for mimic human brain development during the early and even middle stage of pregnancy, while they often fail to recreate complex microenvironmental factors, such as physiological hypoxia. Herein, recapitulate fetal development, we propose a novel cortical organoid-on-a-chip with hypoxia further explore effects tanshinone IIA (Tan IIA) in neural differentiation. The microfluidic chip was designed micropillar array controlled efficient generation organoids. With low oxygen, generated could key aspects early-gestational development. Compared normoxic culturing condition, promoted neurogenesis, synaptogenesis neuronal maturation were observed present microsystem, suggesting significance Based on this model, have found that Chinese herbal drug Tan promote differentiation maturation, indicating its potential therapeutic neurodevelopmental disorders well congenital neuropsychiatric diseases. These results indicate proposed biomimetic model can offer promising platform simulate prenatal environment, screen natural neuroactive components.

Язык: Английский

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3D-Printing of succulent plant-like scaffolds with beneficial cell microenvironments for bone regeneration

Journal of Materials Chemistry B, Год журнала: 2023, Номер 11(24), С. 5523 - 5536

Опубликована: Янв. 1, 2023

The succulent plant-like bioceramic scaffolds inspired by the macroscopic structure of plants could improve cellular crosstalk and enhance bone regeneration.

Язык: Английский

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Modeling Podocyte Ontogeny and Podocytopathies with the Zebrafish

Journal of Developmental Biology, Год журнала: 2023, Номер 11(1), С. 9 - 9

Опубликована: Фев. 20, 2023

Podocytes are exquisitely fashioned kidney cells that serve an essential role in the process of blood filtration. Congenital malformation or damage to podocytes has dire consequences and initiates a cascade pathological changes leading renal disease states known as podocytopathies. In addition, animal models have been integral discovering molecular pathways direct development podocytes. this review, we explore how researchers used zebrafish illuminate new insights about processes podocyte ontogeny, model podocytopathies, create opportunities discover future therapies.

Язык: Английский

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