Co‐Electrospun Silk Fibroin and Gelatin Methacryloyl Sheet Seeded with Mesenchymal Stem Cells for Tendon Regeneration

Small, Год журнала: 2022, Номер 18(21)

Опубликована: Апрель 29, 2022

Abstract Silk fibroin (SF) is a promising biomaterial for tendon repair, but its relatively rigid mechanical properties and low cell affinity have limited application in regenerative medicine. Meanwhile, gelatin‐based polymers advantages attachment tissue remodeling insufficient strength to regenerate tough such as tendons. Taking these aspects into account, this study, gelatin methacryloyl (GelMA) combined with SF create mechanically strong bioactive nanofibrous scaffold (SG). The of SG nanofibers can be flexibly modulated by varying the ratio GelMA. Compared nanofibers, mesenchymal stem cells (MSCs) seeded on fibers optimal composition (SG7) exhibit enhanced growth, proliferation, vascular endothelial growth factor production, tenogenic gene expression behavior. Conditioned media from MSCs cultured SG7 scaffolds greatly promote migration proliferation tenocytes. Histological analysis tenogenesis‐related immunofluorescence staining indicate demonstrate vivo regeneration compared other groups. Therefore, rational combinations GelMA hybrid may help improve therapeutic outcomes address challenges tissue‐engineered regeneration.

Язык: Английский

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TMT-Based Quantitative Proteomics Analysis of Synovial Fluid-Derived Exosomes in Inflammatory Arthritis

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Март 11, 2022

To compare the proteomics of synovial fluid (SF)-derived exosomes in rheumatoid arthritis (RA), axial spondyloarthritis (axSpA), gout, and osteoarthritis (OA) patients.Exosomes were separated from SF by Exoquick kit combined ultracentrifugation method. Tandem mass tags (TMT)-labeled liquid chromatography spectrometry (LC-MS/MS) technology was used to analyze SF-derived exosomes. Volcano plot, hierarchical cluster, gene ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis conducted.A total 1,678 credible proteins detected. Sixty-nine differentially expressed found compared with OA, axSpA, RA simultaneously. Twenty-five highly gout uniquely, lysozyme C protein S100-A9 included, whose bioinformatic significantly involved "neutrophil degranulation" "prion diseases". Eighty-four Thirty-nine axSpA RNA-binding 8A transport Sec24C "acute-phase response" "citrate cycle". One hundred eighty-four RA, Twenty-eight pregnancy zone (PZP) stromelysin-1 "serine-type endopeptidase inhibitor activity" "complement coagulation cascades". Enzyme-linked immunosorbent assay (ELISA) result showed that exosome-derived PZP level higher than OA (p < 0.05).Our study for first time described profiles patients. Some potential biomarkers hypothetical molecular mechanisms proposed, which may provide helpful diagnostic therapeutic insights inflammatory (IA).

Язык: Английский

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Exercise for Osteoarthritis: A Literature Review of Pathology and Mechanism

Frontiers in Aging Neuroscience, Год журнала: 2022, Номер 14

Опубликована: Май 3, 2022

Osteoarthritis (OA) has a very high incidence worldwide and become common joint disease in the elderly. Currently, treatment methods for OA include surgery, drug therapy, exercise therapy. In recent years, of certain diseases by received increasing research attention. Proper can improve physiological function various organs body. At present, is usually symptomatic. Limited are available according to its pathogenesis, effective intervention not been developed slow down progress from molecular level. Only clarifying mechanism influence different intensities on patients we choose appropriate prescription prevent treat OA. This review mainly expounds that alleviates pathological changes affecting degradation ECM, apoptosis, inflammatory response, autophagy, ncRNA, summarizes effects types patients. Finally, it found types, intensity, time frequency have same time, suitable prescriptions recommended

Язык: Английский

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A textile magnetoelastic patch for self-powered personalized muscle physiotherapy

Matter, Год журнала: 2023, Номер 6(7), С. 2235 - 2247

Опубликована: Июнь 27, 2023

Язык: Английский

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Effect of Aging on Tendon Biology, Biomechanics and Implications for Treatment Approaches

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(20), С. 15183 - 15183

Опубликована: Окт. 14, 2023

Tendon aging is associated with an increasing prevalence of tendon injuries and/or chronic diseases, such as tendinopathy, which affects approximately 25% the adult population. Aged tendons are often characterized by a reduction in number and functionality stem/progenitor cells (TSPCs), fragmented or disorganized collagen bundles, increased deposition glycosaminoglycans (GAGs), leading to pain, inflammation, impaired mobility. Although exact pathology unknown, overuse microtrauma from thought be major causative factors. Due hypovascular hypocellular nature microenvironment, healing aged related difficult using current pain/inflammation surgical management techniques. Therefore, there need for novel therapies, specifically cellular therapy cell rejuvenation, due decreased regenerative capacity during aging. To augment therapeutic strategies treating tendon-aging-associated diseases injuries, comprehensive understanding needed. This review summarizes age-related changes, including behaviors, extracellular matrix (ECM) composition, biomechanical properties capacity. Additionally, impact conventional treatments (diet, exercise, surgery) discussed, recent advanced (cell rejuvenation) highlighted address healing. underscores molecular linkages between response, provides overview tendons. Understanding underlying rationale future basic translational studies crucial development therapeutics regeneration.

Язык: Английский

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Predominant ligament-centric soft-tissue involvement differentiates axial psoriatic arthritis from ankylosing spondylitis

Nature Reviews Rheumatology, Год журнала: 2023, Номер 19(12), С. 818 - 827

Опубликована: Ноя. 2, 2023

Язык: Английский

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Musculoskeletal Organs‐on‐Chips: An Emerging Platform for Studying the Nanotechnology–Biology Interface

Advanced Materials, Год журнала: 2024, Номер unknown

Опубликована: Март 16, 2024

Nanotechnology-based approaches are promising for the treatment of musculoskeletal (MSK) disorders, which present significant clinical burdens and challenges, but their translation requires a deep understanding complex interplay between nanotechnology MSK biology. Organ-on-a-chip (OoC) systems have emerged as an innovative versatile microphysiological platform to replicate dynamics tissue microenvironment studying nanotechnology-biology interactions. This review first covers recent advances applications OoCs ability mimic biophysical biochemical stimuli encountered by tissues. Next, integrating into OoCs, cellular responses behaviors may be investigated precisely controlling manipulating nanoscale environment. Analysis disease mechanisms, particularly bone, joint, muscle degeneration, drug screening development personalized medicine greatly facilitated using OoCs. Finally, future challenges directions outlined field, including advanced sensing technologies, integration immune-active components, enhancement biomimetic functionality. By highlighting emerging this aims advance intricate nanotechnology-MSK biology interface its significance in management, therapeutic interventional strategies.

Язык: Английский

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The Connection Between Resistance Training, Climbing Performance, and Injury Prevention

Sports Medicine - Open, Год журнала: 2024, Номер 10(1)

Опубликована: Янв. 19, 2024

Abstract Background Climbing is an intricate sport composed of various disciplines, holds, styles, distances between and levels difficulty. In highly skilled climbers the potential for further strength-specific adaptations to increase performance may be marginal in elite climbers. With eye on upcoming 2024 Paris Olympics, more are trying maximize improve training strategies. The relationships muscular strength climbing performance, as well role injury prevention, remain fully elucidated. This narrative review seeks discuss current literature regarding effect resistance improving maximal strength, muscle hypertrophy, power, local endurance a strategy prevent injuries. Main Body Since requires exerting forces against gravity maintain grip move body along route, it generally accepted that climber`s absolute relative important performance. Performance characteristics forearm flexor muscles (hang-time ledge, force output, rate development, oxidative capacity) discriminate level, non-climbers. Strength hand wrist flexors, shoulders upper limbs has gained much attention scientific literature, been suggested both general specific should part program. Furthermore, ability generate sub-maximal different work-rest ratios proved useful, examining finger capacity while mimic real-world demands. Importantly, fingers most frequent locations climbing. Due high mechanical stress load fingerboard campus board limited lower-graded Coaches address, acknowledge, screen amenorrhea disordered eating Conclusion Structured low-volume high-resistance training, twice per week hanging from small ledges or fingerboard, feasible approach prevention aims level through building tolerance performance-relevant exposure promoting this field.

Язык: Английский

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A bioabsorbable mechanoelectric fiber as electrical stimulation suture

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Окт. 8, 2024

In surgical medicine, suturing is the standard treatment for large incisions, yet traditional sutures are limited in functionality. Electrical stimulation a non-pharmacological therapy that promotes wound healing. this context, we designed passive and biodegradable mechanoelectric suture. The suture consists of multi-layer coaxial structure composed (poly(lactic-co-glycolic acid), polycaprolactone) magnesium to allow safe degradation. addition excellent mechanical properties, mechanoelectrical nature grants generation electric fields response movement stretching. This shown speed up healing by 50% reduce risk infection. work presents an evolution conventional closure procedures, using degradable device ready be translated into clinical practice.

Язык: Английский

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Unlocking the Power of the Mediterranean Diet: Two in One—Dual Benefits for Rheumatic and Thyroid Autoimmune Diseases

Nutrients, Год журнала: 2025, Номер 17(8), С. 1383 - 1383

Опубликована: Апрель 19, 2025

In recent years, autoimmune diseases are becoming more and prevalent worldwide, with this rapid rise being influenced by environmental factors linked to lifestyle changes in modern societies. context, the role of diet has been topic extensive research as evidence mounted that particular dietary patterns may contribute or modulate autoimmunity. The present review specifically focuses on Mediterranean (MD) a whole pattern, its peculiar components, such n-3 polyunsaturated fatty acids (PUFAs), polyphenols fiber. We explored their potential benefits spectrum both systemic organ-specific disorders, including rheumatic (like arthritis lupus erythematosus), thyroid Hashimoto’s thyroiditis), since they often occur same individuals. Here, we offer comprehensive about influence these translation into therapeutic interventions, an adjuvant approach improve autoimmunity-related outcomes.

Язык: Английский

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