Parishin A-loaded mesoporous silica nanoparticles modulate macrophage polarization to attenuate tendinopathy DOI Creative Commons
Lisha Zhu, Yu Wang, Shanshan Jin

et al.

npj Regenerative Medicine, Journal Year: 2023, Volume and Issue: 8(1)

Published: March 10, 2023

Macrophages are involved mainly in the balance between inflammation and tenogenesis during healing process of tendinopathy. However, etiological therapeutic strategies to efficiently treat tendinopathy by modulating macrophage state still lacking. In this study, we find that a small molecule compound Parishin-A (PA) isolated from Gastrodia elata could promote anti-inflammatory M2 polarization inhibiting gene transcription protein phosphorylation signal transducers activators 1. Local injection or sustained delivery PA mesoporous silica nanoparticles (MSNs) almost recover native tendon's dense parallel-aligned collagen matrix collagenase-induced macrophage-mediated immune microenvironment preventing heterotopic ossification. Especially, MSNs decrease doses PA, frequency yield preferable effects. Mechanistically, intervention with indirectly inhibit activation mammalian target rapamycin repress chondrogenic osteogenic differentiation tendon stem/progenitor cells influencing inflammatory cytokine secretion. Together, pharmacological natural small-molecule modulate status appears be promising strategy for treatment.

Language: Английский

Co‐Electrospun Silk Fibroin and Gelatin Methacryloyl Sheet Seeded with Mesenchymal Stem Cells for Tendon Regeneration DOI
Yumeng Xue, Han‐Jun Kim, Junmin Lee

et al.

Small, Journal Year: 2022, Volume and Issue: 18(21)

Published: April 29, 2022

Abstract Silk fibroin (SF) is a promising biomaterial for tendon repair, but its relatively rigid mechanical properties and low cell affinity have limited application in regenerative medicine. Meanwhile, gelatin‐based polymers advantages attachment tissue remodeling insufficient strength to regenerate tough such as tendons. Taking these aspects into account, this study, gelatin methacryloyl (GelMA) combined with SF create mechanically strong bioactive nanofibrous scaffold (SG). The of SG nanofibers can be flexibly modulated by varying the ratio GelMA. Compared nanofibers, mesenchymal stem cells (MSCs) seeded on fibers optimal composition (SG7) exhibit enhanced growth, proliferation, vascular endothelial growth factor production, tenogenic gene expression behavior. Conditioned media from MSCs cultured SG7 scaffolds greatly promote migration proliferation tenocytes. Histological analysis tenogenesis‐related immunofluorescence staining indicate demonstrate vivo regeneration compared other groups. Therefore, rational combinations GelMA hybrid may help improve therapeutic outcomes address challenges tissue‐engineered regeneration.

Language: Английский

Citations

49
Exercise for Osteoarthritis: A Literature Review of Pathology and Mechanism DOI Creative Commons
Hui Kong, Xue‐Qiang Wang, Xinan Zhang

et al.

Frontiers in Aging Neuroscience, Journal Year: 2022, Volume and Issue: 14

Published: May 3, 2022

Osteoarthritis (OA) has a very high incidence worldwide and become common joint disease in the elderly. Currently, treatment methods for OA include surgery, drug therapy, exercise therapy. In recent years, of certain diseases by received increasing research attention. Proper can improve physiological function various organs body. At present, is usually symptomatic. Limited are available according to its pathogenesis, effective intervention not been developed slow down progress from molecular level. Only clarifying mechanism influence different intensities on patients we choose appropriate prescription prevent treat OA. This review mainly expounds that alleviates pathological changes affecting degradation ECM, apoptosis, inflammatory response, autophagy, ncRNA, summarizes effects types patients. Finally, it found types, intensity, time frequency have same time, suitable prescriptions recommended

Language: Английский

Citations

43
TMT-Based Quantitative Proteomics Analysis of Synovial Fluid-Derived Exosomes in Inflammatory Arthritis DOI Creative Commons

Yukai Huang,

Yuqi Liu,

Qidang Huang

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: March 11, 2022

To compare the proteomics of synovial fluid (SF)-derived exosomes in rheumatoid arthritis (RA), axial spondyloarthritis (axSpA), gout, and osteoarthritis (OA) patients.Exosomes were separated from SF by Exoquick kit combined ultracentrifugation method. Tandem mass tags (TMT)-labeled liquid chromatography spectrometry (LC-MS/MS) technology was used to analyze SF-derived exosomes. Volcano plot, hierarchical cluster, gene ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis conducted.A total 1,678 credible proteins detected. Sixty-nine differentially expressed found compared with OA, axSpA, RA simultaneously. Twenty-five highly gout uniquely, lysozyme C protein S100-A9 included, whose bioinformatic significantly involved "neutrophil degranulation" "prion diseases". Eighty-four Thirty-nine axSpA RNA-binding 8A transport Sec24C "acute-phase response" "citrate cycle". One hundred eighty-four RA, Twenty-eight pregnancy zone (PZP) stromelysin-1 "serine-type endopeptidase inhibitor activity" "complement coagulation cascades". Enzyme-linked immunosorbent assay (ELISA) result showed that exosome-derived PZP level higher than OA (p < 0.05).Our study for first time described profiles patients. Some potential biomarkers hypothetical molecular mechanisms proposed, which may provide helpful diagnostic therapeutic insights inflammatory (IA).

Language: Английский

Citations

42
Effect of Aging on Tendon Biology, Biomechanics and Implications for Treatment Approaches DOI Open Access
Ka Yu Carissa Kwan, K.W. Ng,

Ying Rao

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(20), P. 15183 - 15183

Published: Oct. 14, 2023

Tendon aging is associated with an increasing prevalence of tendon injuries and/or chronic diseases, such as tendinopathy, which affects approximately 25% the adult population. Aged tendons are often characterized by a reduction in number and functionality stem/progenitor cells (TSPCs), fragmented or disorganized collagen bundles, increased deposition glycosaminoglycans (GAGs), leading to pain, inflammation, impaired mobility. Although exact pathology unknown, overuse microtrauma from thought be major causative factors. Due hypovascular hypocellular nature microenvironment, healing aged related difficult using current pain/inflammation surgical management techniques. Therefore, there need for novel therapies, specifically cellular therapy cell rejuvenation, due decreased regenerative capacity during aging. To augment therapeutic strategies treating tendon-aging-associated diseases injuries, comprehensive understanding needed. This review summarizes age-related changes, including behaviors, extracellular matrix (ECM) composition, biomechanical properties capacity. Additionally, impact conventional treatments (diet, exercise, surgery) discussed, recent advanced (cell rejuvenation) highlighted address healing. underscores molecular linkages between response, provides overview tendons. Understanding underlying rationale future basic translational studies crucial development therapeutics regeneration.

Language: Английский

Citations

30
Predominant ligament-centric soft-tissue involvement differentiates axial psoriatic arthritis from ankylosing spondylitis DOI
Dennis McGonagle, Paula David, Tom Macleod

et al.

Nature Reviews Rheumatology, Journal Year: 2023, Volume and Issue: 19(12), P. 818 - 827

Published: Nov. 2, 2023

Language: Английский

Citations

23
Musculoskeletal Organs‐on‐Chips: An Emerging Platform for Studying the Nanotechnology–Biology Interface DOI Creative Commons
Yuwen Wang, Patrick Shu‐Hang Yung, Gang Lü

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: March 16, 2024

Nanotechnology-based approaches are promising for the treatment of musculoskeletal (MSK) disorders, which present significant clinical burdens and challenges, but their translation requires a deep understanding complex interplay between nanotechnology MSK biology. Organ-on-a-chip (OoC) systems have emerged as an innovative versatile microphysiological platform to replicate dynamics tissue microenvironment studying nanotechnology-biology interactions. This review first covers recent advances applications OoCs ability mimic biophysical biochemical stimuli encountered by tissues. Next, integrating into OoCs, cellular responses behaviors may be investigated precisely controlling manipulating nanoscale environment. Analysis disease mechanisms, particularly bone, joint, muscle degeneration, drug screening development personalized medicine greatly facilitated using OoCs. Finally, future challenges directions outlined field, including advanced sensing technologies, integration immune-active components, enhancement biomimetic functionality. By highlighting emerging this aims advance intricate nanotechnology-MSK biology interface its significance in management, therapeutic interventional strategies.

Language: Английский

Citations

12
The Connection Between Resistance Training, Climbing Performance, and Injury Prevention DOI Creative Commons
Atle Hole Sæterbakken, Nicolay Stien,

Helene Pedersen

et al.

Sports Medicine - Open, Journal Year: 2024, Volume and Issue: 10(1)

Published: Jan. 19, 2024

Abstract Background Climbing is an intricate sport composed of various disciplines, holds, styles, distances between and levels difficulty. In highly skilled climbers the potential for further strength-specific adaptations to increase performance may be marginal in elite climbers. With eye on upcoming 2024 Paris Olympics, more are trying maximize improve training strategies. The relationships muscular strength climbing performance, as well role injury prevention, remain fully elucidated. This narrative review seeks discuss current literature regarding effect resistance improving maximal strength, muscle hypertrophy, power, local endurance a strategy prevent injuries. Main Body Since requires exerting forces against gravity maintain grip move body along route, it generally accepted that climber`s absolute relative important performance. Performance characteristics forearm flexor muscles (hang-time ledge, force output, rate development, oxidative capacity) discriminate level, non-climbers. Strength hand wrist flexors, shoulders upper limbs has gained much attention scientific literature, been suggested both general specific should part program. Furthermore, ability generate sub-maximal different work-rest ratios proved useful, examining finger capacity while mimic real-world demands. Importantly, fingers most frequent locations climbing. Due high mechanical stress load fingerboard campus board limited lower-graded Coaches address, acknowledge, screen amenorrhea disordered eating Conclusion Structured low-volume high-resistance training, twice per week hanging from small ledges or fingerboard, feasible approach prevention aims level through building tolerance performance-relevant exposure promoting this field.

Language: Английский

Citations

11
A bioabsorbable mechanoelectric fiber as electrical stimulation suture DOI Creative Commons

Zhouquan Sun,

Yuefan Jin,

Jiabei Luo

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 8, 2024

In surgical medicine, suturing is the standard treatment for large incisions, yet traditional sutures are limited in functionality. Electrical stimulation a non-pharmacological therapy that promotes wound healing. this context, we designed passive and biodegradable mechanoelectric suture. The suture consists of multi-layer coaxial structure composed (poly(lactic-co-glycolic acid), polycaprolactone) magnesium to allow safe degradation. addition excellent mechanical properties, mechanoelectrical nature grants generation electric fields response movement stretching. This shown speed up healing by 50% reduce risk infection. work presents an evolution conventional closure procedures, using degradable device ready be translated into clinical practice.

Language: Английский

Citations

10
Single cell and spatial transcriptomics in human tendon disease indicate dysregulated immune homeostasis DOI Creative Commons
Moeed Akbar, Lucy MacDonald,

Lindsay A. N. Crowe

et al.

Annals of the Rheumatic Diseases, Journal Year: 2021, Volume and Issue: 80(11), P. 1494 - 1497

Published: May 17, 2021

Figure 1 The frequency and OR of ultrasound power Doppler signal in tender vs non-tender non-swollen wrists.

Language: Английский

Citations

55
Nanomaterials for the Diagnosis and Treatment of Inflammatory Arthritis DOI Open Access
Seyedeh Maryam Hosseinikhah, Mahmood Barani, Abbas Rahdar

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(6), P. 3092 - 3092

Published: March 18, 2021

Nanomaterials have received increasing attention due to their unique chemical and physical properties for the treatment of rheumatoid arthritis (RA), most common complex multifactorial joint-associated autoimmune inflammatory disorder. RA is characterized by an inflammation synovium with increased production proinflammatory cytokines (IL-1, IL-6, IL-8, IL-10) destruction articular cartilage bone, it associated development cardiovascular disorders such as heart attack stroke. While a number imaging tools allow monitoring diagnosis arthritis, despite ongoing work enhance sensitivity precision, proper assessment remains difficult particularly in early stages disease. Our goal here describe benefits applying various nanomaterials next-generation detection using contrast agents nanosensors improved drug delivery systems effective

Language: Английский

Citations

43