A single-cell resolution gene expression atlas of the larval zebrafish brain

Science Advances, Год журнала: 2023, Номер 9(8)

Опубликована: Фев. 22, 2023

The advent of multimodal brain atlases promises to accelerate progress in neuroscience by allowing silico queries neuron morphology, connectivity, and gene expression. We used multiplexed fluorescent situ RNA hybridization chain reaction (HCR) technology generate expression maps across the larval zebrafish for a growing set marker genes. data were registered Max Planck Zebrafish Brain (mapzebrain) atlas, thus covisualization expression, single-neuron tracings, expertly curated anatomical segmentations. Using post hoc HCR labeling immediate early cfos, we mapped responses prey stimuli food ingestion freely swimming larvae. This unbiased approach revealed, addition previously described visual motor areas, cluster neurons secondary gustatory nucleus, which express calb2a, as well specific neuropeptide Y receptor, project hypothalamus. discovery exemplifies power this new atlas resource neurobiology.

Язык: Английский

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High-throughput functional analysis of autism genes in zebrafish identifies convergence in dopaminergic and neuroimmune pathways

Cell Reports, Год журнала: 2023, Номер 42(3), С. 112243 - 112243

Опубликована: Март 1, 2023

Advancing from gene discovery in autism spectrum disorders (ASDs) to the identification of biologically relevant mechanisms remains a central challenge. Here, we perform parallel vivo functional analysis 10 ASD genes at behavioral, structural, and circuit levels zebrafish mutants, revealing both unique overlapping effects loss function. Whole-brain mapping identifies forebrain cerebellum as most significant contributors brain size differences, while regions involved sensory-motor control, particularly dopaminergic regions, are associated with altered baseline activity. Finally, show global increase microglia resulting function select implicating neuroimmune dysfunction key pathway biology.

Язык: Английский

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Transcriptomic neuron types vary topographically in function and morphology

Nature, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

Abstract Neuronal phenotypic traits such as morphology, connectivity and function are dictated, to a large extent, by specific combination of differentially expressed genes. Clusters neurons in transcriptomic space correspond distinct cell types some cases—for example, Caenorhabditis elegans 1 retinal ganglion cells 2–4 —have been shown share morphology function. The zebrafish optic tectum is composed spatial array that transforms visual inputs into motor outputs. Although the visuotopic map continuous, subregions functionally specialized 5,6 . Here, uncover cell-type architecture tectum, we transcriptionally profiled its neurons, revealing more than 60 organized anatomical layers. We measured responses thousands tectal two-photon calcium imaging matched them with their transcriptional profiles. Furthermore, characterized morphologies identified using transgenic lines. Notably, found similar can diverge shape, responses. Incorporating coordinates within volume revealed morphologically defined subclusters individual clusters. Our findings demonstrate extrinsic, position-dependent factors expand repertoire genetically neurons.

Язык: Английский

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Whole-brain imaging of freely-moving zebrafish

Frontiers in Neuroscience, Год журнала: 2023, Номер 17

Опубликована: Апрель 17, 2023

One of the holy grails neuroscience is to record activity every neuron in brain while an animal moves freely and performs complex behavioral tasks. While important steps forward have been taken recently large-scale neural recording rodent models, single resolution across entire mammalian remains elusive. In contrast larval zebrafish offers great promise this regard. Zebrafish are a vertebrate model with substantial homology brain, but their transparency allows whole-brain recordings genetically-encoded fluorescent indicators at single-neuron using optical microscopy techniques. Furthermore begin show repertoire natural behavior from early age, including hunting small, fast-moving prey visual cues. Until work address bases these behaviors mostly relied on assays where fish was immobilized under microscope objective, stimuli such as were presented virtually. However significant progress has made developing imaging techniques for which not immobilized. Here we discuss recent advances, focusing particularly based light-field microscopy. We also draw attention several outstanding issues remain be addressed increase ecological validity results obtained.

Язык: Английский

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Development of neural circuits for social motion perception in schooling fish

Current Biology, Год журнала: 2024, Номер 34(15), С. 3380 - 3391.e5

Опубликована: Июль 17, 2024

The collective behavior of animal groups emerges from the interactions among individuals. These social produce coordinated movements bird flocks and fish schools, but little is known about their developmental emergence neurobiological foundations. By characterizing visually based schooling micro glassfish Danionella cerebrum, we found that development progresses sequentially, with animals first acquiring ability to aggregate, followed by postural alignment partners. This maturation was accompanied neural populations in midbrain were preferentially driven visual stimuli resemble shape fish. Furthermore, isolation over course impaired both encoding motion adults. work demonstrates selective for form conspecifics emerge experience-dependent movement.

Язык: Английский

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Diencephalic and Neuropeptidergic Dysfunction in Zebrafish with Autism Risk Mutations

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 20, 2024

Abstract Hundreds of human mutations are linked to autism and related disorders, yet the functions many these mutated genes during vertebrate neurodevelopment unclear. We generated 27 zebrafish mutants with presumptive protein-truncating or specific missense variants corresponding autism-risk alleles in 17 genes. observed baseline stimulus-driven behavioral changes at larval stages, as well social behavior differences lines tested juveniles. Imaging whole-brain activity revealed a near identical map for unrelated kmt5b hdlbpa , defined by increased mainly diencephalon. Mutating 7 risk resulted substantial brain size differences. Using RNA sequencing, we further molecular drivers phenotypes, identifying targetable disruptions neuropeptide signaling, neuronal maturation, cell proliferation. This multi-modal screen nominated regions, types, pathways that may contribute susceptibility. Teaser Zebrafish uncovers diencephalon, interaction, neuropeptidergic signaling phenotypes ASD mutants.

Язык: Английский

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The Visual Systems of Zebrafish

Annual Review of Neuroscience, Год журнала: 2024, Номер 47(1), С. 255 - 276

Опубликована: Апрель 25, 2024

The zebrafish visual system has become a paradigmatic preparation for behavioral and systems neuroscience. Around 40 types of retinal ganglion cells (RGCs) serve as matched filters stimulus features, including light, optic flow, prey, objects on collision course. RGCs distribute their signals via axon collaterals to 12 retinorecipient areas in forebrain midbrain. major visuomotor hub, the tectum, harbors nine RGC input layers that combine information multiple features. retinotopic map tectum is locally adapted scene statistics subfield–specific demands. Tectal projections premotor centers are topographically organized according commands. known connectivity more than 20 processing streams allows us dissect cellular basis elementary perceptual cognitive functions. Visually evoked responses, such prey capture or loom avoidance, controlled by dedicated multistation pathways that—at least larva—resemble labeled lines. This architecture serves neuronal code's purpose driving adaptive behavior.

Язык: Английский

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Expression of glucocorticoid-receptor covaries with individual differences in visual lateralisation in zebrafish

Animal Cognition, Год журнала: 2025, Номер 28(1)

Опубликована: Март 13, 2025

Abstract Cerebral lateralisation, the differential cognitive processing in two brain hemispheres, is variable among individuals most vertebrates. Part of this variance has been attributed to plasticity response environmental stressors experienced by and might be therefore mediated action glucocorticoids (GCs). Accordingly, we tested hypothesis that GC pathway related stress, which involved its cognate receptor GR, affects individuals’ lateralisation. First, characterised behavioural lateralisation phenotype outbred wild-type zebrafish using three different tests: a motor test, test involving visual social stimulus (subject’s mirror image), with negative valence (predator). Subsequently, quantified expression gr gene subjects, specifically telencephalon mesencephalon each hemisphere. Our population exhibited individual variation but no population-level bias across hemispheres. When correlated patterns tests expression, observed higher mesencephalic right hemisphere were more inclined process their image Additionally, telencephalon, showed reduced predator stimulus. This study supports GCs affect some aspects particular those stimuli, thought GC-Gr suggests intraspecific could result from differences expression.

Язык: Английский

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A thalamo-preoptic pathway promotes social grooming in rodents

Current Biology, Год журнала: 2022, Номер 32(21), С. 4593 - 4606.e8

Опубликована: Сен. 15, 2022

Язык: Английский

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Structure and function in artificial, zebrafish and human neural networks

Physics of Life Reviews, Год журнала: 2023, Номер 45, С. 74 - 111

Опубликована: Апрель 25, 2023

Язык: Английский

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