Xenotopic synthetic biology: Prospective tools for delaying aging and age-related diseases DOI Creative Commons
Andrey A. Parkhitko, Valentin Cracan

Science Advances, Год журнала: 2025, Номер 11(13)

Опубликована: Март 28, 2025

Metabolic dysregulation represents one of the major driving forces in aging. Although multiple genetic and pharmacological manipulations are known to extend longevity model organisms, aging is a complex trait, targeting one’s own genes may be insufficient prevent age-dependent deterioration. An alternative strategy could use enzymes from other species reverse age-associated metabolic changes. In this review, we discuss set lower organisms that have been shown affect various parameters linked age-related processes. These include modulators steady-state levels amino acids (METase, ASNase, ADI), NADPH/NADP + and/or reduced form coenzyme Q (CoQH 2 )/CoQ redox potentials (NDI1, AOX, Lb NOX, TPNOX, Ec STH, RquA, LOXCAT, Grubraw, ScURA), GSH (StGshF), mitochondrial membrane potential (mtON mito-dR), or reactive oxygen (DAAO KillerRed-SOD1). We propose leveraging non-mammalian an untapped resource can used delay diseases.

Язык: Английский

High-performance silk fibroin/hyaluronic acid interpenetrating network hydrogel microneedles for diabetes management DOI

Jiahui Hua,

Renyan Huang,

Yu Meng

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 298, С. 140357 - 140357

Опубликована: Янв. 29, 2025

Язык: Английский

Процитировано

1
Aging research comes of age DOI Open Access

Vivien Marx

Nature Methods, Год журнала: 2024, Номер 21(1), С. 11 - 15

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

5
Enzyme-mediated green synthesis of glycosaminoglycans and catalytic process intensification DOI
Jie Zheng,

Xiao-Jun Lin,

Xu Han

и другие.

Biotechnology Advances, Год журнала: 2024, Номер 74, С. 108394 - 108394

Опубликована: Июнь 8, 2024

Язык: Английский

Процитировано

5
Hyaluronan‐protein interactions: Lilliput revisited DOI Creative Commons
Anthony J. Day

Proteoglycan Research, Год журнала: 2024, Номер 2(4)

Опубликована: Окт. 1, 2024

Abstract Hyaluronan (HA) is a huge linear polysaccharide composed entirely of simple repeating disaccharide that has been preserved unchanged since the evolution vertebrates ~520 million years ago. It present in all mammalian tissues, being synthesised within cell membrane and extruded into extracellular space where it dictates tissue elasticity, hydration permeability. HA also directs behaviour via engagement with surface receptors. These properties allow to mediate diverse functions wide range physiological pathological processes, including development, reproduction inflammation. There growing evidence way which biopolymer differentially organised, through its interaction repertoire HA‐binding proteins (HABPs), key diversity biological functions. This review summarises current knowledge HA‐protein interactions how their may lead formation HA/protein complexes distinct molecular architectures turn underpin different physical receptor‐mediated effects. Potentially controversial areas such pro‐inflammatory effects low weight fragments short peptides modulate function are considered from perspective.

Язык: Английский

Процитировано

5
Enzyme-crosslinked hyaluronic acid hydrogel scaffolds for BMSCs microenvironment and wound healing DOI
Bingjie Cai, Jiansong Fang, Shengxi Zhou

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 295, С. 139566 - 139566

Опубликована: Янв. 8, 2025

Язык: Английский

Процитировано

0
Bioactive Glycyrrhizic Acid Ionic Liquid Self‐Assembled Nanomicelles for Enhanced Transdermal Delivery of Anti‐Photoaging Signal Peptides DOI Creative Commons
Zhuxian Wang, Jun Liu, Qiuyu Chen

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Abstract Sigal peptides have garnered remarkable efficacy in rejuvenating photoaged skin and delaying senescence. Nevertheless, their low solubility poor permeability bring about a formidable challenge transdermal delivery. To address this challenge, bioactive ionic liquids (ILs) synthesized from natural glycyrrhizic acid (GA) oxymatrine (OMT) with eminent biocompatibility is first prepared. The components ratios inherent forming mechanisms of GA‐OMT (GAO) are optimized by molecular dynamics simulations density functional theory calculations. Remarkably, GAO can significantly improve the sparingly soluble properties palmitoyl pentapeptide‐4 (PAL‐4), model peptide drug. Subsequently, self‐assembled micelles loading PAL‐4 (GAO/PAL‐4‐SM) fabricated without additional auxiliary materials. permeation subcutaneous retention promoted 10wt.% GAO‐SM. Moreover, ILs facilitated enhancing its miscibility interaction stratum corneum (SC), offering pulling effect micellar structures for PAL‐4, as elucidated computational simulations. In cellular animal photoaging experiments, GAO/PAL‐4‐SM possessed capabilities boosting collagen hyaluronic regeneration, mitigating inflammation apoptosis, accelerating macrophage M2 polarization, thereby lessening wrinkles leveraging elasticity. Collectively, research innovatively designed an nano‐micellar delivery system to enhance anti‐photoaging signal peptides.

Язык: Английский

Процитировано

0
Counteracting immunodepression by extracellular matrix hydrogel to promote brain tissue remodeling and neurological function recovery after traumatic brain injury DOI Creative Commons
Bixue Wang,

qiaoping zhang,

Changsheng Liu

и другие.

Biomaterials, Год журнала: 2025, Номер 318, С. 123181 - 123181

Опубликована: Фев. 8, 2025

Язык: Английский

Процитировано

0
The translational potential of studying bat immunity DOI
Kaushal Baid, Aaron T. Irving, Nolwenn Jouvenet

и другие.

Trends in Immunology, Год журнала: 2024, Номер 45(3), С. 188 - 197

Опубликована: Март 1, 2024

Язык: Английский

Процитировано

4
IL6/adiponectin/HMGB1 feedback loop mediates adipocyte and macrophage crosstalk and M2 polarization after myocardial infarction DOI Creative Commons
Yue Zheng, Yu‐Chao Wang, Bingcai Qi

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Март 27, 2024

Background Differences in border zone contribute to different outcomes post-infarction, such as left ventricular aneurysm (LVA) and myocardial infarction (MI). LVA usually forms within 24 h of the onset MI may cause heart rupture; however, surgery is best performed 3 months after MI. Few studies have investigated model, differences zones between MI, mechanism zone. Methods The LVA, SHAM mouse models were used. Echocardiography, Masson’s trichrome staining, immunofluorescence staining performed, RNA sequencing was conducted. adipocyte-conditioned medium-treated hypoxic macrophage cell line employed determine effects hub gene, adiponectin ( ADPN ), on macrophages. Quantitative polymerase chain reaction (qPCR), Western blot analysis, transmission electron microscopy, chromatin immunoprecipitation (ChIP) assays conducted elucidate Human subepicardial adipose tissue blood samples collected validate ADPN. Results A novel, simple, consistent, low-cost model constructed. caused a greater reduction contractile functions than owing reduced wall thickness edema impeded cardiac promoted lymphangiogenesis by increasing infiltration post-infarction. Adipocyte-derived M2 polarization sustained mitochondrial quality via ADPN/AdipoR2/HMGB1 axis. Mechanistically, HMGB1 inflammation decreased interleukin-6 (IL6) secretion. secretion IL6 increased expression STAT3 co-transcription factor, YAP, adipocytes. Based ChIP Dual-Glo luciferase experiments, transcription binding its promoter In vivo , injury These phenotypes rescued depletion or knockdown Supplying adipocytes overexpressing collagen disposition, lymphangiogenesis, impaired injury. However, these Overall, IL6/ADPN/HMGB1 axis validated using human samples. This could serve an independent factor overweight patients who need coronary artery bypass grafting (CABG) treatment. Conclusion loop macrophages contributes clinical Thus, targeting be novel therapeutic approach for lymphatic regulation senescence

Язык: Английский

Процитировано

4
Local injection of a freshly manufactured 35 kDa hyaluronan fragment reduces neuropathic and inflammatory pain: A clinical study DOI Creative Commons

Fenghe Xu,

Dylan Treger,

Xinyue Ma

и другие.

European Journal of Inflammation, Год журнала: 2024, Номер 22

Опубликована: Янв. 1, 2024

To investigate the ability of 35 kDa low-molecular-weight hyaluronan fragment (HA35) to relieve neuropathic and inflammatory pain, including postherpetic neuralgia shoulder, neck, back temporomandibular pain. Ten patients with 26 or pain were studied assessed. The was prepared by mixing hyaluronidase 100 mg high-molecular-weight HA at room temperature for 20 mins. This mixture locally injected once point where nerve trunk innervated point. Patients scored their comfort on numerical rating scale (NPRS) General Comfort Questionnaire (GCQ). After treatment, NPRS scores GCQ improved. had significantly lower 30 min 180 after injection, especially ( p < .001). treatment 24 h greater than that before .01). No adverse reactions occurred. effectively relieved neuralgia-induced Clinical Trial Registration: NCT05809700 https://www.clinicaltrials.gov .

Язык: Английский

Процитировано

4