CAVE: Connectome Annotation Versioning Engine DOI Creative Commons
Sven Dorkenwald, Casey M Schneider-Mizell, Derrick Brittain

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июль 28, 2023

Abstract Advances in Electron Microscopy, image segmentation and computational infrastructure have given rise to large-scale richly annotated connectomic datasets which are increasingly shared across communities. To enable collaboration, users need be able concurrently create new annotations correct errors the automated by proofreading. In large datasets, every proofreading edit relabels cell identities of millions voxels thousands like synapses. For analysis, require immediate reproducible access this constantly changing expanding data landscape. Here, we present Connectome Annotation Versioning Engine (CAVE), a for connectome analysis up-to petascale (∼1mm 3 ) while annotating is ongoing. segmentation, CAVE provides distributed continuous versioning reconstructions. Annotations defined locations such that they can quickly assigned underlying segment enables fast queries CAVE’s arbitrary time points. supports schematized, extensible annotations, so researchers readily design novel annotation types. already used many connectomics including largest available date.

Язык: Английский

Organization of an ascending circuit that conveys flight motor state in Drosophila DOI Creative Commons
Han SJ Cheong,

Kaitlyn Nicole Boone,

Marryn M. Bennett

и другие.

Current Biology, Год журнала: 2024, Номер 34(5), С. 1059 - 1075.e5

Опубликована: Фев. 22, 2024

Язык: Английский

Процитировано

15
Neural circuit mechanisms underlying context-specific halting in Drosophila DOI Creative Commons

Neha Sapkal,

Nino Mancini,

Divya Sthanu Kumar

и другие.

Nature, Год журнала: 2024, Номер 634(8032), С. 191 - 200

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

15
Social state gates vision using three circuit mechanisms inDrosophila DOI Creative Commons
Catherine E. Schretter, Tom Hindmarsh Sten, Nathan C Klapoetke

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 17, 2024

Abstract Animals are often bombarded with visual information and must prioritize specific features based on their current needs. The neuronal circuits that detect relay have been well-studied. Yet, much less is known about how an animal adjusts its attention as goals or environmental conditions change. During social behaviors, flies need to focus nearby flies. Here, we study the flow of altered when female Drosophila enter aggressive state. From connectome, identified three state-dependent circuit motifs poised selectively amplify response fly-sized objects: convergence excitatory inputs from neurons conveying select internal state; dendritic disinhibition feature detectors; a switch toggles between two detectors. Using cell-type-specific genetic tools, together behavioral neurophysiological analyses, show each these function during aggression. We reveal this same operate in males courtship pursuit, suggesting disparate behaviors may share mechanisms. Our work provides compelling example using connectome infer mechanisms underlie dynamic processing sensory signals.

Язык: Английский

Процитировано

14
Mapping model units to visual neurons reveals population code for social behaviour DOI Creative Commons
Benjamin R. Cowley, Adam J. Calhoun,

Nivedita Rangarajan

и другие.

Nature, Год журнала: 2024, Номер 629(8014), С. 1100 - 1108

Опубликована: Май 22, 2024

Abstract The rich variety of behaviours observed in animals arises through the interplay between sensory processing and motor control. To understand these sensorimotor transformations, it is useful to build models that predict not only neural responses input 1–5 but also how each neuron causally contributes behaviour 6,7 . Here we demonstrate a novel modelling approach identify one-to-one mapping internal units deep network real neurons by predicting behavioural changes arise from systematic perturbations more than dozen neuronal cell types. A key ingredient introduce ‘knockout training’, which involves perturbing during training match experiments. We apply this model transformations Drosophila melanogaster males complex, visually guided social 8–11 visual projection at interface optic lobe central brain form set discrete channels 12 , prior work indicates channel encodes specific feature drive particular 13,14 Our reaches different conclusion: combinations neurons, including those involved non-social behaviours, male interactions with female, forming population code for behaviour. Overall, our framework consolidates effects elicited various into single, unified model, providing map stimulus type behaviour, enabling future incorporation wiring diagrams 15 model.

Язык: Английский

Процитировано

12
Comparative connectomics of the descending and ascending neurons of theDrosophilanervous system: stereotypy and sexual dimorphism DOI Creative Commons
Tomke Stürner, P. D. Brooks, Laia Serratosa Capdevila

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 6, 2024

In most complex nervous systems there is a clear anatomical separation between the nerve cord, which contains of final motor outputs necessary for behaviour, and brain. insects, neck connective both physical information bottleneck connecting brain ventral cord (VNC, spinal analogue) comprises diverse populations descending (DN), ascending (AN) sensory neurons, are crucial sensorimotor signalling control. Integrating three separate EM datasets, we now provide complete connectomic description neurons female system

Язык: Английский

Процитировано

11
The fly connectome reveals a path to the effectome DOI Creative Commons
Dean A. Pospisil, Max Jameson Aragon, Sven Dorkenwald

и другие.

Nature, Год журнала: 2024, Номер 634(8032), С. 201 - 209

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

10
Synaptic promiscuity in brain development DOI Creative Commons
Neele Wolterhoff, P. Robin Hiesinger

Current Biology, Год журнала: 2024, Номер 34(3), С. R102 - R116

Опубликована: Фев. 1, 2024

Precise synaptic connectivity is a prerequisite for the function of neural circuits, yet individual neurons, taken out their developmental context, readily form unspecific synapses. How does genome encode brain wiring in light this apparent contradiction? Synaptic specificity outcome long series processes and mechanisms before, during after synapse formation. much promiscuity permissible or necessary at moment partner choice depends on extent to which prior development restricts available partners subsequent corrects initially made can thereby play important roles precise connectivity, but also facilitate flexibility robustness. In review, we assess experimental evidence prevalence promiscuous formation development. Many well-established approaches are based genetic perturbation an assessment only adult; make it difficult pinpoint when given defect mechanism occurred. many cases, such studies reveal that restrict availability already Subsequently, choice, factors including competency, interaction dynamics molecular recognition further partners. The discussion through lens suggests algorithmic process neurons capable continuously prevented from making wrong choices, with no single time point sufficient explain outcome.

Язык: Английский

Процитировано

8
Molecular and cellular mechanisms of teneurin signaling in synaptic partner matching DOI Creative Commons

Chuanyun Xu,

Zhuoran Li, Cheng Lyu

и другие.

Cell, Год журнала: 2024, Номер 187(18), С. 5081 - 5101.e19

Опубликована: Июль 11, 2024

In developing brains, axons exhibit remarkable precision in selecting synaptic partners among many non-partner cells. Evolutionarily conserved teneurins are transmembrane proteins that instruct partner matching. However, how intracellular signaling pathways execute teneurins' functions is unclear. Here, we use situ proximity labeling to obtain the interactome of a teneurin (Ten-m) Drosophila brain. Genetic interaction studies using quantitative matching assays both olfactory receptor neurons (ORNs) and projection (PNs) reveal common pathway: Ten-m binds negatively regulates RhoGAP, thus activating Rac1 small GTPases promote Developmental analyses with single-axon resolution identify cellular mechanism matching: promotes local F-actin levels stabilizes ORN axon branches contact PN dendrites. Combining spatial proteomics high-resolution phenotypic analyses, this study advanced our understanding molecular mechanisms

Язык: Английский

Процитировано

8
Predicting visual function by interpreting a neuronal wiring diagram DOI Creative Commons
H. Sebastian Seung

Nature, Год журнала: 2024, Номер 634(8032), С. 113 - 123

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

8
Synaptic connectome of the Drosophila circadian clock DOI Creative Commons
Nils Reinhard,

Ayumi Fukuda,

Giulia Manoli

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 5, 2024

Abstract The circadian clock and its output pathways play a pivotal role in optimizing daily processes. To obtain insights into how diverse rhythmic physiology behaviors are orchestrated, we have generated comprehensive connectivity map of an animal using the Drosophila FlyWire brain connectome. Intriguingly, identified additional dorsal neurons, thus showing that network contains ~240 instead 150 neurons. We revealed extensive contralateral synaptic within discovered novel indirect light input to also elucidated via which modulates descending neurons known regulate feeding reproductive behaviors. Interestingly, observed sparse monosynaptic between downstream higher-order centers neurosecretory cells behavior physiology. Therefore, integrated single-cell transcriptomics receptor mapping decipher putative paracrine peptidergic signaling by Our analyses neuropeptides expressed suggest significantly enriches interconnectivity network.

Язык: Английский

Процитировано

8