Abstract
Visualizing
the
structure
and
dynamics
of
biomolecules
is
critical
to
understand
biological
function,
requires
methods
fluorescently
label
targets
interest
in
their
cellular
context.
Self‐labelling
proteins,
which
combine
a
genetically
encoded
tag
with
small‐molecule
fluorophore,
have
attracted
considerable
attention
for
this
purpose,
as
they
can
overcome
limitations
fluorescent
proteins.
Among
them,
HaloTag
protein
most
broadly
used,
showing
fast
specific
labelling
small,
easy
functionalize
cell‐permeant
ligand.
Synthetic
chemistry
engineering
provided
portfolio
powerful
imaging
tools
exploiting
HaloTag,
along
general
optimize
adapt
them
applications.
Here,
we
provide
an
overview
reporters
based
on
biosensing,
highlighting
strategies
Neuron,
Год журнала:
2022,
Номер
110(17), С. 2815 - 2835.e13
Опубликована: Июль 8, 2022
Dynamin
mediates
fission
of
vesicles
from
the
plasma
membrane
during
endocytosis.
Typically,
dynamin
is
recruited
cytosol
to
endocytic
sites,
requiring
seconds
tens
seconds.
However,
ultrafast
endocytosis
in
neurons
internalizes
as
quickly
50
ms
synaptic
vesicle
recycling.
Here,
we
demonstrate
that
1
pre-recruited
sites
for
Specifically,
1xA,
a
splice
variant
1,
interacts
with
Syndapin
form
molecular
condensates
on
membrane.
Single-particle
tracking
1xA
molecules
confirms
liquid-like
property
vivo.
When
mutated
disrupt
its
interaction
do
not
form,
and
consequently,
slows
down
by
100-fold.
Mechanistically,
acts
an
adaptor
binding
stores
at
sites.
This
cache
bypasses
recruitment
step
accelerates
synapses.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(42), С. 23000 - 23013
Опубликована: Окт. 16, 2023
Rhodamine
dyes
are
excellent
scaffolds
for
developing
a
broad
range
of
fluorescent
probes.
A
key
property
rhodamines
is
their
equilibrium
between
colorless
lactone
and
zwitterion.
Tuning
the
lactone–zwitterion
constant
(KL–Z)
can
optimize
dye
properties
specific
biological
applications.
Here,
we
use
known
novel
organic
chemistry
to
prepare
comprehensive
collection
rhodamine
elucidate
structure–activity
relationships
that
govern
KL–Z.
We
discovered
auxochrome
substituent
strongly
affects
equilibrium,
providing
roadmap
rational
design
improved
dyes.
Electron-donating
auxochromes,
such
as
julolidine,
work
in
tandem
with
fluorinated
pendant
phenyl
rings
yield
bright,
red-shifted
fluorophores
live-cell
single-particle
tracking
(SPT)
multicolor
imaging.
The
N-aryl
combined
fluorination
yields
Förster
resonance
energy
transfer
(FRET)
quencher
useful
creating
new
semisynthetic
indicator
sense
cAMP
using
fluorescence
lifetime
imaging
microscopy
(FLIM).
Together,
this
expands
synthetic
methods
available
synthesis,
generates
reagents
advanced
experiments,
describes
will
guide
future
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
63(11)
Опубликована: Дек. 12, 2023
Abstract
Organic
fluorophores
are
indispensable
tools
in
cells,
tissue
and
vivo
imaging,
have
enabled
much
progress
the
wide
range
of
biological
biomedical
fields.
However,
many
available
dyes
suffer
from
insufficient
performances,
such
as
short
absorption
emission
wavelength,
low
brightness,
poor
stability,
small
Stokes
shift,
unsuitable
permeability,
restricting
their
application
advanced
imaging
technology
complex
imaging.
Over
past
two
decades,
efforts
been
made
to
improve
these
performances
fluorophores.
Starting
with
luminescence
principle
fluorophores,
this
review
clarifies
mechanisms
performance
for
traditional
a
certain
extent,
systematically
summarizes
modified
approaches
optimizing
properties,
highlights
typical
applications
improved
sensing,
indicates
existing
problems
challenges
area.
This
not
only
proves
significance
improving
but
also
provide
theoretical
guidance
development
high‐performance
Chemical Society Reviews,
Год журнала:
2023,
Номер
52(18), С. 6344 - 6358
Опубликована: Янв. 1, 2023
This
review
analyzes
strategies
to
convert
key
fluorophores
into
red/NIR
emitting
derivatives,
with
typical
examples
of
how
such
can
be
used
develop
molecular
probes
for
biological
analytes,
along
sensing
features.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Март 22, 2024
Abstract
Long-wavelength,
near-infrared
small-molecule
dyes
are
attractive
in
biophotonics.
Conventionally,
they
rely
on
expanded
aromatic
structures
for
redshift,
which
comes
at
the
cost
of
application
performance
such
as
photostability,
cell
permeability,
and
functionality.
Here,
we
report
a
ground-state
antiaromatic
strategy
showcase
concise
synthesis
14
cationic
aminofluorene
with
mini
(molecular
weights:
299–504
Da)
distinct
spectra
covering
700–1600
nm.
Aminofluorene
cell-permeable
achieve
rapid
renal
clearance
via
simple
44
Da
carboxylation.
This
accelerates
optical
diagnostics
injury
by
50
min
compared
to
existing
macromolecular
approaches.
We
develop
compact
molecular
sensing
platform
vivo
intracellular
sensing,
demonstrate
versatile
applications
these
multispectral
fluorescence
optoacoustic
imaging.
find
that
aromaticity
reversal
upon
electronic
excitation,
indicated
magnetic
descriptors,
not
only
reduces
energy
bandgap
but
also
induces
strong
vibronic
coupling,
resulting
ultrafast
excited-state
dynamics
unparalleled
photostability.
These
results
support
argument
antiaromaticity
useful
design
rule
dye
development,
enabling
performances
essential
modern
Chemical Society Reviews,
Год журнала:
2024,
Номер
53(13), С. 6992 - 7090
Опубликована: Янв. 1, 2024
Globally,
91%
of
plant
production
encounters
diverse
environmental
stresses.
Fluorescent
chemosensors
are
effective
for
monitoring
health
and
environment
that
promotes
the
development
sustainable
agriculture.
Nature Communications,
Год журнала:
2022,
Номер
13(1)
Опубликована: Янв. 14, 2022
Abstract
Modern
light
microscopy,
including
super-resolution
techniques,
has
brought
about
a
demand
for
small
labeling
tags
that
bring
the
fluorophore
closer
to
target.
This
challenge
can
be
addressed
by
unnatural
amino
acids
(UAAs)
with
bioorthogonal
click
chemistry.
The
minimal
size
of
UAA
and
possibility
couple
fluorophores
directly
protein
interest
single-residue
precision
in
living
cells
make
unique.
Here,
we
establish
primary
neurons
use
it
fixed-cell,
live-cell,
dual-color
pulse–chase,
microscopy
neurofilament
chain
(NFL).
We
also
show
combined
CRISPR/Cas9
genome
engineering
tagging
endogenous
NFL.
Due
its
versatile
nature
compatibility
advanced
multicolor
anticipate
will
contribute
novel
discoveries
neurobiology
field.
