bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 23, 2024
Abstract
Methylglyoxal
(MG)
is
an
endogenously
produced
non-enzymatic
side
product
of
glycolysis
that
acts
as
a
partial
agonist
at
GABA
A
receptors.
MG
metabolized
by
the
enzyme
glyoxalase-1
(GLO1).
Inhibition
GLO1
increases
methylglyoxal
levels,
and
has
been
shown
to
modulate
various
behaviors,
including
decreasing
seeking
cocaine-paired
cues
ethanol
consumption.
The
goal
these
studies
was
determine
if
inhibition
could
alter
cocaine-or
oxycodone-induced
locomotor
activation
and/or
conditioned
place
preference
(CPP)
cocaine
or
oxycodone.
We
used
both
pharmacological
genetic
manipulations
address
this
question.
Administration
inhibitor
s-bromobenzylglutathione
cyclopentyl
diester
(pBBG)
did
not
response
Additionally,
pBBG
had
no
significant
effect
on
for
Genetic
knockdown
Glo1
,
which
conceptually
similar
inhibition,
have
any
effects
preference,
nor
overexpression
affect
cocaine.
In
summary,
our
results
show
neither
influence
CPP
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 13, 2024
The
basolateral
amygdala
(BLA)
is
essential
for
assigning
positive
or
negative
valence
to
sensory
stimuli.
Noxious
stimuli
that
cause
pain
are
encoded
by
an
ensemble
of
Complex Psychiatry,
Год журнала:
2025,
Номер
11(1), С. 12 - 36
Опубликована: Март 3, 2025
Background:
Substance
use
disorder
(SUD)
is
closely
associated
with
epigenetic
modifications
that
significantly
impact
mental
health
outcomes.
Alcohol
and
drug
misuse
induce
widespread
changes
in
the
epigenome
transcriptome
of
central
nervous
system,
disrupting
critical
processes
such
as
reward
signaling
emotional
regulation.
These
alterations
regulation
gene
expression
often
persist
even
after
substance
cessation,
potentially
contributing
to
onset
or
worsening
psychiatric
conditions,
including
schizophrenia,
depression,
stress,
anxiety.
Summary:
This
review
delves
into
key
mechanisms
underlying
SUD
its
comorbid
disorders,
a
focus
on
DNA
methylation,
histone
modifications,
noncoding
RNA
Additionally,
it
examines
influence
environmental
biological
factors
evaluates
emerging
epigenetic-based
therapeutic
strategies
aimed
at
treating
related
conditions.
Key
Messages:
Gaining
deeper
understanding
driving
disorders
crucial
for
development
effective
interventions.
highlights
potential
pharmacological
mitigate
societal
personal
burdens
linked
complications.
Pharmaceuticals,
Год журнала:
2024,
Номер
17(5), С. 615 - 615
Опубликована: Май 10, 2024
The
relapse
rate
of
substance
abusers
is
high,
and
addiction
rehabilitation
adjunct
drugs
need
to
be
developed
urgently.
There
have
been
numerous
reports
on
blocking
the
formation
addiction,
but
studies
that
can
alleviate
withdrawal
symptoms
are
very
limited.
Both
dopamine
transporter
(DAT)
hypothesis
D3
receptor
(D3R)
proposed.
DAT
activators
reduce
extracellular
level,
D3R
antagonists
neuron’s
sensitivity
dopamine,
both
which
may
exacerbate
subsequently.
partial
agonist
SK608
has
biased
signaling
properties
via
G-protein-dependent
pathway
did
not
induce
desensitization
and,
thus,
a
promising
drug
for
symptoms.
Drugs
serotoninergic
neurons
or
GABAergic
anti-inflammatory
auxiliary
effects
treatments.
promote
structural
synaptic
plasticity
also
discussed.
Future Pharmacology,
Год журнала:
2024,
Номер
4(1), С. 115 - 138
Опубликована: Янв. 29, 2024
Addiction
is
a
complex
brain
disease
influenced
by
genetic,
environmental,
and
neurological
factors.
Psychostimulants,
cocaine,
methamphetamine
influence
different
cell
types
in
regions,
with
focus
on
the
neurons
responsible
for
rewarding
effects
nucleus
accumbens
(NAc)
ventral
tegmental
area
(VTA).
Known
markers
psychostimulant-induced
neuronal
plasticity
combination
droplet-based
high-throughput
single-cell
sequencing
divided
heterogeneity
of
populations
NAc
VTA
into
clusters,
where
all
cells
same
type
do
not
respond
equally
to
exposure
psychostimulants.
To
explain
as
changes
amplitude
phase
shifts
gene
expression,
we
focused
epigenetic
mechanisms
DNA
chromatin
modifications,
well
accessibility.
We
also
comment
epitranscriptomics
novel
approach
study
messenger
RNA
posttranslational
modification,
which
regulates
translation
potentially
localized
transcription
synapses
order
address
molecular
chains
that
connect
addiction
from
expression
synaptic
and,
finally,
plasticity.
Genome Research,
Год журнала:
2024,
Номер
34(11), С. 2081 - 2093
Опубликована: Ноя. 1, 2024
We
report
the
construction
and
analysis
of
a
new
reference
genome
assembly
for
Rattus
norvegicus
,
laboratory
rat,
widely
used
experimental
animal
model
organism.
The
has
been
adopted
as
rat
by
Genome
Reference
Consortium
is
named
GRCr8.
employed
40×
Pacific
Biosciences
(PacBio)
HiFi
sequencing
coverage
scaffolding
using
optical
mapping
Hi-C.
genomic
DNA
from
male
BN/NHsdMcwi
(BN)
same
strain
colony
prior
assembly,
mRatBN7.2.
at
chromosome
level
with
98.7%
sequence
assigned
to
chromosomes.
All
chromosomes
have
increased
in
size
compared
k
-mer
indicates
that
subject
fully
inbred
represented
single
haploid
assembly.
Notable
increases
are
observed
Chromosomes
3,
11,
12
prospective
rDNA
regions.
In
addition,
Chr
Y
threefold
more
consistent
karyotype
than
previous
assemblies.
Several
other
grown
incorporation
sizable
discrete
blocks.
These
contain
highly
repetitive
sequences
encode
numerous
previously
unannotated
genes.
centromeric
incorporated
most
annotation
performed
NCBI
RefSeq,
which
confirms
improvement
quality
adds
1100
protein
coding
PacBio
Iso-Seq
data
acquired
multiple
tissues
released
concurrently
aid
further
analyses.
