WOOLLY, interacting with MYB transcription factor MYB31, regulates cuticular wax biosynthesis by modulating CER6 expression in tomato

The Plant Journal, Год журнала: 2020, Номер 103(1), С. 323 - 337

Опубликована: Март 4, 2020

Cuticular waxes play a crucial role not only in plant defense against biotic and abiotic stresses, but also the quality storability of fruits, such as tomato (Solanum lycopersicum). Although biosynthetic pathways have been extensively characterized, regulatory mechanisms underlying wax biosynthesis remain largely unclear. Here, we show that Woolly (Wo), multicellular trichome regulator, is involved modulating tomato. Wo enhances expression genes SlCER6, SlKCR1, SlPAS2, transporter gene SlLTP, thereby promotes accumulation. Furthermore, directly binds to L1-box promoter an essential element very-long-chain fatty acid elongase complex. Intriguingly, overexpression (OE) or knock-down SlMYB31, MYB transcription factor physically interacts with vivo vitro, produces marked changes composition, whereas inhibits accumulation SlMYB31-OE lines, SlMYB31 Wo-OE implying these two function same pathway. Lastly, SlCER6 induced by abscisic manner partially dependent on Wo. These results demonstrate cooperatively control cuticular regulating SlCER6.

Язык: Английский

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Decoding the intermolecular recognition mode of a potent anticancer drug, abiraterone with human serum albumin: Assessments through spectroscopic and computational techniques

Journal of Molecular Structure, Год журнала: 2024, Номер 1302, С. 137509 - 137509

Опубликована: Янв. 5, 2024

Язык: Английский

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Systematic analysis of the non-specific lipid transfer protein gene family in Nicotiana tabacum reveal its potential roles in stress responses

Plant Physiology and Biochemistry, Год журнала: 2022, Номер 172, С. 33 - 47

Опубликована: Янв. 5, 2022

Язык: Английский

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Tomato Allergy: The Characterization of the Selected Allergens and Antioxidants of Tomato (Solanum lycopersicum)—A Review

Antioxidants, Год журнала: 2022, Номер 11(4), С. 644 - 644

Опубликована: Март 28, 2022

Tomatoes are one of the most broadly produced and consumed crop plants. They source health-promoting nutrients such as antioxidants, including ascorbic acid, polyphenols, or carotenoids. Despite beneficial role tomatoes in daily diet, they have been confirmed prevalent allergenic vegetables. Food allergies can cause many clinical symptoms, e.g., gastrointestinal tract, skin, lungs, well anaphylactic shock. A huge amount research has carried out to improve understanding immunological mechanisms that lead lack tolerance food antigens, which result either immunoglobulin E (IgE)-mediated reactions non-IgE-mediated reactions. Lifestyle diet play an important triggering allergies. Allergy is also linked other allergies, grass pollen latex allergy. Numerous attempts made identify characterize tomato allergens; however, data available on subject not sufficient.

Язык: Английский

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Lipid Transfer Proteins (LTPs)—Structure, Diversity and Roles beyond Antimicrobial Activity

Antibiotics, Год журнала: 2021, Номер 10(11), С. 1281 - 1281

Опубликована: Окт. 21, 2021

Lipid transfer proteins (LTPs) are among the most promising plant-exclusive antimicrobial peptides (AMPs). They figure challenging AMPs from point of view their structural diversity, functions and biotechnological applications. This review presents a current picture LTP research, addressing not only structural, evolutionary further predicted functional aspects. Traditionally, LTPs have been identified by direct isolation biochemical techniques, whereas omics data bioinformatics deserve special attention for potential to bring new insights. In this context, possible revealing that actually multipurpose, with many additional roles. Despite some challenges due toxicity allergenicity LTPs, systematic search in patent databases, indicate perspectives use human health also plant defense.

Язык: Английский

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Genome-Wide Analysis of nsLTP Gene Family and Identification of SiLTPs Contributing to High Oil Accumulation in Sesame (Sesamum indicum L.)

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(10), С. 5291 - 5291

Опубликована: Май 18, 2021

The biosynthesis and storage of lipids in oil crop seeds involve many gene families, such as nonspecific lipid-transfer proteins (nsLTPs). nsLTPs are cysteine-rich small basic essential for plant development survival. However, sesame, information related to was limited. Thus, the objectives this study were identify Sesamum indicum (SiLTPs) reveal their potential role accumulation sesame seeds. Genome-wide analysis revealed 52 SiLTPs, nonrandomly distributed on 10 chromosomes variety Zhongzhi 13. Following recent classification methods, SiLTPs divided into nine types, among which types I XI dominants. We found that could interact with several transcription factors, including APETALA2 (AP2), DNA binding one finger (Dof), etc. Transcriptome showed a tissue-specific expression some SiLTP genes. By integrating profiles weighted co-expression network (WGCNA) results two contrasting content varieties, we identified SiLTPI.23 SiLTPI.28 candidate genes high presumed functions validated through overexpression Arabidopsis thaliana. These findings expand our knowledge provide resources functional studies genetic improvement

Язык: Английский

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Effect of sprouting on the proteome of chickpea flour and on its digestibility by ex vivo gastro-duodenal digestion complemented with jejunal brush border membrane enzymes

Food Research International, Год журнала: 2022, Номер 154, С. 111012 - 111012

Опубликована: Фев. 16, 2022

Язык: Английский

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Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Biosensors, Год журнала: 2022, Номер 12(7), С. 503 - 503

Опубликована: Июль 9, 2022

Food allergy has been indicated as the most frequent adverse reaction to food ingredients over past few years. Since only way avoid occurrence of allergic phenomena is eliminate allergenic foods, it essential have complete and accurate information on components foodstuff. In this framework, mandatory crucial provide fast, cost-effective, affordable, reliable analysis methods for screening specific allergen content in products. This review reports research advancements concerning detection, involving electrochemical biosensors. It focuses sensing strategies evidencing different types recognition elements such antibodies, nucleic acids, cells, among others, nanomaterial role, several techniques involved last, but not least, ad hoc electrodic surface modification approaches. Moreover, a selection recent sensors detection are reported critically analyzed terms sensors’ analytical performances. Finally, advantages, limitations, potentialities practical applications biosensors allergens discussed.

Язык: Английский

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Genome-wide identification and evolutionary analysis of the AP2/EREBP, COX and LTP genes in Zea mays L. under drought stress

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Март 31, 2024

AP2 (APETALA2)/EREBP (ethylene-responsive element-binding protein), cytochrome c oxidase (COX) and nonspecific lipid transfer proteins (LTP) play important roles in the response to drought stress. This is first study identify COX gene Zea mays L. via genome-wide analysis. The qRT‒PCR results indicated that AP2/EREBP, LTP were downregulated, with fold changes of 0.84, 0.53 0.31, respectively, after 12 h Genome-wide analysis identified 78 6 10 genes Z. Domain confirmed presence domain, Cyt_c_Oxidase_Vb domain nsLTP1 proteins, respectively. AP2/EREBP protein family (AP2) includes five different types: AP2/ERF EREBP-like factor (EREBP), ethylene responsive (ERF), dehydration element binding (DREB) SHN SHINE. Synteny revealed collinearity orthologous relationships O. sativa, H. vulgare A. thaliana. found on chromosomes 1, 3, 4, 5, 7 8. 6, 8, 9 10. In present study, Ka/Ks ratios paralogous pairs influenced primarily by purifying selection, which received strong environmental pressure during evolution. COX-3/COX-4 indicate Darwinian selection (driving change). For genes, LTP-1/LTP-10, LTP-5/LTP-3 LTP-4/LTP-8 these while LTP-2/LTP-6 selection. duplication time ranged from approximately 9.364 100.935 Mya. pair was 5.217 19.064 96.477 major focus research are responsible for stress tolerance improve maize tolerance. will understanding functions

Язык: Английский

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Analysis of the molecular interaction of mitragynine from kratom with human α ₁ -acid glycoprotein: biophysical and molecular modeling investigations

Journal of Biomolecular Structure and Dynamics, Год журнала: 2025, Номер unknown, С. 1 - 17

Опубликована: Апрель 3, 2025

Mitragynine (MTG), the primary psychoactive alkaloid in Mitragyna speciosa (kratom), has garnered much attention for its therapeutic properties, which is attributed mainly to selective action on opioid receptors. Despite clinical potential, molecular framework of binding plasma proteins remains incomplete. Specifically, no studies have thoroughly examined interaction with α1-acid glycoprotein (AAG), a carrier protein circulatory system that influences drug disposition and bioavailability. Hence, this study aims explore dynamics between MTG AAG using combination spectroscopic, calorimetric, microscopic, computational methods. Based isothermal titration calorimetric fluorescence studies, an intermediate affinity MTG-AAG was determined (Ka ∼ 105 M-1). evidence microenvironmental changes around Trp residues, did not disrupt overall structural integrity AAG. Thermodynamic analysis indicated energetically favorable, enthalpy driven by hydrogen bonding van der Waals forces, negative entropy change suggesting more ordered complex formation. Docking showed embedded deeply within central cavity variant F1*S, enhancing stability, as opposed near entrance A. Molecular simulations supported stable complexation both variants, F1*S maintaining compactness while A exhibited slight unfolding upon binding. These findings clear significance potential applications kratom-derived drugs, especially those structurally related MTG.

Язык: Английский

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