Therapeutic targets and pharmacological mechanisms of Coptidis Rhizoma against ulcerative colitis: Findings of system pharmacology and bioinformatics analysis

Frontiers in Pharmacology, Год журнала: 2022, Номер 13

Опубликована: Ноя. 30, 2022

Evidence of the advantages Coptidis Rhizoma (CR) for treatment ulcerative colitis (UC) is accumulating. However, research revealing targets and molecular mechanisms CR against UC scarce. In this research, a bioinformatics analysis was performed to carry out physicochemical properties biological activities phytochemicals in analyze binding activities, targets, functions UC. This shows that CR’s key phytochemicals, which are named Coptisine, Berberrubine, Berlambine, Berberine, Epiberberine, Obacunone, Worenine, Quercetin, (R)-Canadine, Magnograndiolide, Palmatine Moupinamide, have ideal bioactivity. A total 1,904 potential phytochemical 17,995 UC-related identified, we finally acquire 233 intersection between disease. protein-protein interaction network common constructed; six hub were acquired with degree greater than or equal median, namely TP53, HSP90AA1, STAT3, ESR1, MYC, RELA. The enrichment suggested core may exert an impact on anti-inflammatory, immunoregulatory, anti-oxidant anti-fibrosis mainly through PI3K/ART signaling pathway, Th17 differentiation inflammatory bowel disease etcetera. Also, docking strong affinity targets. Finally, CR, GO functions, KEGG pathways constructed. study indicates superior drug likeness bioactivity, mechanism be via pathway mentioned above. critical pharmacological provide direction future research.

Язык: Английский

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Analysis and identification of potential type II helper T cell (Th2)-Related key genes and therapeutic agents for COVID-19

Computers in Biology and Medicine, Год журнала: 2022, Номер 150, С. 106134 - 106134

Опубликована: Сен. 22, 2022

Язык: Английский

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Bioinformatics-based analysis of the dialog between COVID-19 and RSA

Heliyon, Год журнала: 2024, Номер 10(9), С. e30371 - e30371

Опубликована: Апрель 27, 2024

Pregnant women infected with SARS-CoV-2 in early pregnancy may face an increased risk of miscarriage due to immune imbalance at the maternal-fetal interface. However, molecular mechanisms underlying crosstalk between COVID-19 infection and recurrent spontaneous abortion (RSA) remain poorly understood. This study aimed elucidate transcriptomic dialog RSA. Based on bioinformatics analysis, 307 common differentially expressed genes were found (GSE171110) RSA (GSE165004). Common DEGs mainly enriched ribosome-related cell cycle-related signaling pathways. Using degree algorithm, top 10 hub (RPS27A, RPL5, RPS8, RPL4, RPS2, RPL30, RPL23A, RPL31, RPL26, RPL37A) selected from based their scores. The results qPCR general agreement raw letter analysis. candidate drugs also P-values. In this study, we provide markers, pathways, small molecule compounds that associate COVID-19. These findings increase accurate diagnosis treatment patients.

Язык: Английский

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The interplay between severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) and the hypothalamic and endocrinal disorders: A Mini-review

Egyptian journal of Immunology, Год журнала: 2023, Номер 30(1), С. 73 - 86

Опубликована: Янв. 1, 2023

The worldwide medical systems are still being severely impacted by the coronavirus disease-2019 (COVID-19) pandemic, which is responsible for catastrophic mortality and morbidity. It becomes more obvious that this unique respiratory virus's impacts go beyond system as time goes on our comprehension of it deepens. transmembrane serine protease 2 (TMPRSS2) protein necessary severe acute syndrome 2, cause COVID-19, to gain cellular entry through angiotensin-converting enzyme (ACE2) receptor. Most endocrine glands exhibit high levels expression ACE2 TMPRSS2. This pays attention effect COVID-19 system. Besides its capability pass central nervous especially hypothalamus inducing a lot functional disorders in individuals. Although effective vaccines became widely available, declined but shifting lengthy health survivors. To inform suitable research management, review provides an overview data examining besides hypothalamus. In addition, we reported if endocrinal thalamic could affect incidence progress COVID-19.

Язык: Английский

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Unraveling the mechanism of action of cepharanthine for the treatment of novel coronavirus pneumonia (COVID-19) from the perspectives of systematic pharmacology

Arabian Journal of Chemistry, Год журнала: 2023, Номер 16(6), С. 104722 - 104722

Опубликована: Март 6, 2023

Natural products play an irreplaceable role in the treatment of SARS-CoV-2 infection. Nevertheless, underlying molecular mechanisms involved remain elusive. To better understand their potential therapeutic effects, more validation studies are needed to explore systematically. This study aims targets action and signaling pathways cepharanthine for COVID-19. revealed that a total 173 Cepharanthine 86 intersectional against COVID-19 were screened collected. Gene Ontology enrichment analysis suggested inflammatory, immune cell enzyme activities critical terms Pathway showed five associated with main via cepharanthine. Molecular docking dynamics simulations 6 core regarded as In brief, demonstrates may important infection through its harmonious activity multiple related targets. article provides valuable insights required respond effectively concerns western medical community.

Язык: Английский

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Age- and sex-specific differences in immune responses to BNT162b2 COVID-19 and live-attenuated influenza vaccines in UK adolescents

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Окт. 6, 2023

Introduction The key to understanding the COVID-19 correlates of protection is assessing vaccine-induced immunity in different demographic groups. Young people are at a lower risk mortality, females than males, and often generate stronger immune responses vaccination. Methods We studied two doses BNT162b2 Pfizer vaccine an adolescent cohort (n = 34, ages 12–16), age group previously shown elicit significantly greater same young adults. Adolescents were with aim comparing their response that adults; assess impacts other factors such as sex, ongoing SARS–CoV–2 infection schools, prior exposure endemic coronaviruses circulate high levels people. At time, we able evaluate co-administered live attenuated influenza vaccine. Blood samples from 34 adolescents taken before after vaccination vaccines assayed for SARS–CoV–2-specific IgG neutralising antibodies cellular specific betacoronaviruses. targeting lineages contained also assessed. Results Robust identified infected one dose, required infection-naïve adolescents. As demonstrated, total SARS–CoV-2 Spike higher among vaccinated adults (aged 32–52) who received (comparing infection-naïve, 49,696 vs. 33,339; p 0.03; SARS-CoV–2 infected, 743,691 269,985; <0.0001) by MSD v-plex assay. There was no evidence compared males. Discussion These findings may result introduction novel mRNA platforms, generating patterns divergent established trends providing new insights into what might be protective following

Язык: Английский

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HGCA2.0: An RNA-Seq Based Webtool for Gene Coexpression Analysis in Homo sapiens

Cells, Год журнала: 2023, Номер 12(3), С. 388 - 388

Опубликована: Янв. 21, 2023

Genes with similar expression patterns in a set of diverse samples may be considered coexpressed. Human Gene Coexpression Analysis 2.0 (HGCA2.0) is webtool which studies the global coexpression landscape human genes. The website based on hierarchical clustering 55,431 Homo sapiens genes large-scale analysis 3500 GTEx bulk RNA-Seq healthy individuals, were selected as best representative each tissue type. HGCA2.0 presents subclades coexpressed to gene interest, and performs various built-in term enrichment analyses genes, including ontologies, biological pathways, protein families, diseases, while also being unique revealing enriched transcription factors driving coexpression. has been successful identifying not only ubiquitous patterns, but tissue-specific Benchmarking showed that belongs top performing webtools, shown by STRING analysis. creates working hypotheses for discovery partners or common processes can experimentally validated. It offers simple intuitive design user interface, well an API endpoint.

Язык: Английский

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Deciphering the potential therapeutic targets and mechanisms of jaranol for the treatment of COVID-19 and lung adenocarcinoma

Arabian Journal of Chemistry, Год журнала: 2024, Номер 17(3), С. 105648 - 105648

Опубликована: Янв. 24, 2024

Patients with lung adenocarcinoma (LUAD) are vulnerable to COVID-19. However, there is currently a lack of available treatments for LUAD patients infected Jaranol naturally derived flavonoid that exhibits promising properties as an antiviral and antitumor agent. Therefore, this research intends investigate the molecular targets underlying mechanisms jaranol treatment COVID-19/LUAD. Through network pharmacology, 47 intersection target genes were identified against COVID-19/LUAD, eight-gene risk score model strong predictive accuracy was constructed. Functional enrichment analysis demonstrated main mechanism in combating COVID-19/LUAD involved regulation response hormone, positive phosphorylation, PI3K-Akt signaling pathway. Furthermore, results docking indicated exhibited significant affinity eight hub (AKT1, SRC, EGFR, HSP90AA1, ESR1, PTGS2, PPARG, MMP9) well three core COVID-19-related (ACE2, 3CLpro, PLpro). The direct binding between MMP9 then validated A549 cancer cells using cellular thermal shift assay (CETSA). These findings suggest has therapeutic effect providing theoretical foundation novel perspectives future development potential pharmaceutical candidate.

Язык: Английский

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Loss of estrogen unleashing neuro-inflammation increases the risk of Alzheimer’s disease in women

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Сен. 20, 2022

Abstract The risk of Alzheimer’s disease (AD) in women is about 2 times greater than men. estrogen hypothesis being accepted as the essential sex factor causing difference AD. Also, recent meta-analysis using large-scale medical records data indicated replacement therapy. However, underlying molecular targets and mechanisms explaining this AD development remain unclear. In study, we identified that treatment can strongly inhibition neuro-inflammation signaling targets, systems pharmacology model; ESR1/ESR2 (the receptors estrogen) are topologically close to neuroinflammation biomarker genes network analysis. Moreover, level decreased an extremely lower men after age 55. Pooling together multiple pieces evidence, it concluded loss unleashing increases women’s disease. These analysis results provide novel supporting evidence potential mechanism anti-neuroinflammation role Medications boosting direct downstream ESR1/ESR2, or inhibiting upstream neuroinflammation, like JAK2 inhibitors, on be potentially effective synergistic combined with for prevention treatment.

Язык: Английский

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Biofunctional roles of estrogen in coronavirus disease 2019: Beyond a steroid hormone

Frontiers in Pharmacology, Год журнала: 2022, Номер 13

Опубликована: Окт. 19, 2022

The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome 2 (SARS-CoV-2), epidemic poses a major global public health threat with more than one million daily new infections and hundreds of deaths. To combat this pandemic, efficient prevention management strategies are urgently needed. Together the main characteristics COVID-19, impaired coagulation dysfunctions immune response in COVID-19 pathophysiology causes high mortality morbidity. From recent clinical observations, increased expression specific types estrogen appears to protect patients from SARS-CoV-2 infection, thereby, reducing mortality. severity is less common women men, particularly menopausal women. Furthermore, levels negatively correlated These findings suggest that plays protective role COVID-19. In review, we discuss potential roles blocking invading alveolar cells replicating, summarize mechanisms anti-inflammation, modulation, reactive oxygen species resistance, anti-thrombosis, vascular dilation, endothelium protection. Finally, therapeutic effects against reviewed. This review provides insights into its use as strategy reduce associated possibly other viral discusses possible challenges pertinent questions.

Язык: Английский

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