Ageing Research Reviews, Год журнала: 2024, Номер 101, С. 102530 - 102530
Опубликована: Окт. 11, 2024
Язык: Английский
Human Reproduction Update, Год журнала: 2024, Номер 30(5), С. 614 - 647
Опубликована: Июнь 28, 2024
Chemotherapy-associated ovarian damage (CAOD) is one of the most feared short- and long-term side effects anticancer treatment in premenopausal women. Accumulating detailed data show that different chemotherapy regimens can lead to disturbance hormone levels, reduced or lost fertility, an increased risk early menopause. Previous studies have often focused on direct chemotherapeutic drugs follicles, such as DNA damage-mediated apoptotic death primordial follicle burnout. Emerging evidence has revealed imbalance microenvironment during chemotherapy. The provides nutritional support transportation signals stimulate growth development ovulation, corpus luteum formation. close interaction between follicles determine function. Therefore, designing novel precise strategies manipulate may be a new strategy protect function
Язык: Английский
Процитировано
14
Nature Reviews Molecular Cell Biology, Год журнала: 2024, Номер 25(12), С. 979 - 1000
Опубликована: Окт. 3, 2024
Язык: Английский
Процитировано
8
Small Methods, Год журнала: 2025, Номер unknown
Опубликована: Янв. 28, 2025
Abstract Accurately defining cell boundaries for spatial transcriptomics is technically challenging. The current major approaches are nuclear staining or mathematical inference, which either exclude the cytoplasm determine a hypothetical boundary. Here, new method introduced boundaries: labeling membranes using genetically coded fluorescent proteins, allows precise indexing of sequencing spots and transcripts within cells on sections. Use this membrane‐based greatly increases number genes captured in compared to nucleus‐based methods; numbers increased by 67% 119% mouse axolotl livers, respectively. obtained expression profiles more consistent with single‐cell RNA‐seq data, demonstrating rational clustering apparent type‐specific markers. Furthermore, improved resolution achieved better identify rare types elaborate domains brain intestine. In addition regular cells, accurate recognition multinucleated lacking nuclei liver achieved, its ability analyze complex tissues organs, not achievable previous methods. This study provides powerful tool improving that has broad potential applications biological medical sciences.
Язык: Английский
Процитировано
1
Trends in Endocrinology and Metabolism, Год журнала: 2024, Номер unknown
Опубликована: Авг. 1, 2024
Язык: Английский
Процитировано
5
Redox Biology, Год журнала: 2025, Номер 81, С. 103584 - 103584
Опубликована: Март 7, 2025
Ovarian aging typically precedes the decline of other organ systems, yet its molecular mechanisms remain poorly understood. Glycosylation as one most important protein modifications has been especially unexplored in this context. Here, we present first high-resolution glycoproteomic landscape mouse ovaries, uncovering site-specific N-glycan signatures across subcellular components such high proportions complex glycans, core fucosylation, and LacdiNAc branches at zone pellucida. We report three major glycosylation alterations aged ovaries: frequently changed core-fucosylation associated with cell adhesion immune responses, decreased glycans on zona pellucida (ZP) responsible for fertility decline, increased sialylated modified by Neu5Ac Neu5Gc playing different roles activation responses. Integrated multi-omic analyses further highlight unique role glycosylation, distinct from phosphorylation, regulating key signaling pathways, antigen processing presentation, complement coagulation cascades, ROS biosynthetic metabolic processes, well death. This study offers a novel glycobiological perspective ovarian aging, broadening our understanding beyond traditional approaches.
Язык: Английский
Процитировано
0
Aging Cell, Год журнала: 2025, Номер unknown
Опубликована: Янв. 2, 2025
ABSTRACT With advancing age, significant changes occur in the female reproductive system, most notable of which is decline oocyte quality, a key factor affecting fertility. However, mechanisms underlying aging remain poorly understood. In this study, we obtained oocytes from aged and young mice performed single‐cell transcriptome sequencing, comparing our findings with existing proteomic analyses. Our analysis revealed that one primary characteristics disruption calcium ion homeostasis. Specifically, identified two genes involved process, Calb1 Rpl23 . Experimental validation demonstrated knockdown CALB1 led to reduced levels endoplasmic reticulum mitochondria, resulting mitochondrial dysfunction meiotic defects. Further experiments suggested RPL23 may function as downstream gene CALB1, its caused dysfunction, excessive accumulation reactive oxygen species (ROS), spindle assembly Notably, overexpression these partially rescued maternal age‐related defective phenotypes, underscoring their crucial roles aging. This study provides comprehensive understanding specific mouse at resolution, supported by experimental validation, offers new directions potential targets for future research into health issues.
Язык: Английский
Процитировано
0
Life Medicine, Год журнала: 2025, Номер 4(1)
Опубликована: Янв. 23, 2025
The ovary is a crucial gonadal organ that supports female reproductive and endocrine functions. Ovarian aging can result in decreased fertility dysfunction across multiple organs. Research has demonstrated cellular senescence various cell types within the trigger decline ovarian function through distinct stress responses, resulting aging. This review explores how may contribute to failure. Additionally, we discuss factors cause senescence, including accumulation of advanced glycation end products, oxidative stress, mitochondrial dysfunction, DNA damage, telomere shortening, exposure chemotherapy. Furthermore, six types, oocytes, granulosa cells, theca immune surface epithelium, endothelial inside explore their contribution accelerated Lastly, describe potential senotherapeutics for treatment offer novel strategies longevity.
Язык: Английский
Процитировано
0
GeroScience, Год журнала: 2025, Номер unknown
Опубликована: Фев. 11, 2025
Язык: Английский
Процитировано
0
Human Reproduction Update, Год журнала: 2025, Номер unknown
Опубликована: Фев. 20, 2025
Ovarian aging occurs earlier than the of many other organs and has a lasting impact on women's overall health well-being. However, effective interventions to slow ovarian remain limited, primarily due an incomplete understanding underlying molecular mechanisms drug targets. Recent advances in omics data resources, combined with innovative computational tools, are offering deeper insight into complexities aging, paving way for new opportunities discovery development. This review aims synthesize expanding multi-omics data, spanning genome, transcriptome, proteome, metabolome, microbiome, related from both tissue-level single-cell perspectives. We will specially explore how analysis these emerging datasets can be leveraged identify novel targets guide therapeutic strategies slowing reversing aging. conducted comprehensive literature search PubMed database using range relevant keywords: age at natural menopause, premature insufficiency (POI), diminished reserve (DOR), genomics, transcriptomics, epigenomics, DNA methylation, RNA modification, histone proteomics, metabolomics, lipidomics, single-cell, genome-wide association studies (GWAS), whole-exome sequencing, phenome-wide (PheWAS), Mendelian randomization (MR), epigenetic target, machine learning, artificial intelligence (AI), deep multi-omics. The was restricted English-language articles published up September 2024. Multi-omics have uncovered key driving including damage repair deficiencies, inflammatory immune responses, mitochondrial dysfunction, cell death. By integrating researchers critical regulatory factors across various biological levels, leading potential Notable examples include genetic such as BRCA2 TERT, like Tet FTO, metabolic sirtuins CD38+, protein BIN2 PDGF-BB, transcription FOXP1. advent cutting-edge technologies, especially technologies spatial provided valuable insights guiding treatment decisions become powerful tool aimed mitigating or As technology advances, integration AI models holds more accurately predict candidate convergence offers promising avenues personalized medicine precision therapies, tailored Not applicable.
Язык: Английский
Процитировано
0
Trends in Endocrinology and Metabolism, Год журнала: 2025, Номер unknown
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0