Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action

BMC Genomics, Год журнала: 2018, Номер 19(1)

Опубликована: Дек. 1, 2018

Epigenetic modifications including DNA methylation and post-translational of histones are known to regulate gene expression. Antagonistic activities histone acetyltransferases (HATs) deacetylases (HDACs) mediate transcriptional reprogramming during insect development as shown in Drosophila melanogaster other insects. Juvenile hormones (JH) play vital roles the regulation growth, development, metamorphosis, reproduction physiological processes. However, our current understanding epigenetic JH action is still limited. Hence, we studied role CREB binding protein (CBP, contains HAT domain) Trichostatin A (TSA, HDAC inhibitor) on action.

Язык: Английский

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BgFas1: A fatty acid synthase gene required for both hydrocarbon and cuticular fatty acid biosynthesis in the German cockroach, Blattella germanica (L.)

Insect Biochemistry and Molecular Biology, Год журнала: 2019, Номер 112, С. 103203 - 103203

Опубликована: Авг. 16, 2019

Язык: Английский

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Circadian clock genes link photoperiodic signals to lipid accumulation during diapause preparation in the diapause-destined female cabbage beetles Colaphellus bowringi

Insect Biochemistry and Molecular Biology, Год журнала: 2018, Номер 104, С. 1 - 10

Опубликована: Ноя. 10, 2018

Язык: Английский

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Transcriptome and physiological analysis reveal alterations in muscle metabolisms and immune responses of grass carp (Ctenopharyngodon idellus) cultured at different stocking densities

Aquaculture, Год журнала: 2019, Номер 503, С. 186 - 197

Опубликована: Янв. 6, 2019

Язык: Английский

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The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, Spodoptera litura (Lepidoptera: Noctuidae)

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(16), С. 9064 - 9064

Опубликована: Авг. 13, 2022

Fatty acid synthase (FAS) is a key enzyme in the lipid synthesis pathway, however, its roles insects remain largely unknown. Here, we firstly identified two FAS genes from transcriptome dataset of general cutworm Spodoptera litura, which destructive insect pest many crops. Both SlFAS1 and SlFAS2 were highly expressed third instar larvae their fat bodies. Then, successfully silenced content α-linolenic triglyceride was significantly decreased. Besides that, effect on metamorphic development S. litura evaluated. The results indicate that after silencing SlFAS1, survival rates decreased compared to control groups. Silencing fifth resulted more malformed pupae adults, emergence reduced. Furthermore, ecdysone haemolymph SlFAS1. In addition, knocking down alters expression other lipogenesis implying has an impact pathway. present study deepens understanding provides novel potential targets for managing pests.

Язык: Английский

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A fatty acid synthase gene (FASN3) from the integument tissue of Rhodnius prolixus contributes to cuticle water loss regulation

Insect Molecular Biology, Год журнала: 2019, Номер 28(6), С. 850 - 861

Опубликована: Май 24, 2019

Abstract Fatty acid synthase is a multifunctional enzyme involved in the formation of fatty acids. Despite role acids cell signalling and energy metabolism, as precursors to pheromones hydrocarbons that waterproof cuticle, insect synthases have been scarcely studied. Here we perform molecular characterization three genes ( RPRC000123 , RPRC000269 RPRC002909 ) Chagas disease vector, Rhodnius prolixus . Gene expression screening by reverse transcription quantitative PCR showed are expressed almost exclusively integument tissue whilst mostly fat body also several organs. Phylogenetic analysis, together with gene results, orthologues Drosophila melanogaster 1 FASN1 ), FASN2 FASN3 genes, respectively. After RNA interference‐mediated knockdown insects died immediately after moulting next developmental stage. However, mortality was prevented placing under saturated humidity conditions, suggesting dehydration might play insects’ death. Lipid analyses ‐silenced reduced amounts methyl‐branched hydrocarbons, compared controls. These data support an important for biosynthesis cuticle.

Язык: Английский

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Multiple functions of CREB-binding protein during postembryonic development: identification of target genes

BMC Genomics, Год журнала: 2017, Номер 18(1)

Опубликована: Дек. 1, 2017

Juvenile hormones (JH) and ecdysteroids control postembryonic development in insects. They serve as valuable targets for pest management. Hence, understanding the molecular mechanisms of their action is crucial importance. CREB-binding protein (CBP) a universal transcriptional co-regulator. It controls expression several genes including those from hormone signaling pathways through co-activation many transcription factors. However, role CBP during insects not well understood. Therefore, we have studied Tribolium, model coleopteran insect. ubiquitously expressed red flour beetle, Tribolium castaneum. RNA interference (RNAi) mediated knockdown resulted decrease JH induction Kr-h1 gene larvae led to block development. Moreover, injection double-stranded (dsRNA) showed lethal phenotypes within 8 days injection. RNA-seq subsequent differential analysis identified target Tribolium. Knockdown caused 1306 coding factors other proteins associated with growth Depletion impaired response (e.g., Kr-h1, Hairy, early trypsin) ecdysone (EcR, E74, E75, broad complex). Further, GO enrichment analyses downregulated different functions developmental processes, pigmentation, anatomical structure development, regulation biological cellular etc. These data suggest diverse but roles insect, can RNAi management this stored product pest.

Язык: Английский

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Reference genes validation in Phenacoccus solenopsis under various biotic and abiotic stress conditions

Scientific Reports, Год журнала: 2017, Номер 7(1)

Опубликована: Окт. 13, 2017

Real-time PCR (RT-qPCR) expression analysis is a powerful analytical technique, but for normalization of data requires the use stable reference genes. However, suitable genes are still not known in case Phenacoccus solenopsis under variable experimental treatments. The present study focused on identification housekeeping as different abiotic and biotic factors P. solenopsis. We analyzed relative six commonly used candidate developmental stages, host-feeding assay, temperature treatments field distribution conditions. Expression stabilities were by geNorm, NormFinder, RefFinder. Under conditions, β-Tubulin was found to be most followed rpl32 α-Tubulin. In host feeding treatment α-tubulin identified genes, while stress, combination α-Tubulin normalizing RT-qPCR data. Further, above-identified validated using based gene four objective namely, Myoinhibitory peptides (MIPs), Zinc_metalloprotease (Zn_Mp), fatty acid synthase (fas) alpha-glucosidase. Identified will facilitate studies future stress

Язык: Английский

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Two fatty acid synthase genes from the integument contribute to cuticular hydrocarbon biosynthesis and cuticle permeability in Locusta migratoria

Insect Molecular Biology, Год журнала: 2020, Номер 29(6), С. 555 - 568

Опубликована: Авг. 2, 2020

Abstract Lipids of the insect cuticle have important roles in resistance against arid environment and invasion foreign substances. Fatty acid synthase (FAS) is an enzyme lipid synthesis pathway. In present study, we identified three FAS genes from transcriptome data migratory locust, Locusta migratoria , based on bioinformatics analyses. Among them, two ( LmFAS1 LmFAS3 ) are highly expressed integument fifth instar nymphs. Suppression by RNA interference caused lethality during ecdysis or shortly after moulting. The weight locusts content droplets were reduced compared with those control. results gas chromatography–mass spectrometry analysis showed that knockdown led to a decrease both cuticular hydrocarbons inner (CHCs IHCs) contents, especially methyl branched hydrocarbons. By contrast, only resulted IHC content, but not CHCs. consequence, LmFAS1‐ ‐suppressed locusts, hydrocarbon deficiency desiccation enhanced permeability sensitivity insecticides. These indicate essential for production permeability, which play influential waterproofing cuticle.

Язык: Английский

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Juvenile hormone biosynthetic genes are critical for regulating reproductive diapause in the cabbage beetle

Insect Biochemistry and Molecular Biology, Год журнала: 2021, Номер 139, С. 103654 - 103654

Опубликована: Сен. 24, 2021

Язык: Английский

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