Metabolic and Transcriptional Regulation of Reproductive Diapause in Arma chinensis

iScience, Journal Year: 2025, Volume and Issue: 28(3), P. 111761 - 111761

Published: Jan. 7, 2025

Diapause enables insects to survive unfavorable conditions through metabolic and developmental adjustments. We investigated regulation during reproductive diapause in the predatory stinkbug Arma chinensis using transcriptomic metabolomic analyses. Our study revealed 9,254 differentially expressed genes 493 significantly changed metabolites across stages. Key pathways including glutathione metabolism, TCA cycle, glycolysis, lipid metabolism underwent substantial reorganization. The pre-diapause phase showed increased energy consumption accumulation, while maintenance exhibited restructuring of amino acid glucose metabolism. identified stage-specific signatures potential regulatory mechanisms, roles redox insulin signaling control. This comprehensive characterization reprogramming A. provides insights for improving biocontrol agent production storage strategies.

Language: Английский

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Endocrine and enzymatic shifts during insect diapause: a review of regulatory mechanisms

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 16

Published: March 14, 2025

Insect diapause is a vital survival strategy that enables insects to enter state of suspended development, allowing them withstand unfavorable environmental conditions. During diapause, significantly lower their metabolic rate and build up energy reserves, which they gradually utilize throughout this period. The regulation involves complex interaction hormones enzymes. Juvenile (JHs) affect adults larvae differently; in adults, the absence JH typically triggers while larvae, presence encourages state. Ecdysteroids, regulate molting metamorphosis, are carefully controlled prevent premature development. Reduced signaling insulin-like peptides enhances stress resistance promotes storage. Several enzymes play crucial roles adjustments necessary for diapause. These include degradation JH, ecdysteroidogenic pathway, metabolism fatty acids, glycogen, cryoprotectants, responses. Understanding diapause's molecular biochemical mechanisms essential fundamental entomological research practical applications. Despite recent advances, many aspects regulation, especially interactions among hormonal pathways role enzymes, remain poorly understood. This review analyzes approximately 250 papers consolidate current knowledge on enzymatic It offers comprehensive overview key processes based studies suggests future directions fill gaps our understanding significant biological phenomenon. also lays groundwork enhancing pest control strategies ecological conservation by deepening mechanisms.

Language: Английский

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Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action

BMC Genomics, Journal Year: 2018, Volume and Issue: 19(1)

Published: Dec. 1, 2018

Epigenetic modifications including DNA methylation and post-translational of histones are known to regulate gene expression. Antagonistic activities histone acetyltransferases (HATs) deacetylases (HDACs) mediate transcriptional reprogramming during insect development as shown in Drosophila melanogaster other insects. Juvenile hormones (JH) play vital roles the regulation growth, development, metamorphosis, reproduction physiological processes. However, our current understanding epigenetic JH action is still limited. Hence, we studied role CREB binding protein (CBP, contains HAT domain) Trichostatin A (TSA, HDAC inhibitor) on action.

Language: Английский

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BgFas1: A fatty acid synthase gene required for both hydrocarbon and cuticular fatty acid biosynthesis in the German cockroach, Blattella germanica (L.)

Insect Biochemistry and Molecular Biology, Journal Year: 2019, Volume and Issue: 112, P. 103203 - 103203

Published: Aug. 16, 2019

Language: Английский

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Circadian clock genes link photoperiodic signals to lipid accumulation during diapause preparation in the diapause-destined female cabbage beetles Colaphellus bowringi

Insect Biochemistry and Molecular Biology, Journal Year: 2018, Volume and Issue: 104, P. 1 - 10

Published: Nov. 10, 2018

Language: Английский

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Transcriptome and physiological analysis reveal alterations in muscle metabolisms and immune responses of grass carp (Ctenopharyngodon idellus) cultured at different stocking densities

Aquaculture, Journal Year: 2019, Volume and Issue: 503, P. 186 - 197

Published: Jan. 6, 2019

Language: Английский

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The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, Spodoptera litura (Lepidoptera: Noctuidae)

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(16), P. 9064 - 9064

Published: Aug. 13, 2022

Fatty acid synthase (FAS) is a key enzyme in the lipid synthesis pathway, however, its roles insects remain largely unknown. Here, we firstly identified two FAS genes from transcriptome dataset of general cutworm Spodoptera litura, which destructive insect pest many crops. Both SlFAS1 and SlFAS2 were highly expressed third instar larvae their fat bodies. Then, successfully silenced content α-linolenic triglyceride was significantly decreased. Besides that, effect on metamorphic development S. litura evaluated. The results indicate that after silencing SlFAS1, survival rates decreased compared to control groups. Silencing fifth resulted more malformed pupae adults, emergence reduced. Furthermore, ecdysone haemolymph SlFAS1. In addition, knocking down alters expression other lipogenesis implying has an impact pathway. present study deepens understanding provides novel potential targets for managing pests.

Language: Английский

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A fatty acid synthase gene (FASN3) from the integument tissue of Rhodnius prolixus contributes to cuticle water loss regulation

Insect Molecular Biology, Journal Year: 2019, Volume and Issue: 28(6), P. 850 - 861

Published: May 24, 2019

Abstract Fatty acid synthase is a multifunctional enzyme involved in the formation of fatty acids. Despite role acids cell signalling and energy metabolism, as precursors to pheromones hydrocarbons that waterproof cuticle, insect synthases have been scarcely studied. Here we perform molecular characterization three genes ( RPRC000123 , RPRC000269 RPRC002909 ) Chagas disease vector, Rhodnius prolixus . Gene expression screening by reverse transcription quantitative PCR showed are expressed almost exclusively integument tissue whilst mostly fat body also several organs. Phylogenetic analysis, together with gene results, orthologues Drosophila melanogaster 1 FASN1 ), FASN2 FASN3 genes, respectively. After RNA interference‐mediated knockdown insects died immediately after moulting next developmental stage. However, mortality was prevented placing under saturated humidity conditions, suggesting dehydration might play insects’ death. Lipid analyses ‐silenced reduced amounts methyl‐branched hydrocarbons, compared controls. These data support an important for biosynthesis cuticle.

Language: Английский

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Multiple functions of CREB-binding protein during postembryonic development: identification of target genes

BMC Genomics, Journal Year: 2017, Volume and Issue: 18(1)

Published: Dec. 1, 2017

Juvenile hormones (JH) and ecdysteroids control postembryonic development in insects. They serve as valuable targets for pest management. Hence, understanding the molecular mechanisms of their action is crucial importance. CREB-binding protein (CBP) a universal transcriptional co-regulator. It controls expression several genes including those from hormone signaling pathways through co-activation many transcription factors. However, role CBP during insects not well understood. Therefore, we have studied Tribolium, model coleopteran insect. ubiquitously expressed red flour beetle, Tribolium castaneum. RNA interference (RNAi) mediated knockdown resulted decrease JH induction Kr-h1 gene larvae led to block development. Moreover, injection double-stranded (dsRNA) showed lethal phenotypes within 8 days injection. RNA-seq subsequent differential analysis identified target Tribolium. Knockdown caused 1306 coding factors other proteins associated with growth Depletion impaired response (e.g., Kr-h1, Hairy, early trypsin) ecdysone (EcR, E74, E75, broad complex). Further, GO enrichment analyses downregulated different functions developmental processes, pigmentation, anatomical structure development, regulation biological cellular etc. These data suggest diverse but roles insect, can RNAi management this stored product pest.

Language: Английский

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Reference genes validation in Phenacoccus solenopsis under various biotic and abiotic stress conditions

Scientific Reports, Journal Year: 2017, Volume and Issue: 7(1)

Published: Oct. 13, 2017

Real-time PCR (RT-qPCR) expression analysis is a powerful analytical technique, but for normalization of data requires the use stable reference genes. However, suitable genes are still not known in case Phenacoccus solenopsis under variable experimental treatments. The present study focused on identification housekeeping as different abiotic and biotic factors P. solenopsis. We analyzed relative six commonly used candidate developmental stages, host-feeding assay, temperature treatments field distribution conditions. Expression stabilities were by geNorm, NormFinder, RefFinder. Under conditions, β-Tubulin was found to be most followed rpl32 α-Tubulin. In host feeding treatment α-tubulin identified genes, while stress, combination α-Tubulin normalizing RT-qPCR data. Further, above-identified validated using based gene four objective namely, Myoinhibitory peptides (MIPs), Zinc_metalloprotease (Zn_Mp), fatty acid synthase (fas) alpha-glucosidase. Identified will facilitate studies future stress

Language: Английский

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