Intracellular signaling dynamics and their role in coordinating tissue repair

WIREs Systems Biology and Medicine, Год журнала: 2020, Номер 12(3)

Опубликована: Фев. 8, 2020

Abstract Tissue repair is a complex process that requires effective communication and coordination between cells across multiple tissues organ systems. Two of the initial intracellular signals encode injury initiate tissue responses are calcium extracellular signal‐regulated kinase (ERK). However, ERK signaling control variety cellular behaviors important for including motility, contractility, proliferation, as well activity several different transcription factors, making it challenging to relate specific their respective programs. This knowledge gap ultimately hinders development new wound healing therapies could take advantage native programs more effectively damage. The objective this review highlight roles dynamics mechanisms link during epithelial stromal repair. We detail how networks controlling can now also be dissected using classical signal processing techniques with advent biosensors optogenetic controllers. Finally, we advocate importance recognizing key links detection both organize execute coordinated response organs. article categorized under: Models Systems Properties Processes > Mechanistic Biological Mechanisms Cell Signaling Laboratory Methods Technologies Imaging Organ, Tissue, Physiological

Язык: Английский

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Calcium Imaging and the Curse of Negativity

Frontiers in Neural Circuits, Год журнала: 2021, Номер 14

Опубликована: Янв. 6, 2021

The imaging of neuronal activity using calcium indicators has become a staple modern neuroscience. However, without ground truths, there is real risk missing significant portion the responses. Here, we show that common assumption, non-negativity responses as detected by indicators, biases all levels frequently used analytical methods for these data. From extraction meaningful fluorescence changes to spike inference and analysis inferred spikes, each step risks because assumption non-negativity. We first negative deviations from baseline can exist in activity. Then, use simulated data test three popular algorithms image analysis, CaImAn, suite2p, CellSort, finding suite2p may be best suited large datasets. also tested included Cellsort, well dedicated MLspike CASCADE, found have limitations dealing with inhibited neurons. Among algorithms, FOOPSI, performed on neurons, but even this algorithm spurious spikes upon return signal baseline. As such, new approaches will needed before sensitively accurately further suggest avoiding that, assuming non-negativity, ignore Instead, exploratory step, k-means or PCA example, detect whether are present. Taking steps ensure inhibition, excitation,

Язык: Английский

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Mechanisms Underlying Influence of Bioelectricity in Development

Frontiers in Cell and Developmental Biology, Год журнала: 2022, Номер 10

Опубликована: Фев. 14, 2022

To execute the intricate process of development, cells coordinate across tissues and organs to determine where each cell divides differentiates. This coordination requires complex communication between cells. Growing evidence suggests that bioelectrical signals controlled via ion channels contribute during development. Ion collectively regulate transmembrane potential cells, their function plays a conserved role in development organisms from flies humans. Spontaneous calcium oscillations can be found nearly every type tissue, disruption these leads defects However, mechanism by which bioelectricity regulates is still unclear. play essential roles processes death, proliferation, migration, major canonical developmental signaling pathways. Previous reviews focus on for one affects morphogenesis, but there supports multiple different mechanisms are not mutually exclusive. Evidence contributing through mechanisms. Here, we review importance morphogenesis provide comprehensive several may act processes.

Язык: Английский

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Dietary amino acids promote glucagon-like hormone release to generate global calcium waves in adipose tissues in Drosophila

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 2, 2025

Propagation of intercellular calcium waves through tissues has been found to coordinate different multicellular responses. Nevertheless, our understanding how operate remains limited. In this study, we explore the real-time dynamics in Drosophila adipose tissues. We identify Adipokinetic Hormone (AKH), fly functional homolog glucagon, as key factor driving Ca2+ activities tissue. find that AKH, which is released into hemolymph from AKH-producing neurosecretory cells, stimulates larval fat by a previously unrecognized gap-junction-independent mechanism promote lipolysis. adult body, however, gap-junction-dependent are triggered presumably uniformly diffused AKH. Additionally, discover amino acids activate leading increased intracellular and AKH secretion. Altogether, show dietary regulate release cells brain, subsequently tissue, enhancing lipid metabolism. Intercellular (ICWs) responses, although precise mechanisms less understood. Here, authors nutrient-triggered hormone brain ICWs

Язык: Английский

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Calcium as a signal integrator in developing epithelial tissues

Physical Biology, Год журнала: 2018, Номер 15(5), С. 051001 - 051001

Опубликована: Апрель 3, 2018

Decoding how tissue properties emerge across multiple spatial and temporal scales from the integration of local signals is a grand challenge in quantitative biology. For example, collective behavior epithelial cells critical for shaping developing embryos. Understanding interpret diverse range to coordinate tissue-level processes requires systems-level understanding development. Integration signaling pathways that specify cell information second messengers such as calcium ions. Increasingly, specific roles have been uncovered throughout Calcium regulates many including division, migration, death, differentiation. However, pleiotropic ubiquitous nature implies additional functions remain be discovered. Here we review selection recent studies highlight important insights into are transduced by transients tissues. Quantitative imaging computational modeling provided occurs. Reverse-engineering conserved features signal mediated will enable novel approaches regenerative medicine synthetic control morphogenesis.

Язык: Английский

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Decoding Calcium Signaling Dynamics during Drosophila Wing Disc Development

Biophysical Journal, Год журнала: 2019, Номер 116(4), С. 725 - 740

Опубликована: Янв. 11, 2019

Язык: Английский

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Proteolytic activation of Growth-blocking peptides triggers calcium responses through the GPCR Mthl10 during epithelial wound detection

Developmental Cell, Год журнала: 2021, Номер 56(15), С. 2160 - 2175.e5

Опубликована: Июль 21, 2021

Язык: Английский

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Cell cycle controls long-range calcium signaling in the regenerating epidermis

The Journal of Cell Biology, Год журнала: 2023, Номер 222(7)

Опубликована: Апрель 27, 2023

Skin homeostasis is maintained by stem cells, which must communicate to balance their regenerative behaviors. Yet, how adult cells signal across tissue remains unknown due challenges in studying signaling dynamics live mice. We combined imaging the mouse basal cell layer with machine learning tools analyze patterns of Ca2+ signaling. show that display dynamic intercellular among local neighborhoods. find these signals are coordinated thousands and this coordination an emergent property layer. demonstrate G2 required initiate normal levels signaling, while connexin43 connects orchestrate tissue-wide Lastly, we drives cycle progression, revealing a communication feedback loop. This work provides resolution into at different stages coordinate during epidermal regeneration.

Язык: Английский

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Wound healing, calcium signaling, and other novel pathways are associated with the formation of butterfly eyespots

BMC Genomics, Год журнала: 2017, Номер 18(1)

Опубликована: Окт. 16, 2017

One hypothesis surrounding the origin of novel traits is that they originate from co-option pre-existing genes or larger gene regulatory networks into developmental contexts. Insights a trait's evolutionary origins can, thus, be gained via identification underlying trait development, and exploring whether those also function in other Here we investigate set associated with development eyespot color patterns, originated once within Nymphalid family butterflies. Although several have been identified, network remains largely unknown.In this study, next-generation sequencing transcriptome analyses were used to identify large Bicyclus anynana butterflies, at 3-6 h after pupation, prior differentiation rings. Eyespot-associated identified by comparing transcriptomes homologous micro-dissected wing tissues either develop do not eyespots wild-type mutant line Spotty, extra eyespots. Overall, 186 significantly up down-regulated compared not. Many differentially expressed yet annotated. New signaling pathways, including Toll, Fibroblast Growth Factor (FGF), extracellular signal-regulated kinase (ERK) and/or Jun N-terminal (JNK) pathways are for first time development. In addition, involved wound healing calcium found eyespots.Overall, study provides identity many new eyespots, suggests ancient may co-opted cells center pattern aid origins. transcription factors providing different identities distinct sectors, sexually dimorphic expression identified.

Язык: Английский

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Calcium-Binding Proteins with Disordered Structure and Their Role in Secretion, Storage, and Cellular Signaling

Biomolecules, Год журнала: 2018, Номер 8(2), С. 42 - 42

Опубликована: Июнь 19, 2018

Calcium is one of the most important second messengers and its intracellular signaling regulates many aspects cell physiology. ions, like phosphate are highly charged thus able to alter protein conformation upon binding; thereby they constitute key factors in signal transduction. One common calcium-binding structural motifs EF-hand, a well-defined helix-loop-helix domain, present proteins (CBPs). Nonetheless, some CBPs contain non-canonical, disordered motifs, which usually bind calcium with high capacity low affinity, represent subset specific functions, but these functions rarely involve signaling. When compared phosphorylation-mediated transduction, role intrinsic disorder significantly less prominent not direct. The list known examples intrinsically relatively short seems be linked secretion storage. Calcium-sensitive phosphatase calcineurin an exception, it represents example transient disorder, is, nevertheless, vital functioning this protein. underlying reason for different two main cellular systems appears gradient concentration, all living cells.

Язык: Английский

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