Frontiers in Cell and Developmental Biology,
Год журнала:
2020,
Номер
8
Опубликована: Окт. 6, 2020
Time-lapse
imaging
is
an
essential
tool
to
study
dynamic
biological
processes
that
cannot
be
discerned
from
fixed
samples
alone.
However,
cell-and
tissue-level
in
intact
animals
poses
numerous
challenges
if
the
organism
opaque
and/or
motile.
Explant
cultures
of
tissues
circumvent
some
these
challenges,
but
sample
drift
remains
a
considerable
obstacle.
We
employed
simple
yet
effective
technique
immobilize
medium-bathed
agarose.
applied
this
multiple
Drosophila
first-instar
larvae
adult
stages
various
orientations
and
with
no
evidence
anisotropic
pressure
or
stress
damage.
Using
method,
we
were
able
image
fine
features
for
up
18
hours
make
novel
observations.
Specifically,
report
fibers
characteristic
quiescent
neuroblasts
are
inherited
by
their
basal
daughters
during
reactivation;
lamina
developing
visual
system
assembled
roughly
2-3
columns
at
time;
glia
positions
development;
nuclear
envelopes
testis
cyst
stem
cells
do
not
break
down
completely
mitosis.
In
all,
demonstrate
our
protocol
wellsuited
tissue
immobilization
long-term
live
imaging,
enabling
new
insights
into
cell
dynamics
Drosophila.
WIREs Systems Biology and Medicine,
Год журнала:
2020,
Номер
12(3)
Опубликована: Фев. 8, 2020
Abstract
Tissue
repair
is
a
complex
process
that
requires
effective
communication
and
coordination
between
cells
across
multiple
tissues
organ
systems.
Two
of
the
initial
intracellular
signals
encode
injury
initiate
tissue
responses
are
calcium
extracellular
signal‐regulated
kinase
(ERK).
However,
ERK
signaling
control
variety
cellular
behaviors
important
for
including
motility,
contractility,
proliferation,
as
well
activity
several
different
transcription
factors,
making
it
challenging
to
relate
specific
their
respective
programs.
This
knowledge
gap
ultimately
hinders
development
new
wound
healing
therapies
could
take
advantage
native
programs
more
effectively
damage.
The
objective
this
review
highlight
roles
dynamics
mechanisms
link
during
epithelial
stromal
repair.
We
detail
how
networks
controlling
can
now
also
be
dissected
using
classical
signal
processing
techniques
with
advent
biosensors
optogenetic
controllers.
Finally,
we
advocate
importance
recognizing
key
links
detection
both
organize
execute
coordinated
response
organs.
article
categorized
under:
Models
Systems
Properties
Processes
>
Mechanistic
Biological
Mechanisms
Cell
Signaling
Laboratory
Methods
Technologies
Imaging
Organ,
Tissue,
Physiological
Frontiers in Neural Circuits,
Год журнала:
2021,
Номер
14
Опубликована: Янв. 6, 2021
The
imaging
of
neuronal
activity
using
calcium
indicators
has
become
a
staple
modern
neuroscience.
However,
without
ground
truths,
there
is
real
risk
missing
significant
portion
the
responses.
Here,
we
show
that
common
assumption,
non-negativity
responses
as
detected
by
indicators,
biases
all
levels
frequently
used
analytical
methods
for
these
data.
From
extraction
meaningful
fluorescence
changes
to
spike
inference
and
analysis
inferred
spikes,
each
step
risks
because
assumption
non-negativity.
We
first
negative
deviations
from
baseline
can
exist
in
activity.
Then,
use
simulated
data
test
three
popular
algorithms
image
analysis,
CaImAn,
suite2p,
CellSort,
finding
suite2p
may
be
best
suited
large
datasets.
also
tested
included
Cellsort,
well
dedicated
MLspike
CASCADE,
found
have
limitations
dealing
with
inhibited
neurons.
Among
algorithms,
FOOPSI,
performed
on
neurons,
but
even
this
algorithm
spurious
spikes
upon
return
signal
baseline.
As
such,
new
approaches
will
needed
before
sensitively
accurately
further
suggest
avoiding
that,
assuming
non-negativity,
ignore
Instead,
exploratory
step,
k-means
or
PCA
example,
detect
whether
are
present.
Taking
steps
ensure
inhibition,
excitation,
Frontiers in Cell and Developmental Biology,
Год журнала:
2022,
Номер
10
Опубликована: Фев. 14, 2022
To
execute
the
intricate
process
of
development,
cells
coordinate
across
tissues
and
organs
to
determine
where
each
cell
divides
differentiates.
This
coordination
requires
complex
communication
between
cells.
Growing
evidence
suggests
that
bioelectrical
signals
controlled
via
ion
channels
contribute
during
development.
Ion
collectively
regulate
transmembrane
potential
cells,
their
function
plays
a
conserved
role
in
development
organisms
from
flies
humans.
Spontaneous
calcium
oscillations
can
be
found
nearly
every
type
tissue,
disruption
these
leads
defects
However,
mechanism
by
which
bioelectricity
regulates
is
still
unclear.
play
essential
roles
processes
death,
proliferation,
migration,
major
canonical
developmental
signaling
pathways.
Previous
reviews
focus
on
for
one
affects
morphogenesis,
but
there
supports
multiple
different
mechanisms
are
not
mutually
exclusive.
Evidence
contributing
through
mechanisms.
Here,
we
review
importance
morphogenesis
provide
comprehensive
several
may
act
processes.
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 2, 2025
Propagation
of
intercellular
calcium
waves
through
tissues
has
been
found
to
coordinate
different
multicellular
responses.
Nevertheless,
our
understanding
how
operate
remains
limited.
In
this
study,
we
explore
the
real-time
dynamics
in
Drosophila
adipose
tissues.
We
identify
Adipokinetic
Hormone
(AKH),
fly
functional
homolog
glucagon,
as
key
factor
driving
Ca2+
activities
tissue.
find
that
AKH,
which
is
released
into
hemolymph
from
AKH-producing
neurosecretory
cells,
stimulates
larval
fat
by
a
previously
unrecognized
gap-junction-independent
mechanism
promote
lipolysis.
adult
body,
however,
gap-junction-dependent
are
triggered
presumably
uniformly
diffused
AKH.
Additionally,
discover
amino
acids
activate
leading
increased
intracellular
and
AKH
secretion.
Altogether,
show
dietary
regulate
release
cells
brain,
subsequently
tissue,
enhancing
lipid
metabolism.
Intercellular
(ICWs)
responses,
although
precise
mechanisms
less
understood.
Here,
authors
nutrient-triggered
hormone
brain
ICWs
Physical Biology,
Год журнала:
2018,
Номер
15(5), С. 051001 - 051001
Опубликована: Апрель 3, 2018
Decoding
how
tissue
properties
emerge
across
multiple
spatial
and
temporal
scales
from
the
integration
of
local
signals
is
a
grand
challenge
in
quantitative
biology.
For
example,
collective
behavior
epithelial
cells
critical
for
shaping
developing
embryos.
Understanding
interpret
diverse
range
to
coordinate
tissue-level
processes
requires
systems-level
understanding
development.
Integration
signaling
pathways
that
specify
cell
information
second
messengers
such
as
calcium
ions.
Increasingly,
specific
roles
have
been
uncovered
throughout
Calcium
regulates
many
including
division,
migration,
death,
differentiation.
However,
pleiotropic
ubiquitous
nature
implies
additional
functions
remain
be
discovered.
Here
we
review
selection
recent
studies
highlight
important
insights
into
are
transduced
by
transients
tissues.
Quantitative
imaging
computational
modeling
provided
occurs.
Reverse-engineering
conserved
features
signal
mediated
will
enable
novel
approaches
regenerative
medicine
synthetic
control
morphogenesis.
The Journal of Cell Biology,
Год журнала:
2023,
Номер
222(7)
Опубликована: Апрель 27, 2023
Skin
homeostasis
is
maintained
by
stem
cells,
which
must
communicate
to
balance
their
regenerative
behaviors.
Yet,
how
adult
cells
signal
across
tissue
remains
unknown
due
challenges
in
studying
signaling
dynamics
live
mice.
We
combined
imaging
the
mouse
basal
cell
layer
with
machine
learning
tools
analyze
patterns
of
Ca2+
signaling.
show
that
display
dynamic
intercellular
among
local
neighborhoods.
find
these
signals
are
coordinated
thousands
and
this
coordination
an
emergent
property
layer.
demonstrate
G2
required
initiate
normal
levels
signaling,
while
connexin43
connects
orchestrate
tissue-wide
Lastly,
we
drives
cycle
progression,
revealing
a
communication
feedback
loop.
This
work
provides
resolution
into
at
different
stages
coordinate
during
epidermal
regeneration.
One
hypothesis
surrounding
the
origin
of
novel
traits
is
that
they
originate
from
co-option
pre-existing
genes
or
larger
gene
regulatory
networks
into
developmental
contexts.
Insights
a
trait's
evolutionary
origins
can,
thus,
be
gained
via
identification
underlying
trait
development,
and
exploring
whether
those
also
function
in
other
Here
we
investigate
set
associated
with
development
eyespot
color
patterns,
originated
once
within
Nymphalid
family
butterflies.
Although
several
have
been
identified,
network
remains
largely
unknown.In
this
study,
next-generation
sequencing
transcriptome
analyses
were
used
to
identify
large
Bicyclus
anynana
butterflies,
at
3-6
h
after
pupation,
prior
differentiation
rings.
Eyespot-associated
identified
by
comparing
transcriptomes
homologous
micro-dissected
wing
tissues
either
develop
do
not
eyespots
wild-type
mutant
line
Spotty,
extra
eyespots.
Overall,
186
significantly
up
down-regulated
compared
not.
Many
differentially
expressed
yet
annotated.
New
signaling
pathways,
including
Toll,
Fibroblast
Growth
Factor
(FGF),
extracellular
signal-regulated
kinase
(ERK)
and/or
Jun
N-terminal
(JNK)
pathways
are
for
first
time
development.
In
addition,
involved
wound
healing
calcium
found
eyespots.Overall,
study
provides
identity
many
new
eyespots,
suggests
ancient
may
co-opted
cells
center
pattern
aid
origins.
transcription
factors
providing
different
identities
distinct
sectors,
sexually
dimorphic
expression
identified.
Biomolecules,
Год журнала:
2018,
Номер
8(2), С. 42 - 42
Опубликована: Июнь 19, 2018
Calcium
is
one
of
the
most
important
second
messengers
and
its
intracellular
signaling
regulates
many
aspects
cell
physiology.
ions,
like
phosphate
are
highly
charged
thus
able
to
alter
protein
conformation
upon
binding;
thereby
they
constitute
key
factors
in
signal
transduction.
One
common
calcium-binding
structural
motifs
EF-hand,
a
well-defined
helix-loop-helix
domain,
present
proteins
(CBPs).
Nonetheless,
some
CBPs
contain
non-canonical,
disordered
motifs,
which
usually
bind
calcium
with
high
capacity
low
affinity,
represent
subset
specific
functions,
but
these
functions
rarely
involve
signaling.
When
compared
phosphorylation-mediated
transduction,
role
intrinsic
disorder
significantly
less
prominent
not
direct.
The
list
known
examples
intrinsically
relatively
short
seems
be
linked
secretion
storage.
Calcium-sensitive
phosphatase
calcineurin
an
exception,
it
represents
example
transient
disorder,
is,
nevertheless,
vital
functioning
this
protein.
underlying
reason
for
different
two
main
cellular
systems
appears
gradient
concentration,
all
living
cells.