Mass spectrometry-based metabolomics: a guide for annotation, quantification and best reporting practices

Nature Methods, Год журнала: 2021, Номер 18(7), С. 747 - 756

Опубликована: Июль 1, 2021

Язык: Английский

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Soil biota, antimicrobial resistance and planetary health

Environment International, Год журнала: 2019, Номер 131, С. 105059 - 105059

Опубликована: Июль 30, 2019

The concept of planetary health acknowledges the links between ecosystems, biodiversity and human well-being. Soil, critical component interconnected ecosystem, is most biodiverse habitat on Earth, soil microbiomes play a major role in well-being through ecosystem services such as nutrient cycling, pollutant remediation synthesis bioactive compounds antimicrobials. Soil also natural source antimicrobial resistance, which often termed intrinsic resistance. However, increasing use misuse antimicrobials humans animals recent decades has increased both diversity prevalence resistance soils, particularly areas affected by animal wastes, organic manures reclaimed wastewater, air transmission. Antimicrobials are two sides sword, while essential care; globally, jeopardizing effectiveness drugs, thus threatening health. crucial pathway exposed to determinants, including those harbored pathogens. In this review, we nexus focus discuss illustrate impacts This review examines sources dynamics soils uses perspective track movement antimicrobial-resistance genes environmental compartments, soil, water, food air.

Язык: Английский

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Fungal Endophytes as Efficient Sources of Plant-Derived Bioactive Compounds and Their Prospective Applications in Natural Product Drug Discovery: Insights, Avenues, and Challenges

Microorganisms, Год журнала: 2021, Номер 9(1), С. 197 - 197

Опубликована: Янв. 19, 2021

Fungal endophytes are well-established sources of biologically active natural compounds with many producing pharmacologically valuable specific plant-derived products. This review details typical medicinal several classes, including alkaloids, coumarins, flavonoids, glycosides, lignans, phenylpropanoids, quinones, saponins, terpenoids, and xanthones that produced by endophytic fungi. covers the studies carried out since first report taxol biosynthesis Taxomyces andreanae in 1993 up to mid-2020. The article also highlights prospects endophyte-dependent such bottlenecks commercialization this novel approach area drug discovery. After recent updates field 'omics' 'one strain compounds' (OSMAC) approach, fungal have emerged as strong unconventional source prized

Язык: Английский

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A community resource for paired genomic and metabolomic data mining

Nature Chemical Biology, Год журнала: 2021, Номер 17(4), С. 363 - 368

Опубликована: Фев. 15, 2021

Genomics and metabolomics are widely used to explore specialized metabolite diversity. The Paired Omics Data Platform is a community initiative systematically document links between metabolome (meta)genome data, aiding identification of natural product biosynthetic origins structures.

Язык: Английский

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Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability

Materials Today Bio, Год журнала: 2023, Номер 19, С. 100560 - 100560

Опубликована: Янв. 21, 2023

Filamentous fungi drive carbon and nutrient cycling across our global ecosystems, through its interactions with growing decaying flora their constituent microbiomes. The remarkable metabolic diversity, secretion ability, fiber-like mycelial structure that have evolved in filamentous been increasingly exploited commercial operations. industrial potential of fermentation ranges from the discovery bioproduction enzymes bioactive compounds, decarbonization food material production, to environmental remediation enhanced agricultural production. Despite fundamental impact ecology biotechnology, molds mushrooms not, to-date, significantly intersected synthetic biology ways comparable other cell factories (e.g. Escherichia coli,Saccharomyces cerevisiae, Komagataella phaffii). In this review, we summarize a suite computational tools for mining, engineering optimization as chassis. A combination methods genetic engineering, mutagenesis, experimental evolution, modeling can be used address strain development bottlenecks established emerging industries. These include slow mycelium growth rate, low production yields, non-optimal alternative feedstocks, difficulties downstream purification. scope biomanufacturing, then detail previous efforts improving key by targeting protein processing pathways, hyphae morphogenesis, transcriptional control. Bringing practices into hidden world will serve expand limited panel host organisms allow commercially-feasible environmentally-sustainable enzymes, chemicals, therapeutics, foods, materials future.

Язык: Английский

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Current trends, limitations and future research in the fungi?

Fungal Diversity, Год журнала: 2024, Номер 125(1), С. 1 - 71

Опубликована: Март 20, 2024

Abstract The field of mycology has grown from an underappreciated subset botany, to a valuable, modern scientific discipline. As this study grown, there have been significant contributions science, technology, and industry, highlighting the value fungi in era. This paper looks at current research, along with existing limitations, suggests future areas where scientists can focus their efforts, mycology. We show how become important emerging diseases medical discuss trends potential drug novel compound discovery. explore phylogenomics, its potential, outcomes address question phylogenomics be applied fungal ecology. In addition, functional genomics studies are discussed importance unravelling intricate mechanisms underlying behaviour, interactions, adaptations, paving way for comprehensive understanding biology. look research building materials, they used as carbon sinks, biocircular economies. numbers always great interest often written about estimates varied greatly. Thus, we needs order obtain more reliable estimates. aspects machine learning (AI) it mycological research. Plant pathogens affecting food production systems on global scale, such, needed area, particularly disease detection. latest data High Throughput Sequencing if still gaining new knowledge same rate before. A review nanotechnology is provided addressed. Arbuscular Mycorrhizal Fungi addressed acknowledged. Fungal databases becoming important, therefore provide major databases. Edible medicinal huge medicines, especially Asia prospects discussed. Lifestyle changes (e.g., endophytes, pathogens, and/or saprobes) also extremely trend special issue Diversity.

Язык: Английский

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Fungal Metabolomics: A Comprehensive Approach to Understanding Pathogenesis in Humans and Identifying Potential Therapeutics

Journal of Fungi, Год журнала: 2025, Номер 11(2), С. 93 - 93

Опубликована: Янв. 24, 2025

Metabolomics has emerged as a transformative tool in the study of microbes, including pathogenic fungi, facilitating identification unique metabolic profiles that elucidate their mechanisms, host interactions, and treatment resistance. This review highlights key applications metabolomics understanding fungal metabolites essential for human virulence, such mycotoxins produced by various species, Aspergillus fumigatus (gliotoxin, fumagillins) Candida species (phenylethyl alcohol, TCA cycle metabolites), secondary contribute to pathogenicity. It also explores adaptations fungi relation drug resistance biofilm formation, revealing alterations pathways during infection, seen C. albicans auris. Furthermore, aids deciphering host–pathogen showcasing how like Cryptococcus neoformans modify metabolism promote survival evade immune responses. The antifungal mechanisms benefited from metabolomic approaches, identifying specific metabolite patterns signify resistance, Candidozyma (Candida) auris, informing new therapeutic strategies. integration with other omics technologies is paving way comprehensive biology pathogenesis. Such multi-omics approaches are crucial discovering targets developing innovative treatments. Thus, purpose this provide an overview revolutionizing our pathogenesis, highlight its potential improving

Язык: Английский

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Inhibition of glycolysis alleviates lipopolysaccharide‐induced acute lung injury in a mouse model

Journal of Cellular Physiology, Год журнала: 2018, Номер 234(4), С. 4641 - 4654

Опубликована: Сен. 7, 2018

Abstract Gluconic metabolic reprogramming, immune response, and inflammation are intimately linked. Glycolysis involves in the pathologic progress acute chronic inflammatory diseases. However, involvement of glycolysis lung injury (ALI) is still unclear. This study investigated role an animal model ALI. First, we found that lactate content serum was remarkably increased ALI patients a murine induced by intratracheal administration lipopolysaccharide (LPS). The key proteins involving were robustly elevated, including HK2 , PKM2 HIF‐1α . Intriguingly, inhibition 2‐deoxyglucose (2‐DG) pronouncedly attenuated tissue pathological injury, accumulation neutrophil, oxidative stress, expression proinflammatory factors mice LPS. 2‐DG treatment also strongly suppressed activation NOD‐like receptor (NLR) family pyrin domain‐containing protein 3 (NLRP3) inflammasome. Furthermore, response primary macrophages activated LPS vitro. We reduced LPS, tumor necrosis factor‐α messenger RNA (mRNA), pro‐interleukin (IL)‐1β mRNA, pro‐IL‐18 NLRP3 caspase‐1 IL‐1β protein. Altogether, these data provide novel link between gluconic metabolism reprogramming uncontrolled suggests glycolytic as effective anti‐inflammatory strategy treating

Язык: Английский

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Harnessing diverse transcriptional regulators for natural product discovery in fungi

Natural Product Reports, Год журнала: 2019, Номер 37(1), С. 6 - 16

Опубликована: Апрель 29, 2019

Covering: Up to March 2019 Secondary metabolites (SMs) are chemical entities produced by organisms in response environmental stimuli and as a defense against biological warfare. The production of SMs is controlled hierarchical regulatory network involving core factors that orchestrate transcriptional activation SM gene clusters. In the past few years, significant achievements have been made discovery novel fungal natural products genetic manipulations various types regulators. this review, we summarized representative regulators for secondary metabolism focused on strategies exploitation these their application finding structures.

Язык: Английский

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Metabolomics and genomics in natural products research: complementary tools for targeting new chemical entities

Natural Product Reports, Год журнала: 2021, Номер 38(11), С. 2041 - 2065

Опубликована: Янв. 1, 2021

Here we provide a comprehensive guide for studying natural product biosynthesis using genomics, metabolomics, and their integrated datasets. We emphasize strategies critical outlook on remaining challenges in the field.

Язык: Английский

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