Опубликована: Янв. 1, 2024
Язык: Английский
Frontiers in Bioscience-Scholar, Год журнала: 2022, Номер 14(3), С. 22 - 22
Опубликована: Авг. 1, 2022
Alzheimer's disease (AD) is the most common neurodegenerative disorder, resulting in memory loss, cognitive decline, bodily function impairment, and finally death. The growing number of people suffering from AD increasingly urges development effective early diagnosis monitoring techniques. Here, we review recent developments field Raman-based techniques, which have shown a significant potential identifying by detecting specific biomarkers biological fluids, as well providing fundamental insights into key molecules involved progression or analysis histological specimens patients with AD. These techniques comprise spontaneous resonant Raman spectroscopies, exploit plasmon- fiber- enhanced effects, such surface-, tip- involve non-linear like coherent scattering. scientific efforts employed up to now rapid technological advancements optical detection instruments (spectrometers, lasers, substrates for analysis, etc.) diffusion advanced data processing methods suggest leading role perspective preclinical clinical
Язык: Английский
Процитировано
17
Biosensors, Год журнала: 2023, Номер 13(5), С. 499 - 499
Опубликована: Апрель 25, 2023
Surface-enhanced Raman spectroscopy (SERS) applications in clinical diagnosis and spectral pathology are increasing due to the potential of technique bio-barcode incipient differential diseases via real-time monitoring biomarkers fluids biomolecular fingerprinting. Additionally, rapid advancements micro/nanotechnology have a visible influence all aspects science life. The miniaturization enhanced properties materials at micro/nanoscale transcended confines laboratory revolutionizing domains such as electronics, optics, medicine, environmental science. societal technological impact SERS biosensing by using semiconductor-based nanostructured smart substrates will be huge once minor technical pitfalls solved. Herein, challenges routine testing addressed order understand context how can perform real, vivo sampling bioassays for early neurodegenerative disease (ND) diagnosis. main interest translating into practice is reinforced practical advantages: portability designed setups, versatility nanomaterials various matter costs, readiness, reliability. As we present this review, frame technology readiness levels (TRL), current maturity reached biosensors, particular that zinc oxide (ZnO)-based hybrid substrates, situated development level TRL 6 (out 9 levels). Three-dimensional, multilayered provide additional plasmonic hot spots z-axis key importance designing highly performant biosensors detection ND biomarkers.
Язык: Английский
Процитировано
10
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
ACS Applied Materials & Interfaces, Год журнала: 2020, Номер 12(28), С. 31137 - 31147
Опубликована: Июнь 18, 2020
Core–shell particles made of calcium carbonate and coated with biocompatible polymers using the Layer-by-Layer technique can be considered as a unique drug-delivery platform that enables us to load different therapeutic compounds, exhibits high biocompatibility, integrate several stimuli-responsive mechanisms for drug release. However, before implementation diagnostic or purposes, such core–shell require comprehensive in vivo evaluation terms physicochemical pharmacokinetic properties. Positron emission tomography (PET) is an advanced imaging biodistribution carriers; nevertheless, incorporation positron emitters these carriers needed. Here, first time, we demonstrate radiolabeling approaches sizes (CaCO3 micron-sized (MicCSPs) CaCO3 submicron-sized (SubCSPs)) precisely determine their after intravenous administration rats. For this, methods have been developed, where emitter (68Ga) was incorporated into particle's core (co-precipitation approach) onto surface shell (either layer coating adsorption approaches). According obtained data, radiochemical bounding stability 68Ga strongly depend on used approach, co-precipitation method has shown best human serum (96–98.5% both types particles). Finally, size-dependent effect particles' distribution specific organ uptake, combination techniques, PET, computerized (CT), well radiometry separate organs. Thus, our findings open up new perspectives CaCO3-radiolabeled further clinical practice.
Язык: Английский
Процитировано
25
The Analyst, Год журнала: 2023, Номер 148(8), С. 1848 - 1857
Опубликована: Янв. 1, 2023
Molecular SERS analysis of 5- S -cysteinyl-dopamine and its detection in complex artificial fluids as a novel biomarker Parkinson's disease.
Язык: Английский
Процитировано
8
Nanoscale, Год журнала: 2024, Номер 16(25), С. 11879 - 11913
Опубликована: Янв. 1, 2024
Brain disorders, including neurodegenerative diseases (NDs) and traumatic brain injury (TBI), present significant challenges in early diagnosis intervention. Conventional imaging modalities, while valuable, lack the molecular specificity necessary for precise disease characterization. Compared to study of conventional tissues, liquid biopsy, which focuses on blood, tear, saliva, cerebrospinal fluid (CSF), also unveils a myriad underlying processes, providing abundant predictive clinical information. In addition, biopsy is minimally- non-invasive, highly repeatable, offering potential continuous monitoring. Raman spectroscopy (RS), with its ability provide rich information cost-effectiveness, holds great transformative advancements detection understanding biochemical changes associated NDs TBI. Recent developments enhancement technologies advanced data analysis methods have enhanced applicability RS probing intricate signatures within biological fluids, new insights into pathology. This review explores growing role as promising emerging tool particularly through biopsy. It discusses current landscape future prospects highlighting non-invasive molecularly specific diagnostic tool.
Язык: Английский
Процитировано
2
ACS Sensors, Год журнала: 2024, Номер 9(7), С. 3496 - 3501
Опубликована: Июнь 24, 2024
Here, we describe a SERS-based vertical flow assay as platform technology suitable for point-of-care (POC) diagnostic testing. A capture substrate is constructed from filter paper embedded with spherical gold nanoparticles (AuNPs) and functionalized an appropriate antibody. The loaded into filtration device connected to syringe rapidly repeatedly pass the sample through sensor efficient antigen binding. then labeled SERS-active detection probe. We show that only few Raman reporter molecules, exclusively located adjacent plasmonic substrate, generate detectible signals. To maximize signal underutilized employ secondary enhancing probe undergoes antibody-directed assembly form core–satellites. This facile enhancement step provides 3.5-fold increase in limit of 0.23 ng/mL (1.6 pM) human IgG. work highlights potential rationally design architectures using widely available reproducible AuNPs achieve large SERS enhancements highly sensitive POC diagnostics.
Язык: Английский
Процитировано
2
IEEE Reviews in Biomedical Engineering, Год журнала: 2022, Номер 16, С. 530 - 559
Опубликована: Март 23, 2022
The study of ocular manifestations neurodegenerative disorders, Oculomics, is a growing field investigation for early diagnostics, enabling structural and chemical biomarkers to be monitored overtime predict prognosis. Traumatic brain injury (TBI) triggers cascade events harmful the brain, which can lead neurodegeneration. TBI, termed "silent epidemic" becoming leading cause death disability worldwide. There currently no effective diagnostic tool yet, early-intervention known considerably shorten hospital stays, improve outcomes, fasten neurological recovery lower mortality rates, highlighting unmet need techniques capable rapid accurate point-of-care implemented in earliest stages. This review focuses on latest advances main neuropathophysiological responses achievements shortfalls TBI methods. Validated emerging TBI-indicative are outlined linked neuro-disorders. Methods detecting categorised along with prospective physical sensing techniques. Particular attention drawn potential Raman spectroscopy as non-invasive molecular signatures projections laying platform first tangible path towards alternative technologies TBI.
Язык: Английский
Процитировано
10
DOAJ (DOAJ: Directory of Open Access Journals), Год журнала: 2022, Номер 3
Опубликована: Янв. 1, 2022
In recent years, Raman spectroscopy has been more and frequently applied to address research questions in neuroscience. As a non-destructive technique based on inelastic scattering of photons, it can be used for wide spectrum applications including neurooncological tumor diagnostics or analysis misfolded protein aggregates involved neurodegenerative diseases. Progress the technical development this method allows an increasingly detailed biological samples may therefore open new fields applications. The goal our review is provide introduction into scattering, its practical usage also commonly associated pitfalls. Furthermore, intraoperative assessment recurrence using histology images as well search non-invasive ways diagnosis diseases are discussed. Some mentioned here serve basis possibly set course future use clinical practice. Covering broad range content, overview not only quick accessible reference tool but in-depth information specific subtopic interest.
Язык: Английский
Процитировано
9
Journal of The Electrochemical Society, Год журнала: 2023, Номер 170(1), С. 017503 - 017503
Опубликована: Янв. 1, 2023
In this report, a novel electrochemical and colorimetric dual-mode sensing system was developed for the sensitive selective detection of DA. The large surface area CoOOH excellent conductivity CB endow with high sensitivity. oxidase-like activity will convert colorless TMB into blue oxidation product OX TMB, appearing an absorption peak at 652 nm correspondingly. And addition DA inhibit oxidase followed by reducing in absorption. Our device provided wider range from 1 to 30 μ M 950 through methods, respectively. Furthermore, biosensor good biocompatibility has been successfully used in-vitro tracking efflux live PC12 cell after being stimulated, which important scientific practical value clinical diagnosis monitoring.
Язык: Английский
Процитировано
5