DNA Walkers for Biosensing Development

Advanced Science, Год журнала: 2022, Номер 9(18)

Опубликована: Апрель 22, 2022

Abstract The ability to design nanostructures with arbitrary shapes and controllable motions has made DNA nanomaterials used widely construct diverse nanomachines various structures functions. exhibit excellent properties, including programmability, stability, biocompatibility, can be modified different functional groups. Among these nanoscale architectures, walker is one of the most popular nanodevices ingenious flexible function. In past several years, walkers have amazing progress ranging from structural biological applications constructing biosensors for detection cancer‐associated biomarkers. this review, key driving forces are first summarized. Then, numbers legs introduced. Furthermore, biosensing detection‐ nucleic acids, proteins, ions, bacteria Finally, new frontiers opportunities developing walker‐based discussed.

Язык: Английский

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A Smart Deoxyribozyme‐Programmable Catalytic DNA Circuit for High‐Contrast MicroRNA Imaging

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(33)

Опубликована: Июнь 28, 2023

Synthetic catalytic DNA circuits have been recognized as a promising signal amplification toolbox for sensitive intracellular imaging, yet their selectivity and efficiency are always constrained by uncontrolled off-site leakage inefficient on-site circuitry activation. Thus, the endogenously controllable exposure/activation of is highly desirable achieving selective imaging live cells. Herein, an activated DNAzyme strategy was facilely integrated with circuit guiding efficient microRNA in vivo. To prevent activation, constitute initially caged without sensing functions, which could be selectively liberated amplifier to guarantee high-contrast target This intelligent modulation can tremendously expand these molecularly engineered biological systems.

Язык: Английский

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DNA as a universal chemical substrate for computing and data storage

Nature Reviews Chemistry, Год журнала: 2024, Номер 8(3), С. 179 - 194

Опубликована: Фев. 9, 2024

Язык: Английский

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Framework Nucleic Acid-Based Spatial-Confinement Amplifier for miRNA Imaging in Living Cells

Analytical Chemistry, Год журнала: 2022, Номер 94(6), С. 2934 - 2941

Опубликована: Фев. 2, 2022

Real-time in situ monitoring of miRNAs living cells is often appealed to signal amplifiers tackle their low abundance challenges. However, the poor kinetics and potential interferences from complex intracellular environment hamper its widespread applications vivo. Herein, we report a framework nucleic acid (FNA)-based nonenzymatic spatial-confinement amplifier for rapid reliable miRNA imaging. The consists localized catalytic hairpin assembly (L-CHA) reactor encapsulated inner cavity an FNA (a 20 bp cube). L-CHA certainly confined internal frame by integrating two probes (H1 H2) within DNA strand harnessing it opposite angles cube. We find that stability remarkably improved due protection FNA. More importantly, effect can endow with enhanced local concentrations reagents (5000-fold), thereby accelerating reaction rate improving dynamic performance (up 14.34-fold). With these advantages, proposed enable accurate effective expression levels poses great medical diagnostics biomedical research.

Язык: Английский

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Interplay between LncRNAs and microRNAs in Breast Cancer

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(9), С. 8095 - 8095

Опубликована: Апрель 30, 2023

(1) Although long noncoding RNAs (lncRNAs) are known to be precursors of microRNAs (miRNAs), they frequently act as competing endogoneous (ceRNAs), yet still their interplay with miRNA is not well known. However, interaction miRNAs may result in the modulation action. (2) To determine contribution these RNA molecules tumor resistance chemotherapeutic drugs, it essential consider only oncogenic and suppressive function but also impact lncRNAs on miRNAs. Therefore, we performed an extensive search different databases including PubMed. (3) The present study concerns between regulatory post-transcriptional network drugs used treatment breast cancer. (4) Consideration this improve for new circumvent chemoresistance.

Язык: Английский

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Advancing Aggregation‐Induced Emission‐Derived Biomaterials in Viral, Tuberculosis, and Fungal Infectious Diseases

Aggregate, Год журнала: 2024, Номер unknown

Опубликована: Дек. 2, 2024

ABSTRACT Contagious diseases caused by different types of highly contagious pathogens, such as SARS‐CoV‐2, monkeypox virus, Mycobacterium tuberculosis , and human immunodeficiency could trigger global outbreaks bring a huge public health burden. Advanced diagnostic, therapeutic, preventive strategies are urgently needed to deal with the epidemic diseases. Aggregation‐induced emission (AIE) has emerged one promising candidates that exhibit tunable photophysical properties, high biocompatibility, exceptional photostability, distinguishing aggregation‐enhanced fluorescence. As result, they offer effective for diagnosis, treatment, prevention This review systematically outlined latest research progress AIE‐based biomaterials mechanisms in The versatility AIE molecules, well efficient fluorescence potential innovative combat these challenges. Thanks recent advances materials science better understanding aggregation‐induced luminogens (AIEgens), AIEgens have great provide solutions detection, By reviewing state‐of‐the‐art methods killing, agents highlighting technological developments, this outlook aims promote development new means control emerging, re‐emerging, major further activities critical area research.

Язык: Английский

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Advances in DNA walking nanomachine‐based biosensors

Deleted Journal, Год журнала: 2024, Номер 2(1)

Опубликована: Янв. 1, 2024

Abstract The field of DNA nanotechnology has evolved beyond the realm controllable movements and randomly shaped nanostructures, now encompassing a diverse array nanomachines, each with unique nanostructures biofunctional attributes. These boast exceptional characteristics such as programmability, integrability, biocompatibility, universality. Among this variety, walking nanomachines have emerged one most prominent nanomotors, distinguished by their ingenious design comprehensive functionality. In recent times, these walkers witnessed remarkable advancements in areas ranging from nanostructural designs to biological applications, including creation sophisticated biosensors capable efficiently detecting tumor‐related biomarkers bioactive substances. This review delves into operational mechanisms which are driven processes protease DNAzyme action well strand displacement photoactivated reactions. It further provides overview different dimensional (1D, 2D, 3D) tracks. A subsequent section introduces biosensing applications electrochemical, optical, other biosensors. concludes forward‐looking perspective on novel challenges developing nanomachine‐based

Язык: Английский

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Self‐Assembled DNA Nanospheres Driven by Carbon Dots for MicroRNAs Imaging in Tumor via Logic Circuit

Small, Год журнала: 2024, Номер 20(25)

Опубликована: Янв. 17, 2024

Abstract DNA nanostructures with diverse biological functions have made significant advancements in biomedical applications. However, a universal strategy for the efficient production of is still lacking. In this work, facile and mild method presented self‐assembling polyethylenimine‐modified carbon dots (PEI‐CDs) into nanospheres called CANs at room temperature. This makes universally applicable to multiple applications involving various types DNA. Due ultra‐small size strong cationic charge PEI‐CDs, exhibit dense structure high loading capacity encapsulated while providing excellent stability by protecting from enzymatic hydrolysis. Additionally, Mg 2+ incorporated form Mg@CANs which enriches performance enables subsequent imaging exogenous . Especially, DNAzyme logic gate system that contains AND OR constructed successfully delivered tumor cells vitro vivo. They can be specifically activated endogenic human apurinic/apyrimidinic endonuclease 1 recognize expression levels miRNA‐21 miRNA‐155 sites biocomputing. A versatile pattern delivery flexible circuits miRNAs are developed.

Язык: Английский

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Emerging Trends in DNA Nanotechnology-Enabled Cell Surface Engineering

JACS Au, Год журнала: 2025, Номер 5(2), С. 550 - 570

Опубликована: Фев. 6, 2025

Cell surface engineering is a rapidly advancing field, pivotal for understanding cellular physiology and driving innovations in biomedical applications. In this regard, DNA nanotechnology offers unprecedented potential precisely manipulating functionalizing cell surfaces by virtue of its inherent programmability versatile functionalities. Herein, Perspective provides comprehensive overview emerging trends engineering, focusing on key nanostructure-based tools, their roles regulating physiological processes, We first discuss the strategies integrating molecules onto surfaces, including attachment oligonucleotides higher-order nanostructure. Second, we summarize impact DNA-based various such as membrane protein degradation, signaling transduction, intercellular communication, construction artificial components. Third, highlight applications DNA-engineered targeted therapies cancer inflammation, well capture/protection diagnostic detection. Finally, address challenges future directions nanotechnology-based engineering. This aims to provide valuable insights rational design contributing development precise personalized medicine.

Язык: Английский

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Mitochondria-Targeted DNA-Based Nanoprobe for In Situ Monitoring of the Activity of the mtDNA Repair Enzyme and Evaluating Tumor Radiosensitivity

Analytical Chemistry, Год журнала: 2025, Номер 97(1), С. 382 - 391

Опубликована: Янв. 2, 2025

Evaluating tumor radiosensitivity is beneficial for the prediction of treatment efficacy, customization plans, and minimization side effects. Tracking mitochondrial DNA (mtDNA) repair process helps to assess as mtDNA determines fate cell under radiation-induced damage. However, current probes developed monitor levels enzymes suffered from complex synthesis, uncontrollable preparation, limited selectivity, poor organelle-targeting ability. Especially, correlation between activity inherent tumors has not yet been explored. Here, we present a mitochondria-targeted DNA-based nanoprobe (TPP-Apt-tFNA) in situ monitoring enzyme evaluating radiosensitivity. TPP-Apt-tFNA consists tetrahedral framework precisely modified with three functional modules on each vertexes, that is, cell-targeting aptamer, mitochondrion-targeting moiety, apurinic/apyrimidinic endonuclease 1 (APE1)-responsive molecule beacon. Once selectively internalized by cells, targeted mitochondrion specifically recognized APE1 activate fluorescence, allowing observation activity. The showed elevated mitochondria cells oxidative stress. Moreover, enabled illumination different APE1-mediated cycle phases. Furthermore, using vitro vivo, found high repair, which allowed them recover lesions, had low sensitivity radiation an unsatisfactory radiotherapy outcome. Our work provides new imaging tool exploring roles diverse biological processes guiding treatment.

Язык: Английский

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