Biosensors and Bioelectronics, Год журнала: 2022, Номер 204, С. 114078 - 114078
Опубликована: Фев. 12, 2022
Язык: Английский
Analytical Chemistry, Год журнала: 2023, Номер 95(18), С. 7372 - 7378
Опубликована: Апрель 25, 2023
Electrochemiluminescence (ECL) microscopy is an emerging technique with new applications such as imaging of single entities and cells. Herein, we have developed a bimodal bicolor approach to record both positive ECL (PECL: light-emitting object on dark background) shadow label-free (SECL: nonemissive shadowing the background luminescence) images This result simultaneous emissions [Ru(bpy)3]2+ used label cellular membrane (PECL) [Ir(sppy)3]3– dissolved in solution (SECL). By spectrally resolving emission wavelengths, recorded same cells PECL SECL modes using (λmax = 620 nm) 515 luminescence, respectively. shows distribution labels attached membrane, whereas reflects local diffusional hindrance reagents by each cell. The high sensitivity surface-confined features reported are demonstrated cell–cell contacts during mitosis process. Furthermore, comparison demonstrates differential diffusion tri-n-propylamine through permeabilized cell membranes. Consequently, this dual enables morphology adhering surface can significantly contribute multimodal bioassays different luminescent systems.
Язык: Английский
Процитировано
27
Analytical Chemistry, Год журнала: 2023, Номер 95(1), С. 374 - 387
Опубликована: Янв. 10, 2023
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTElectrochemiluminescence from Single Molecule to ImagingJinrun DongJinrun DongLaboratory of Experimental Physical Biology, Department Chemistry, Zhejiang University, Hangzhou, 310027, ChinaMore by Jinrun DongView Biography and Jiandong Feng*Jiandong FengLaboratory China*Email: [email protected]More FengView Biographyhttps://orcid.org/0000-0002-2143-3986Cite this: Anal. Chem. 2023, 95, 1, 374–387Publication Date (Web):January 10, 2023Publication History Received22 October 2022Published online10 January 2023Published inissue 10 2023https://pubs.acs.org/doi/10.1021/acs.analchem.2c04663https://doi.org/10.1021/acs.analchem.2c04663review-articleACS PublicationsCopyright © 2023 American Chemical SocietyRequest reuse permissionsArticle Views2204Altmetric-Citations2LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence for given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Electrodes,Fluorescence imaging,Imaging,Microscopy,Molecules Get e-Alerts
Язык: Английский
Процитировано
25
Analytical Chemistry, Год журнала: 2024, Номер 96(15), С. 5852 - 5859
Опубликована: Апрель 1, 2024
A multicolor electrochemiluminescence (ECL) biosensor based on a closed bipolar electrode (BPE) array was proposed for the rapid and intuitive analysis of three prostate cancer staging indicators. First, [Irpic-OMe], [Ir(ppy)2(acac)], [Ru(bpy)3]2+ were applied as blue, green, red ECL emitters, respectively, whose mixed emission colors covered whole visible region by varying voltages. Afterward, we designed simple Mg2+-dependent DNAzyme (MNAzyme)-driven tripedal DNA walker (TD walker) to release output DNAs. Immediately after, DNAs added cathodic reservoirs BPE incubation. After that, found that from anode changed driving voltage 8.0 V applied, mainly due changes in interfacial potential faradaic currents at two poles BPE. Via optimization experimental parameters, cutoff values such indicators different clinical stages could be identified instantly with naked eye, standard precision swatches multiple prepared. Finally, order precisely determine stage, device used analysis, resulting images then compared swatches, laying way accurate therapy.
Язык: Английский
Процитировано
11
Analytical Chemistry, Год журнала: 2024, Номер 96(4), С. 1651 - 1658
Опубликована: Янв. 19, 2024
The development of a highly accurate electrochemiluminescence (ECL) signal switch to avoid nonspecific stimulus responses is currently significant and challenging task. Here, we constructed universal utilizing luminophore–quencher pair mesostructured silica xerogel-confined polymer gold nanoparticles (Au NPs) that can accurately detect low-abundance epigenetic markers in complex sample systems. Notably, the ECL encapsulated xerogel acts as luminophore, which demonstrated specific dependence on Au NPs-mediated energy transfer quenching. To demonstrate feasibility, specifically labeled 5-hydroxymethylcytosine (5hmC) site random sequence using double-stranded (dsDNA) tag was skillfully designed with CRISPR/Cas12a activator recombinant polymerase amplification (RPA) template. After by RPA, large amount dsDNA generated initiate trans-cleavage activity subsequently activate switch, allowing for precise quantification 5hmC. improves stability luminophore prevents responses, providing new paradigm constructing high-precision biosensors.
Язык: Английский
Процитировано
10
Nano Letters, Год журнала: 2024, Номер 24(15), С. 4665 - 4671
Опубликована: Апрель 8, 2024
Effective bimetallic nanoelectrocatalysis demands precise control of composition, structure, and understanding catalytic mechanisms. To address these challenges, we employ a two-in-one approach, integrating online synthesis with real-time imaging Au@Metal core–shell nanoparticles (Au@M NPs) via electrochemiluminescence microscopy (ECLM). Within 120 s, electrodeposition in situ activity screening alternate. ECLM captures transient faradaic processes during potential switches, visualizes electrochemical real-time, tracks dynamics at the single-particle level. Analysis using ECL photon flux density eliminates size effects yields quantitative electrocatalytic results. Notably, nonlinear trend corresponding to shell metal Au surface atomic ratio is discerned, quantifying optimal component Au@M NPs. This approach offers comprehensive behavior deposition process high spatiotemporal resolution, which crucial for tailoring efficient nanocatalysts diverse applications.
Язык: Английский
Процитировано
10
Analytical Chemistry, Год журнала: 2021, Номер 93(49), С. 16425 - 16431
Опубликована: Ноя. 29, 2021
Electrochemiluminescence (ECL) behavior of luminol derivative was investigated in reduction on different electrode materials. We found that and its widely used L-012 derivative, emitting at physiological pH values, exhibit strong cathodic ECL emission iron stainless steel electrodes with hydrogen peroxide, whereas no signal observed other classic materials (Au, Pt, C). On a Ni electrode, low observed. This points out to the essential role iron-containing enhance emission. Under reported conditions, is comparable classically anodic Thus, dual bright emissions were obtained simultaneously oxidation as imaged wireless bipolar electrochemistry configuration. Such an system generating light concomitantly extremely rare it opens appealing (bio)analytical imaging applications, biosensing, remote detection, analysis, ECL-based cell microscopy.
Язык: Английский
Процитировано
48
Analytical Chemistry, Год журнала: 2021, Номер 93(48), С. 16149 - 16157
Опубликована: Ноя. 18, 2021
Electrochemiluminescence (ECL), as an advanced sensing process, can selectively control the generation of excited states by changing potential. However, most existing ECL systems rely on poisonous coreactants to provide radicals for luminescence; although efficiency was improved, athematic will cause unpredictable interference accurate analysis trace targets. Herein, we realized nonemitting molecules performing intramolecular electron transfer in olefin-linked covalent organic frameworks (COFs), with a high 63.7%. Employing internal dissolved oxygen coreactant, it is well suitable various complex samples environment. Taking nuclear contamination goal orientation, further illustrated design "turn-on" uranyl ion monitoring system integrating fast response, low detection limit, and selectivity, showing that new ECL-COFs are promising facilitate environment-related structure-feature correlation mechanism exploration.
Язык: Английский
Процитировано
44
Analytical Chemistry, Год журнала: 2022, Номер 94(36), С. 12500 - 12506
Опубликована: Авг. 31, 2022
Screening high-performance anodic electrochemiluminescence (ECL) systems with low triggering potential is a promising way to broaden their applications. In addition electrochemiluminophore, co-reactant also plays an important role in the ECL process, since oxidation of co-reactants one most steps process. Herein, novel co-reactant-mediated low-potential Au nanocluster (AuNC)-based system has been successfully developed. Benefiting from isopropyl substitution and hydroxyl triethylamine (TEA), BSA-AuNC/2-(diisopropylamino)ethanol (DIPEA-OH) achieved higher energy efficiency at lower 0.75 V. addition, compared BSA-AuNC/TEA system, intensity quantum yield (ΦECL) DIPEA-OH as increased 22.34-fold 13-fold (as high 68.17%), respectively. Based on potential, ΦECL AuNC/DIPEA-OH sandwich-type immunosensor constructed for highly selective SARS-CoV-2 N protein assay. absence any complex signal amplification strategies, detection showed linear range 0.001–100 ng/mL limit 0.35 pg/mL. Moreover, platform had good reproducibility stability exhibited acceptable performance actual serum samples. This work established framework in-depth design study anode AuNCs other luminophores, expanded application sensors clinical diagnosis COVID-19.
Язык: Английский
Процитировано
32
Biosensors, Год журнала: 2022, Номер 12(7), С. 485 - 485
Опубликована: Июль 3, 2022
In vitro diagnosis (IVD) has become a hot topic in laboratory research and achievement transformation. However, due to the high cost, time-consuming complex operation of traditional technologies, some new technologies are being introduced into IVD, solve existing problems. As result, IVD begun develop toward point-of-care testing (POCT), subdivision field IVD. The pandemic made governments health institutions realize urgency accelerating development POCT. Microfluidic paper-based analytical devices (μPADs), low-cost, high-efficiency, easy-to-operate detection platform, have played significant role advancing μPADs composed paper as core material, certain unique substances reagents for processing paper, sensing devices, auxiliary equipment. published reviews on same lack comprehensive systematic introduction μPAD classification progress segmentation. this we first briefly introduce origin their promoting section. Then, methods summarized, innovative achievements reviewed. Finally, discuss prospect upgrade improvement directions μPADs, terms portability, sensitivity, automation, help researchers clarify overcome difficulties subsequent research.
Язык: Английский
Процитировано
29
Nano Letters, Год журнала: 2023, Номер 23(10), С. 4572 - 4578
Опубликована: Май 12, 2023
In this study, we proposed a novel imaging technique, photoinduced electrogenerated chemiluminescence microscopy (PECLM), to monitor redox reactions driven by hot carriers on single gold nanoparticles (AuNPs) TiO2. Under laser irradiation, plasmon-generated were separated an electric field, leaving holes the surface of AuNPs drive ECL reactions. PECL intensity was highly sensitive number carriers. Through quantitative image analysis, found that density individual decreased significantly with increase in particle diameter, indicating size has significant impact photoelectrochemical conversion efficiency. For first time, verified feasibility PECLM mapping catalytic activity photocatalysts. opens new prospect for situ photocatalysis high-throughput way, which not only facilitates optimization plasmonic photocatalysts but also contributes dynamic study photocatalytic processes micro/nanointerfaces.
Язык: Английский
Процитировано
23