Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(18), P. 7372 - 7378
Published: April 25, 2023
Electrochemiluminescence
(ECL)
microscopy
is
an
emerging
technique
with
new
applications
such
as
imaging
of
single
entities
and
cells.
Herein,
we
have
developed
a
bimodal
bicolor
approach
to
record
both
positive
ECL
(PECL:
light-emitting
object
on
dark
background)
shadow
label-free
(SECL:
nonemissive
shadowing
the
background
luminescence)
images
This
result
simultaneous
emissions
[Ru(bpy)3]2+
used
label
cellular
membrane
(PECL)
[Ir(sppy)3]3–
dissolved
in
solution
(SECL).
By
spectrally
resolving
emission
wavelengths,
recorded
same
cells
PECL
SECL
modes
using
(λmax
=
620
nm)
515
luminescence,
respectively.
shows
distribution
labels
attached
membrane,
whereas
reflects
local
diffusional
hindrance
reagents
by
each
cell.
The
high
sensitivity
surface-confined
features
reported
are
demonstrated
cell–cell
contacts
during
mitosis
process.
Furthermore,
comparison
demonstrates
differential
diffusion
tri-n-propylamine
through
permeabilized
cell
membranes.
Consequently,
this
dual
enables
morphology
adhering
surface
can
significantly
contribute
multimodal
bioassays
different
luminescent
systems.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(15), P. 5852 - 5859
Published: April 1, 2024
A
multicolor
electrochemiluminescence
(ECL)
biosensor
based
on
a
closed
bipolar
electrode
(BPE)
array
was
proposed
for
the
rapid
and
intuitive
analysis
of
three
prostate
cancer
staging
indicators.
First,
[Irpic-OMe],
[Ir(ppy)2(acac)],
[Ru(bpy)3]2+
were
applied
as
blue,
green,
red
ECL
emitters,
respectively,
whose
mixed
emission
colors
covered
whole
visible
region
by
varying
voltages.
Afterward,
we
designed
simple
Mg2+-dependent
DNAzyme
(MNAzyme)-driven
tripedal
DNA
walker
(TD
walker)
to
release
output
DNAs.
Immediately
after,
DNAs
added
cathodic
reservoirs
BPE
incubation.
After
that,
found
that
from
anode
changed
driving
voltage
8.0
V
applied,
mainly
due
changes
in
interfacial
potential
faradaic
currents
at
two
poles
BPE.
Via
optimization
experimental
parameters,
cutoff
values
such
indicators
different
clinical
stages
could
be
identified
instantly
with
naked
eye,
standard
precision
swatches
multiple
prepared.
Finally,
order
precisely
determine
stage,
device
used
analysis,
resulting
images
then
compared
swatches,
laying
way
accurate
therapy.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(4), P. 1651 - 1658
Published: Jan. 19, 2024
The
development
of
a
highly
accurate
electrochemiluminescence
(ECL)
signal
switch
to
avoid
nonspecific
stimulus
responses
is
currently
significant
and
challenging
task.
Here,
we
constructed
universal
utilizing
luminophore–quencher
pair
mesostructured
silica
xerogel-confined
polymer
gold
nanoparticles
(Au
NPs)
that
can
accurately
detect
low-abundance
epigenetic
markers
in
complex
sample
systems.
Notably,
the
ECL
encapsulated
xerogel
acts
as
luminophore,
which
demonstrated
specific
dependence
on
Au
NPs-mediated
energy
transfer
quenching.
To
demonstrate
feasibility,
specifically
labeled
5-hydroxymethylcytosine
(5hmC)
site
random
sequence
using
double-stranded
(dsDNA)
tag
was
skillfully
designed
with
CRISPR/Cas12a
activator
recombinant
polymerase
amplification
(RPA)
template.
After
by
RPA,
large
amount
dsDNA
generated
initiate
trans-cleavage
activity
subsequently
activate
switch,
allowing
for
precise
quantification
5hmC.
improves
stability
luminophore
prevents
responses,
providing
new
paradigm
constructing
high-precision
biosensors.
Nano Letters,
Journal Year:
2024,
Volume and Issue:
24(15), P. 4665 - 4671
Published: April 8, 2024
Effective
bimetallic
nanoelectrocatalysis
demands
precise
control
of
composition,
structure,
and
understanding
catalytic
mechanisms.
To
address
these
challenges,
we
employ
a
two-in-one
approach,
integrating
online
synthesis
with
real-time
imaging
Au@Metal
core–shell
nanoparticles
(Au@M
NPs)
via
electrochemiluminescence
microscopy
(ECLM).
Within
120
s,
electrodeposition
in
situ
activity
screening
alternate.
ECLM
captures
transient
faradaic
processes
during
potential
switches,
visualizes
electrochemical
real-time,
tracks
dynamics
at
the
single-particle
level.
Analysis
using
ECL
photon
flux
density
eliminates
size
effects
yields
quantitative
electrocatalytic
results.
Notably,
nonlinear
trend
corresponding
to
shell
metal
Au
surface
atomic
ratio
is
discerned,
quantifying
optimal
component
Au@M
NPs.
This
approach
offers
comprehensive
behavior
deposition
process
high
spatiotemporal
resolution,
which
crucial
for
tailoring
efficient
nanocatalysts
diverse
applications.
Analytical Chemistry,
Journal Year:
2021,
Volume and Issue:
93(49), P. 16425 - 16431
Published: Nov. 29, 2021
Electrochemiluminescence
(ECL)
behavior
of
luminol
derivative
was
investigated
in
reduction
on
different
electrode
materials.
We
found
that
and
its
widely
used
L-012
derivative,
emitting
at
physiological
pH
values,
exhibit
strong
cathodic
ECL
emission
iron
stainless
steel
electrodes
with
hydrogen
peroxide,
whereas
no
signal
observed
other
classic
materials
(Au,
Pt,
C).
On
a
Ni
electrode,
low
observed.
This
points
out
to
the
essential
role
iron-containing
enhance
emission.
Under
reported
conditions,
is
comparable
classically
anodic
Thus,
dual
bright
emissions
were
obtained
simultaneously
oxidation
as
imaged
wireless
bipolar
electrochemistry
configuration.
Such
an
system
generating
light
concomitantly
extremely
rare
it
opens
appealing
(bio)analytical
imaging
applications,
biosensing,
remote
detection,
analysis,
ECL-based
cell
microscopy.
Analytical Chemistry,
Journal Year:
2021,
Volume and Issue:
93(48), P. 16149 - 16157
Published: Nov. 18, 2021
Electrochemiluminescence
(ECL),
as
an
advanced
sensing
process,
can
selectively
control
the
generation
of
excited
states
by
changing
potential.
However,
most
existing
ECL
systems
rely
on
poisonous
coreactants
to
provide
radicals
for
luminescence;
although
efficiency
was
improved,
athematic
will
cause
unpredictable
interference
accurate
analysis
trace
targets.
Herein,
we
realized
nonemitting
molecules
performing
intramolecular
electron
transfer
in
olefin-linked
covalent
organic
frameworks
(COFs),
with
a
high
63.7%.
Employing
internal
dissolved
oxygen
coreactant,
it
is
well
suitable
various
complex
samples
environment.
Taking
nuclear
contamination
goal
orientation,
further
illustrated
design
"turn-on"
uranyl
ion
monitoring
system
integrating
fast
response,
low
detection
limit,
and
selectivity,
showing
that
new
ECL-COFs
are
promising
facilitate
environment-related
structure-feature
correlation
mechanism
exploration.
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(36), P. 12500 - 12506
Published: Aug. 31, 2022
Screening
high-performance
anodic
electrochemiluminescence
(ECL)
systems
with
low
triggering
potential
is
a
promising
way
to
broaden
their
applications.
In
addition
electrochemiluminophore,
co-reactant
also
plays
an
important
role
in
the
ECL
process,
since
oxidation
of
co-reactants
one
most
steps
process.
Herein,
novel
co-reactant-mediated
low-potential
Au
nanocluster
(AuNC)-based
system
has
been
successfully
developed.
Benefiting
from
isopropyl
substitution
and
hydroxyl
triethylamine
(TEA),
BSA-AuNC/2-(diisopropylamino)ethanol
(DIPEA-OH)
achieved
higher
energy
efficiency
at
lower
0.75
V.
addition,
compared
BSA-AuNC/TEA
system,
intensity
quantum
yield
(ΦECL)
DIPEA-OH
as
increased
22.34-fold
13-fold
(as
high
68.17%),
respectively.
Based
on
potential,
ΦECL
AuNC/DIPEA-OH
sandwich-type
immunosensor
constructed
for
highly
selective
SARS-CoV-2
N
protein
assay.
absence
any
complex
signal
amplification
strategies,
detection
showed
linear
range
0.001–100
ng/mL
limit
0.35
pg/mL.
Moreover,
platform
had
good
reproducibility
stability
exhibited
acceptable
performance
actual
serum
samples.
This
work
established
framework
in-depth
design
study
anode
AuNCs
other
luminophores,
expanded
application
sensors
clinical
diagnosis
COVID-19.
Biosensors,
Journal Year:
2022,
Volume and Issue:
12(7), P. 485 - 485
Published: July 3, 2022
In
vitro
diagnosis
(IVD)
has
become
a
hot
topic
in
laboratory
research
and
achievement
transformation.
However,
due
to
the
high
cost,
time-consuming
complex
operation
of
traditional
technologies,
some
new
technologies
are
being
introduced
into
IVD,
solve
existing
problems.
As
result,
IVD
begun
develop
toward
point-of-care
testing
(POCT),
subdivision
field
IVD.
The
pandemic
made
governments
health
institutions
realize
urgency
accelerating
development
POCT.
Microfluidic
paper-based
analytical
devices
(μPADs),
low-cost,
high-efficiency,
easy-to-operate
detection
platform,
have
played
significant
role
advancing
μPADs
composed
paper
as
core
material,
certain
unique
substances
reagents
for
processing
paper,
sensing
devices,
auxiliary
equipment.
published
reviews
on
same
lack
comprehensive
systematic
introduction
μPAD
classification
progress
segmentation.
this
we
first
briefly
introduce
origin
their
promoting
section.
Then,
methods
summarized,
innovative
achievements
reviewed.
Finally,
discuss
prospect
upgrade
improvement
directions
μPADs,
terms
portability,
sensitivity,
automation,
help
researchers
clarify
overcome
difficulties
subsequent
research.
Nano Letters,
Journal Year:
2023,
Volume and Issue:
23(10), P. 4572 - 4578
Published: May 12, 2023
In
this
study,
we
proposed
a
novel
imaging
technique,
photoinduced
electrogenerated
chemiluminescence
microscopy
(PECLM),
to
monitor
redox
reactions
driven
by
hot
carriers
on
single
gold
nanoparticles
(AuNPs)
TiO2.
Under
laser
irradiation,
plasmon-generated
were
separated
an
electric
field,
leaving
holes
the
surface
of
AuNPs
drive
ECL
reactions.
PECL
intensity
was
highly
sensitive
number
carriers.
Through
quantitative
image
analysis,
found
that
density
individual
decreased
significantly
with
increase
in
particle
diameter,
indicating
size
has
significant
impact
photoelectrochemical
conversion
efficiency.
For
first
time,
verified
feasibility
PECLM
mapping
catalytic
activity
photocatalysts.
opens
new
prospect
for
situ
photocatalysis
high-throughput
way,
which
not
only
facilitates
optimization
plasmonic
photocatalysts
but
also
contributes
dynamic
study
photocatalytic
processes
micro/nanointerfaces.
