Multi-Species Probiotic Strain Mixture Enhances Intestinal Barrier Function by Regulating Inflammation and Tight Junctions in Lipopolysaccharides Stimulated Caco-2 Cells

Microorganisms, Год журнала: 2023, Номер 11(3), С. 656 - 656

Опубликована: Март 3, 2023

Although leaky gut syndrome is not recognized as an official diagnosis for human diseases, it now believed that dysfunction of the cell barrier causes increased permeability intestinal epithelial cells leading to this condition. Probiotics have been widely used improve health, and studies investigated relevance protecting by taking probiotic strains in vitro vivo. However, most restricted use single or several do consider commercially available products composed multi-species. In study, we provide experimental evidence a multi-species mixture eight different heat-treated strain effective preventing conditions. We employed co-culture model system utilizing two differentiated lines mimic tissue. The integrity function was protected preserving occludin protein level activating AMPK signaling pathway, associated with tight junctions (TJs), through treatment Caco-2 cells. Moreover, confirmed application reduced expression proinflammatory cytokine genes inhibiting NFκB pathway when artificial inflammation induced system. Finally, proved measured trans-epithelial electrical resistance (TEER) significantly decreased treated cells, indicating compromised. exhibited protective effect on via enhancing TJ complexes reducing inflammatory responses

Язык: Английский

---

Bifidobacterium bifidum E3 Combined with Bifidobacterium longum subsp. infantis E4 Improves LPS-Induced Intestinal Injury by Inhibiting the TLR4/NF-κB and MAPK Signaling Pathways In Vivo

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(23), С. 8915 - 8930

Опубликована: Май 31, 2023

Changes in the functions of intestinal barrier occur parallel with development sepsis. The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated mice injected lipopolysaccharide (LPS). results revealed an increase ratio ileal villus length to crypt depth BLBB group compared that LPS group, as were number IgA+ plasma, CD4+/CD8+ T, dendritic cells. levels diamine oxidase (DAO) d-lactic acid serum lessened after injection group. In addition, exhibited increased expression level tight junction proteins (zonula occludens-1, occludin, claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, decreased inflammatory cytokines (IL-1β, IL-6, TNF-α). enriched relative abundance Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, Alistipes, resulting producing short-chain fatty acids. Furthermore, leads higher deoxycholic biosynthesized linoleate. This study suggested could enhance capacity mucosal immunity, reduce inflammation, improve composition gut microbiota. bifidum E3 combined longum subsp. infantis E4 may thus serve a probiotic against injury caused LPS.

Язык: Английский

---

Gallic acid attenuates LPS-induced inflammation in Caco-2 cells by suppressing the activation of the NF-κB/MAPK signaling pathway

Acta Biochimica et Biophysica Sinica, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Inflammatory bowel disease (IBD) is a chronic inflammatory characterized by intestinal barrier dysfunction, synergistic effects and excessive tissue injury. Gallic acid (GA) renowned for its remarkable biological activity, encompassing anti-inflammatory antioxidant properties. However, the underlying mechanisms which GA protects against inflammation have not been fully elucidated. The aim of this study to investigate effect on lipopolysaccharide (LPS)-stimulated human colon carcinoma cell line (Caco-2) explore molecular mechanism involved. Our findings demonstrate that 5 μg/mL restores downregulation mRNA protein levels Claudin-1, Occludin, ZO-1 decreases expressions factors such as IL-6, IL-1β TNF-α induced LPS. In addition, exhibits protective reducing LPS-enhanced early late apoptotic ratios, downregulating pro-apoptotic (

Язык: Английский

---

The abundance of bifidobacterium in relation to visceral obesity and serum uric acid

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Июль 29, 2022

Gut microbiome has been shown to play a role in the development of obesity recent studies. Most these studies on were based BMI classification criteria, which doesn't distinguish Visceral adipose tissue (VAT) from subcutaneous (SAT). Some showed that VAT higher risk inducing metabolic diseases than SAT. This study focused visceral defined by increased fat area. The present was designed investigate association with gut predominant microbiota and status. included 372 healthy individuals medical examination center Shulan Hangzhou Hospital. Quantitative polymerase chain reaction (q-PCR) technique used detect ten kinds bacteria fresh feces. area (VFA) measured bioimpedance analyzer (INBODY720, Korea). abundance Bifidobacterium significantly decreased group. Compared lean group, group had levels LDL, TG, FBG, serum uric acid (SUA) lower HDL. SUA an independent impact factor for Bifidobacterium. negatively correlated positively VFA. In mediation analysis, significant effect. may be mediating between VAT.

Язык: Английский

---

Probiotics, their action modality and the use of multi-omics in metamorphosis of commensal microbiota into target-based probiotics

Frontiers in Nutrition, Год журнала: 2022, Номер 9

Опубликована: Сен. 16, 2022

This review article addresses the strategic formulation of human probiotics and allows reader to walk along journey that metamorphoses commensal microbiota into target-based probiotics. It recapitulates what are probiotics, their history, main mechanisms through which exert beneficial effects on host. articulates how a given probiotic preparation could not be all-encompassing each strain has its unique repertoire functional genes. answers criteria should met formulate intended for use, why certain meet ill-fate in pre-clinical clinical trials? communicates reasons taint reputation cause discord between industry, medical scientific communities. revisits notion host-adapted strains carrying niche-specific genetic modifications. Lastly, this paper emphasizes development using microbial isolates with known molecular effectors would serve as better candidates bioprophylactic biotherapeutic interventions disease-susceptible individuals.

Язык: Английский

---

Plant essential oils improve growth performance by increasing antioxidative capacity, enhancing intestinal barrier function, and modulating gut microbiota in Muscovy ducks

Poultry Science, Год журнала: 2023, Номер 102(8), С. 102813 - 102813

Опубликована: Май 25, 2023

Essential oils (EO) are known for their antioxidant, anti-inflammatory, antimicrobial, and growth-promoting properties. However, data rgarding impact on the intestinal health gut microbiota of ducks remain limited. Thus, this study aimed to investigate effects plant EO growth performance, health, Muscovy ducks. A total 360 healthy male aged 1 d were randomly divided into 4 groups with 6 replicates 15 per replicate. Ducks fed basal diets supplemented 0, 100, 200, or 300 mg/kg EO. The results showed that 200 supplementation significantly (P < 0.05) increased final body weight average daily gain, while decreased feed conversion ratio during 56-d experimental period. Furthermore, dietary enhanced antioxidant capacity immune function improved barrier intestine. Additionally, 16S rDNA sequencing analysis favorably modulated cecal microbial diversities composition evidenced by abundances short-chain fatty acid-producing bacteria (e.g., Subdoligranulum Shuttleworthia) potential enteric pathogenic Alistipes, Eisenbergiella, Olsenella). relative abundance beneficial was positively correlated immune, biomarkers. Overall, these findings revealed had several potentially performance improving capacity, enhancing modulating microbiota.

Язык: Английский

---

Phyllanthus emblica Fruit Improves Obesity by Reducing Appetite and Enhancing Mucosal Homeostasis via the Gut Microbiota–Brain–Liver Axis in HFD-Induced Leptin-Resistant Rats

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(18), С. 10406 - 10419

Опубликована: Апрель 25, 2024

The impact of leptin resistance on intestinal mucosal barrier integrity, appetite regulation, and hepatic lipid metabolism through the microbiota–gut–brain–liver axis has yet to be determined. Water extract Phyllanthus emblica L. fruit (WEPE) its bioactive compound gallic acid (GA) effectively alleviated methylglyoxal (MG)-triggered in vitro. Therefore, this study investigated how WEPE GA intervention relieve resistance-associated dysfunction mucosa, appetite, accumulation high-fat diet (HFD)-fed rats. results showed that significantly reduced tissues (jejunum, brain, liver) MG-evoked resistance, malondialdehyde (MDA), proinflammatory cytokines, SOCS3, orexigenic neuropeptides, increasing receptor, tight junction proteins, antimicrobial peptides, anorexigenic excretion fecal triglyceride (TG), short-chain fatty acids (SCFAs) via a positive correlation with Allobaculum Bifidobacterium microbiota. These novel findings suggest holds potential as functional food ingredient for alleviating obesity complications.

Язык: Английский

---

Fermentation enhances the amelioration effect of bee pollen on Caco-2 monolayer epithelial barrier dysfunction based on NF-κB-mediated MLCK-MLC signaling pathway

Food Research International, Год журнала: 2024, Номер 178, С. 113938 - 113938

Опубликована: Янв. 4, 2024

Язык: Английский

---

Taxonomic and phenotypic analysis of bifidobacteria isolated from IBD patients as potential probiotic strains

BMC Microbiology, Год журнала: 2024, Номер 24(1)

Опубликована: Июнь 29, 2024

Abstract Background Inflammatory Bowel Diseases (IBD) are a major public health issue with unclear aetiology. Changes in the composition and functionality of intestinal microbiota associated these pathologies, including depletion strict anaerobes such as Feacalibacterium prausnitzii . Less evidence is observed for other anaerobes, among which bifidobacteria. This study characterized taxonomic functional diversity bifidobacteria isolated from human active non-active IBD patients by culturomics approach evaluated if might be used probiotics gut health. Results A total 341 were (52 Crohn’s disease 26 ulcerative colitis patients), high proportion Bifidobacterium dentium strains (28% bifidobacteria). In colitis, species identified was B. (39% bifidobacteria), colitis. disease, adolescentis (40%), while similar amounts found patients. The relative abundance increased age, both Antibacterial capacities against Escherichia coli LF82 Salmonella enterica ATCC 14028 more often compared to Finally, longum retained highest probiotic potential they presenting exopolysaccharide synthesis, antibacterial activity, anti-inflammatory capacities. Antimicrobial activity EPS synthesis further correlated presence antimicrobial gene clusters silico analysis. Conclusions Different bifidobacterial profiles most abundant , mainly Additionally, significantly age. Furthermore, this evidenced that (antipathogenic production activity), especially strains, can

Язык: Английский

---

Senegalia macrostachya seed polysaccharides attenuate inflammation-induced intestinal epithelial barrier dysfunction in a Caco-2 and RAW264.7 macrophage co-culture model by inhibiting the NF-κB/MLCK pathway

Food & Function, Год журнала: 2022, Номер 13(22), С. 11676 - 11689

Опубликована: Янв. 1, 2022

The intestinal barrier dysfunction associated with chronic inflammation is a major health concern. This work aimed to investigate the protective effect and molecular mechanism of Senegalia macrostachya seed polysaccharide fraction (SMSP2) on inflammation-induced using Caco-2 cells RAW264.7 macrophage co-culture model. results showed that LPS stimulation basolateral compromised integrity apical differentiated monolayer, resulting in decreased transepithelial electrical resistance (TER) increased inflammatory markers. SMSP2 treatment maintained higher TER value reduced epithelial permeability lucifer yellow (LY) dye. In addition, group significant decrease mRNA level factors such as inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), interleukin (IL)-8, tumor necrosis factor-α (TNF-α) monolayer compared group. Moreover, expression tight junctions (TJ) zonula occludens (ZO-1), occludin, claudin-1 at protein levels. Furthermore, immunofluorescence assay ameliorated overall distribution TJ proteins monolayer. application also resulted downregulation nuclear factor kappa-B (NF-κB) phosphorylation myosin light chain kinase (MLCK) expression, which implies preserved from disruption through inhibition NF-kB-mediated MLCK signaling pathway activation. seeds could therefore be promising functional food used improve function.

Язык: Английский

---