Biosensors,
Год журнала:
2022,
Номер
12(5), С. 300 - 300
Опубликована: Май 5, 2022
Surface-enhanced
Raman
scattering
(SERS)
spectroscopy
is
a
surface-
or
cavity-enhanced
variant
of
that
allows
the
detection
analytes
with
sensitivity
down
to
single
molecules.
This
method
involves
use
SERS-active
surfaces
cavities
capable
concentrating
incident
radiation
into
small
mode
volumes
containing
analyte.
Here,
we
have
engineered
an
ultranarrow
metal-dielectric
nano-cavity
out
film
receptor-binding
domain
(RBD)
SARS-CoV-2
spike
(S)
glycoprotein
and
silver
surface,
held
together
by
interaction
between
reduced
protein
sulfhydryl
groups
silver.
The
concentration
light
in
this
label-free
recording
characteristic
spectra
samples
smaller
than
1
pg.
sufficient
for
ultrasensitive
viral
antigens
at
physiologically
relevant
levels.
Moreover,
SERS
signal
can
be
increased
several
orders
magnitude
coating
RBD
nanometer-thick
shell,
thereby
raising
cavity
Q-factor.
ensures
sub-femtogram
antigen
detection.
A
simple
theoretical
model
explaining
observed
additional
enhancement
from
silver-coated
proposed.
Our
study
first
obtain
S
glycoprotein,
key
antigen,
directly,
without
Raman-reporter
Thus,
our
approach
concentrations
lower
those
required
detecting
whole
virus
biological
media.
makes
it
possible
develop
high-performance
optical
conformational
analysis
pathogen
its
variants.
Journal of Biophotonics,
Год журнала:
2023,
Номер
16(7)
Опубликована: Март 31, 2023
Abstract
The
fast
spread
and
transmission
of
the
coronavirus
2019
(COVID‐19)
has
become
one
serious
global
public
health
problems.
Herein,
a
surface
enhanced
Raman
spectroscopy‐based
lateral
flow
immunoassay
(LFA)
was
developed
for
detection
SARS‐CoV‐2
antigen.
Using
uniquely
designed
core–shell
nanoparticle
with
embedded
probe
molecules
as
indicator
to
reveal
concentration
target
protein,
excellent
quantitative
performance
limit
(LOD)
0.03
ng/mL
range
10–1000
can
be
achieved
within
15
min.
Besides,
spiked
virus
protein
in
human
saliva
also
performed
portable
spectrometer,
proposing
feasibility
method
practical
applications.
This
easy‐to‐use,
rapid
accurate
would
provide
point‐of‐care
testing
way
ideal
alternative
current
requirement
virus‐related
biomarkers.
Biotechnology Journal,
Год журнала:
2023,
Номер
19(1)
Опубликована: Сен. 26, 2023
Abstract
Non‐infectious
virus‐like
particles
(VLPs)
are
excellent
structures
for
development
of
many
biomedical
applications
such
as
drug
delivery
systems,
vaccine
production
platforms,
and
detection
techniques
infectious
diseases
including
SARS‐CoV‐2
VLPs.
The
characterization
biochemical
biophysical
properties
purified
VLPs
is
crucial
methods
therapeutics.
presence
spike
(S)
protein
in
their
structure
especially
important
since
S
induces
immunological
response.
In
this
study,
a
rapid,
low‐cost,
easy‐to‐use
technique
both
the
two
VLPs,
which
HIV‐based
was
achieved
using
surface‐enhanced
Raman
spectroscopy
(SERS).
To
analyze
classify
datasets
SERS
spectra
obtained
from
VLP
groups,
machine
learning
classification
support
vector
(SVM),
k‐nearest
neighbors
(kNN),
random
forest
(RF)
were
utilized.
Among
them,
SVM
algorithm
demonstrated
best
performance
groups
with
87.5%
92.5%
accuracy,
respectively.
This
study
could
be
valuable
rapid
novel
therapeutics
or
structural
proteins
viruses
leading
to
variety
diseases.
Biomedical Optics Express,
Год журнала:
2023,
Номер
14(8), С. 4097 - 4097
Опубликована: Июнь 30, 2023
The
structure
of
the
SARS-CoV-2
spike
RBD
and
human
ACE2
as
well
changes
in
due
to
binding
activities
were
analysed
using
surface
enhanced
Raman
spectroscopy.
inhibitor
cohaerin
C
was
applied
inhibit
between
ACE2.
Differences
spectra
determined
deconvolution
amide
bands
principal
component
analysis.
We
thus
demonstrate
a
fast
label-free
analysis
protein
structures
differentiation
bound
unbound
states.
approach
is
suitable
for
sensing
screening
might
be
relevant
investigate
other
systems
well.
