Bone, Год журнала: 2024, Номер unknown, С. 117383 - 117383
Опубликована: Дек. 1, 2024
Язык: Английский
Bone, Год журнала: 2024, Номер unknown, С. 117383 - 117383
Опубликована: Дек. 1, 2024
Язык: Английский
Food Bioscience, Год журнала: 2025, Номер unknown, С. 106785 - 106785
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0Foods, Год журнала: 2023, Номер 12(5), С. 1075 - 1075
Опубликована: Март 2, 2023
Mesenchymal stem cells (MSCs) can be used as a cell source for cultivated meat production due to their adipose differentiation potential, but MSCs lose stemness and undergo replicative senescence during expansion in vitro. Autophagy is an important mechanism senescent remove toxic substances. However, the role of autophagy controversial. Here, we evaluated changes porcine (pMSCs) long-term culture vitro identified natural phytochemical, ginsenoside Rg2, that could stimulate pMSC proliferation. First, some typical characteristics were observed aged pMSCs, including decreased EdU-positive cells, increased senescence-associated beta-galactosidase activity, declined stemness-associated marker OCT4 expression, enhanced P53 expression. Importantly, autophagic flux was impaired suggesting deficient substrate clearance pMSCs. Rg2 found promote proliferation pMSCs using MTT assay EdU staining. In addition, inhibited D-galactose-induced oxidative stress activity via AMPK signaling pathway. Furthermore, with promoted proliferation, senescence, maintained These results provide potential strategy MSC
Язык: Английский
Процитировано
8The FASEB Journal, Год журнала: 2023, Номер 37(10)
Опубликована: Сен. 28, 2023
Brain aging is the most important risk factor for neurodegenerative disorders, and abnormal apoptosis linked to neuronal dysfunction. Specifically, studies have found that exercise effectively inhibits hippocampal apoptosis, while molecular mechanism remains unclear. In present study, we investigated impact of aerobic on in mice potential involvement DAPK1 its downstream pathways based recent data may be associated with death diseases. Senescent were subjected 8 weeks Aerobic training. Following behavioral testing, samples examined histologically biochemically detect pathological changes, mRNA protein levels. We intervention improved spatial memory alleviated brain. Notably, down-regulated expression inhibited Fas receptor transactivation mitochondrial apoptotic pathway hippocampus. These results shed new light protective effect regular against brain though modulating pathway.
Язык: Английский
Процитировано
7Inflammation Research, Год журнала: 2023, Номер 72(3), С. 603 - 621
Опубликована: Янв. 24, 2023
Язык: Английский
Процитировано
6Current Medical Science, Год журнала: 2024, Номер 44(4), С. 748 - 758
Опубликована: Июнь 20, 2024
Язык: Английский
Процитировано
2Journal of Liposome Research, Год журнала: 2023, Номер 34(3), С. 435 - 451
Опубликована: Ноя. 30, 2023
As the aging population continues to increase, aging-related inflammation, oxidative stress, and neurodegenerative diseases have become serious global health threats. Resveratrol, a star molecule in natural polyphenols, has been widely reported physiological activities such as anti-aging, anti-inflammatory, antioxidant, neuroprotection. However, its poor water solubility, rapid metabolism, low bioavailability targeting ability, which limits application. Accordingly, brain-targeted resveratrol liposome (ANG-RES-LIP) was developed solve these issues. Experimental results showed that ANG-RES-LIP uniform size distribution, good biocompatibility, drug encapsulation rate of over 90%. Furthermore,
Язык: Английский
Процитировано
3Journal of Food Biochemistry, Год журнала: 2024, Номер 2024(1)
Опубликована: Янв. 1, 2024
In neurodegenerative diseases, the activation of microglia and ensuing neuroinflammation are pivotal in regulating disease progression. Attenuating inflammation induced by microglial cells is considered a key strategy for slowing progression diseases. γ‐glutamylcysteine (γ‐GC) has exhibited significant antioxidative anti‐inflammatory effects; nevertheless, its potential role modulating neuroinflammatory responses remains incompletely explored. The current investigation aimed to establish model stimulating BV2 with lipopolysaccharide (LPS) explore protective effect γ‐GC on cells. results demonstrated that significantly attenuated LPS‐induced oxidative damage cells, reduced levels tumor necrosis factor‐α (TNF‐α) interleukin‐1β (IL‐1β), inhibited cytoplasmic translocation high‐mobility group box 1 protein (HMGB1), effectively mitigated inflammatory response We further investigated regulatory mechanism found enhances autophagy resulting marked reduction mammalian target rapamycin (mTOR) phosphorylation an increase AMP‐activated kinase (AMPK) levels. use inhibitors 3‐methyladenine (3‐MA) AMPK corroborates proposition promotes while suppressing through AMPK‐mTOR pathway. findings indicate exerts substantial inhibitory impact neuroinflammation, making it promising candidate development therapeutic strategies against disorders related conditions.
Язык: Английский
Процитировано
0Food Research International, Год журнала: 2024, Номер 198, С. 115357 - 115357
Опубликована: Ноя. 19, 2024
Язык: Английский
Процитировано
0Journal of Food Biochemistry, Год журнала: 2024, Номер 2024(1)
Опубликована: Янв. 1, 2024
The formation and accumulation of foam cells within the arterial wall is main early event atherosclerosis. Vascular smooth muscle (VSMCs) are most abundant in human atherosclerotic lesions suggested to contribute atheroma cells. γ ‐glutamylcysteine ( ‐GC), as an immediate precursor glutathione, exhibits anti‐inflammatory antioxidant effects. However, effects ‐GC on plaques not yet clarified. Here, we display against ox‐LDL‐induced foamy VSMCs. Our results showed that apparently reduced total intracellular cholesterol content mouse rat oil red O fluorescent BODIPY staining indicated inhibited cell. Mechanistically, expression macrophage scavenger receptor 1 (Msr1) VSMCs attenuate uptake by Further RNA‐seq analysis, immunofluorescence well immunoblotting measurements thioredoxin‐1 (Txn1) was important for reduce Msr1 lipid Overall, our research increases Txn1 expression, which causes A reduction inhibition ox‐LDL‐stimulated study revealed has potential prevention
Язык: Английский
Процитировано
0Bone, Год журнала: 2024, Номер unknown, С. 117383 - 117383
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
0